Infections with nontuberculous mycobacteria (NTM) have a poor prognosis in patients with underlying respiratory diseases. Clofazimine (CFZ) showed both experimental and clinical promising results against clinically relevant NTM. However, there are no data on CFZ in combination with the current recommended treatment; therefore, we aimed to study its in vivo activity in an aerosol mouse model of Mycobacterium avium. In an aerosol infection BALB/c mouse model using M. avium strain Chester, we treated 58 mice with four combinations of rifampin (RIF) at 10 mg/kg, CFZ at 25 mg/kg, and clarithromycin (CLR) and ethambutol (EMB) at 100 mg/kg. Treatment efficacy was assessed on the basis of lung CFU counts after 2 (M2) and 4 (M4) months of treatment. At M2, CLR-RIF-EMB was slightly but significantly more efficient than CFZ-RIF-EMB (3.02 ± 0.12 versus 3.55 ± 0.28, respectively, P < 0.01), whereas CLR-CFZ-EMB and CLR-CFZ-RIF-EMB dramatically decreased lung CFU counts by 4.32 and 4.47 log₁₀, respectively, compared to untreated group. At M4, CLR-RIF-EMB was significantly more efficient than CFZ-RIF-EMB (2 ± 0.53 versus 2.66 ± 0.22, respectively, P = 0.01). The addition of CLZ to CLR dramatically decreased the lung CFU count, with CFU counts 5.41 and 5.79 log₁₀ lower in the CLR-CFZ-EMB and CLR-CFZ-RIF-EMB groups, respectively, than in the untreated group. The addition of CFZ to CLR seems to improve the efficacy of CLR as early as M2 and was confirmed at M4. CFZ, in addition to RIF and EMB, on the other hand, is less effective than CLR-RIF-EMB. These results need to be confirmed by similar studies along with CFZ potential for shortening treatment.

Royal Enfield अपनी Classic 350 का न्यू जनरेशन मॉडल लॉन्च करने की तैयारी कर रही है। जानकारी के मुताबिक कंपनी इस मॉडल को इस साल के अंत तक या अगले साल के शुरुआत में ही लॉन्च कर सकती है।

Flying car hopefuls were meant to take off at a competition on a NASA airbase, but no team claimed the prize after a string of crashes and no-shows

The company could be preparing to release a new iMac, a new pair of AirPods, and an updated Apple TV 4K.

This unusual contraption recreates the sounds of an old-school mechanical typewriter on a modern keyboard.

This film gives us insight into a community that’s already endured plenty even before the zombies arrived.

This clay robot can squeeze and squish itself into different shapes

Interrogate the data, consider possible incentives, and look for the dreaded “hype salad”

Shannon's said: “OCRO HLI RGWR NMIELWIS”

It’s almost here! We play your…insightful E3 voicemails. Then we give our…questionable E3 predictions. Finally, we give you some E3 plushies just for you, our...

Platform sandals meet 40-denier tights as rock’n’roll goes bourgeois at Paris fashion week

At his Celine catwalk show on Friday night, the designer Hedi Slimane distilled what looks to be the most compelling trend of this season of fashion shows. Working title: rock’n’roll bourgeois.

Slim knee-length dresses were worn with slinky chain belts; towering platform sandals with sensible 40-denier semi-sheer tights; slim bootcut velvet trousers worn with Princess-line above-the-knee coats. This was a masterclass in Parisian allure: take one part Avenue Montaigne privilege, and mix with one part backstage pass. Shaken, not stirred.

To avoid arsenic contamination, many Bangladeshi households access water via private wells drilled to 300 feet or less, beneath impermeable clay layers. Such clay layers have been thought to protect groundwater in the underlying aquifers from the downward flow of contaminants. However, a new study suggests that such clay layers do not always protect against arsenic, and could even be a source of contamination in some wells.

Excellent film-maker that he is, Watiti seems to fit the Marvel blueprint far more easily than he does Star Wars’ more venerable, old-school template

When entertainment reporters play Hollywood roulette, the practice of attaching directors and stars to forthcoming movies based on little more than rumour, their little white balls nearly always seem to land on Taika Waititi’s number. If you’ve been keeping a close eye on this column over the past year, you’ve probably spotted the white-hot Kiwi director being touted for a remake of Flash Gordon and the next Deadpool movie among other projects, neither of which have yet come to fruition.

