Methods and compositions are described for categorizing and treating autoimmune disease, using single cell network profiling (SCNP), where activation levels of one or more activatable elements are determined in single cells, with or without modulation, to categorize or determine treatment for the autoimmune disease.

The invention concerns soluble and antigenic HTLV p24 variants that can be fused to chaperones and their use in diagnostic applications such as immunoassays for detecting antibodies against HTLV-I or HTLV-II in an isolated biological sample. In particular, the invention relates to a soluble HTLV-I or HTLV-II p24 antigen comprising either the N- or the C-terminal domain of p24 and lacking the other domain. Moreover, the invention covers recombinant DNA molecules encoding these HTLV-I and -II fusion antigens as well as their recombinant production using expression vectors and host cells transformed with such expression vectors. In addition, the invention focuses on compositions of these HTLV p24 antigens with HTLV gp21 antigen and on an immunoassay method for detection of HTLV antibodies using the antigens of the invention. Also the use of HTLV p24 antigens in an in vitro diagnostic assay as well as a reagent kit for detection of anti-HTLV-antibodies comprising said HTLV antigens is encompassed.

This invention publicizes a chronic renal disease diagnostic kit, comprised of FGF23 monoclonal antibody, biotin labeling reagent Sulfo-NHS-LC-Biotin, PBS buffer solution (pH7.0, 0.1M), casein saline solution, streptomycin avidin and substrate TMB. The labeling condition is the weight ratio of FGF23 antibody and Sulfo-NHS-LC-Biotin, which is 1:5-1:12. The dilution ratio of streptomycin avidin is 1:3000. The lowest testing threshold limit value of FGF23 kit is 10 pg/ml, whose sensitivity is 10 times higher than that of common monoclonal antibody, which is very significant for early diagnose of chronic renal disease.

The present invention relates to in vitro methods of assessing the susceptibility or responsiveness of a subject to the treatment with an epidermal growth factor receptor (EGFR) inhibitor/antagonist, wherein the subject has been diagnosed or suspected of suffering from inflammation-associated. These methods comprise determining the level of expression of EGFR in myeloid cells in a sample from the subject, wherein an expression of EGFR in the myeloid cells is indicative of the subject being susceptible to the treatment with an EGFR inhibitor/antagonist. The invention also relates to EGFR inhibitors/antagonists for use in the treatment or amelioration of inflammation-associated cancer. The invention furthermore provides in vitro methods of prognosing the survival time, progression-free survival time or disease course of a subject that has been diagnosed or suspected of suffering from from inflammation-associated cancer. In addition thereto, the invention relates to in vitro diagnostic methods of assessing the proneness of a subject to develop inflammation-associated cancer in which the expression of EGFR is determined in myeloid cells from the subject.

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.

A method to detect prostate cancer comprising contacting a sample of prostate cells from the patient with a set of detectably labeled probes under hybridization conditions and determining the presence of chromosomal abnormalities in prostate tumor tissue, PIN (intra-epithelial neoplasia), histologically benign tissue and benign prostatic hyperplasia (BPH); a method to combine immunofluorescence and FISH (IF-FISH) to facilitate the assessment of chromosomal abnormalities; a set of probes; and a kit comprising the set of probes and instructions for diagnosing prostate cancer in a patient.

In sporadic Alzheimer's disease, neurofibrillary lesion formation is preceded by extensive post-translational modification of the microtubule associated protein tau. Immunoassays have been developed recently that detect tau in biological specimens, thus providing a means for pre-mortem diagnosis of Alzheimer's disease, which has remained elusive. These assays have been improved by the analysis of relevant post-translational modifications, such as phosphorylation, however opportunity for improvement remains. The present invention addresses this issue by disclosing synthetic methylated peptides derived from the tau protein of paired helical filaments and non-diseased control brain. Alzheimer's disease specificity is provided by the presence or absence of methyl moieties on lysine residues and differences between mono-, di-, and tri-methylation. The methylated peptide is useful as an antigen and a binding partner for identifying compounds that interact with the peptide and the methylated tau protein, including antibodies that can distinguish non-diseased brain from that affected by Alzheimer's disease. The resulting antibodies are useful diagnostically and therapeutically. The compounds that specifically bind to methylated tau proteins are useful for eliminating abnormally methylated tau.

Oakland's alaMar Kitchen & Bar is about to start serving 1,500 free meals per week to restaurant workers who have been displaced and derailed by the coronavirus outbreak.

Located at 100 Grand Ave., chef Nelson German's popular seafood restaurant has just issued this announcement:

"We are here for you, Oakland! ...…

Off the Grid will stage a two-part free-meal giveaway today, April 8, from 12 noon to 1 p.m. at Nob Hill Foods Alameda and from 2:30 p.m. to 3:30 p.m. at Pak 'N Save Emeryville.

