si The Maestro Project: A Patient Navigator for the Transition of Care for Youth With Type 1 Diabetes By spectrum.diabetesjournals.org Published On :: 2011-02-01 Norma Van WalleghemFeb 1, 2011; 24:9-13From Research to Practice/Transitions in Young Adults with Type 1 Diabetes Full Article
si Iatrogenic Inpatient Hypoglycemia: Risk Factors, Treatment, and Prevention: Analysis of Current Practice at an Academic Medical Center With Implications for Improvement Efforts By spectrum.diabetesjournals.org Published On :: 2008-10-01 Gregory A. MaynardOct 1, 2008; 21:241-247Articles Full Article
si A Model of Community-Based Behavioral Intervention for Depression in Diabetes: Program ACTIVE By spectrum.diabetesjournals.org Published On :: 2010-01-01 Mary de GrootJan 1, 2010; 23:18-25From Research to Practice Full Article
si Nutritional Management of Gastroparesis in People With Diabetes By spectrum.diabetesjournals.org Published On :: 2007-10-01 Carol Rees ParrishOct 1, 2007; 20:231-234Nutrition FYI Full Article
si Integrating Depression Care With Diabetes Care in Real-World Settings: Lessons From the Robert Wood Johnson Foundation Diabetes Initiative By spectrum.diabetesjournals.org Published On :: 2007-01-01 Daren AndersonJan 1, 2007; 20:10-16Feature Articles Full Article
si Transitions in Care from the Hospital to Home for Patients With Diabetes By spectrum.diabetesjournals.org Published On :: 2014-08-01 Karen B. HirschmanAug 1, 2014; 27:192-195From Research to Practice Full Article
si Depressive Affect Among Four Ethnic Groups of Male Patients With Type 2 Diabetes By spectrum.diabetesjournals.org Published On :: 2004-10-01 Lawrence FisherOct 1, 2004; 17:215-219Articles Full Article
si Diabetic Ketoacidosis and Hyperglycemic Hyperosmolar Syndrome By spectrum.diabetesjournals.org Published On :: 2002-01-01 Guillermo E. UmpierrezJan 1, 2002; 15:Articles Full Article
si Redesign of a Diabetes System of Care Using an All-or-None Diabetes Bundle to Build Teamwork and Improve Intermediate Outcomes By spectrum.diabetesjournals.org Published On :: 2010-07-01 Frederick J. BloomJul 1, 2010; 23:165-169From Research to Practice Full Article
si Improving Diabetes Care in the Hospital Using Guideline-Directed Orders By spectrum.diabetesjournals.org Published On :: 2001-10-01 Stephen F. QuevedoOct 1, 2001; 14:Feature Articles Full Article
si Insulin-Related Knowledge Among Health Care Professionals in Internal Medicine By spectrum.diabetesjournals.org Published On :: 2007-07-01 Rachel L. DerrJul 1, 2007; 20:177-185Feature Articles Full Article
si Making a Difference With Interactive Technology: Considerations in Using and Evaluating Computerized Aids for Diabetes Self-Management Education By spectrum.diabetesjournals.org Published On :: 2001-04-01 Russell E. GlasgowApr 1, 2001; 14:Feature Articles Full Article
si Associations Between Self-Management Education and Comprehensive Diabetes Clinical Care By spectrum.diabetesjournals.org Published On :: 2010-01-01 Tammie M. JohnsonJan 1, 2010; 23:41-46Feature Articles Full Article
si Interview-Based Customer Insights in Developing Countries By www8.gsb.columbia.edu Published On :: Wed, 08 Jan 2020 17:00:28 +0000 What are the opportunities and challenges of collecting consumer insights in developing countries—and how can the challenges be overcome? Full Article
si Impossible Foods: Fighting Climate Change with Plant-Based Meat By www8.gsb.columbia.edu Published On :: Thu, 05 Mar 2020 19:24:22 +0000 Can Impossible Foods change the way meat is produced on a large enough scale to make a serious inroad in the battle against climate change? Full Article
si Kaay Jones: The Jamaican who joins region in singing ‘We Got This’ By jamaica-star.com Published On :: Wed, 06 May 2020 05:01:07 -0500 Up-and-coming recording artiste Kaay Jones says she did not know she was becoming part of a transformational music project that would connect her to a wide cross section of talent in the Caribbean. Jones carries Jamaica's flag in a unified... Full Article
si Violence is not the answer - Dexta Daps’ new single spurs conversation on domestic abuse By jamaica-star.com Published On :: Wed, 06 May 2020 05:01:37 -0500 Hours after he was released from police lock-up last week, dancehall artiste Dexta Daps dropped some new music on his eager fans. The track, Breaking News, explores an all-too-familiar domestic violence storyline, but incorporates a controversial... Full Article
si Shav-A sees positives despite COVID By jamaica-star.com Published On :: Thu, 07 May 2020 05:01:16 -0500 Up-and-coming entertainer Shav-A says the COVID-19 pandemic made her reflect on her life. "I needed a reset. With all this downtime, I have realised that I needed to stop and rethink a lot of things that are going on in my life, and it also... Full Article
si What about entertainment? - Industry insider feels sector under-represented in COVID recovery task force By jamaica-star.com Published On :: Fri, 08 May 2020 05:01:40 -0500 Last month, Prime Minister Andrew Holness announced the establishment of an Economic Recovery Task Force, chaired by Finance Minister Dr Nigel Clarke. The multisectoral task force, which is mandated to oversee Jamaica's economic recovery from... Full Article
si It’s becoming depressing - Mother enduring long wait for newborn’s coronavirus results By jamaica-star.com Published On :: Thu, 07 May 2020 05:01:40 -0500 A Manchester mother is pleading with the authorities to provide her with the results of COVID-19 tests done on her and her week-old baby. The woman claims that she has been in isolation in hospital since April 27, a day after she gave birth to... Full Article
si Small business owner looks for silver lining By jamaica-star.com Published On :: Fri, 08 May 2020 05:01:52 -0500 Deborah Fearon is a bar owner who also has a small chicken business. She depends on both for a living. Before the COVID-19 outbreak she was doing well, and had plans of completing her house this year. However, she has been hit hard by the economic... Full Article
si MoBay residents flock COVID ‘giveaway truck’ By jamaica-star.com Published On :: Fri, 08 May 2020 05:02:14 -0500 Despite being placed under strict restrictions and COVID-19 curfew measures, shoppers, commuters, motorists, and vendors in Montego Bay got a feel of Christmas on Wednesday, when they were showered with items as part of the COVID-19 giveaway by an... Full Article
si Leading Through a Protracted Crisis: How to Drive, Survive, and Thrive in a Crisis By www8.gsb.columbia.edu Published On :: Fri, 01 May 2020 00:00:00 -0400 Management professors Adam Galinksy and Paul Ingram, together with Jonathan Laor ’21, CEO of Applicaster, advise on leadership during a crisis. Full Article
si Quick Earthquake Messages M6.7 [7.0S, 130.0E] in Tanimbar Islands Region, Indonesia (21:54 HKT 06/05/2020) By openstreetmap.org Published On :: Wed, 06 May 2020 22:01:16 +0800 Earthquake: 2020-05-06 21:54HKT M6.7 [7.0S, 130.0E] in Tanimbar Islands Region, Indonesia http://openstreetmap.org/?mlat=-7&mlon=130. Full Article
si NYC Silkscreen Studio Swaps Fine Art Prints for Safety Signage By www8.gsb.columbia.edu Published On :: Wed, 22 Apr 2020 00:00:00 -0400 Gary Lichtenstein Editions has partnered with Urban Pathways to increase awareness and safety in the homeless community. Full Article
si Fashion Brands Repurpose Resources to Offer Aid in the COVID-19 Crisis By www8.gsb.columbia.edu Published On :: Wed, 22 Apr 2020 00:00:00 -0400 Retail giants like Yoox Net-a-Porter Group and Brooks Brothers quickly pivoted to offer life-saving services. Full Article
si Columbia-Harlem Small Business Development Center Is a Lifeline for Business Owners By www8.gsb.columbia.edu Published On :: Tue, 28 Apr 2020 00:00:00 -0400 The SBDC offers resources and guidance to Harlem’s small businesses amidst the COVID-19 crisis. Full Article
