The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a proteome- and phospho-proteome-wide mass spectrometry profiling. We compared protein dynamics with gene transcription, revealing many transcriptionally regulated G2 mRNAs that only produce a protein shift after mitosis. Integration of CRISPR/Cas9 survivability studies further highlighted proteins essential for cell viability. Analyzing the dynamics of phosphorylation events and protein solubility dynamics over the cell cycle, we characterize predicted phospho-peptide motif distributions and predict cell cycle-dependent translocating proteins, as exemplified by the S-adenosylmethionine synthase MAT2A. Our study implicates this enzyme in translocating to the nucleus after the G1/S-checkpoint, which enables epigenetic histone methylation maintenance during DNA replication. Taken together, this data set provides a unique integrated resource with novel insights on cell cycle dynamics.

28 April 2020

This paper aims to assist the region’s local authorities, and their key foreign backers, in understanding how transitional justice can provide alternative avenues for holding local ISIS members to account while contributing to the healing of communities.

The problem occurs on a content release on the SAS Risk Governance Framework.

The problem occurs on a content release on the SAS Risk Governance Framework.

You might see the following error in SAS Workflow Studio when you try to open the CECL_Cycle_AFS workflow template that is shipped with SAS Solution for CECL:

Mutations in retinaldehyde-binding protein 1 (RLBP1), encoding the visual cycle protein cellular retinaldehyde-binding protein (CRALBP), cause an autosomal recessive form of retinal degeneration. By binding to 11-cis-retinoid, CRALBP augments the isomerase activity of retinoid isomerohydrolase RPE65 (RPE65) and facilitates 11-cis-retinol oxidation to 11-cis-retinal. CRALBP also maintains the 11-cis configuration and protects against unwanted retinaldehyde activity. Studying a sibling pair that is compound heterozygous for mutations in RLBP1/CRALBP, here we expand the phenotype of affected individuals, elucidate a previously unreported phenotype in RLBP1/CRALBP carriers, and demonstrate consistencies between the affected individuals and Rlbp1/Cralbp−/− mice. In the RLBP1/CRALBP-affected individuals, nonrecordable rod-specific electroretinogram traces were recovered after prolonged dark adaptation. In ultrawide-field fundus images, we observed radially arranged puncta typical of RLBP1/CRALBP-associated disease. Spectral domain-optical coherence tomography (SD-OCT) revealed hyperreflective aberrations within photoreceptor-associated bands. In short-wavelength fundus autofluorescence (SW-AF) images, speckled hyperautofluorescence and mottling indicated macular involvement. In both the affected individuals and their asymptomatic carrier parents, reduced SW-AF intensities, measured as quantitative fundus autofluorescence (qAF), indicated chronic impairment in 11-cis-retinal availability and provided information on mutation severity. Hypertransmission of the SD-OCT signal into the choroid together with decreased near-infrared autofluorescence (NIR-AF) provided evidence for retinal pigment epithelial cell (RPE) involvement. In Rlbp1/Cralbp−/− mice, reduced 11-cis-retinal levels, qAF and NIR-AF intensities, and photoreceptor loss were consistent with the clinical presentation of the affected siblings. These findings indicate that RLBP1 mutations are associated with progressive disease involving RPE atrophy and photoreceptor cell degeneration. In asymptomatic carriers, qAF disclosed previously undetected visual cycle deficiency.

1 April 2012 , Volume 68, Number 2

In the circular economy nothing is wasted. Repair, not replace, is the byword. Felix Preston on a 30-year-old idea whose day is about to dawn

To evaluate bicycle passing distances in the Australian Capital Territory (ACT), specialised passing distance measurement devices (PDMDs) were installed on a sample of 23 cyclists who ride in the ACT. Passing distance data and GPS data was collected by cyclists using the PDMDs for a four week period, during a trial phase of a newly legislated minimum passing distance (MPD) rule The MPD rule requires drivers to provide more than 1 metre of space when passing a cyclist on a road with a speed limit of 60 km/h or below, and 1.5 meters of space when passing a cyclist on a road with a speed limit above 60 km/h.-- Abstract.

Lecture by Claudio Borio, Head of the Monetary and Economic Department, at the University of Zürich, Zürich, 19 November 2019.

The city's residents sorted waste materials for reuse in future projects, according to new research

Recent literature has highlighted that international trade is mostly priced in a few key vehicle currencies, and is increasingly dominated by intermediate goods and global value chains (GVCs). Taking these features into account, this paper reexamines the business cycle dynamics of international trade and its relationship with monetary policy and exchange rates.

Bank for International Settlements BIS Working Papers by David Cook and Nikhil Patel

Ride 2 Transform takes its third tour of southern Malawi for ministry and support.

TEMPORARY walking and cycling routes are set to pop-up across Scotland in a bid to improve social distancing while Scots are now being advised to wear face coverings in “enclosed spaces”.

Penn State Shenango's Upcycled Art Contest will be held in conjunction with the campus' annual Earth Fest on Saturday, April 4.