It's part of the Grocery Employees Meal Fund, which OTG recently launched to help creators feed grocery-store employees and their family members throughout the Covd-19 crisis.…

The owners of Oakland's Homestead Restaurant have just debuted a new business concept that is meant to outlast the Covid-19 crisis.

Currently operating at the restaurant's 4029 Piedmont Ave. space Tuesdays through Saturdays from 10 a.m. to 6 p.m. via curbside pickup and Caviar or Doordash delivery, The Humble Sandwich comprises a selection of classic deli sandos made with the same high-quality ingredients for which the seven-year-old restaurant is revered.…

The owners of Oakland's Homestead Restaurant have debuted a new concept meant to outlast the COVID-19 crisis.

East Bay resident Miles Woodruff, CEO of the pioneering vegan-seafood brand Sophie's Kitchen, has just donated 3,000 cases of plant-based Toona to the Food Bank of Contra Costa and Solano County as well as several other Bay Area food shelters.

Amidst the Covid-19 outbreak, the company's brand-new Lemon Grass Toona flavor has not yet arrived in grocery stores — but 100 percent of it is now being provided to people in need.…

Now through May 5, healthcare workers and first responders can collect free "Thank You Meals" at any McDonald's location nationwide.

The promotion began on April 22.…

Here's a leaked track from Oakland vocalist Karyn Paige.

Stories you shouldn’t miss:

1. Alameda County Public Health Director Muntu Davis contends that gentrification should be examined in terms of health impacts because of the displacement and stress it causes for low-income residents, the Bay Area News Group$ reports.…

A ground fault circuit interrupter (GFCI) breaker testing system can include an enclosure having at least one wall that forms a cavity. The system can also include at least one GFCI breaker disposed within the cavity. The system can further include a sensing circuit assembly having at least one switch, where the at least one switch is electrically coupled to the at least one GFCI breaker. The system can also include a user interface assembly disposed, at least in part, outside the cavity, where the user interface assembly is coupled to the sensing circuit assembly, where the user interface assembly instructs the at least one switch to test the at least one GFCI breaker.

Electronic device heat transfer technology is disclosed. In an example, an electronic device package can include a substrate. The electronic device package can also include a heat transfer component. The electronic device package can further include a heat-generating electronic component coupled to the substrate between the substrate and the heat transfer component. The electronic device package can also include a viscous thermal interface material (TIM) providing a heat transfer pathway between the electronic component and the heat transfer component. In addition, the electronic device package can include a barrier about at least a portion of a periphery of the viscous TIM to maintain the viscous TIM within a confined location in proximity to the electronic component. The TIM is uninterrupted by the barrier within the periphery.

Examples of a thermal management unit and an electronic apparatus utilizing the thermal management unit are described. In one aspect, the thermal management unit includes a heat sink. The heat sink includes a base portion, a first protrusion structure and a second protrusion structure. The base portion has a first side and a second side opposite the first side. The first protrusion structure protrudes from the first side of the base portion, and includes multiple fins. The second protrusion structure protrudes from the second side of the base portion, and includes multiple ribs. The heat sink may be made of silicon.

News, arts, and dining coverage for Oakland, Berkeley, and the greater East Bay.

News, arts, and dining coverage for Oakland, Berkeley, and the greater East Bay.

I was wondering why the iconic Civic Centre clock in Southampton is not lit up blue on Thursday at 8pm to clap for the NHS.

CHRISTINE Cassell (letters 28th April) asks a pertinent question when she enquires where the responsibility for the problem with PPEs for front line NHS workers lies.

75 YEARS AGO this week St John Ambulance volunteers played a vital role delivering first aid and providing support to both the public and the armed forces on VE Day.

I SHOULD like to pay tribute to Alan Wiltshire the Echo and Romsey Advertiser reporter whose death was sadly announced last week

ONLY a delusional Lib Dem like Martin Kyrle could come up with an excuse like this about his beloved party doing well in the last election when in fact they lost over half their seats, then blaming it on our undemocratic electoral system, it’s the system we have, and our MP’s are elected on that system.

HE MAY NOT be held in the highest regard by the public but there’s something to love about Piers Morgan on Good Morning Britain.

More rose-tinted views of the EU: the EU not falling apart?

I say to all communities pat yourselves on the back for the great job you are doing taking care of each other and supporting each other.

The achievement of 75 years without any wars between European countries is unprecedented in history.

Weeks have passed, and DJs the world over have settled into the reality of sheltering in place. As efforts to combat COVID-19 push on, the masses are staying at home. Clubs have shuttered their doors, bars and restaurants are no more, and non-essential businesses are closed. But in this primetime of social distancing, there has […]

The post Streaming L(o)ive In The Time of COVID-19: What’s the future for DJ live streaming? appeared first on DJ TechTools.