si Recommitting to International Criminal Justice and Human Rights in Indonesia By feedproxy.google.com Published On :: Fri, 06 Apr 2018 15:19:11 +0000 6 April 2018 Agantaranansa Juanda Academy Associate, International Law Programme LinkedIn Jason Naselli Senior Digital Editor LinkedIn Agantaranansa Juanda speaks to Jason Naselli about the promises the government has made and the steps that still need to be taken for the country to deliver justice for past violations of human rights. 2018-04-06-Jokowi.jpg Indonesian PM Joko Widodo. Photo: Getty Images. Does the Indonesian government adequately protect human rights?It does and it does not; it really depends on the context. Indonesia looks good among its neighbours in Southeast Asia in terms of protection of civil and political rights, and to some extent economic, social and cultural rights, although room for improvements exists.But one of the promises of the current president, Joko Widodo, during his 2014 campaign was about international criminal justice, which involves rights for many victims of past cases of human rights abuses in Indonesia. In that sense, it does not protect these rights, including the rights to justice, truth, reparations or guarantees of non-recurrence.For example, in the case of the conflict over independence for East Timor in 1999, there were many gross violations of human rights. However, there has never been any sort of effective judicial process to address gross violations of human rights, and crimes against humanity in particular.In 1965–66, during the government’s violent anti-communist operations, 500,000 people or more were killed. Indonesia’s National Commission on Human Rights was tasked with conducting an investigation into this period within its limited mandate, but it led to nothing; there have never been any prosecutions relating to these crimes.The election promise of the current president was to deal with a number of these past human rights cases, and this promise has not been met at all. His opponent in 2014, Prabowo Subianto, was a former military general involved in alleged past human rights abuses, so it was politically expedient to make such a promise. But it has not been pursued in office.In 2000, Indonesia established its own Human Rights Court. What is your assessment of its record?Some human rights activists suggested that the establishment of the Human Rights Court took place under international pressure following the independence of East Timor. To avoid international scrutiny, for example the creation of an ad hoc international tribunal, the government established this court.Based on the report of the International Commission of Inquiry on East Timor in 2000, it was indeed recommended that an international human rights tribunal be set up. Indonesian government rejected the proposal with strong assurances that it would provide justice for atrocities committed by its nationals. So it is fair for some to see the establishment of Indonesia’s Human Rights Court as a political move by the government at that time, in order to avoid scrutiny by the international community.When it comes to performance, the Human Rights Court actually investigated and prosecuted cases relating to atrocities in East Timor. There were around 100 suspects identified, and 18 were put on trial. Out of these 18, only one trial, of Eurico Guterres, ended in a conviction for crimes against humanity. However, the Indonesian Supreme Court cleared Guterres of all charges in 2008. So the Human Rights Court did take steps, but the net result amounted to essentially nothing. Impunity remains.So it has not lived up to its mandate, but there is another factor, which is that the founding law of the Human Rights Court does not accommodate international standards of criminal justice. It only covers two of the four categories of crime as outlined in the Rome Statute – crimes against humanity and genocide. It also does not provide adequate protection for victims and witnesses. So there are issues not only with the performance of the Human Rights Court but also with the legislation establishing it.Why hasn’t Indonesia become a party to the Rome Statute to join the ICC?The main opposition came from the military, because they were afraid of being targeted by the ICC. There was also a lot of discussion about Indonesia’s ‘sovereign right to prosecute’.But what those opposing failed to understand is that the ICC is bound by temporal and territorial boundaries, meaning that it will not intervene if the state in question is able and willing to prosecute. So I think accession to the Rome Statute has not taken place because of this misunderstanding.I think another factor since this was initially raised is there is a focus on other issues. Indonesia is an emerging country economically; there is a focus on building infrastructure. So many in government feel like they are done with the past. But for the millions of victims of past crimes and their families, the past is not done.So it’s very important at this point in the country’s history to revisit the commitment to international criminal justice to be able to contribute to sustainable peace and development.What steps could the Indonesian government take to improve how it handles these issues?The establishment of the Human Rights Court was an important starting point, but clearly there has to be significant reform, both in terms of the substantive law underpinning it and its procedures.Clearly the domestic laws need to be reformed, but also, an effort needs to be made to improve the courts capacity in terms of manpower and logistical support. This is why the government needs to restart the discussion about becoming a party to the Rome Statute. Through the outreach programme of the ICC, this would give the Human Rights Court the capacity, in terms of manpower and logistical support, to tackle past human rights violations in Indonesia, which the Human Rights Court is currently lacking.Only if these two steps are taken – reforming the domestic Human Rights Court and restarting discussion about becoming a party to the Rome Statute – will the Indonesian government be able to say it has made progress on international criminal justice.The Indonesian government is actually running for a seat on the UN Security Council for the period of 2019–20. So I think it is an urgent discussion that the Indonesian government needs to have before it makes another pledge to contribute to the maintenance of international peace and security. It is difficult to have sustainable peace without justice. Full Article
si Responsible Business 2019 By feedproxy.google.com Published On :: Tue, 04 Sep 2018 08:40:01 +0000 Conference Priorities, practices and principles in a digital age 28 February 2019 - 9:30am to 5:00pm Chatham House, London Book now Book now Book now Overview Agenda Speakers Pricing and booking information Sponsors Media partners and supporting organizations Venue and accommodation Press registration Contact us Ever-increasing consumer, investor and employee awareness continues to drive the business case for responsible behaviour, and recent events have highlighted the growing need for corporate accountability and transparency from C-suite behaviour to global supply chain management. But what exactly are these expectations across different sectors, as the acceleration of the digital age continues to present new risks, opportunities and concerns? How can the right behaviours be encouraged? Furthermore, ongoing political transitions and regulatory stances over the last two years have shone a light on companies’ potential and realized impact on society. With trust in political institutions low, many are calling upon businesses not only to reflect their values but to actively bridge the governance gap on issues such as equality, sustainability and human rights, in their own business operations and beyond. But what role should business be adopting, and what are the consequences of this trend? What are the perceived trade-offs? The past year has seen examples of technology leaders being held to account for the mishandling of data, global corporations taking a proactive stance on contentious political issues and executive behaviour directly impacting share price. It is critical that policy-makers and business leaders re-evaluate their priorities, practices and principles as technology and politics continue to reshape the landscape. The third annual Chatham House Responsible Business conference will explore key questions, including:Who will lead the corporate responsibility agenda? What is driving responsible behaviour? To what extent has there been a policy retreat in this space? What has been the response to this, and what does it mean for different roles and responsibilities? What are the new priorities for responsible business in a digital age? How have recent events demonstrated a shift in expectations? How can policy-makers and companies foster the integration of human rights across global supply chains, international trade and regional business operations?What is the role of corporate governance and leadership in setting standards and promoting