The province is still on track to move to a lower alert level Monday, which would ease some of the public health measures in place aimed at curbing COVID-19.

64-year-old John Neville, in agony and needing his wisdom tooth extracted, has been navigating a complex tangle of COVID-19 restrictions in an unsuccessful attempt at getting emergency dental care.

Buenos Aires, Argentina :: Miembros de la comunidad japonesa encuentran amigos en un barco de visita y escuchan el evangelio en su lengua materna.

An OM worker from Latin America uses his carpentry skills to disciple young men in their faith and give them better employment opportunities for the future.

Buenos Aires, Argentina :: Maintenance crew share Christ's love with local welders helping repair Logos Hope.

From deep bass notes echoing through the speaker system, to the countries welcome video, to moments of voices lifted up in praise, the opening session of TeenStreet 2019 made a powerful impact on the participants.

La tripulación de técnicos del Logos Hope sabe que el mantenimiento del barco es una forma de hacer ministerio.

When Elizabeth May, parliamentary leader of the federal Green Party, proclaimed on Tuesday that "oil is dead," she was correct in a philosophical sense. But not in a practical, real world sense.

People and businesses are rallying to support the Muslim community in Fort McMurray recover after widespread flooding that damaged many neighbourhoods.

Disaster relief funding for punishing spring floods is welcome news to civic leaders in northern Alberta.

Before COVID-19 hit, Hemliss Eloïse Konan had plans for how she'd spend her summer in Sudbury. After finishing her first year at Laurentian University, Konan planned to stay in residence, and get a job for the summer.

Tensions are rising on Manitoulin Island because a First Nation is stopping travellers on provincial highways that go through the community. But opinions on M'Chigeeng's attempt to protect its people from COVID-19 are not divided along racial lines. 

The Porcupine Health Unit is reporting the fifth COVID-19 related death in its area.

Business owners throughout northern Ontario will have the chance Friday morning to speak directly with federal Economic Development Minister Mélanie Joly.

A former miner who was forced to breathe in a fine aluminum dust before each shift says he’s not surprised a new report links that dust and a higher risk of Parkinson’s disease.

The following is a guest post by Martha H. Kennedy, Curator of Popular & Applied Graphic Art, Prints & Photographs Division. During Will Eisner Week, March 1-7, 2020, the Library of Congress joins art schools, libraries, universities, and museums in a global celebration of this legendary creator’s contributions to the world of comic art and […]

The following is a guest post by Hanna Soltys, Reference Librarian, Prints & Photographs Division. The post was written with the help of Sara W. Duke, Curator of Popular and Applied Graphic Art.   While professional baseball’s Opening Day will take place at a later date, the spirit and excitement of the day still live […]

This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.

Over many years, the Xenopus laevis embryo has provided a powerful model system to investigate how mechanical forces regulate cellular function. Here, we describe a system to apply reproducible tensile and compressive force to X. laevis animal cap tissue explants and to simultaneously assess cellular behavior using live confocal imaging.

In this protocol, DNA fragments are separated according to size by electrophoresis through low-melting-temperature agarose, and then recovered by melting the agarose and extracting with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 to 5.0 kb. Yields of DNA fragments outside this range are usually lower, but often are sufficient for many purposes.

Mice, rats, or hamsters are immunized by giving biweekly injections of a purified antigen, cultured cells, or cDNA. For mice, if a pure, soluble protein antigen is being used and is abundant, a dose of 50–100 µg in adjuvant at each immunization is a sensible general recommendation; for rats and hamsters, a dose of 100–200 µg is sufficient. Lower doses can be used for antigens with higher immunogenicity. Adjuvants (Freund's, Ribi, Hunter's TiterMax, ImmunEasy, or Alum) should be mixed with the immunizing antigen for the first two immunizations only; Complete Freund's adjuvant is only used with the first immunization. Subsequent immunizations are performed in phosphate-buffered saline (PBS) or normal saline, with or without Incomplete Freund's adjuvant. The choice of adjuvant is dependent on the subclass of immunoglobulin required. Over the course of the 6-wk immunization schedule, each animal usually receives a total of six injections (three subcutaneous and three intraperitoneal). Once a good titer has developed against the antigen of interest, regular boosts and bleeds are performed to collect the maximum amount of serum. For rats and hamsters, boosts should be spaced every 2–3 wk, and serum samples of 400–500 µL should be collected 10–12 d after each boost. For mice, boosts should be spaced every 2–3 wk, and serum samples of 200–300 µL should be collected 10–12 d after each boost.

Before probing blots for the presence of an antigen, the total composition of the transferred proteins can be determined by staining the nitrocellulose or polyvinylidene fluoride (PVDF) membrane. Staining for proteins is useful to determine the position of the non-prestained molecular weight markers or individual lanes on the gel and to ensure that efficient transfer has occurred. It can be also used to verify equal loading of the samples in the gel when a comparison of the protein of interest between the different samples is important. The conventional procedures such as Coomassie Blue and silver staining methods used for staining polyacrylamide gels are incompatible with immunoblotting. Ponceau S is the more common staining method in immunoblotting protocols because it is compatible with antibody–antigen binding, is cost efficient, and provides a good contrast between the stained bands and background. In this protocol, nitrocellulose or PVDF membrane is rinsed with ultrapure H₂O after the transfer of proteins. Ponceau S dye is applied as an acidic aqueous solution, and the proteins on the membrane are stained with red color. The membrane is briefly destained with water and can be photographed or scanned to obtain the image of the total protein staining. Individual lane positions or the molecular weight standards can be marked with a pencil, if required.