The live streaming scene might feel crowded, but this week Mixcloud has introduced their own endeavor. They’ve launched a streaming platform built specifically for DJs, Mixcloud Live—not only taking on streaming giants like Facebook, Twitch, and Youtube, but also creating much-needed relief for DJ streams that are continually flagged for copyright. The team at Mixcloud […]

The post Mixcloud Launches A DJ Live Streaming Platform With Copyright Figured Out appeared first on DJ TechTools.

On Facebook, live streaming just got taken things a step further: with monetization. Many suspected monetizing virtual events was coming, and it seems to be rapidly arriving on the largest social network. Facebook has come forward with new updates to assert its dominance in this COVID-19 era of online entertainment and relationships. New Facebook Tools […]

The post Facebook Now Lets You Charge Entry To Livestream Events appeared first on DJ TechTools.

The provocative A Human Ounce at Central Works asks whether the creator can be separate from the creation. Central Works opens its 30th anniversary season with A Human Ounce, a new play by Nicole Parizeau that poses provocative questions. What is the value of great art, literature, music, commerce, scientific discovery — even Peet's coffee — when scandalous information about its creators or owners comes to light?…

The Town’s soccer franchise returns to the pitch this weekend with a new head coach and other fresh faces, kicking off its second season in front of Oakland's loud, loyal fans at Laney College. Last year, professional soccer planted seeds in Oakland. This year, fans hope to see them bloom into something beautiful, preferably with lots of wins on the Laney College pitch.…

Plus what should I do about my cast fetish? I'm a middle-aged gay man and I was recently diagnosed with sleep apnea. This is a disorder caused by the soft tissue in the throat collapsing during sleep.…

Plus, I have no interest in my husband now that he is smoking again. I'm a cis bi woman, and I mainly have sex with people with penises. I have a really gross problem, sorry.…

Coming on the heels of the AB5 trauma, the coronavirus lockdown poses an extra economic threat. The COVID-19 pandemic has affected all facets of life in California and the world.…

A reading list specifically appropriate to these trying times. Okay, you're stuck in the house, but is it really that awful? OK, probably.…

Buy it From Local Stores Harvard Business School professor Ryan Raffaelli describes the unique value that independent booksellers provide to local economies in this way: "It's about community, it's about curation and it's about convenience." Author of a working paper titled, "Reinventing Retail: The Novel Resurgence of Independent Bookstores," Raffaelli argues that in focusing on the "Three Cs" of community, curation, and convenience, independent booksellers have regained their footing in the face of competition from big chains and Amazon and enjoyed a resurgence in recent years.…

A gas separation process for treating exhaust gases from combustion processes. The invention involves routing a first portion of the exhaust stream to a carbon dioxide capture step, while simultaneously flowing a second portion of the exhaust gas stream across the feed side of a membrane, flowing a sweep gas stream, usually air, across the permeate side, then passing the permeate/sweep gas back to the combustor.

An exhaust treatment system comprising a first oxidation catalyst to oxidise nitrogen and/or carbon compounds in an exhaust stream and a first dosage device downstream of said first oxidation catalyst to supply a first additive. A first reduction catalyst device is arranged downstream of said first dosage device for reduction of nitrogen oxides using said first additive, and for the generation of heat, through at least one exothermal reaction with said exhaust stream. A particulate filter arranged downstream of said first reduction catalyst device to catch soot particles and a second dosage device, arranged downstream of said particulate filter to supply a second additive. A second reduction catalyst device is arranged downstream of said second dosage device for reduction of nitrogen oxides in said exhaust stream, through the use of at least one of said first and said second additive.

The invention relates to a storage tank (1) in a motor vehicle for receiving an aqueous urea solution for the SCR of nitrogen oxide in the exhaust gas. The storage tank (1) comprises a tank body which forms a storage volume (2) for the urea solution. The storage tank (1) furthermore comprises a filling pipe for filling the storage volume (2) and means for venting the storage volume (2) during the refuelling, wherein the filling pipe (3) has a filling head (9) at a remote end, and the filling head (9) forms an orifice stub (10). The orifice stub (10) defines an orifice (11) for receiving a fuel pump nozzle. Said orifice stub furthermore has an external thread for receiving a complementary union thread of a refuelling cylinder for refuelling by the gas displacement method. The orifice stub forms at least one secondary air opening when the refuelling cylinder is attached, and therefore, when a refuelling cylinder is attached, a sealing seat is bridged by the refuelling cylinder and refuelling by the gas displacement method is possible even if counterflow venting through the filling tube (3) does not take place.