responsible business? Is this a new era of corporate activism? The Chatham House RuleTo enable as open a debate as possible, this conference will be held under the Chatham House Rule.Twitter@CH_Events#CHBusiness Thursday 28 February0920Welcome and chair's opening remarksDr Robin Niblett, Director, Chatham HouseKeynote addressSimon Thompson, Chairman, Rio TintoQuestions and discussionSession One | Leaders and Drivers1000-1115This opening session will examine the status of corporate leadership in responsible business, evolving policy environments and stakeholder expectations, and how they continue to shape roles and responsibilities. How are expectations for responsible business evolving? Where has this been most apparent in the past 18 months, and what is driving these shifts? Do policy and regulation continue to be effective at encouraging responsible business behaviour? Where is regulation most effective in this space? Given current political sentiments and levels of trust, what role should business play in creating a sustainable economy and a more equal society? What are the implications of this? What can business leaders learn from existing examples of corporate activism? Does a trade-off exist between responsible practices and economic competitiveness, or do commercial drivers incentivize better behaviours? How does this differ across different markets and regions?ChairTamzin Booth, European Business Editor, The EconomistSpeakersHelena Morrissey, Head of Personal Investing, Legal & General Investment Management, and Founder of the 30% ClubIoannis Ioannou, Associate Professor of Strategy and Entrepreneurship, London Business SchoolPhil Bloomer, Executive Director, Business & Human Rights Resource CentreSue Garrard, EVP Sustainable Business and Communications, Unilever (2014-18)Questions and discussion1115-1145 RefreshmentsSession Two | Practices and Transparency1145-1300This session will address regulatory frameworks and economic incentives governing responsible business conduct, as well as human rights and business operations across global supply chains. How prominent are business and human rights issues on national policy agendas? To what extent has this influenced business behaviours across different sectors and regions? What progress has been made with regards to the UN Guiding Principles for Business and Human Rights? What has most effectively driven their integration? To what extent can public opinion and public awareness help narrow the governance gap? How can investors actively promote and enforce better governance practices? Does transparency necessarily lead to accountability, and in turn increase consumer and investor trust? What are the implications of this? How can due diligence and reporting be made more efficient to enhance accountability as well as transparency across supply chains and investments? Can digital solutions be employed to achieve social and environmental best practice?ChairBennett Freeman, Deputy Assistant Secretary for Democracy, Human Rights and Labor, US Department of State (1999–2001)SpeakersGillian Caldwell, CEO, Global WitnessMadelaine Tuininga, Head of Unit, DG Trade, European CommissionSharan Burrow, General Secretary, International Trade Union Confederation Guus Houttuin, Trade Adviser, European External Action Service, and Chair, the OECD Multi-stakeholder Steering Group Questions and discussion1300-1400 LunchSession Three | Responsibility in the Digital Age1400-1515This session will examine the risks and opportunities presented by digitization and other technological developments for responsible business practices. What have events over the past year indicated regarding stakeholder concerns accompanying specific technologies and business practices? How have different sectors and companies responded? What are the responsible business issues that accompany the transition to a digital-first society? What can be learned from the challenges of regulating tech titans?How can businesses ensure data privacy and help customers with digital access to maintain trust and maximize benefits from digital transformation? To what extent is the proliferation of information through technology already enhancing corporate accountability and transparency? What are the risks here? ChairJohn Thornhill, Innovation Editor, Financial TimesSpeakersNuala O’Connor, President and CEO of the Center for Democracy and TechnologySimon McDougall, Executive Director, Technology Policy and Innovation, Information Commissioner’s OfficeRebecca MacKinnon, Director, Ranking Digital Rights, New AmericaSarah Drinkwater, Director, Tech and Society Solutions Lab, Omidyar NetworkQuestions and discussion1515-1545 Afternoon refreshmentsSession Four | Governance1545-1700This closing session will explore the role of corporate governance in setting standards and leading responsible business practices, including diversity and inclusion as well as C-suite accountability and transparency. How are businesses responding to expectations of good corporate governance and leadership in the digital age? Are businesses equipped to maintain high standards of accountability and transparency in the era of open data and social media?Do business leaders have a responsibility to embody as well as promote high standards of responsible business and ethical leadership? What lessons can be learnt from corporate governance failings? What role should business leaders play in the broader political environment? Is corporate activism part of responsible governance, and what are the risks?What are the best strategies to empower diversity and foster inclusion in a rapidly changing global economy? Should diversity and inclusion begin in the boardroom?ChairAris Vrettos, Director of Open Programmes and International Markets, Cambridge Institute for Sustainability LeadershipSpeakersCatherine Howarth, CEO, ShareAction Jane Ellis, Director, GoodCorporationMo Ibrahim, Founder, Mo Ibrahim Foundation Alison Cottrell, CEO, Banking Standards BoardQuestions and discussion1700 Close of conference and drinks reception © The Royal Institute of International Affairs 2019 Keynote Speaker Simon Thompson Chairman, Rio Tinto Speakers Phil Bloomer Executive Director, Business & Human Rights Resource Centre Tamzin Booth Business Editor, The Economist Sharan Burrow General Secretary, International Trade Union Confederation Gillian Caldwell CEO, Global Witness Alison Cottrell CEO, Banking Standards Board Sarah Drinkwater Director, Tech and Society Solutions Lab, Omidyar Network Jane Ellis Director, GoodCorporation Bennett Freeman Deputy Assistant Secretary for Democracy, Human Rights and Labor, United States (1999-2001) Sue Garrard EVP Sustainable Business and Communications, Unilever (2014-18) Guus Houttuin Trade Adviser, European External Action Service, and Chair, the OECD Multi-stakeholder Steering Group Catherine Howarth CEO, ShareAction Mo Ibrahim Founder, Mo Ibrahim Foundation Ioannis Ioannou Associate Professor of Strategy and Entrepreneurship, London Business School Rebecca MacKinnon Director, Ranking Digital Rights, New America Simon McDougall Executive Director, Technology Policy and Innovation, Information Commissioner's Office Dame Helena Morrissey Head of Personal Investing, Legal & General Investment Management; Founder, 30% Club Dr Robin Niblett CMG Director, Chatham House Nuala O'Connor President and CEO, Center for Democracy & Technology John Thornhill Innovation Editor, Financial Times Madelaine Tuininga Head of Unit, DG Trade, European Commission Aris Vrettos Director of Open Programmes and International Markets, Cambridge Institute for Sustainability Leadership Ways to book:Online: Click here to complete the online registration formPhone: Call Boudicca Georgii Hellberg on +44 (0) 20 7314 2785Email/Post: Download a PDF registration form, complete and return to Boudicca Georgii Hellberg via email or post: Chatham House, 10 St. James Square, London, SW1Y 4LECheck if your organization is a member of Chatham House here. RATE (+VAT):Partners and major corporate members All organizations£595Standard corporate members Commercial organizations£1,180Government departments/agencies/intergovernmental organizations£700NGOs/academic institutions/associations (including not for profits and registered charities)£460Non-members Commercial organizations£1,295Government departments/agencies/intergovernmental organizations£750NGOs/academic insitutions/associations (including not for profits and registered charities)£510 Your delegate pass