Hoy comparto contigo información sobre una iniciativa de Fundación Occident: las becas de grado Jesús Serra, que se convocan por primera vez en 2024. Además […]

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Ilham Aliyev critique l'occident à l'ouverture de la COP29

Words and tools are powerful, but are they our only way of making a mark? *Pause for emphasis* Stephan Moccio doesn’t think so. An artist, composer, and producer, he’s the name behind some of the most well-known songs and soundtracks on the planet. But surprisingly enough, all that success with sound has come through silence. In this podcast, I have the privilege of sitting down with him to discuss how strategic refrain and thought can benefit creative power. Creativity as a Muscle Skill can come naturally, but people rarely get to the top through their natural-born talents alone. Even 6’9″ LeBron James wouldn’t be where he is as a basketball player without putting in the unseen hours of success. Whether physical or mental, training effectively determines performance. “I’ve learned that to get great things and to achieve great things requires a lot of work behind the curtain…” Creative practice isn’t different from other forms of training in that making a habit out of it is hard. We all have lives outside of our passions, and those passions aren’t always fully funded. Many of us work day jobs while pursuing creative endeavors. Stephan has had to balance paying bills, raising a...

The post Stephan Moccio: The Power of Pauses in Life (and Music) first appeared on Chase Jarvis.

The post Stephan Moccio: The Power of Pauses in Life (and Music) appeared first on Chase Jarvis.

Desde el inicio de la pandemia del COVID-19, los productores agrícolas de Guatemala han afrontado múltiples restricciones de movimiento tanto locales como nacionales, así como también disrupciones en las cadenas de valor agrícolas. Asimismo, los productores han estado expuestos a varios choques externos como las tormentas tropicales de ETA e IOTA hacia finales de 2020 y el reciente conflicto bélico en Europa del Este y crisis de precios.

El director mexicano estrena 'La cocina', un exuberante retrato en clave musical de la parte de atrás de la emigración legal en Estados Unidos Leer

'El corazón del naga', la primera parte de la tetralogía 'El pájaro que bebe lágrimas', aterriza en España y otros 12 países Leer

El alcalde de Cali, Alejandro Eder, se refirió en 6AM al llamado de los mandatarios del suroccidente para  enfrentar la grave situación de orden público y los recurrentes bloqueos de la vía Panamericana

Despite its major importance in human health, the metabolic potential of the human gut microbiota is still poorly understood. We have recently shown that biosynthesis of Ruminococcin C (RumC), a novel ribosomally synthesized and posttranslationally modified peptide (RiPP) produced by the commensal bacterium Ruminococcus gnavus, requires two radical SAM enzymes (RumMC1 and RumMC2) catalyzing the formation of four Cα-thioether bridges. These bridges, which are essential for RumC's antibiotic properties against human pathogens such as Clostridium perfringens, define two hairpin domains giving this sactipeptide (sulfur-to-α-carbon thioether–containing peptide) an unusual architecture among natural products. We report here the biochemical and spectroscopic characterizations of RumMC2. EPR spectroscopy and mutagenesis data support that RumMC2 is a member of the large family of SPASM domain radical SAM enzymes characterized by the presence of three [4Fe-4S] clusters. We also demonstrate that this enzyme initiates its reaction by Cα H-atom abstraction and is able to catalyze the formation of nonnatural thioether bonds in engineered peptide substrates. Unexpectedly, our data support the formation of a ketoimine rather than an α,β-dehydro-amino acid intermediate during Cα-thioether bridge LC–MS/MS fragmentation. Finally, we explored the roles of the leader peptide and of the RiPP precursor peptide recognition element, present in myriad RiPP-modifying enzymes. Collectively, our data support a more complex role for the peptide recognition element and the core peptide for the installation of posttranslational modifications in RiPPs than previously anticipated and suggest a possible reaction intermediate for thioether bond formation.

Berlin ; Boston : De Gruyter, [2023]

México, D.F. : Escuela Nacional de Antropología e Historia, 1981.

Bilateral occipital nerve release surgery was the first, significant step to relieving Scurry's debilitating post-concussive headaches.

(United States Second Circuit) - Questions on appeal concern cross-jurisdictional tolling of a class action. Because the appeal presents state law questions that New York’s courts have yet to address, the court certifies the case to the New York Court of Appeals.

(United States Second Circuit) - Questions on appeal concern cross-jurisdictional tolling of a class action. Because the appeal presents state law questions that New York’s courts have yet to address, the court certifies the case to the New York Court of Appeals.

Berlin : A. Hirschwald, 1892.