A catalytic converter for an internal combustion engine includes a tubular member which defines a volume within which a catalytic converter substrate is located, the volume communicates with an inlet portion for receiving exhaust gas emissions and with a first outlet portion for discharging emissions after catalytic conversion. The catalytic converter may also include a pipe member within the tubular member, which connects the inlet portion with the volume and guides emissions from the inlet portion in a first direction. The pipe member opens into a deflector member which deflects emissions into the volume in a second direction, and the catalytic converter includes a second outlet portion connected to the deflector member and a valve to control gas flow through the second outlet portion to guide emissions away from the pipe member and out of the catalytic converter prior to reaching the catalytic converter substrate when the valve is open.

Various embodiments of a waste heat recovery and conversion system are disclosed. The system may include a modular heat exchanger whose energy source is provided by waste heat energy transporting fluids transferring their energy to a working fluid. The working fluid may be in a liquid state contained in a reservoir hydraulically connected to a high-pressure heat transfer chamber. The high-pressure heat transfer chamber may be configured to receive thermal energy utilized to convert the working fluid into a superheated vapor. The system may also include a waste heat conversion system hydraulically connected to the heat transfer chamber to receive the superheated vaporized working fluid from the heat transfer chamber. The waste heat conversion system may be configured to expand the superheated working fluid through an expander for the conversion of waste heat energy into useful energy. For applications involving internal combustion engines, the system may be configured such that the conversion of waste heat energy into useful energy may drive an air compressor to enhance combustion engine performance and decrease pollutant emissions.

A sensor mounting table for mounting sensors to an aftertreatment system may include a sensor mounting plate having a substantially flat mounting surface for mounting one or more sensors associated with the aftertreatment system. The substantially flat mounting surface may be offset from a heat shield of the aftertreatment system. The sensor mounting table may further include an insulative material disposed between at least a portion of the substantially flat mounting surface of the sensor mounting plate and the heat shield. The sensor mounting plate may be configured to be attached to the aftertreatment system to secure the insulative material between the substantially flat mounting surface of the sensor mounting plate and the heat shield.

The heat shield assembly includes an inner-most layer that is directly engagable with an exhaust component and is of a non-ceramic material with a first heat capacity and a first thermal conductivity. The heat shield assembly additionally includes an insulating layer that surrounds the inner-most layer and is of a material that has a second heat capacity which is lower than the first heat capacity and has a second thermal conductivity which is lower than the first thermal conductivity. The direct engagement of the inner-most layer with the exhaust component allows heat to be easily transferred between the exhaust component and the inner-most layer.

The heat exchanger system includes a first heat exchanger assembly including a plurality of airfoil members circumferentially spaced in a flow stream of an annular duct. Each airfoil member including a radially inner end and a radially outer end and a first internal flowpath configured to channel a flow of cooled fluid therethrough. The heat exchanger assembly includes a second heat exchanger assembly including a plurality of fin members extending proximate the flow stream and a second internal flowpath configured to channel a flow of cooled fluid therethrough. The heat exchanger assembly includes a header system including a conduit path configured to couple the first heat exchanger assembly and the second heat exchanger assembly in flow communication. The header system includes an inlet connection configured to receive a flow of hot fluid from thermal loads and an outlet connection configured to direct cooled fluid to thermal loads.

The heat exchanger assembly includes a first internal flow path configured to channel a flow of fluid to be cooled from a first inlet to a first outlet. The heat exchanger assembly also includes a second internal flow path configured to channel a flow of a first coolant from a first inlet to a first outlet. The heat exchanger assembly further includes an external flow path configured to receive a flow of a second coolant proximate a surface of the external flow path.

The invention is in the field of engine building technology and may be used in space technology or aviation. Liquid-propellant rockets with Laval nozzles are well known, and they have the following insufficiencies: (1) high fuel consumption rates, which lead to increased dimensions and engine weight and boosters; (2) a relatively low combustion efficiency, because the low mass of the combustion products are emitted into the environment; (3) the large length of the de Laval nozzles with increased expansion ratios increase the dimensions and the engine weight; (4) use of high temperature rocket propellants—combustion products—in the camera and de Laval nozzle. These insufficiencies suppress using liquid-propellant rockets in space technology. The goal of the invention is decreasing the influence of these insufficiencies and obtaining an engine with improved efficiency. The goal is achieved with the creation of an engine with the subsonic discharge of combustion products and the creation of a simple nozzle construction.

A thermal heat storage system is provided, including a storage tank, a heat injection system and a heat recovery system. The storage tank holds a material for thermal storage. The heat injection system is coupled to an intake on the storage tank. The heat recovery system is coupled to an output on the storage tank and also uses vapor under depressurized conditions for heat transfer.