includes:DocumentationLunch and refreshmentsTravel and accommodation are not included. If you are interested in becoming a sponsor for this event, please contact Olivia Lewis on +44 (0)20 7957 5732 If you are interested in partnering with Chatham House on this event, please contact Ayesha Arif on +44 (0)20 7957 5753 Chatham House10 St James's SquareLondonSW1Y 4LEUKconferences@chathamhouse.orgTelephone: +44 (0)20 7957 5643Fax: +44 (0)20 7957 5710If you wish to book the venue for your own event please phone +44 (0)20 7314 2764DirectionsThe nearest tube station is Piccadilly Circus which is on the Piccadilly and the Bakerloo Underground lines. From Piccadilly follow Regent Street southwards towards Pall Mall and take the first road on the right called Jermyn Street. Duke of York Street is the second road on the left and leads to St James's Square. Chatham House is immediately on your right.MapAccommodationAlthough we cannot book accommodation for delegates, we have arranged a reduced rate at some nearby hotels, where you can book your own accommodation. Please inform the hotel that you will be attending a conference at Chatham House (The Royal Institute of International Affairs) to qualify for the Institute's reduced rate.Please note all rates are subject to availability.Flemings Mayfair13 Half Moon StreetMayfairLondon - W1J 7BHTel: + 44 (0)20 7499 2964Fax: + 44 (0)20 7499 1817reservations@flemings.co.ukClassic Double without breakfast: £195 +VATThe Cavendish London81 Jermyn StreetLondon - SW1U 6JFTel: + 44 (0)20 7930 2111Fax: + 44 (0)20 7839 2125enquiry.cavendish@the-ascott.com Classic Room without breakfast: £195 +VATBook The Cavendish onlineThe Stafford London St James's PlaceLondon - SW1A 1NJTel: 020 7493 0111Fax: 020 7493 7121reservations@thestaffordlondon.comClassic Queen without breakfast: £247 +VATQuote Chatham House This conference will be held under the Chatham House Rule. Information for journalists.Press can request a press pass. For enquiries relating to the conference agenda or sponsorship please call Olivia Lewis on +44 (0) 20 7957 5732For registration enquiries please call Boudicca Georgii Hellberg on +44 (0) 20 7314 2785For general enquiries please email conferences@chathamhouse.org Department/project International Law Programme Full Article
si Geneva Launch: Protecting Civilians — When is ‘Incidental Harm’ Excessive? By feedproxy.google.com Published On :: Tue, 02 Oct 2018 13:45:01 +0000 Research Event 14 December 2018 - 10:00am to 11:30am Graduate Institute | Chemin Eugène-Rigot 2 | 1202 Geneva | Switzerland Event participants Emanuela-Chiara Gillard, Associate Fellow, International Law Programme, Chatham HouseEzequiel Heffes, Thematic Legal Adviser, Geneva CallSigrid Redse Johansen, Judge Advocate General, The Norwegian Armed ForcesChair: Elizabeth Wilmshurst, Distinguished Fellow, Chatham HouseFurther speakers to be announced. PLEASE NOTE THIS EVENT IS BEING HELD IN GENEVA.There have been large numbers of civilian deaths in the armed conflicts in Yemen and Syria. Is international humanitarian law being ignored? This meeting coincides with the launch of a Chatham House research paper on the incidental harm side of the proportionality assessment which belligerents are legally required to make. The panel at the meeting will consider the types of harm that fall within the scope of proportionality assessments, what constitutes ‘excessive’ harm and measures that belligerents can take to give effect to the rule on proportionality.This event will be followed by a reception. Department/project International Law Programme Chanu Peiris Programme Manager, International Law +44 (0)20 7314 3686 Email Full Article
si London Launch: Protecting Civilians — When is ‘Incidental Harm’ Excessive? By feedproxy.google.com Published On :: Tue, 06 Nov 2018 18:25:01 +0000 Research Event 14 January 2019 - 5:30pm to 7:00pm Chatham House, London Event participants Emanuela-Chiara Gillard, Associate Fellow, International Law Programme, Chatham HouseEzequiel Heffes, Thematic Legal Adviser, Geneva CallSigrid Redse Johansen, Judge Advocate General, Norwegian Armed ForcesAndrew Murdoch, Legal Director, UK Foreign & Commonwealth OfficeChair: Elizabeth Wilmshurst, Distinguished Fellow, International Law Programme, Chatham House There have been large numbers of civilian deaths in the armed conflicts in Yemen and Syria. Is international humanitarian law being ignored?This meeting marks the London launch of a Chatham House research paper on the incidental harm side of the proportionality assessment which belligerents are legally required to make. The panel at the meeting will consider the types of harm that fall within the scope of proportionality assessments, what constitutes ‘excessive’ harm and measures that belligerents can take to give effect to the rule on proportionality.This event will be followed by a reception. Department/project International Law Programme, Rights, Accountability and Justice Chanu Peiris Programme Manager, International Law +44 (0)20 7314 3686 Email Full Article
si Proportionality in the Conduct of Hostilities: The Incidental Harm Side of the Assessment By feedproxy.google.com Published On :: Thu, 06 Dec 2018 14:56:29 +0000 10 December 2018 Clarification of international humanitarian law is important in ensuring compliance with the rule of proportionality, but a culture of compliance within armed forces and groups is also crucial. Download PDF Emanuela-Chiara Gillard Associate Fellow, International Law Programme 2018-12-10-ilp-proportionality-paper.jpg Members of civil right defence conduct a search and rescue operation on destroyed buildings after an airstrike was carried out over the city of Jisr al-Shughur in Idlib province in Syria, on 6 May 2018. Photo: Hadi Harrat/Anadolu Agency/Getty Images. SummaryMilitary operations are taking place with increasing frequency in densely populated areas. Such operations result in loss of life and harm to civilians, as well as damage to civilian objects, (including infrastructure providing essential services). In order to protect civilians, it is imperative that armed forces and groups comply with the rules of international humanitarian law on the conduct of hostilities, including the rule of proportionality.The rule of proportionality prohibits attacks which may be expected to cause incidental loss of civilian life, injury to civilians, damage to civilian objects or a combination thereof, which would be excessive in relation to the concrete and direct military advantage anticipated. This research paper analyses the key steps that belligerents must take to give effect to the rule, with a particular focus on one side of proportionality assessments – the expected incidental harm.Those undertaking proportionality assessments before or during an attack must consider whether the expected harm will be caused by the attack, and whether that harm could be expected (that is, was it reasonably foreseeable).For the purpose of proportionality assessments, injury to civilians includes disease, and there is no reason in principle to exclude mental harm, even though it is currently challenging to identify and quantify it. Damage to civilian objects includes damage to elements of the natural environment.Once the incidental harm to be considered has been identified, a value or weight must be assigned to it. This is then balanced against the value or weight of the military advantage anticipated from the attack to determine whether the harm would be excessive.In the determination of whether the expected incidental harm would be excessive compared to the anticipated military advantage, ‘excessive’ is a wide but not indeterminate standard.Belligerents should develop methodologies so that those planning and deciding attacks are provided with all necessary information on expected incidental harm, and to assist them in assigning weight to the incidental harm to be considered.If it becomes apparent that the rule of proportionality will be contravened, the attack in question must be cancelled or suspended.Clarification of the law is important in ensuring compliance with the rule of proportionality, but a culture of compliance within armed forces and groups, inculcated by their leaders, is also crucial. Department/project International Law Programme, The Limits on War and Preserving the Peace Full Article
