A total of 7,740 dedicated COVID-19 facilities in 483 districts have been identified, the Union health ministry said on Sunday, asserting that adequate health infrastructure is in place to fight the pandemic.

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ICICI Bank stock has corrected 37 per cent in three months. But investors should not rush into it

To celebrate their anniversary, the actors went live on their social media channels.

1. NRL 2020: Erin Molan condemns ‘incredibly dangerous’ Holocaust Instagram post, Bryce Cartwright wife  NEWS.com.au
2. Gold Coast Titans anti-vaccination: Doctor’s warning over flu jab after NRL players stood down  Gold Coast Bulletin
3. NRL 2020, news: Bryce Cartwright’s wife hits out in flu shot storm, Instagram post, anti-vaccination players react  NEWS.com.au
4. NRL: Anti-vaxxers stood down as vaccination debate rages on  news.com.au
5. 'It's a team sport boys': Cronulla skipper Wade Graham calls out anti-vaxxers  Wide World of Sports
6. View Full coverage on Google News

Medical officer advises older people to remain cautious as restrictions are eased  Ballarat Courier

1. Medium-intensity earthquake strikes Delhi, adjoining areas; 3rd amid Covid-19 lockdown  Hindustan Times
2. Earthquake of magnitude 3.5 hits Delhi-NCR  Times of India
3. Earthquake in Delhi: Tremors felt in national capital region  The Financial Express
4. Magnitude 3.4 Earthquake Strikes Delhi, Epicentre Near UP Border  News18
5. Breaking news live : Eathquake tremors felt in Delhi, adjoining areas  Times of India
6. View Full coverage on Google News

The following article, NYC Nurse Steals Credit Card From 70-Yr-Old Dying COVID-19 Patient On Ventilator, was first published on 100PercentFedUp.com.

"She is a despicable human being"

Continue reading: NYC Nurse Steals Credit Card From 70-Yr-Old Dying COVID-19 Patient On Ventilator ...

Elizabeth Spiers wrote about an incident where Jared Kushner used the memorial of an employee to congratulate himself for success.

South Dakota Governor Kristi Noem sent letters to two Sioux tribes demanding they remove COVID-19 checkpoints because they interfere with traffic.

In a tweet Saturday morning, Tesla's chief executive said it would file a lawsuit against county officials over not being able to run its factory.

Although a robust inflammatory response is needed to combat infection, this response must ultimately be terminated to prevent chronic inflammation. One mechanism that terminates inflammatory signaling is the production of alternative mRNA splice forms in the Toll-like receptor (TLR) signaling pathway. Whereas most genes in the TLR pathway encode positive mediators of inflammatory signaling, several, including that encoding the MyD88 signaling adaptor, also produce alternative spliced mRNA isoforms that encode dominant-negative inhibitors of the response. Production of these negatively acting alternatively spliced isoforms is induced by stimulation with the TLR4 agonist lipopolysaccharide (LPS); thus, this alternative pre-mRNA splicing represents a negative feedback loop that terminates TLR signaling and prevents chronic inflammation. In the current study, we investigated the mechanisms regulating the LPS-induced alternative pre-mRNA splicing of the MyD88 transcript in murine macrophages. We found that 1) the induction of the alternatively spliced MyD88 form is due to alternative pre-mRNA splicing and not caused by another RNA regulatory mechanism, 2) MyD88 splicing is regulated by both the MyD88- and TRIF-dependent arms of the TLR signaling pathway, 3) MyD88 splicing is regulated by the NF-κB transcription factor, and 4) NF-κB likely regulates MyD88 alternative pre-mRNA splicing per se rather than regulating splicing indirectly by altering MyD88 transcription. We conclude that alternative splicing of MyD88 may provide a sensitive mechanism that ensures robust termination of inflammation for tissue repair and restoration of normal tissue homeostasis once an infection is controlled.

Inter-α-inhibitor is a proteoglycan essential for mammalian reproduction and also plays a less well-characterized role in inflammation. It comprises two homologous “heavy chains” (HC1 and HC2) covalently attached to chondroitin sulfate on the bikunin core protein. Before ovulation, HCs are transferred onto the polysaccharide hyaluronan (HA) to form covalent HC·HA complexes, thereby stabilizing an extracellular matrix around the oocyte required for fertilization. Additionally, such complexes form during inflammatory processes and mediate leukocyte adhesion in the synovial fluids of arthritis patients and protect against sepsis. Here using X-ray crystallography, we show that human HC1 has a structure similar to integrin β-chains, with a von Willebrand factor A domain containing a functional metal ion-dependent adhesion site (MIDAS) and an associated hybrid domain. A comparison of the WT protein and a variant with an impaired MIDAS (but otherwise structurally identical) by small-angle X-ray scattering and analytical ultracentrifugation revealed that HC1 self-associates in a cation-dependent manner, providing a mechanism for HC·HA cross-linking and matrix stabilization. Surprisingly, unlike integrins, HC1 interacted with RGD-containing ligands, such as fibronectin, vitronectin, and the latency-associated peptides of transforming growth factor β, in a MIDAS/cation-independent manner. However, HC1 utilizes its MIDAS motif to bind to and inhibit the cleavage of complement C3, and small-angle X-ray scattering–based modeling indicates that this occurs through the inhibition of the alternative pathway C3 convertase. These findings provide detailed structural and functional insights into HC1 as a regulator of innate immunity and further elucidate the role of HC·HA complexes in inflammation and ovulation.

