Expert

18 August 2020

Poly(ADP-ribose) polymerase (PARP) superfamily members covalently link either a single ADP-ribose (ADPR) or a chain of ADPR units to proteins using NAD as the source of ADPR. Although the well-known poly(ADP-ribosylating) (PARylating) PARPs primarily function in the DNA damage response, many noncanonical mono(ADP-ribosylating) (MARylating) PARPs are associated with cellular antiviral responses. We recently demonstrated robust up-regulation of several PARPs following infection with murine hepatitis virus (MHV), a model coronavirus. Here we show that SARS-CoV-2 infection strikingly up-regulates MARylating PARPs and induces the expression of genes encoding enzymes for salvage NAD synthesis from nicotinamide (NAM) and nicotinamide riboside (NR), while down-regulating other NAD biosynthetic pathways. We show that overexpression of PARP10 is sufficient to depress cellular NAD and that the activities of the transcriptionally induced enzymes PARP7, PARP10, PARP12 and PARP14 are limited by cellular NAD and can be enhanced by pharmacological activation of NAD synthesis. We further demonstrate that infection with MHV induces a severe attack on host cell NAD+ and NADP+. Finally, we show that NAMPT activation, NAM, and NR dramatically decrease the replication of an MHV that is sensitive to PARP activity. These data suggest that the antiviral activities of noncanonical PARP isozyme activities are limited by the availability of NAD and that nutritional and pharmacological interventions to enhance NAD levels may boost innate immunity to coronaviruses.

Nearly everyone in behavioral marketing and influence marketing was surprised when Dr. Robert Cialdini, the “godfather” of persuasion science and the creator of the celebrated Six Principles of Influence, introduced a seventh principle, Unity. In fact, in 2014 I asked Cialdini if, thirty years after completing his seminal book, Influence, he’d add on another one or […]

The post What is Unity, Cialdini’s 7th Principle? appeared first on Neuromarketing.

Three Oregon school employees sued their union in federal court last week, arguing it's unfair that the teachers' union only lets members drop out and stop paying dues during the month of September.

Florida's teachers are marching on Tallahassee today. Hard-working teachers deserve a big raise and talented teachers are profoundly underpaid. But teachers ought not overplay their hand, or they're likely to face a backlash of their own.

A Tallahassee, Fla., K-8 school is mourning two staff members and a former employee. All of them recently died from the virus.

Controversy over an intiative aimed a reducing inequities in Lee's Summit, Mo., schools led the police department to provide security protection for the district's first African-American superintendent. Now the school board has reversed course.

Primary season is now upon us. Here are three ideas for teaching in the wake of the Iowa caucus fallout.

Could the Islanders potentially find themselves in a wild-card spot on Wednesday morning? In this economy?

World Food Day is coming up this Monday, October 16. This year’s theme, Change the future of migration. Invest in food security and rural development, highlights FAO’s work in [...]

CBC Newfoundland and Labrador would love to hear your suggestions of folks and organizations who are there when you need them — and all the other times, too.

There have been three separate reports of pellet gun attacks in the past seven days in Guelph, Ont. Police have made an arrest in one case and continue to investigate the other two.

We've heard what the candidates want to do about community safety in Saskatoon. Here's what the people already doing the work say the new mayor and council should do after the civic election.

The Pinecrest Queensway and Somerset West health centres are aiming to get a slice of $370 million of provincial funding to operate centres focused on addictions recovery and links to supportive housing.

The re-elected chief of the Tk'emlúps te Secwépemc First Nation says she's hoping to forge connections within her community as she looks ahead to her next term.

Can young people be united in faith, irrespective of their ethnic background? The answer is yes!

Eliminates the Cost and Complexity of Maintaining Online Parts Catalogs; Improves Search, Navigation, and Communication for Engineers Seeking 3D Part Models

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People who want to support the well-being of youth and families in their communities can register for free online courses through Penn State’s Prevention Learning Portal. The portal offers instruction and resources on how to choose, implement and sustain programs and practices that promote healthy communities.

A team of OM Dancelink dancers in Portugal impact the community through workshops, shows and street performances.

OMers work with a Hungarian church to invest in relationships through every season.

Hungarian students learn about dating, sex and marriage during a Bus4Life visit.

A short-termer engages people in spiritual conversation while on tour of Israel.

OM's vocational training centres offer valuable skills to women and youth and see the development of character and community.

Extreme Leadership Training camps create unity in Ukraine.