si Security and Prosperity in the Asia-Pacific: The Role of International Law By feedproxy.google.com Published On :: Thu, 17 Jan 2019 11:25:01 +0000 Research Event 27 March 2019 - 10:00am to 5:00pm Chatham House | 10 St James's Square | London | SW1Y 4LE Agendapdf | 119.32 KB Security and Prosperity in Asia: The Role of International Lawpdf | 881.12 KB Event participants Koji Tsuruoka, Ambassador of Japan to the United KingdomBen Saul, Associate Fellow, International Law Programme, Chatham House; Challis Chair of International Law, Australian National UniversityLee Chen Chen, Director, Singapore Institute of International AffairsAniruddha Rajput, Member, UN International Law Commission; Consultant, Withersworldwide The rapid growth in the Asia-Pacific’s economic and political power has significant implications for global governance. Asia-Pacific countries such as Japan, India and China – and regional bodies such as ASEAN – are increasingly informing, influencing and seeking to shape international standards and norms.This conference will bring together international law and policy experts to explore the political and legal dynamics affecting economic relations, security challenges and maritime governance in the region.Given security and prosperity challenges within the region as well as the increasingly complex environment for global governance, to what extent is international law operating as a tool of cooperation in the Asia-Pacific? In which areas is it a source of friction?And what are the broader implications for global governance including the development of international law? Department/project International Law Programme, Asia-Pacific Programme, Geopolitics and Governance Chanu Peiris Programme Manager, International Law +44 (0)20 7314 3686 Email Full Article
si Sieges, the Law and Protecting Civilians By feedproxy.google.com Published On :: Thu, 27 Jun 2019 10:35:30 +0000 27 June 2019 Siege warfare has been employed throughout the ages and remains dramatically relevant today. Questions of the compatibility of this practice with international humanitarian law (IHL) arise when besieged areas contain civilians as well as enemy forces. This briefing addresses those rules of IHL that are particularly relevant to sieges. Read online Download PDF Emanuela-Chiara Gillard Associate Fellow, International Law Programme 2019-06-27-Syrian-Family.jpg A Syrian family gather to eat a plate of corn and cabbage in Saqba, in the besieged rebel-held Eastern Ghouta area near Damascus on 6 November 2017. Photo: Getty Images SummaryAlthough sieges may conjure up images of medieval warfare, they are still used by armed forces today, in international and non-international armed conflicts.International law does not define sieges, but their essence is the isolation of enemy forces from reinforcements and supplies. Sieges typically combine two elements: ‘encirclement’ of an area for the purpose of isolating it, and bombardment.Questions of the compatibility of sieges with modern rules of international humanitarian law (IHL) arise when besieged areas contain civilians as well as enemy forces.Sieges are not prohibited as such by either IHL or other areas of public international law.Three sets of rules of IHL are relevant to sieges. The first comprises the rules regulating the conduct of hostilities. The second is the prohibition of starvation of civilians as a method of warfare, along with the rules regulating humanitarian relief operations. The third comprises the rules on evacuation of civilians.The application of IHL to sieges is unsettled in some respects. This briefing does not purport to resolve all the difficulties or address all the issues in detail.While it may go too far to say that it is now impossible to conduct a siege that complies with IHL, the significant vulnerability of civilians caught up in sieges puts particular emphasis on the need for both besieging and besieged forces to comply scrupulously with the legal provisions for the protection of civilians and to conclude agreements for their evacuation. Department/project International Law Programme, The Limits on War and Preserving the Peace Full Article
si Tackling Cyber Disinformation in Elections: Applying International Human Rights Law By feedproxy.google.com Published On :: Wed, 18 Sep 2019 10:30:02 +0000 Research Event Tackling Cyber Disinformation in Elections: Applying International Human Rights Law 6 November 2019 - 5:30pm to 7:00pm Chatham House | 10 St James's Square | London | SW1Y 4LE Susie Alegre, Barrister and Associate Tenant, Doughty Street ChambersEvelyn Aswad, Professor of Law and the Herman G. Kaiser Chair in International Law, University of OklahomaBarbora Bukovská, Senior Director for Law and Policy, Article 19Kate Jones, Director, Diplomatic Studies Programme, University of OxfordChair: Harriet Moynihan, Associate Fellow, International Law Programme, Chatham House Register Interest Cyber operations are increasingly used by political parties, their supporters and foreign states to influence electorates – from algorithms promoting specific messages to micro-targeting based on personal data and the creation of filter bubbles. The risks of digital tools spreading disinformation and polarizing debate, as opposed to deepening democratic engagement, have been highlighted by concerns over cyber interference in the UK’s Brexit referendum, the 2016 US presidential elections and in Ukraine. While some governments are adopting legislation in an attempt to address some of these issues, for example Germany’s ‘NetzDG’ law and France’s ‘Law against the manipulation of information’, other countries have proposed an independent regulator as in the case of the UK’s Online Harms white paper. Meanwhile, the digital platforms, as the curators of content, are under increasing pressure to take their own measures to address data mining and manipulation in the context of elections. How do international human rights standards, for example on freedom of thought, expression and privacy, guide the use of digital technology in the electoral context? What practical steps can governments and technology actors take to ensure policies, laws and practices are in line with these fundamental standards? And with a general election looming in the UK, will these steps come soon enough? This event brings together a wide range of stakeholders including civil society, the tech sector, legal experts and government, coincides with the publication of a Chatham House research paper on disinformation, elections and the human rights framework. Jacqueline Rowe Programme Assistant, International Law Programme 020 7389 3287 Email Department/project International Law Programme, Cyber, Sovereignty and Human Rights, Rights, Accountability and Justice Full Article
si Security and Prosperity in Asia: The Role of International Law By feedproxy.google.com Published On :: Fri, 01 Nov 2019 11:38:35 +0000 1 November 2019 The 'Security and Prosperity in Asia' conference looked at the impact of international law in the Asia-Pacific with a focus on regional economic and security issues such as the South China Sea disputes. Read online Download PDF Security and Prosperity in Asia Cover Image.jpg Singapore skyline at sunset, 2016. Photo: Getty Images. About the ConferenceAt a time of geopolitical uncertainty and with multilateralism under pressure, this conference brought together diverse actors to explore the evolving role of international law on critical security and economic issues in the Asia-Pacific. From trade agreements to deep-sea mining, cyberwarfare to territorial disputes, the breadth of the discussion illustrated the growing reach of international law in the region.Hosted by the International Law Programme and the Asia-Pacific Programme at Chatham House on 27 March 2019, the conference focused on three themes: trade and investment, maritime security and governance, and emerging security challenges. What trends are emerging in terms of engagement with international law in the region, and how can international standards play a greater role in encouraging collaboration and reducing tensions? And, with the eastward shift in geopolitical power, how will Asia-Pacific states shape the future of international law? Department/project International Law Programme, Global Governance and the Rule of Law, Asia-Pacific Programme, Geopolitics and Governance, Trade, Investment and Economics Full Article
