ESET researchers have discovered threats abusing the success of the Hamster Kombat clicker game

Margex Exchange announced new airdrop

Margex extends Hamster Combat airdrop

"Human vs. Hamster," a very real reality show, premieres November 21 on Max.

Chris confesses to being a French Karen. Kelley is consumed by the Lego mimic freebie. And Robert gets an anime pretzel robot roommate.

The post RPG Cast – Episode 718: “Hamsterbam” appeared first on RPGamer.

Watch a hungry hamster and his furry friends devour a holiday feast made of miniature food.

Simultaneously burn calories, cut back on your utility bills and do the laundry with a conceptual washing machine-treadmill hybrid dubbed Wheel.

25-year-old wave runner Lindsey Russell undertook the 20-mile challenge to raise money for charity.

Pellagra, which is caused by a deficiency in vitamin B3 (niacin), showing up in hamsters because of large-scale monoculture.

Mice, rats, or hamsters are immunized by giving biweekly injections of a purified antigen, cultured cells, or cDNA. For mice, if a pure, soluble protein antigen is being used and is abundant, a dose of 50–100 µg in adjuvant at each immunization is a sensible general recommendation; for rats and hamsters, a dose of 100–200 µg is sufficient. Lower doses can be used for antigens with higher immunogenicity. Adjuvants (Freund's, Ribi, Hunter's TiterMax, ImmunEasy, or Alum) should be mixed with the immunizing antigen for the first two immunizations only; Complete Freund's adjuvant is only used with the first immunization. Subsequent immunizations are performed in phosphate-buffered saline (PBS) or normal saline, with or without Incomplete Freund's adjuvant. The choice of adjuvant is dependent on the subclass of immunoglobulin required. Over the course of the 6-wk immunization schedule, each animal usually receives a total of six injections (three subcutaneous and three intraperitoneal). Once a good titer has developed against the antigen of interest, regular boosts and bleeds are performed to collect the maximum amount of serum. For rats and hamsters, boosts should be spaced every 2–3 wk, and serum samples of 400–500 µL should be collected 10–12 d after each boost. For mice, boosts should be spaced every 2–3 wk, and serum samples of 200–300 µL should be collected 10–12 d after each boost.

ABSTRACT

Hantaviruses are the etiological agent of hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). The latter is associated with case fatality rates ranging from 30% to 50%. HCPS cases are rare, with approximately 300 recorded annually in the Americas. Recently, an HCPS outbreak of unprecedented size has been occurring in and around Epuyén, in the southwestern Argentinian state of Chubut. Since November of 2018, at least 29 cases have been laboratory confirmed, and human-to-human transmission is suspected. Despite posing a significant threat to public health, no treatment or vaccine is available for hantaviral disease. Here, we describe an effort to identify, characterize, and develop neutralizing and protective antibodies against the glycoprotein complex (Gn and Gc) of Andes virus (ANDV), the causative agent of the Epuyén outbreak. Using murine hybridoma technology, we generated 19 distinct monoclonal antibodies (MAbs) against ANDV GnGc. When tested for neutralization against a recombinant vesicular stomatitis virus expressing the Andes glycoprotein (GP) (VSV-ANDV), 12 MAbs showed potent neutralization and 8 showed activity in an antibody-dependent cellular cytotoxicity reporter assay. Escape mutant analysis revealed that neutralizing MAbs targeted both the Gn and the Gc. Four MAbs that bound different epitopes were selected for preclinical studies and were found to be 100% protective against lethality in a Syrian hamster model of ANDV infection. These data suggest the existence of a wide array of neutralizing antibody epitopes on hantavirus GnGc with unique properties and mechanisms of action.

IMPORTANCE Infections with New World hantaviruses are associated with high case fatality rates, and no specific vaccine or treatment options exist. Furthermore, the biology of the hantaviral GnGc complex, its antigenicity, and its fusion machinery are poorly understood. Protective monoclonal antibodies against GnGc have the potential to be developed into therapeutics against hantaviral disease and are also great tools to elucidate the biology of the glycoprotein complex.

Subtractive immunization is useful for directing the immune response away from dominant epitopes to allow antibodies against less immunogenic antigens to be developed. It is also useful when there is a need to downplay the response to carriers added to peptides and recombinant proteins, such as Gst and Ig, to aid in production and purification. Subtractive immunization protocols also can be helpful if the target protein is part of a mixture (such as a cell lysate) or present on the surface of transfected cells.

The repetitive immunization at multiple sites (RIMMS) protocol capitalizes on the animal's innate immune system, which is genetically preprogrammed to recognize many antigens. By repetitively immunizing the animal, B cells that recognize the antigen are kept continuously expanding until the lymph nodes are harvested for hybridoma generation. This is a good method for making a more diverse repertoire of antibodies or antibodies directed against conformational epitopes.

Last week’s U.N. Special Session on the world drug problem is unlikely to overturn the existing international drug policy paradigm, argues Arturo Sarukhan, in large part because of the contradictions between U.S. domestic policy on marijuana and its international policy, and because of new drug warriors in Asia and Africa.

The wheels on the desk go round and round.

Desperate times call for desperate measures.

The 40-year-old reality TV star - who has Mason, 10, Penelope, seven, and Reign, five, with ex-boyfriend Scott Disick - has revealed she felt like she was 'on a hamster wheel' earlier in her career.

The 40-year-old reality TV star - who has Mason, 10, Penelope, seven, and Reign, five, with ex-boyfriend Scott Disick - has revealed she felt like she was 'on a hamster wheel' earlier in her career.

Two Russian hamsters prove their incredible speed going head-to-head in a race around a wheel, shoving each other aside as their competitive streak shows. BBC Earth filmed the hilarious hamster race.