living cells

Chemically engineered exogenous organic reactions in living cells for in situ fluorescence imaging and biomedical applications

J. Mater. Chem. B, 2024, Advance Article
DOI: 10.1039/D4TB01925C, Review Article
Gang Song, Zhiwen Yang, Yiming Huang, Haotian Bai, Fengting Lv, Shu Wang
Key intracellular in situ synthesis processes, including the synthesis of near-infrared fluorescent dyes, intracellular oxidative cross-linking, polymerization, and bioorthogonal reactions, as well as their biomedical applications were summarized.
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living cells

Phenoxy-1,2-dioxetane-Based Activatable Chemiluminescent Probes: Tuning of Photophysical Properties for Tracing Enzymatic Activities in Living Cells

Analyst, 2024, Accepted Manuscript
DOI: 10.1039/D4AN01082E, Critical Review
Open Access
Jagpreet Singh Sidhu, Gurjot Kaur, Atharva Rajesh Chavan, Mandeep Chahal, Rajeev Taliyan
The use of chemiluminophores for tracing enzymatic activities in live-cell imaging has gained significant attention, making them valuable tools for diagnostic applications. Among various chemiluminophores, the phenoxy-1,2-dioxetane scaffold exhibits significant...
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living cells

Imaging specific proteins in living cells with small unnatural amino acid attached Raman reporters

Analyst, 2024, 149,5476-5481
DOI: 10.1039/D4AN00758A, Paper
Erli Cai, Yage Chen, Jing Zhang, Haozheng Li, Yiran Li, Shuai Yan, Zhiyong He, Quan Yuan, Ping Wang
For living HeLa cells, we report a small and minimally-invasive Raman reporter (about 2 aa and <1 kDa), which can be site-specifically introduced into proteins by genetic codon expansion combined with tetrazine ligation.
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living cells

New technique uses ultrasound to align living cells in 3D bioprinted tissues

One of the ways of making bioengineered tissues is to embed living cells within 3D printed structures. But one challenge has been organizing the cells in 3D bioprinted gels, so that the engineered tissue more closely mimics natural tissues.



  • 3D Printing Applications

living cells

[ASAP] CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells

Biochemistry
DOI: 10.1021/acs.biochem.0c00281




living cells

A Novel Strategy of Rhodamine B-based Fluorescent Probe for Selective Response of Glutathione to Serve as Bioimaging in Living Cells

Analyst, 2020, Accepted Manuscript
DOI: 10.1039/D0AN00582G, Paper
Zhipeng Li, Wei Xiong, Xiaojun He, Xiaoliang Qi, Feng Ding, Jianliang Shen
To overcome the reported shortcomings of the glutathione (GSH) detection of rhodamine based fluorescent probes, such as poor selectivity to thiol groups and reversible unstable covalent binding with the thiol...
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living cells

FRET based fluorescent ratiometric probes for rapid detection of endogenous hydrogen sulphide in living cells

Analyst, 2020, Accepted Manuscript
DOI: 10.1039/D0AN00531B, Paper
Yuming Zhang, Yuncong Chen, Bai Yang, Xueling Xue, Weijiang He, Zijian Guo
Real time monitor of hydrogen sulphide (H2S) level change is of crucial importance for the study of its complicated roles in physiology. Herein, we developed a FRET strategy for designing...
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living cells

Phenyl doped graphitic carbon nitride nanosheets for sensing of copper ions in living cells

Analyst, 2020, Accepted Manuscript
DOI: 10.1039/D0AN00795A, Paper
Zhiping Song, Yuanteng Xu, Liangqia Guo
Copper (Cu) is a vital mental element for human and animals. Monitoring and evaluating the concentration level of Cu2+ in biological body is an effective way to prevent a variety...
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living cells

[ASAP] In Situ Study of the Drug–Target Protein Interaction in Single Living Cells by Combining Fluorescence Correlation Spectroscopy with Affinity Probes

Analytical Chemistry
DOI: 10.1021/acs.analchem.0c00263




living cells

HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues

Org. Chem. Front., 2020, 7,993-996
DOI: 10.1039/D0QO00081G, Research Article
Xiaoyu Bai, Bowei Yang, Hansen Chen, Jiangang Shen, Dan Yang
Highly sensitive and selective yellow probes, HKOCl-4 and its derivatives, have been developed for detecting endogenous HOCl in cytosol and mitochondria of living cells. In addition, visualization of HOCl production in ischemic stroke model has been achieved with HKOCl-4r.
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living cells

[ASAP] Correction to “Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells”

ACS Sensors
DOI: 10.1021/acssensors.0c00744




living cells

Non-invasive manipulation of membrane potential in intact living cells