si Online Disinformation and Political Discourse: Applying a Human Rights Framework By feedproxy.google.com Published On :: Tue, 05 Nov 2019 11:03:02 +0000 6 November 2019 Although some digital platforms now have an impact on more people’s lives than does any one state authority, the international community has been slow to hold to account these platforms’ activities by reference to human rights law. This paper examines how human rights frameworks should guide digital technology. Download PDF Kate Jones Associate Fellow, International Law Programme @katejones77 LinkedIn 2019-11-05-Disinformation.jpg A man votes in Manhattan, New York City, during the US elections on 8 November 2016. Photo: Getty Images. SummaryOnline political campaigning techniques are distorting our democratic political processes. These techniques include the creation of disinformation and divisive content; exploiting digital platforms’ algorithms, and using bots, cyborgs and fake accounts to distribute this content; maximizing influence through harnessing emotional responses such as anger and disgust; and micro-targeting on the basis of collated personal data and sophisticated psychological profiling techniques. Some state authorities distort political debate by restricting, filtering, shutting down or censoring online networks.Such techniques have outpaced regulatory initiatives and, save in egregious cases such as shutdown of networks, there is no international consensus on how they should be tackled. Digital platforms, driven by their commercial impetus to encourage users to spend as long as possible on them and to attract advertisers, may provide an environment conducive to manipulative techniques.International human rights law, with its careful calibrations designed to protect individuals from abuse of power by authority, provides a normative framework that should underpin responses to online disinformation and distortion of political debate. Contrary to popular view, it does not entail that there should be no control of the online environment; rather, controls should balance the interests at stake appropriately.The rights to freedom of thought and opinion are critical to delimiting the appropriate boundary between legitimate influence and illegitimate manipulation. When digital platforms exploit decision-making biases in prioritizing bad news and divisive, emotion-arousing information, they may be breaching these rights. States and digital platforms should consider structural changes to digital platforms to ensure that methods of online political discourse respect personal agency and prevent the use of sophisticated manipulative techniques.The right to privacy includes a right to choose not to divulge your personal information, and a right to opt out of trading in and profiling on the basis of your personal data. Current practices in collecting, trading and using extensive personal data to ‘micro-target’ voters without their knowledge are not consistent with this right. Significant changes are needed.Data protection laws should be implemented robustly, and should not legitimate extensive harvesting of personal data on the basis of either notional ‘consent’ or the data handler’s commercial interests. The right to privacy should be embedded in technological design (such as by allowing the user to access all information held on them at the click of a button); and political parties should be transparent in their collection and use of personal data, and in their targeting of messages. Arguably, the value of personal data should be shared with the individuals from whom it derives.The rules on the boundaries of permissible content online should be set by states, and should be consistent with the right to freedom of expression. Digital platforms have had to rapidly develop policies on retention or removal of content, but those policies do not necessarily reflect the right to freedom of expression, and platforms are currently not well placed to take account of the public interest. Platforms should be far more transparent in their content regulation policies and decision-making, and should develop frameworks enabling efficient, fair, consistent internal complaints and content monitoring processes. Expertise on international human rights law should be integral to their systems.The right to participate in public affairs and to vote includes the right to engage in public debate. States and digital platforms should ensure an environment in which all can participate in debate online and are not discouraged from standing for election, from participating or from voting by online threats or abuse. Department/project International Law Programme, Cyber, Sovereignty and Human Rights, Rights, Accountability and Justice Full Article
si Power Politics Could Impede Progress on Responsible Regulation of Cyberspace By feedproxy.google.com Published On :: Tue, 03 Dec 2019 14:34:13 +0000 3 December 2019 Harriet Moynihan Senior Research Fellow, International Law Programme @HarrietMoyniha9 A new Chatham House paper examines the prospects of countries reaching agreement on issues of sovereignty and non-intervention in cyberspace in the face of persistent, low-level, state-to-state cyber attacks. 2019-11-29-Intl-Law-Cyberattacks.jpg A computer hacked by a virus known as Petya. The Petya ransomware cyberattack hit computers of Russian and Ukrainian companies on 27 June 2017. Photo: Getty Images. In discussions to date about how international law applies in cyberspace, commentators have tended to focus their attention on how the rules on the use of force, or the law of armed conflict, apply to cyber activities conducted by states that give rise to physical damage, injury or death.But in practice, the vast majority of state cyberattacks fall below this threshold. Far more common are persistent, low-level attacks that may leave no physical trace but that are capable of doing significant damage to a state’s ability to control its systems, often at serious economic cost.Such cyber incursions might include network disruptions in the operation of another government’s websites; tampering with electoral infrastructure to change or undermine the result; or using cyber means to destabilize another state’s financial sector.For these kinds of cyber operation, the principle of sovereignty, and the principle of non-intervention in another state’s internal affairs, are the starting point.A UN Group of Government Experts (GGE) agreed in 2013 and 2015 that the principles in the UN Charter, including sovereignty and the prohibition on intervention in another state’s affairs, apply to states’ activities in cyberspace. The 2015 GGE also recommended eleven (non-binding) norms of responsible state behaviour in cyberspace.However, states have not yet reached agreement on how to apply these principles. Until recently, there has also been very little knowledge of what states actually do in cyberspace, as they usually conduct cyber operations covertly and have been reluctant to put their views on record.A new Chatham House research paper analyses the application of the principles of sovereignty and non-intervention to state cyberattacks that fall below the principle of use of force. As well as analysing the application of the law in this area, the paper also makes recommendations to governments on how they might best make progress in reaching agreement in this area.Existing rules or new rules?As the research paper makes clear, there is currently some debate, principally between countries in the West, about the extent to which sovereignty is a legally binding rule in the context of cyberspace and, if so, how it and the principle of non-intervention might apply in practice.In the last few years, certain states have put on record how they consider international law to apply to states’ activities in cyberspace, namely the UK, Australia, France and the Netherlands. While there may be some differences in their approaches, which are discussed in the paper, there also remains important common ground: namely, that existing international law already provides a solid framework for regulating states’ cyber activities, as it regulates every other domain of state-to-state activity.There is also an emerging trend for states to work together when attributing cyberattacks to hostile states, enabling them to call out malign cyber activity when it violates international law. (See, for example, the joint statements made in relation to the NotPetya cyber attack and malicious cyber activity attributed to the Russian government).However, other countries have questioned whether existing international law as it stands is capable of regulating states’ cyber interactions and have called for ‘new legal instruments’ in this