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

Contact between inflammatory cells and endothelial cells (ECs) is a crucial step in vascular inflammation. Recently, we demonstrated that the cell-surface level of endomucin (EMCN), a heavily O-glycosylated single-transmembrane sialomucin, interferes with the interactions between inflammatory cells and ECs. We have also shown that, in response to an inflammatory stimulus, EMCN is cleared from the cell surface by an unknown mechanism. In this study, using adenovirus-mediated overexpression of a tagged EMCN in human umbilical vein ECs, we found that treatment with tumor necrosis factor α (TNF-α) or the strong oxidant pervanadate leads to loss of cell-surface EMCN and increases the levels of the C-terminal fragment of EMCN 3- to 4-fold. Furthermore, treatment with the broad-spectrum matrix metalloproteinase inhibitor batimastat (BB94) or inhibition of ADAM metallopeptidase domain 10 (ADAM10) and ADAM17 with two small-molecule inhibitors, GW280264X and GI254023X, or with siRNA significantly reduced basal and TNFα-induced cell-surface EMCN cleavage. Release of the C-terminal fragment of EMCN by TNF-α treatment was blocked by chemical inhibition of ADAM10 alone or in combination with ADAM17. These results indicate that cell-surface EMCN undergoes constitutive cleavage and that TNF-α treatment dramatically increases this cleavage, which is mediated predominantly by ADAM10 and ADAM17. As endothelial cell-surface EMCN attenuates leukocyte–EC interactions during inflammation, we propose that EMCN is a potential therapeutic target to manage vascular inflammation.

Written from, different angles and spaces, the poems below testify to the great force of motherhood, which nurtures, strengthens, and manifests love. Happy Mother’s Day – Ann-Margaret Lim Words My mother loved words. Not necessarily in...

Using a nationally representative U.S. birth-cohort study, this report examines levels of school readiness among young children by race/ethnicity and nativity. The authors identify the contextual factors — such as family circumstances, parenting practices, and enrollment in center-based child care — that encourage early school success.

Billy Crystal, Tiffany Haddish, Kenan Thompson and Byron Allen are set to co-host Sunday's Feeding America Comedy Festival.

Testimony of Marc Rosenblum, MPI Senior Policy Analyst, before the National Commission on Fiscal Responsibility and Reform.

With more and more Bollywood celebs taking to social media to interact with their fans amidst the lockdown phase, Kangana Ranaut to Instagram to pour her heart out with a special message which she dedicated to her mother.

Realme Narzo 10 confirmed to feature MediaTek Helio G80 gaming processor  Hindustan TimesRealme Narzo 10 officially confirmed to pack Helio G80 SoC - GSMArena.com news  GSMArena.comRealme Narzo 10, Realme Narzo 10A Launch on May 11:...

Around 340 people participated in the Transform 2013 mission conference in Rome before spreading across the Mediterranean on 29 outreaches.

The students of OM Russia's Discipleship Centre practice sharing their faith with those from a Muslim background.

OM Philippines partners with the local church to provide water and food in areas hit by the 7.2 magnitude earthquake on 15 October.

People who dig through landfills during the week to provide for their families find rest along with spiritual and physical nourishment at church on Sundays.

In postmodern Finland, an international church in Helsinki preaches God’s Word as it is and challenges its congregation to ‘get into the Bible’ daily.

In the spring of 2011, a team from OM Czech Republic trekked in a remote area of the Himalayas on a four-week medical mission.

OM Czech Republic engaged in a medical mission outreach and evangelism ministry to villages in northern Ghana.

God heals physical and spiritual lives during OM Ecuador’s 2014 Medical Brigade in the Saraguro Canton region of Ecuador.

Milena found mission opportunities on OM Germany’s website, which led her to share the love of God in Belgium.

Australia lifted the title after defeating India by 85 runs in the final of the Women's T20 World Cup in Melbourne.

Hero MotoCorp ambulance comes with sleeping arrangements with all the essential equipment. Read along to find more details.

The limited-edition Harley-Davidson Street 750 was launched in India in August 2019 and was costlier than the standard BS4 model by Rs 13,000.

Share Market News Today | Sensex, Nifty, Share Prices Highlights: As many as 28 stocks out of 30 Sensex stocks settled in a deep sea of red with ICICI Bank as top laggard, down 11 per cent, followed by Bajaj Finance, HDFC, IndusInd Bank and Axis Bank.