Previous studies show beneficial effects of cycled lighting in neonatal care on infant day–night activity, sleep behavior, and postnatal growth. The American Academy of Pediatrics and the American College of Obstetricians and Gynecologists recommend neonatal care under cycled lighting conditions.

This study found that cycled lighting during neonatal care reduces infant’s fussing and crying behavior at 5 and 11 weeks’ corrected age and improves growth during neonatal period. These findings support the introduction of cycled lighting in neonatal care practice. (Read the full article)

Tricycle riding is a common activity in children. In calendar year 2012, tricycle accidents were the most common cause of reported toy-related deaths in children. Little research has been conducted regarding tricycle-related injuries and how to counsel parents appropriately.

This study uses nationally representative data to investigate various characteristics of tricycle-related injuries in children to better counsel parents. This study highlights the importance of helmet and elbow pad use and parental supervision. (Read the full article)

Winners to qualify automatically for UEFA Champions League while commercial rights are fully centralised from group stage

One girl’s dream comes true, as she is now able to go to a village primary school, started by OM.

Massive distribution efforts by OM teams in Bangladesh over more than 10 years sowed gospel seeds and made the Bengali New Testament a #1 best seller.

Revolt RV 400 has finally been launched in India. With features like smartphone connectivity and multiple exhaust sounds, the RV 400 is definitely in a segment of its own. But what is an electric motorcycle to ride like? How does it compare with conventional motorcycles? Abhilasha Singh test rode the RV 400 to answer these […]

Denmark has come up with a route which is only dedicated to the cycle riders. The government is keen on promoting cycling as a major mode of transportation. It will ensure that the level of pollution is bought down and also a livelier and healthier environment…

'New type of analysis' deployed to determine plastics' toxicity

The Revolt RV400 has got the juice and the looks for city riding, but the looks are heavily inspired by a certain Chinese motorcycle

Sets a new record for ‘highest fuel efficiency on a motorcycle’ at 76.4kpl

EICMA 2019 is running in full swing and the past few hours have seen the unveiling of some of the most exciting and awaited motorcycles on the planet. Here we have picked the top five that will be launched in India in the coming months.

Hello,

Would anyone know how to setup a PSS or QPSS simulation with 25% dutycycle clock sources or if such a thing is possible with QPSS.

Fig1 (below) is a snapshot of the circuit I am trying to characterize. This has 4 clock ports each with 25%duty cycle in the ON state. Fig2 below shows two of these clocks.

Each path in the circuit consists of two switches with a low pass RC sandwiched in between. The Input is a 50Ohm port sine wave and the output is a 1K resistor. The output nets of all paths are connected together.

I am trying to determine the swept frequency response from input to output (voltage) when the input is from 500Mhz to  510MHz. The Period (T=1/Fp) of each of the pulses is such that Fp=500MHz. The first pulse source has a delay=0, second has delay=T/4, third delay=2T/4, etc...

I am currently getting it working and seeing the correct result (bandpass response) with Transient but the problem is doing a dft at 500MHz with 10KHz spacings needs at least 100us and takes up a lot of time and disk space.

Many Thanks,
Chris.

Fig1

Fig2

Several reports this week agree that cleantech investments fell in 2013, but that's not a bad thing. And the big question is what happens next: how much more is needed?

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While today's smartphones are loaded with Google Maps or Apple Maps, they are merely quick solutions for traveling short distances. Believe it or not, getting a dedicated GPS device for your car or truck is still the best way to get to your destination safely and with ease. 

Companies like Garmin and TomTom specialize in safe car travel and update their maps and traffic reports whenever changes occur on the road. GPS devices also offer the peace of mind that you'll never get lost, even when you're in a new location — including remote areas. They can also help you find the nearest restaurants, shopping mall, rest area, and other locations that might be considered off-the-beaten-path.  Read more...

By Chimaobi Nwaiwu – Nnewi The Anambra State Commissioner for Transport, Dr. Christian Madubuko has warned Commercial Tricycle Owners and Riders Welfare Association of Anambra State, CTORWAAS, and their alleged paid agents to desist from peddling false allegations against his Ministry or face the wrath of the law. Dr. Madubuko also warned CTORWAAS, its Chairman,
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The post Anambra Commissioner warns tricycle operators against peddling falsehood appeared first on Vanguard News.

Narrated by Zhang Minghong I walked into the studio of the Beijing TV on October 30, 2019 and participated in the recording of a TV program to talk about my life after retirement from the Chinese military over 30 years ago. In fact, I am a very ordinary Chinese veteran. I have been retired from the Chinese People's Liberation Army (PLA) for nearly 30 years and have been earning a living by loading and unloading and riding a tricycle in Ya'an, a city in the western part of China's Sichuan Province. During this time, I haven't done anything incredible. I think the reason why the TV station invited me to participate in the show has to do with the belief that I have persisted for so many years....

Since tea cultivation began around 800 years ago in what’s now Shizuoka Prefecture, it has been renowned as Japan’s largest tea-producing region. Benefitea repackages the ...