Campbell, J E; McCurdy, M W; Lauzon, G; Regis, D; Wygergangs, M. Geological Survey of Canada, Open File 8714, 2020, 40 pages, https://doi.org/10.4095/327218
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/gid_327218.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/gid_327218.jpg" title="Geological Survey of Canada, Open File 8714, 2020, 40 pages, https://doi.org/10.4095/327218" height="150" border="1" /></a>

Sappin, A -A; Houlé, M G; Corrigan, D; Bédard, M -P; Wodicka, N; Brind'Amour-Côté, C; Rayner, N. Geological Survey of Canada, Scientific Presentation 115, 2020, 1 sheet, https://doi.org/10.4095/321792
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/sp0115.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/sp0115.jpg" title="Geological Survey of Canada, Scientific Presentation 115, 2020, 1 sheet, https://doi.org/10.4095/321792" height="150" border="1" /></a>

Bingham-Koslowski, N; Grey, M; Ehrman, J M; Pufahl, P. The Atlantic Geoscience Society (AGS), 47th Colloquium and Annual Meeting, program with abstracts/La Société Géoscientifique de l'Atlantique, 47th Colloquium and Annual Meeting, program with abstracts; 2021 p. 14
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/20200688.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/20200688.jpg" title="The Atlantic Geoscience Society (AGS), 47th Colloquium and Annual Meeting, program with abstracts/La Société Géoscientifique de l'Atlantique, 47th Colloquium and Annual Meeting, program with abstracts; 2021 p. 14" height="150" border="1" /></a>

Bingham-Koslowski, N; Grey, M; Pufahl, P; Ehrman, J; Strauss, A. Geoscience Canada vol. 50, 2023 p. 118, https://doi.org/10.12789/geocanj.2023.50.20
<a href="https://geoscan.nrcan.gc.ca/images/geoscan/20220635.jpg"><img src="https://geoscan.nrcan.gc.ca/images/geoscan/20220635.jpg" title="Geoscience Canada vol. 50, 2023 p. 118, https://doi.org/10.12789/geocanj.2023.50.20" height="150" border="1" /></a>

Web Services have become a standard for integration of systems in distributed environments. By using a set of open interoperability standards, they allow computer-computer interaction, regardless the programming languages and operating systems used. The Semantic Web Services, by its turn, make use of ontologies to describe their functionality in a more structural manner, allowing computers to reason about the information required and provided by them. Such a description also allows dynamic composition of several Web Services, when only one is not able to provide the desired functionality. There are scenarios, however, in which only the functional correctness is not enough to fulfill the user requirements, and a minimum level of quality should be guaranteed by their providers. In this context, this work presents an approach for dynamic Web Service composition that takes into account the composition overall quality. The proposed approach relies on a heuristics to efficiently perform the composition. In order to show the feasibility of the proposed approach, a Web Service composition application prototype was developed and experimented with public test sets, along with another approach that does not consider quality in the composition process. The results have shown that the proposed approach in general finds compositions with more quality, within a reasonable processing time.

Using pre-existing software components (COTS) to develop software systems requires the composition and adaptation of the component interfaces to solve mismatch problems. These mismatches may appear at different interoperability levels (signature, behavioural, quality of service and semantic). In this article, we define an approach which supports composition and adaptation of software components based on model transformation by taking into account the four levels. Signature and behavioural levels are addressed by means of transition systems. Context-awareness and semanticbased techniques are used to tackle quality of service and semantic, respectively, but also both consider the signature level. We have implemented and validated our proposal for the design and application of realistic and complex systems. Here, we illustrate the need to support the variability of the adaptation process in a context-aware pervasive system through a real-world case study, where software components are implemented using Windows Workflow Foundation (WF). We apply our model transformation process to extract transition systems (CA-STS specifications) from WF components. These CA-STSs are used to tackle the composition and adaptation. Then, we generate a CASTS adaptor specification, which is transformed into its corresponding WF adaptor component with the purpose of interacting with all the WF components of the system, thereby avoiding mismatch problems.