area.This includes a proposal by the Shanghai Cooperation Organization (led by Russia and China) for an International Code of Conduct on Information Security, a draft of which was submitted to the UN in 2011 and 2015, without success. The UN has also formed a new Open-Ended Working Group (OEWG) under a resolution proposed by Russia to consider how international law applies to states’ activities in cyberspace.The resolution establishing the OEWG, which began work earlier this year, includes the possibility of the group ‘introducing changes to the rules, norms and principles of responsible behaviour of States’ agreed in the 2013 and 2015 GGE reports. In the OEWG discussions at the UN in September, several countries claimed that a new legal instrument was needed to fill the ‘legal vacuum’ (Cuba) or ‘the gap of ungoverned areas’ (Indonesia).It would be concerning if the hard-won consensus on the application of international law to cyberspace that has been reached at past GGEs started to unravel. In contrast to 2013 and 2015, the 2017 meeting failed to reach an agreement.On 9 December, a renewed GGE will meet in New York, but the existence of the OEWG exploring the same issues in a separate process reflects the fact that cyber norms have become an area of geopolitical rivalry.Aside from the application of international law, states are also adopting divergent approaches to the domestic regulation of cyberspace within their own territory. The emerging trend towards a ‘splinternet’ – i.e. between states that believe the internet should be global and open on the hand, and those that favour a ‘sovereignty and control’ model on the other – is also likely to make discussions at the GGE more challenging.Distinct from the international law concept of sovereignty is the notion of ‘cybersovereignty’, a term coined by China to describe the wide-ranging powers it assumes under domestic law to regulate its citizens’ access to the internet and personal data within its territory. This approach is catching on (as reflected in Russia’s recently enacted ‘Sovereign Internet Law’), with other authoritarian states likely to follow suit.The importance of non-state actorsIn parallel with regional and UN discussions on how international law applies, a number of initiatives by non-state actors have also sought to establish voluntary principles about responsible state behaviour in cyberspace.The Global Commission on the Stability of Cyberspace, a multi-stakeholder body that has proposed principles, norms and recommendations to guide responsible behaviour by all parties in cyberspace, recently published its final report. The Cybersecurity Tech Accord aims to promote collaboration between tech companies on stability and resilience in cyberspace. President Macron’s ‘Paris Call for Trust and Security in Cyberspace’ has to date received the backing of 67 states, 139 international and civil society organizations, and 358 private-sector organizations.It remains to be seen in the long term whether the parallel processes at the UN will work constructively together or be competitive. But notwithstanding the challenging geopolitical backdrop, the UN GGE meeting next week at the least offers states the opportunity to consolidate and build on the results of past meetings; to increase knowledge and discussion about how international law might apply; and to encourage more states to put their own views of these issues on the record. Full Article
si 12-LOX catalyzes the oxidation of 2-arachidonoyl-lysolipids in platelets generating eicosanoid-lysolipids that are attenuated by iPLA2{gamma} knockout [Signal Transduction] By feedproxy.google.com Published On :: 2020-04-17T00:06:05-07:00 The canonical pathway of eicosanoid production in most mammalian cells is initiated by phospholipase A2-mediated release of arachidonic acid, followed by its enzymatic oxidation resulting in a vast array of eicosanoid products. However, recent work has demonstrated that the major phospholipase in mitochondria, iPLA2γ (patatin-like phospholipase domain containing 8 (PNPLA8)), possesses sn-1 specificity, with polyunsaturated fatty acids at the sn-2 position generating polyunsaturated sn-2-acyl lysophospholipids. Through strategic chemical derivatization, chiral chromatographic separation, and multistage tandem MS, here we first demonstrate that human platelet-type 12-lipoxygenase (12-LOX) can directly catalyze the regioselective and stereospecific oxidation of 2-arachidonoyl-lysophosphatidylcholine (2-AA-LPC) and 2-arachidonoyl-lysophosphatidylethanolamine (2-AA-LPE). Next, we identified these two eicosanoid-lysophospholipids in murine myocardium and in isolated platelets. Moreover, we observed robust increases in 2-AA-LPC, 2-AA-LPE, and their downstream 12-LOX oxidation products, 12(S)-HETE-LPC and 12(S)-HETE-LPE, in calcium ionophore (A23187)-stimulated murine platelets. Mechanistically, genetic ablation of iPLA2γ markedly decreased the calcium-stimulated production of 2-AA-LPC, 2-AA-LPE, and 12-HETE-lysophospholipids in mouse platelets. Importantly, a potent and selective 12-LOX inhibitor, ML355, significantly inhibited the production of 12-HETE-LPC and 12-HETE-LPE in activated platelets. Furthermore, we found that aging is accompanied by significant changes in 12-HETE-LPC in murine serum that were also markedly attenuated by iPLA2γ genetic ablation. Collectively, these results identify previously unknown iPLA2γ-initiated signaling pathways mediated by direct 12-LOX oxidation of 2-AA-LPC and 2-AA-LPE. This oxidation generates previously unrecognized eicosanoid-lysophospholipids that may serve as biomarkers for age-related diseases and could potentially be used as targets in therapeutic interventions. Full Article
si Glucocerebrosidases catalyze a transgalactosylation reaction that yields a newly-identified brain sterol metabolite, galactosylated cholesterol [Glycobiology and Extracellular Matrices] By feedproxy.google.com Published On :: 2020-04-17T00:06:05-07:00 β-Glucocerebrosidase (GBA) hydrolyzes glucosylceramide (GlcCer) to generate ceramide. Previously, we demonstrated that lysosomal GBA1 and nonlysosomal GBA2 possess not only GlcCer hydrolase activity, but also transglucosylation activity to transfer the glucose residue from GlcCer to cholesterol to form β-cholesterylglucoside (β-GlcChol) in vitro. β-GlcChol is a member of sterylglycosides present in diverse species. How GBA1 and GBA2 mediate β-GlcChol metabolism in the brain is unknown. Here, we purified and characterized sterylglycosides from rodent and fish brains. Although glucose is thought to be the sole carbohydrate component of sterylglycosides in vertebrates, structural analysis of rat brain sterylglycosides revealed the presence of galactosylated cholesterol (β-GalChol), in addition to β-GlcChol. Analyses of brain tissues from GBA2-deficient mice and GBA1- and/or GBA2-deficient Japanese rice fish (Oryzias latipes) revealed that GBA1 and GBA2 are responsible for β-GlcChol degradation and formation, respectively, and that both GBA1 and GBA2 are responsible for β-GalChol formation. Liquid chromatography–tandem MS revealed that β-GlcChol and β-GalChol are present throughout development from embryo to adult in the mouse brain. We found that β-GalChol expression depends on galactosylceramide (GalCer), and developmental onset of β-GalChol biosynthesis appeared to be during myelination. We also found that β-GlcChol and β-GalChol are secreted from neurons and glial cells in association with exosomes. In vitro enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form β-GalChol. This is the first report of the existence of β-GalChol in vertebrates and how β-GlcChol and β-GalChol are formed in the brain. Full Article