Gregor Thut
Sep 13, 2006; 26:9494-9502
BehavioralSystemsCognitive

ABSTRACT

Streptococcus pneumoniae (or pneumococcus) is a highly prevalent human pathogen. Toll-like receptors (TLRs) function as immune sensors that can trigger host defenses against this bacterium. Defects in TLR-activated signaling pathways, including deficiency in the adaptor protein myeloid differentiation factor 88 (MyD88), are associated with markedly increased susceptibility to infection. However, the individual MyD88-dependent TLRs predominantly involved in antipneumococcal defenses have not been identified yet. Here we find that triple knockout mice simultaneously lacking TLR7, TLR9, and TLR13, which sense the presence of bacterial DNA (TLR9) and RNA (TLR7 and TLR13) in the phagolysosomes of phagocytic cells, display a phenotype that largely resembles that of MyD88-deficient mice and rapidly succumb to pneumococcal pneumonitis due to defective neutrophil influx into the lung. Accordingly, TLR7/9/13 triple knockout resident alveolar macrophages were largely unable to respond to pneumococci with the production of neutrophil-attracting chemokines and cytokines. Mice with single deficiencies of TLR7, TLR9, or TLR13 showed unaltered ability to control lung infection but were moderately more susceptible to encephalitis, in association with a decreased ability of microglia to mount cytokine responses in vitro. Our data point to a dominant, tissue-specific role of nucleic acid-sensing pathways in innate immune recognition of S. pneumoniae and also show that endosomal TLRs are largely capable of compensating for the absence of each other, which seems crucial to prevent pneumococci from escaping immune recognition. These results may be useful to develop novel strategies to treat infections by antibiotic-resistant pneumococci based on stimulation of the innate immune system.

IMPORTANCE The pneumococcus is a bacterium that frequently causes infections in the lungs, ears, sinus cavities, and meninges. During these infections, body defenses are triggered by tissue-resident cells that use specialized receptors, such as Toll-like receptors (TLRs), to sense the presence of bacteria. We show here that pneumococci are predominantly detected by TLRs that are located inside intracellular vacuoles, including endosomes, where these receptors can sense the presence of nucleic acids released from ingested bacteria. Mice that simultaneously lacked three of these receptors (specifically, TLR7, TLR9, and TLR13) were extremely susceptible to lung infection and rapidly died after inhalation of pneumococci. Moreover, tissue-resident macrophages from these mice were impaired in their ability to respond to the presence of pneumococci by producing inflammatory mediators capable of recruiting polymorphonuclear leucocytes to infection sites. This information may be useful to develop drugs to treat pneumococcal infections, particularly those caused by antibiotic-resistant strains.

Tedizolid (TZD) and daptomycin (DAP) were assessed in a rat endocarditis model against Enterococcus faecalis, Enterococcus faecium (resistant to vancomycin and ampicillin), and Staphylococcus aureus. As a monotherapy, TZD for 5 days was not effective in a comparison with no-treatment controls, while DAP for 5 days was significantly effective against these bacteria. Step-down therapy (DAP for 3 days followed by TZD for 2 days) was as effective as DAP for 5 days and was comparable to 3 days of DAP plus ceftriaxone against all bacteria and to 3 days of DAP plus gentamicin against E. faecalis OG1RF.

Enterococcus faecium strains are commonly resistant to vancomycin and β-lactams. In addition, E. faecium often causes biofilm-associated infections and these infections are difficult to treat. In this context, we investigated the activity of dosing regimens using daptomycin (DAP) (8, 10, 12, and 14 mg/kg of body weight/day) alone and in combination with ceftaroline (CPT), ampicillin (AMP), ertapenem (ERT), and rifampin (RIF) against 2 clinical strains of biofilm-producing vancomycin-resistant Enterococcus faecium (VREfm), namely, strains S447 and HOU503, in an in vitro biofilm model. HOU503 harbors common LiaS and LiaR substitutions, whereas S447 lacks mutations associated with the LiaFSR pathway. MIC results demonstrated that both strains were susceptible to DAP and resistant to CPT, AMP, ERT, and RIF. The 168-h pharmacokinetic/pharmacodynamic (PK/PD) CDC biofilm reactor models (simulating human antibiotic exposures) were used with titanium and polyurethane coupons to evaluate the efficacy of antibiotic combinations. DAP 12 and 14 achieved bactericidal activity against S447 but lacked such effect against HOU503. Addition of ERT and RIF enhanced DAP activity, allowing DAP 8 and 10 plus ERT or RIF to produce bactericidal activity against both strains at 168 h. While DAP 8 and 10 plus CPT improved killing, they did not reach bactericidal reduction against S447. Combination of AMP, CPT, ERT, or RIF resulted in enhanced and bactericidal activity for DAP against HOU503 at 168 h. Our data provide further support for the use of combinations of DAP with AMP, ERT, CPT, and RIF in infections caused by biofilm producing VREfm. Further research involving DAP combinations against biofilm-producing enterococci is warranted.

Occidental Petroleum Corporation, Occidental Oil and Gas Corporation, and OXY USA Inc. have agreed to pay the United States $2.05 million plus interest to resolve claims that the companies violated the False Claims Act by knowingly underpaying royalties owed on natural gas produced from federal leases.

Activist investor has almost quadrupled his stake in battered US oil producer

Barker Library - TA357.5.T87 L367 2012

Barker Library - TA357.5.T87 L38 2014

Online Resource

Gouhier, Henri, 1898-1994, author

Barker Library - B105.P535 C6313 2019

Rotch Library - NA388.A73 2006

Rotch Library - NA2765.V34 2017

Rotch Library - NA1111.L37 2018