In one weekend Stoffel Vandoorne manages to qualify on pole position in two races and crash out of both of them.

How the global pandemic is limiting carbon emissions and what this will mean for climate change.

Some believe that self-driving cars will be far safer than human-driven cars but who trusts them enough to drive in them?

Former defence minister says cargo plane shot down as it tried to land in the southern town of Bardale.

A Chicago cyclist was injured by an electric scooter rider who fled the scene. Now he's gone to court to get the city's scooter companies to turn over personal information about their riders and their history.

A horror crash involving a truck and a tanker carrying a flammable substance has led to the closure of the N2, near the Mtunzini Toll Plaza on the KwaZulu-Natal north coast.

One day, a teacher entered her classroom and placed a glass jar on the table. She put two large rocks into the jar until no more could fit. She asked the class if the jar was full and they all said, “Yes.” She responded, “Really?” She pulled out a pile of small pebbles, added them […]

The post How to Become Great by Managing Procrastination appeared first on Dumb Little Man.

The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

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The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

Host Matt Egan is joined this week by Henry Burrell, staff writer at Macworld UK to talk about the EU referendum website crashing Lewis Painter, staff writer at PC Advisor, jumps in to chat about Oculus Rift games and how immersive they are. Finally regular David Price comes on to talk about Apple's upcoming WWDC software developer conference and what we can (and cannot) expect.  

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Research Event

Event participants

Dr Jon Gibbins, Senior Lecturer in the Department of Mechanical Engineering, Imperial College London
Jim Footner, Senior Climate Change Campaigner, Greenpeace

Emotions remain raw as educators and residents in a rural Wisconsin district dig for solutions after being denied the option of dissolving.

Wien : Kaulfuss Witwe, Prandel, 1844.

An Essex County OPP officer has been slapped with a cut in pay because of "inexplicable" investigative delays into a fatal collision, allowing the at-fault driver to "evade criminal prosecution."

A young fan of the Jurassic Park film franchise sporting a Tyrannosaurus Rex costume got to meet some of the cast and crew who have been filming the latest instalment of the franchise in his hometown of Merritt, B.C.

European Systemic Risk Board Occasional Papers by Greg Ros

What’s bad for America is sometimes good for the market.

A number of popular iOS apps, including TikTok, Pinterest and Spotify, began to crash on Wednesday after an update to Facebook's SDK rolled out, software that many developers rely on to streamline the user login process.

WHAT a difference a Prime Minister makes. There used to be a time, back before it wasn’t an obstacle to promotion, that Jackson Carlaw was aghast at the idea of a no-deal Brexit.

Are you in your twenties and wonder how you can use the current market crash caused by fears related to the coronavirus pandemic as a way to get into the investment market?

The Model 3 was on Autopilot and managed to rear-end the police car which had stopped to assist a disabled vehicle and had its emergency lights activated.

The 26 pound drone and its cargo fell to the ground after somehow managing to sever its own parachute rope. It landed 150 feet away from a group of children.

Our series focusing on the big moments in EURO history and the people involved reaches 1992, and the final act of last-minute entrants Denmark's fairy-tale summer.

A Maryland woman who caused a fatal crash while driving under the influence of drugs has been sentenced to prison. Deputy Attorneys General Barzilai Axelrod and William Leonard secured the sentence for Michelle Park, 35, of Earlville. In March 2018, with her 2-year-old child in the car, Park failed to negotiate a curve while driving […]

Kobe and Gianna Bryant were among nine people who died in the January 26 helicopter crash in the mountains west of Los Angeles.

Meanwhile, domestic consumption of petroleum products in FY20 remained flat at 213.7 MT as sales of transportation fuels plummeted in March amid the country-wide lockdown.

  Delaware– The Delaware State Police have seen a recent spike in fatal crashes across the entire state.  In conjunction with the Delaware Office of Highways Safety, we are working together to provide our citizens with a few traffic safety reminders. The majority of fatal crashes are attributed to some form of distracted driving.  This […]

According to Crisil estimates, the aviation industry will crash-land this fiscal with revenue loss of Rs 24,000-25,000 crore during the April-June quarter.

The IIHS reported Thursday that the new Jeep Wrangler, which was last comprehensively updated for the 2018 model year, tipped over onto its passenger side during its driver-side small overlap crash test. The insurance industry-funded safety watchdog said the Wrangler was tested three times, twice by the agency and once by the automaker. The...

Tesla Inc. was sued by the family of a man who died as the result of a crash allegedly caused when the Autopilot navigation system of his 2017 Model X malfunctioned.

Police have released the name of the man who died following a crash on State Highway 51, Napier, on Thursday.He was David Allan Trousdell, 37, of Napier.It's understood Mr Trousdell's car collided with a truck at Te Awa. The crash...

A horror crash involving a truck and a tanker carrying a flammable substance has led to the closure of the N2, near the Mtunzini Toll Plaza on the KwaZulu-Natal north coast. ......

TROOP D NEWS RELEASE May 9, 2020 **UPDATE** Driver and Passenger Killed in Head-On Crash in Calcasieu Parish Sulphur, LA. - The deceased passenger is identified as 38-year-old Michael C. Vidrine of Eunice, LA. Original Release - On May 8, 2020, at approximately 4:15 p.m., Troopers from Louisiana State Police Troop D responded to a two-vehicle, fatal crash on LA Hwy 27 on top of...

[Dalsan Radio] 2 Kenyan pilots, a Kenyan flight engineer and 3 Somalia national were on Monday killed when their plane was hit and crashed near Bardale in Somalia. The cargo plane a Eubreur 120 registered 5Y AXO belonging to African Express was crashed 5km to Bardale Airstrip.

[Dalsan Radio] A plane carrying aid supplies for use in the fight against the coronavirus crashed in Somalia on Monday, killing all six people on board, the Somali transport minister said.

[DW] Kenya has demanded an investigation into a fatal crash of a humanitarian aircraft which killed all people on board.

[Shabelle] Kenya on Tuesday called for an urgent investigation into the fatal crash of a private cargo plane in Somalia amid unconfirmed reports it may have been shot down.

[Nation] The Federal Government of Somalia started an investigation into a plane that crashed on Monday near the airstrip of Bardale town in Bay region, about 275 kilometres southwest of the capital Mogadishu.