si Deletion of fatty acid transport protein 2 (FATP2) in the mouse liver changes the metabolic landscape by increasing the expression of PPAR{alpha}-regulated genes [Lipids] By feedproxy.google.com Published On :: 2020-04-24T06:08:45-07:00 Fatty acid transport protein 2 (FATP2) is highly expressed in the liver, small intestine, and kidney, where it functions in both the transport of exogenous long-chain fatty acids and the activation of very-long-chain fatty acids. Here, using a murine model, we investigated the phenotypic impacts of deleting FATP2, followed by a transcriptomic analysis using unbiased RNA-Seq to identify concomitant changes in the liver transcriptome. WT and FATP2-null (Fatp2−/−) mice (5 weeks) were maintained on a standard chow diet for 6 weeks. The Fatp2−/− mice had reduced weight gain, lowered serum triglyceride, and increased serum cholesterol levels and attenuated dietary fatty acid absorption. Transcriptomic analysis of the liver revealed 258 differentially expressed genes in male Fatp2−/− mice and a total of 91 in female Fatp2−/− mice. These genes mapped to the following gene ontology categories: fatty acid degradation, peroxisome biogenesis, fatty acid synthesis, and retinol and arachidonic acid metabolism. Targeted RT-quantitative PCR verified the altered expression of selected genes. Of note, most of the genes with increased expression were known to be regulated by peroxisome proliferator–activated receptor α (PPARα), suggesting that FATP2 activity is linked to a PPARα-specific proximal ligand. Targeted metabolomic experiments in the Fatp2−/− liver revealed increases of total C16:0, C16:1, and C18:1 fatty acids; increases in lipoxin A4 and prostaglandin J2; and a decrease in 20-hydroxyeicosatetraenoic acid. We conclude that the expression of FATP2 in the liver broadly affects the metabolic landscape through PPARα, indicating that FATP2 provides an important role in liver lipid metabolism through its transport or activation activities. Full Article
si The transcriptional regulator MEIS2 sets up the ground state for palatal osteogenesis in mice [Gene Regulation] By feedproxy.google.com Published On :: 2020-04-17T00:06:05-07:00 Haploinsufficiency of Meis homeobox 2 (MEIS2), encoding a transcriptional regulator, is associated with human cleft palate, and Meis2 inactivation leads to abnormal palate development in mice, implicating MEIS2 functions in palate development. However, its functional mechanisms remain unknown. Here we observed widespread MEIS2 expression in the developing palate in mice. Wnt1Cre-mediated Meis2 inactivation in cranial neural crest cells led to a secondary palate cleft. Importantly, about half of the Wnt1Cre;Meis2f/f mice exhibited a submucous cleft, providing a model for studying palatal bone formation and patterning. Consistent with complete absence of palatal bones, the results from integrative analyses of MEIS2 by ChIP sequencing, RNA-Seq, and an assay for transposase-accessible chromatin sequencing identified key osteogenic genes regulated directly by MEIS2, indicating that it plays a fundamental role in palatal osteogenesis. De novo motif analysis uncovered that the MEIS2-bound regions are highly enriched in binding motifs for several key osteogenic transcription factors, particularly short stature homeobox 2 (SHOX2). Comparative ChIP sequencing analyses revealed genome-wide co-occupancy of MEIS2 and SHOX2 in addition to their colocalization in the developing palate and physical interaction, suggesting that SHOX2 and MEIS2 functionally interact. However, although SHOX2 was required for proper palatal bone formation and was a direct downstream target of MEIS2, Shox2 overexpression failed to rescue the palatal bone defects in a Meis2-mutant background. These results, together with the fact that Meis2 expression is associated with high osteogenic potential and required for chromatin accessibility of osteogenic genes, support a vital function of MEIS2 in setting up a ground state for palatal osteogenesis. Full Article
si Certain ortho-hydroxylated brominated ethers are promiscuous kinase inhibitors that impair neuronal signaling and neurodevelopmental processes [Cell Biology] By feedproxy.google.com Published On :: 2020-05-01T00:06:09-07:00 The developing nervous system is remarkably sensitive to environmental signals, including disruptive toxins, such as polybrominated diphenyl ethers (PBDEs). PBDEs are an environmentally pervasive class of brominated flame retardants whose neurodevelopmental toxicity mechanisms remain largely unclear. Using dissociated cortical neurons from embryonic Rattus norvegicus, we found here that chronic exposure to 6-OH–BDE-47, one of the most prevalent hydroxylated PBDE metabolites, suppresses both spontaneous and evoked neuronal electrical activity. On the basis of our previous work on mitogen-activated protein kinase (MAPK)/extracellular signal-related kinase (ERK) (MEK) biology and our observation that 6-OH–BDE-47 is structurally similar to kinase inhibitors, we hypothesized that certain hydroxylated PBDEs mediate neurotoxicity, at least in part, by impairing the MEK–ERK axis of MAPK signal transduction. We tested this hypothesis on three experimental platforms: 1) in silico, where modeling ligand–protein docking suggested that 6-OH–BDE-47 is a promiscuous ATP-competitive kinase inhibitor; 2) in vitro in dissociated neurons, where 6-OH–BDE-47 and another specific hydroxylated BDE metabolite similarly impaired phosphorylation of MEK/ERK1/2 and activity-induced transcription of a neuronal immediate early gene; and 3) in vivo in Drosophila melanogaster, where developmental exposures to 6-OH–BDE-47 and a MAPK inhibitor resulted in offspring displaying similarly increased frequency of mushroom-body β–lobe midline crossing, a metric of axonal guidance. Taken together, our results support that certain ortho-hydroxylated PBDE metabolites are promiscuous kinase inhibitors and can cause disruptions of critical neurodevelopmental processes, including neuronal electrical activity, pre-synaptic functions, MEK–ERK signaling, and axonal guidance. Full Article
si The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis [Cell Biology] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 Sperm head shaping is a key event in spermiogenesis and is tightly controlled via the acrosome–manchette network. Linker of nucleoskeleton and cytoskeleton (LINC) complexes consist of Sad1 and UNC84 domain–containing (SUN) and Klarsicht/ANC-1/Syne-1 homology (KASH) domain proteins and form conserved nuclear envelope bridges implicated in transducing mechanical forces from the manchette to sculpt sperm nuclei into a hook-like shape. However, the role of LINC complexes in sperm head shaping is still poorly understood. Here we assessed the role of SUN3, a testis-specific LINC component harboring a conserved SUN domain, in spermiogenesis. We show that CRISPR/Cas9-generated Sun3 knockout male mice are infertile, displaying drastically reduced sperm counts and a globozoospermia-like phenotype, including a missing, mislocalized, or fragmented acrosome, as well as multiple defects in sperm flagella. Further examination revealed that the sperm head abnormalities are apparent at step 9 and that the sperm nuclei fail to elongate because of the absence of manchette microtubules and perinuclear rings. These observations indicate that Sun3 deletion likely impairs the ability of the LINC complex to transduce the cytoskeletal force to the nuclear envelope, required for sperm head elongation. We also found that SUN3 interacts with SUN4 in mouse testes and that the level of SUN4 proteins is drastically reduced in Sun3-null mice. Altogether, our results indicate that SUN3 is essential for sperm head shaping and male fertility, providing molecular clues regarding the underlying pathology of the globozoospermia-like phenotype. Full Article
si WYSIWYG Web Builder 7.0 released! By www.wysiwygwebbuilder.com Published On :: Wed, 23 Jun 2010 10:40:59 GMT We are pleased to announce a major new release with more than 150 new features and improvements! Full Article
si WYSIWYG Web Builder 7.5 available now! By www.wysiwygwebbuilder.com Published On :: Mon, 20 Dec 2010 10:58:56 GMT WYSIWYG Web Builder 7.5 is a major update with more than 50 new features and improvements (compared to version 7.2.1). Our christmas gift to you! Full Article
si WYSIWYG Web Builder 8.0 released! By www.wysiwygwebbuilder.com Published On :: Mon, 21 Nov 2011 12:08:05 GMT We are pleased to announce a major new release with more than 150 new features and improvements! Full Article
si WYSIWYG Web Builder 8.5 available now! By www.wysiwygwebbuilder.com Published On :: Mon, 01 Oct 2012 08:23:59 GMT WYSIWYG Web Builder 8.5 is a major update with more than 50 new features and improvements (compared to version 8.2.1). Full Article
si WYSIWYG Web Builder 9.0 released! By www.wysiwygwebbuilder.com Published On :: Mon, 17 Jun 2013 10:53:50 GMT We are pleased to announce a major new release with more than 150 new features and improvements! Full Article
si WYSIWYG Web Builder 9.1 update By www.wysiwygwebbuilder.com Published On :: Mon, 23 Sep 2013 09:35:20 GMT WYSIWYG Web Builder 9.1 fixes known problems, adds new features and includes other improvements. Full Article