Puntarenas, Costa Rica :: A group of indigenous people from the Talamanca region have a horizon-expanding visit to Logos Hope.

At a recent event to build relationships, OMer Chris guides participants through the process of making juggling balls before teaching them how to juggle.

Kacey Harper, a third-year corporate communication major at Penn State Schuylkill, discovered her passion for the field after initially considering marketing. She honed her skills through various leadership roles on campus and a hands-on internship with Schuylkill United Way. Harper is eager to pursue a career that allows her to make meaningful contributions, and she encourages others to gain as much professional experience as possible through Schuylkill’s Co-Op program.

Emily Weider, a third-year student at Penn State Schuylkill, has always been drawn to business, growing up involved in her family's trucking company. Her Co-Op experience at Northeast Pennsylvania Manufacturers and Employers Association provided valuable hands-on learning, from managing databases to enhancing marketing efforts with Canva. The welcoming environment and the skills she gained have solidified her confidence in pursuing a future in business.

Join Penn State Great Valley at the campus' Conference Center in Malvern on Oct. 24 for an electrifying evening in the vibrant world of Bollywood, where the magic of Indian cinema will be celebrated.

A physical therapy clinic — staffed by students supervised by licensed professionals and offering free services to volunteer community participants in need — is planned for fall 2025 at Pennsylvania College of Technology.

Penn State announced the recipients of the inaugural Commonwealth Campus Undergraduate Community-Engaged Research Awards, a new program designed for faculty who specifically support undergraduate student participation in research that aims to improve community well-being.

Penn State Hazleton's trick-or-treating is scheduled for Thursday, Oct. 24, from 5 to 6:30 p.m. on the Sports and Recreation Field at the campus. Children attending the event must be accompanied by a parent or guardian. 

Penn State Berks wrapped up its celebration of Hispanic Heritage Month on Oct. 11 with its 12th annual Latino Forum, an outreach effort for local high school students. Chancellor Radha Pyati provided a warm welcome to the students in Spanish, and she reached out to both students and the greater Latino community through a Facebook Live interview on La Mega Radio Station, a Spanish-language station who was on site for the event. Pyati was also interviewed live on La Mega’s Morning Show on Oct. 8.

How do you translate what you learned in a semester-long course to a community celebration for an audience ranging in age from kindergarteners to senior citizens? That was the challenge to Penn State Berks students in the “Taylor Swift, Gender and Communication” course, and they rose to the occasion.

Penn State College of Medicine and Huck Institutes of the Life Sciences are launching a new Grand Rounds topic: "Artificial Intelligence in Health."

Join Penn State Altoona as it kicks off Veteran Appreciation Week with a community movie night on Saturday, Nov. 9, at the Devorris Downtown Center in downtown Altoona. The first family-friendly movie, “Hotel Transylvania,” will begin at 5:30 p.m. The second adult-themed movie is “Pineapple Express,” which will begin between 7:30 and 8 p.m.

“Inside Out 2” will be the featured film for the drive-in movie at Penn State York set for 8 p.m. Friday, Sept. 13. The film is free and open to the public. Guests can park in the lower parking lot area behind the Pullo Family Performing Arts Center on campus beginning at 6 p.m. 

Liam Geyer, a fifth-year Integrated Undergraduate/Graduate student majoring in cybersecurity analytics and operations, leveraged his involvement in the Competitive Cybersecurity Organization at Penn State to land internships and full-time employment. 

As Donald Trump prepares to make good on his promise to deport possibly millions of undocumented immigrants, the private prison industry appears poised to cash in.

The Brandywine community showed its Penn State pride at the campus' annual Homecoming & Family Day on Oct. 26. The day featured carnival games, an alumni reception, face painting, a petting zoo and more.

Penn State World Campus will accept credits from certain courses offered by the Community College of the Air Force that can be transferred toward a bachelor of science in organizational leadership. The degree is offered online through Penn State World Campus by the School of Labor and Employment Relations in the College of the Liberal Arts.

Penn State Law welcomed the LL.M. (master of laws) class of 2025 to the University Park campus for orientation on Aug. 20, kicking off another year of one of the largest and most diverse LL.M. programs in the United States. The class of 2025 includes 163 LL.M. and 13 exchange students, coming from 31 countries across the world.

Alumnus Pablo Lopez works to give back to Penn State and State College through his role as film production manager for the Happy Valley Adventure Bureau.