Galloway, J M; Dewing, K; Piepjohn, K; Smith, I R. Geological Survey of Canada, Open File 8750, 2020, 44 pages, https://doi.org/10.4095/327426
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/gid_327426.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/gid_327426.jpg" title="Geological Survey of Canada, Open File 8750, 2020, 44 pages, https://doi.org/10.4095/327426" height="150" border="1" /></a>

Rice, J M; Paulen, R C; Ross, M; McClenaghan, M B. Geological Survey of Canada, Open File 8721, 2020, 14 pages, https://doi.org/10.4095/326083
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/gid_326083.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/gid_326083.jpg" title="Geological Survey of Canada, Open File 8721, 2020, 14 pages, https://doi.org/10.4095/326083" height="150" border="1" /></a>

Dafoe, L T; Williams, G L. Geological Survey of Canada, Open File 8702, 2020, 41 pages, https://doi.org/10.4095/321758
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/of8702.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/of8702.jpg" title="Geological Survey of Canada, Open File 8702, 2020, 41 pages, https://doi.org/10.4095/321758" height="150" border="1" /></a>

Rice, J M; McClenaghan, M B; Paulen, R C; Pyne, M D; Ross, M; Campbell, H E. Geological Survey of Canada, Open File 8655, 2020, 11 pages, https://doi.org/10.4095/321471
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/of8655.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/of8655.jpg" title="Geological Survey of Canada, Open File 8655, 2020, 11 pages, https://doi.org/10.4095/321471" height="150" border="1" /></a>

Plakholm, J A; Paulen, R C; Paré, D. Geological Survey of Canada, Open File 8437, 2020, 17 pages, https://doi.org/10.4095/321451
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/of8437.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/of8437.jpg" title="Geological Survey of Canada, Open File 8437, 2020, 17 pages, https://doi.org/10.4095/321451" height="150" border="1" /></a>

Smith, I R; Day, S J A; Paulen, R C; Pearson, D G. Geological Survey of Canada, Open File 8799, 2021, 29 pages, https://doi.org/10.4095/329080
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/gid_329080.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/gid_329080.jpg" title="Geological Survey of Canada, Open File 8799, 2021, 29 pages, https://doi.org/10.4095/329080" height="150" border="1" /></a>

Bingham-Koslowski, N; Miller, M A. Geological Survey of Canada, Open File 8819, 2021, 7 pages, https://doi.org/10.4095/328813
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/gid_328813.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/gid_328813.jpg" title="Geological Survey of Canada, Open File 8819, 2021, 7 pages, https://doi.org/10.4095/328813" height="150" border="1" /></a>

Davis, W J. Geological Survey of Canada, Open File 8807, 2021, 6 pages, https://doi.org/10.4095/328453
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/gid_328453.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/gid_328453.jpg" title="Geological Survey of Canada, Open File 8807, 2021, 6 pages, https://doi.org/10.4095/328453" height="150" border="1" /></a>

Galloway, J M; Bringué, M; Buryak, S D; Reyes, A V; West, C K; Siver, P A. Geological Survey of Canada, Open File 8864, 2022, 29 pages, https://doi.org/10.4095/329615
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/gid_329615.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/gid_329615.jpg" title="Geological Survey of Canada, Open File 8864, 2022, 29 pages, https://doi.org/10.4095/329615" height="150" border="1" /></a>

Posted on Tuesday, March 24, 2015

Arlington, VA – The American Trucking Associations is calling for the federal government to release guidelines and standards regarding the use of hair samples as a drug-testing method for commercial motor vehicle drivers.

Preparation of biomacromolecules for structural biology studies is a complex and time-consuming process. The goal is to produce a highly concentrated, highly pure product that is often shipped to large facilities with tools to prepare the samples for crystallization trials or for measurements at synchrotrons and cryoEM centers. The aim of this article is to provide guidance and to discuss general considerations for shipping biomacromolecular samples. Details are also provided about shipping samples for specific experiment types, including solution- and cryogenic-based techniques. These guidelines are provided with the hope that the time and energy invested in sample preparation is not lost due to shipping logistics.

Despite the increased brilliance of the new generation synchrotron sources, there is still a challenge with high-resolution scanning of very thick and absorbing samples, such as a whole mouse brain stained with heavy elements, and, extending further, brains of primates. Samples are typically cut into smaller parts, to ensure a sufficient X-ray transmission, and scanned separately. Compared with the standard tomography setup where the sample would be cut into many pillars, the laminographic geometry operates with slab-shaped sections significantly reducing the number of sample parts to be prepared, the cutting damage and data stitching problems. In this work, a laminography pipeline for imaging large samples (>1 cm) at micrometre resolution is presented. The implementation includes a low-cost instrument setup installed at the 2-BM micro-CT beamline of the Advanced Photon Source. Additionally, sample mounting, scanning techniques, data stitching procedures, a fast reconstruction algorithm with low computational complexity, and accelerated reconstruction on multi-GPU systems for processing large-scale datasets are presented. The applicability of the whole laminography pipeline was demonstrated by imaging four sequential slabs throughout an entire mouse brain sample stained with osmium, in total generating approximately 12 TB of raw data for reconstruction.

