After 11 seasons and more than 80 episodes, it looks like funny man and avid car nut Jerry Seinfeld's "Comedians In Cars Getting Coffee" is finally running out of gas. During a press conference to discuss his new Netflix special “Jerry Seinfeld: 23 Hours to Kill,” Seinfeld said he feels like there's nothing left to achieve with...

This week Constellation, an Exelon company, said it signed a long-term PPA with Starbucks to power more than 340 Illinois stores with 100 percent renewable energy produced by Enel Green Power (EGP) North America, Inc.’s HillTopper wind project in Logan, County, Ill.

A European marine energy project program under the new Ocean Energy ERA-Net Cofund (Oceanera-NET Cofund) is proceeding with stage two, Cofund Joint Call 2017, on March 20.  

Baristas normally aim to grind coffee finely to maximise surface area and extract the most coffee compounds, but a mathematical analysis has found that coarse grounds are better as they reduce clogging

Genetic analysis suggests all Arabica coffee plants are descended from a single common ancestor, and this lack of genetic diversity makes them vulnerable to extinction

Romans flocked to the city's bars and cafes for their caffeine fix on Tuesday - but only takeaway options are permitted.

Coffee has been linked to changes on our DNA that affect how active certain genes are. The finding may help explain some of coffee's touted health benefits

Title: Coffee, Wine Good for Healthy Gut, Sodas May Be Bad
Category: Health News
Created: 4/28/2016 12:00:00 AM
Last Editorial Review: 4/29/2016 12:00:00 AM

Title: Taking the Stairs a Better Pick-Me-Up Than Coffee
Category: Health News
Created: 4/28/2017 12:00:00 AM
Last Editorial Review: 5/1/2017 12:00:00 AM

Title: Drink Up! Coffee Won't Harm Your Heart, Study Finds
Category: Health News
Created: 5/7/2020 12:00:00 AM
Last Editorial Review: 5/8/2020 12:00:00 AM

Photorespiration is an essential process in oxygenic photosynthetic organisms triggered by the oxygenase activity of Rubisco. In peroxisomes, photorespiratory HYDROXYPYRUVATE REDUCTASE1 (HPR1) catalyzes the conversion of hydroxypyruvate to glycerate together with the oxidation of a pyridine nucleotide cofactor. HPR1 regulation remains poorly understood; however, HPR1 phosphorylation at T335 has been reported. By comparing the kinetic properties of phosphomimetic (T335D), nonphosphorylatable (T335A), and wild-type recombinant Arabidopsis (Arabidopsis thaliana) HPR1, it was found that HPR1-T335D exhibits reduced NADH-dependent hydroxypyruvate reductase activity while showing improved NADPH-dependent activity. Complementation of the Arabidopsis hpr1-1 mutant by either wild-type HPR1 or HPR1-T335A fully complemented the photorespiratory growth phenotype of hpr1-1 in ambient air, whereas HPR1-T335D-containing hpr1-1 plants remained smaller and had lower photosynthetic CO₂ assimilation rates. Metabolite analyses indicated that these phenotypes were associated with subtle perturbations in the photorespiratory cycle of HPR1-T335D-complemented hpr1-1 rosettes compared to all other HPR1-containing lines. Therefore, T335 phosphorylation may play a role in the regulation of HPR1 activity in planta, although it was not required for growth under ambient air controlled conditions. Furthermore, improved NADP-dependent HPR1 activities in peroxisomes could not compensate for the reduced NADH-dependent HPR1 activity.

The TEAD family of transcription factors requires associating cofactors to induce gene expression. TEAD1 is known to activate the early promoter of human papillomavirus (HPV), but the precise mechanisms of TEAD1-mediated transactivation of the HPV promoter, including its relevant cofactors, remain unexplored. Here, we reveal that VGLL1, a TEAD-interacting cofactor, contributes to HPV early gene expression. Knockdown of VGLL1 and/or TEAD1 led to a decrease in viral early gene expression in human cervical keratinocytes and cervical cancer cell lines. We identified 11 TEAD1 target sites in the HPV16 long control region (LCR) by in vitro DNA pulldown assays; 8 of these sites contributed to the transcriptional activation of the early promoter in luciferase reporter assays. VGLL1 bound to the HPV16 LCR via its interaction with TEAD1 both in vitro and in vivo. Furthermore, introducing HPV16 and HPV18 whole genomes into primary human keratinocytes led to increased levels of VGLL1, due in part to the upregulation of TEADs. These results suggest that multiple VGLL1/TEAD1 complexes are recruited to the LCR to support the efficient transcription of HPV early genes.

IMPORTANCE Although a number of transcription factors have been reported to be involved in HPV gene expression, little is known about the cofactors that support HPV transcription. In this study, we demonstrate that the transcriptional cofactor VGLL1 plays a prominent role in HPV early gene expression, dependent on its association with the transcription factor TEAD1. Whereas TEAD1 is ubiquitously expressed in a variety of tissues, VGLL1 displays tissue-specific expression and is implicated in the development and differentiation of epithelial lineage tissues, where HPV gene expression occurs. Our results suggest that VGLL1 may contribute to the epithelial specificity of HPV gene expression, providing new insights into the mechanisms that regulate HPV infection. Further, VGLL1 is also critical for the growth of cervical cancer cells and may represent a novel therapeutic target for HPV-associated cancers.