Waititi’s next film, according to reports this week, will be a Star Wars episode. Will he end up making it to the first day of production on this one? The chances seem better, as Disney has officially confirmed the appointment via the space saga’s official website, with 1917 co-writer Krysty Wilson-Cairns helping deliver a script. But this is Star Wars we are talking about – Colin Trevorrow, Josh Trank, Phil Lord and Christopher Miller, David Benioff and DB Weiss are among the numerous film-makers who have cheerily signed up to try to bring back the glory days of the long-running series in recent times, only to ultimately fall foul of Lucasfilm president Kathleen Kennedy’s merciless Force choke.

Bill Maher said Republicans "don't care" about Tara Reade's sexual assault allegations, saying such claims are simply used as a "unilateral weapon" against Democrats.

The BJP leaders waged a social media war against the Bengal government by accusing the state of hiding Covid-19 figures and also complaining the Centre that TMC-led government in Bengal has completely failed in handling the coronavirus crisis.

The BJP leaders waged a social media war against the Bengal government by accusing the state of hiding Covid-19 figures and also complaining the Centre that TMC-led government in Bengal has completely failed in handling the coronavirus crisis.

The DRC’s Ebola epidemic will be declared over on 12 April but there’s little time to celebrate as the country pivots towards tackling the coronavirus

The Netherlands international has become a talismanic presence for club country, but the commanding centre-half believes there is more to come

Damien Comolli, who took the England international to Anfield, saw the Reds enter into transfer talks with the Cottagers during the summer of 2012

Kal Penn and The Walking Dead's Michael Cudlitz join Lecter-less sequel show.

The novel coronavirus -- which has infected over 3.9 million people worldwide -- has been detected in wastewater, but researchers have been unclear about the transmission risks.

The singer's four-year-old son has had trouble with his speech development.

The Trump campaign released a new ad in Spanish targeting Democrats for their response to the allegations Tara Reade made against presumptive Democratic nominee Joe Biden.

Tara Reade has claimed in a new interview that Joe Biden used graphic language to proposition her for sex when he allegedly assaulted her in 1993.

Newly released FBI documents suggest a federal judge in Washington was swayed by apparent misrepresentations from a top prosecutor when he issued a memorandum opinion in December 2019 -- a ruling that marked one of the lowest points in former U.S. national security adviser Michael Flynn's effort to withdraw a guilty plea on a charge of lying to investigators.

Vanessa Bryant, the widow of late NBA star Kobe Bryant, filed a legal claim Friday against the Los Angeles County Sheriff’s Department over graphic photos that deputies allegedly took and shared of the helicopter crash scene where her husband, 13-year-old daughter, Gigi, and seven others were killed in late January.

Read our live updates on coronavirus HERE Coronavirus: The symptoms

The PM was moved out of intensive care on Thursday evening

Follow our live coronavirus updates here Coronavirus: the symptoms

Read our live coronavirus updates HERE Coronavirus: The symptoms

Covid-19: The symptoms Read our live blogs for updates here

Follow our live Covid-19 updates HERE Coronavirus: The symptoms

Read our live coronavirus updates HERE

Coronavirus: the symptoms Read our LIVE updates on the coronavirus here

Follow our live coronavirus updates here Coronavirus: the symptoms

Coronavirus: the symptoms Read our LIVE updates on the coronavirus here

Read our live updates on coronavirus HERE Coronavirus: The symptoms

Follow our live Covid-19 updates HERE

Read our live coronavirus updates HERE Coronavirus: The symptoms

Nicholas Hazell, 36, from Epsom, Surrey, assaulted an officer in a hospital after being taken there when he falsely claimed to have swallowed drugs.

A single UK ticket-holder has staked a claim for the £57.8 million EuroMillions jackpot.

Follow our live coronavirus updates HERE Coronavirus: The symptoms

A motorcyclist has died after a crash on a busy road in north-east London.

MPs are calling for 'strategic clarity' from the Government

Former Home Office Permanent Secretary Sir Philip Rutnam has formally begun legal action against Home Secretary Priti Patel, claiming "constructive dismissal".

Here are the Government guidelines on how the furlough scheme portal works