For cryo-electron tomography (cryo-ET) of beam-sensitive biological specimens, a planar sample geometry is typically used. As the sample is tilted, the effective thickness of the sample along the direction of the electron beam increases and the signal-to-noise ratio concomitantly decreases, limiting the transfer of information at high tilt angles. In addition, the tilt range where data can be collected is limited by a combination of various sample-environment constraints, including the limited space in the objective lens pole piece and the possible use of fixed conductive braids to cool the specimen. Consequently, most tilt series are limited to a maximum of ±70°, leading to the presence of a missing wedge in Fourier space. The acquisition of cryo-ET data without a missing wedge, for example using a cylindrical sample geometry, is hence attractive for volumetric analysis of low-symmetry structures such as organelles or vesicles, lysis events, pore formation or filaments for which the missing information cannot be compensated by averaging techniques. Irrespective of the geometry, electron-beam damage to the specimen is an issue and the first images acquired will transfer more high-resolution information than those acquired last. There is also an inherent trade-off between higher sampling in Fourier space and avoiding beam damage to the sample. Finally, the necessity of using a sufficient electron fluence to align the tilt images means that this fluence needs to be fractionated across a small number of images; therefore, the order of data acquisition is also a factor to consider. Here, an n-helix tilt scheme is described and simulated which uses overlapping and interleaved tilt series to maximize the use of a pillar geometry, allowing the entire pillar volume to be reconstructed as a single unit. Three related tilt schemes are also evaluated that extend the continuous and classic dose-symmetric tilt schemes for cryo-ET to pillar samples to enable the collection of isotropic information across all spatial frequencies. A fourfold dose-symmetric scheme is proposed which provides a practical compromise between uniform information transfer and complexity of data acquisition.

In addressing the challenges faced by laboratories and universities with limited (or no) cryo-electron microscopy (cryo-EM) infrastructure, the ESRF, in collaboration with the Grenoble Institute for Structural Biology (IBS), has implemented the cryo-EM Solution-to-Structure (SOS) pipeline. This inclusive process, spanning grid preparation to high-resolution data collection, covers single-particle analysis and cryo-electron tomography (cryo-ET). Accessible through a rolling access route, proposals undergo scientific merit and technical feasibility evaluations. Stringent feasibility criteria demand robust evidence of sample homogeneity. Two distinct entry points are offered: users can either submit purified protein samples for comprehensive processing or initiate the pipeline with already vitrified cryo-EM grids. The SOS pipeline integrates negative stain imaging (exclusive to protein samples) as a first quality step, followed by cryo-EM grid preparation, grid screening and preliminary data collection for single-particle analysis, or only the first two steps for cryo-ET. In both cases, if the screening steps are successfully completed, high-resolution data collection will be carried out using a Titan Krios microscope equipped with a latest-generation direct electron counting detector coupled to an energy filter. The SOS pipeline thus emerges as a comprehensive and efficient solution, further democratizing access to cryo-EM research.

X-ray Laue microdiffraction aims to characterize microstructural and mechanical fields in polycrystalline specimens at the sub-micrometre scale with a strain resolution of ∼10−4. Here, a new and unique Laue microdiffraction setup and alignment procedure is presented, allowing measurements at temperatures as high as 1500 K, with the objective to extend the technique for the study of crystalline phase transitions and associated strain-field evolution that occur at high temperatures. A method is provided to measure the real temperature encountered by the specimen, which can be critical for precise phase-transition studies, as well as a strategy to calibrate the setup geometry to account for the sample and furnace dilation using a standard α-alumina single crystal. A first application to phase transitions in a polycrystalline specimen of pure zirconia is provided as an illustrative example.

Mass spectrometry-based proteomics data from thousands of HeLa control samples | Scientific Data  Nature.com

Repurposed 3D Printer Allows Economical and Programmable Fraction Collection for Proteomics of Nanogram Scale Samples  ACS Publications

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New Annex A on guidelines on selection of representative samples

We have added presentation sample design ideas for you to use in businesses and corporate offices including display graphs for a e-commerce warehouse distribution and electrical distribution companies.

The Department of Natural Resources and Environmental Control released findings from DNREC’s comprehensive study on PFAS (per- and polyfluoroalkyl substances) in surface waters across Delaware.

Title: China Withholding H7N9 Bird Flu Virus Samples From U.S.
Category: Health News
Created: 8/31/2018 12:00:00 AM
Last Editorial Review: 8/31/2018 12:00:00 AM

The human major histocompatibility complex (MHC) is a ~4 Mb genomic segment on Chromosome 6 that plays a pivotal role in the immune response. Despite its importance in various traits and diseases, its complex nature makes it challenging to accurately characterize on a routine basis. We present a novel approach allowing targeted sequencing and de novo haplotypic assembly of the MHC region in heterozygous samples, using long-read sequencing technologies. Our approach is validated using two reference samples, two family trios, and an African-American sample. We achieved excellent coverage (96.6%–99.9% with at least 30x depth) and high accuracy (99.89%–99.99%) for the different haplotypes. This methodology offers a reliable and cost-effective method for sequencing and fully characterizing the MHC without the need for whole-genome sequencing, facilitating broader studies on this important genomic segment and having significant implications in immunology, genetics, and medicine.

Novel device developed can be clipped onto a smartphone to rapidly test for Zika virus in a single droplet of blood, reveal researchers at the University of Illinois Urbana-Champaign.

Four spurious drugs were also found and action has been initiated against the makers, the DCGI said