Sriram Sudarsanam, Shiri Yaniv, Hagar Meltzer, and Oren Schuldiner

The mechanisms that control intrinsic axon growth potential, and thus axon regeneration following injury, are not well understood. Developmental axon regrowth of Drosophila mushroom body -neurons during neuronal remodeling offers a unique opportunity to study the molecular mechanisms controlling intrinsic growth potential. Motivated by the recently uncovered developmental expression atlas of -neurons, we here focus on the role of the actin-severing protein cofilin during axon regrowth. We show that Twinstar (Tsr), the fly cofilin, is a crucial regulator of both axon growth and branching during developmental remodeling of -neurons. tsr mutant axons demonstrate growth defects both in vivo and in vitro, and also exhibit actin-rich filopodial-like structures at failed branch points in vivo. Our data is inconsistent with Tsr being important for increasing G-actin availability. Furthermore, analysis of microtubule localization suggests that Tsr is required for microtubule infiltration into the axon tips and branch points. Taken together, we show that Tsr promotes axon growth and branching, likely by clearing F-actin to facilitate protrusion of microtubules.

Your coffee may be fair trade, but is it locally sourced? Who did the grinding of those beans? It’s been alleged that your Aeropress had...

Coffee has been linked to changes on our DNA that affect how active certain genes are. The finding may help explain some of coffee's touted health benefits

On the criteria for a claimant to be looking for work being paused for three months, Ms Coffey said: "We do however want claimants to continue to look for work wherever they are able to do so."

A Colombian advertising company is pitching a novel if morbid solution to shortages of hospital beds and coffins during the coronavirus pandemic: combine them. ABC Displays has created a cardboard bed with metal railings that designers say can double as a casket if a patient dies. Company manager Rodolfo Gómez said he was inspired to find a way to help after watching events unfold recently in nearby Ecuador.

So far, four of the show's recurring characters have teamed up with fundraising site Omaze for the event

Channel 4 star came to fame alongside her husband Leon, who died in 2017

Judge Alison J. Nathan has ruled that the 66-year-old comedian is the creator of the show.

June Bernicoff passed away on May 5 following a short illness, her husband, Leon died in December 2017 and June never appeared on the show again

Gogglebox paid tribute to one of its best-loved stars, June Bernicoff, who died earlier this week, bringing viewers to tears

June and her husband Leon were favourites on the show.

Moderate, daily coffee consumption does not trigger incident heart arrhythmias, according to an analysis of prospectively collected data from nearly 300,000 residents of the United Kingdom.
Medscape Medical News

It is one of the most popular and important websites for cattle producers, rangers and carbon farmers in northern Australia, but its funding is about to run out.

Disposable cups are currently the only option for most cafe owners to provide takeaway coffee to customers, but environmentalists are worried about the consequences.

IF you buy a large cappuccino you may find it is quite cold by the time you get to the bottom. Thankfully, there is now a solution to this.

Gogglebox star June Bernicoff has died at the age of 82, according to Channel 4. A...

A Colombian advertising company is pitching a novel if morbid solution to shortages of hospital beds and coffins during the coronavirus pandemic: combine them.ABC Displays has created a cardboard bed with metal railings that designers...

Gogglebox star June Bernicoff has died at the age of 82 after a short illness.

Indonesia's government dithered for nearly two months before enacting social restrictions. Tens of thousands could die of COVID-19.

June and her late husband Leon were among the original stars of the Channel Four reality show

Manuel Rodriguez, 47, of Lafayette, La., was sentenced today to 120 months in prison and three years supervised release for committing fraud in connection with a coffee machine business opportunity scheme.

We hate pods, but love durability. Is there a place for this?

In the remote villages around the Cheng Long Wetlands in Taiwan, engagement with environmental issues is on the rise thanks in part to a community-based program that is turning the wetlands into a 'gallery' for ecofriendly art.

Customers pay €1 for a reusable cup that can be returned to any participating business in the city center.

Chocolate, tea and more goodies partner with Fair Trade USA which expands farming programs and experiences record sales.

These pods of evil have taken over North America, bringing tons of waste with them.

Whether it's coffee or tea, Katherine and Margaret love their steaming mugs of deliciousness.

Take an average cup of coffee or tea to a new tasty level with these quick, easy additions.

Not only is coffee leaf tea delicious and nutritious, but it also offers a more stable source of income for coffee growers in Latin America.

What's it going to be? Check out this fun infographic to learn amusing and interesting facts about both popular beverages.

These popular hot beverages inhabit places of honor in our homes, apparently for very good reasons.

With Starbucks poised to open its first store on Italian soil this fall, there is concern about the environmental impact of so much coffee-related trash.

The same inventor who brought us the body heat-powered flashlight is at it again.

Don't take your morning cup of coffee for granted. It may not be around in a few decades, according to a new report.

And a few looks back at themes of the last ten years.