The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

This protocol describes the preparation of the shuttle vector before its introduction into bacterial artificial chromosome (BAC) host cells for BAC two-step engineering. The homology arm sequences, prepared previously, are introduced by ligation into the digested shuttle vector DNA to provide sites for recombination within the BAC clone. Crude lysates of individual bacterial transformants serve as templates in polymerase chain reaction (PCR) analysis to confirm the presence of the homology arms in the recombinant shuttle vector.

FSI Briefs No 3, April 2020. In response to the 2007-09 Great Financial Crisis (GFC), accounting standard setters introduced a new methodology to value loans based on expected credit losses (ECL). The previous approach, based on incurred losses, was viewed as procyclical and inconsistent with prudential objectives. In the wake of the Covid-19 pandemic, several prudential authorities and the Basel Committee on Banking Supervision (BCBS), introduced a series of measures to clarify how banks should consider various public and private debt relief programmes in their ECL estimates and in their calculation of regulatory capital. These measures are intended to incentivise banks to continue supporting the real economy, while reducing pressure on banks' ECL provisions, earnings and regulatory capital. Supervisory initiatives that provide capital relief should be augmented by severe constraints on the payment of dividends, bonuses and share buybacks. These joint actions will simultaneously expand banks' lending capacity and enhance their ability to absorb losses. Prudential authorities face difficult trade-offs as they confront the most severe economic crisis in modern times. Encouraging the use of flexibility in applicable accounting standards, while preserving market trust and transparency in the reported financial statements of banks, will be key in fostering both economic and financial stability.

A prototype deployed in San Francisco Bay imagines the underside of a floating building as an upside-down artificial reef

No global real estate market despite higher price synchronisation and growing role of international investors, central banks find (Press release, 18 February 2020)

Basel Committee Press release "Basel Committee meets to review vulnerabilities and emerging risks, advance supervisory initiatives and promote Basel III implementationl", 27 February 2020.

BCBS Press release "Basel III monitoring results based on end-June 2019 data published by the Basel Committee", 8 April 2020

CPMI Press release "How fintech can promote financial inclusion - a new report on the opportunities and challenges", 14 April 2020

A randomized, controlled clinical trial evaluating the safety and efficacy of a treatment regimen of the investigational antiviral remdesivir plus the anti-inflammatory drug baricitinib for coronavirus disease 2019 (COVID-19) has begun.

It’s the coronavirus election now.

We need to recognize any signposts leading up and out.

When the province eventually conducts its review on the response to COVID-19, the president of the Nova Scotia Nurses’ Union can think of one thing in particular that needs to be discussed.

Nova Scotia students and teachers will not be returning to the classroom this year. At-home learning will continue until June 5, when the province's school year will end.

Quebecers, like the rest of the world, are growing accustomed to the uncertainty that's accompanied the pandemic. But they may not appreciate their government adding to that already hefty burden.

The provincially appointed trustee of the English Montreal School Board says the Quebec government made the “right decision” when it postponed the reopening of Montreal-area elementary schools to May 25.

As the Montreal area continues to be the Canadian epicentre of the COVID-19 outbreak, anyone showing symptoms of the virus is being asked to get tested.

While the STM is strongly urging passengers to wear a face mask or face covering at all times, it won't be mandatory, said chair Philippe Schnobb.

For close to 15 years, Reema Singh has been baking and selling cakes from her tiny shop on Parc Avenue in Montreal's Mile End. Cocoa Locale has been open throughout the pandemic because — well, it turns out that cake is essential.

Jean-Talon market has changed during the COVID-19 pandemic. Today, there are controlled entrances, someone making sure you douse your hands with sanitizer and another with a clicker in hand, counting the number of people who enter.

Sheltering in place doesn’t mean you have to give up the best of wine country's offerings

Baltimore Orioles outfielder and first baseman Trey Mancini revealed Tuesday he is in the midst of six months of chemotherapy as he fights Stage III colon cancer.

Major League Baseball could hold a rescheduled opening day between mid-June and July 4 and play at least 80 games this season, The Athletic's Ken Rosenthal reported Tuesday.

The new baseball season began in South Korea on Tuesday with the crack of the bat and the sound of the ball smacking into the catcher's mitt echoing around empty stadiums.

The Cleveland Indians held a video conference with players and other team officials to lay out the provisional plan for returning to organized workouts with designs on being ready for a potential 2020 opening day of July 1, according to The Athletic.

Making Tierra Mía, says the director of the Smithsonian Latino Center, proved transformative in giving voice to the people

The University of Manitoba is laying off dozens of employees as it tries to find savings at a time when post-secondary schools have largely been closed since COVID-19 emerged on the Prairies, a union says.

A Winnipeg School Division trustee who was suspended from the school board earlier this week does not plan on fighting the decision, she told CBC News.