Jean Sibelius (1865-1957) was a composer who, like many of his contemporaries, drew deep inspiration from his natural environment. His passion for his home country ... Read more

The post The Influence of Nature in the Compositions of Jean Sibelius appeared first on CMUSE.

Auer Hall, Simon Music Center
Tuesday, December 3, 2024, 8 – 10pm

More info: events.iu.edu…

"O, Colored Earth" by Minnesota composer Steven Heitzeg was performed by the Minneapolis Youth Chorus during the inauguration ceremony Monday at the Fitzgerald Theater in downtown St. Paul.

Pasolini, World Music, and the Demise of the Film ComposerWhile somewhat neglected in the English-language literature on film music, Pasolini’s daring use of pre-existing music in his early films—most notably Bach’s sacred music and Vivaldi—marked a watershed in the history of the subject. It also places him alongside such figures as Godard and Kubrick (among others) in an ideal pantheon of mavericks who broke away with the traditional division of labor that underpinned the creation of film soundtracks. Pasolini’s decision to hire Morricone for the music for The Hawks and the Sparrows(1966) could have signaled a change in direction. In fact, following that first collaboration, the relationship between Pasolini and Morricone soon turned into something of an embarrassment for the composer. By the early 1970s, Morricone’s role was to merely arrange already-existing repertoires “scavenged” on various recordings by the omnivorous and ever up-to-date poet/director. Pasolini’s gleeful embrace of reproduced music calls to mind André Malraux’s “museum without walls” and his celebration of the library as both a cabinet of curiosities and workshop. Focusing on Medea(1969) and the so-called “Trilogy of Life,” this talk examines Morricone’s work as mediated and indeed guided by Pasolini the consumer, curator and ultimately producer—for Arabian Nights(1974)—of recordings.Short BioGiorgio BiancorossoGiorgio Biancorosso’s work investigates the boundaries of music and sound in the theater, cinema and digital media. He is the author of Situated Listening: The Sound of Absorption in Classical Cinema(Oxford University Press, 2016) and Remixing Wong Kar Wai: Music, Bricolage, and the Aesthetics of Oblivion(Duke University Press, 2024). Biancorosso is the co-founder and editor of the journal SSS (Sound-Stage-Screen) and the co-editor of Scoring Italian Cinema: Patterns of Collaboration(Routledge, forthcoming).Biancorosso is Professor of Music and inaugural director of the Society of Fellows at The University of Hong Kong. He is currently 2024-25 Luce East Asia Fellow in Musicology at the National Humanities Center, N.C.

dj sniff (Takuro Mizuta Lippit) Composition Colloquium (CNMAT) – Nov. 22Title of your talkParallel Traces - Records that stoped the war, trained the ear, and gave birth to turntablismshort abstractFor this occasion, I will talk about how my practice in turntablism and free improvisation led to the creation of my latest works that deal with historical narratives manifested through vinyl records and their playback devices. Namely, I will talk about Parallel Traces of the Jewel Voice (2022) and The Inverted Listening of Explosive Enemy Aircraft Sounds (2023) which both examine the critical roles that phonograph records played during WWⅡ Japan and its colonized territories, and Transformer (2023) - a sound installation that is constructed by reinterpreting stories about Grandmaster Flash’s early experiments with sound technology.short biodj sniff (Takuro Mizuta Lippit) is a musician and curator in the field of experimental electronic arts and improvised music. His work builds upon a distinct practice that combines DJing, instrument design, and free improvisation. Over the years, he has collaborated with artists such as: Evan Parker, Otomo Yoshihide, Tarek Atoui, Senyawa and many others. He holds a B.A. from Keio University Department of Aesthetics and Science of Arts, M.P.S. from NYU Interactive Telecommunications Program, and Ph.D. from De Montfort University Faculty of Computing, Engineering and Media. Alongside his artistic work, he has held positions at various institutions such as Artistic Director of STEIM – Studio for Electro-Instrumental Music Amsterdam (2007-2012), Visiting Assistant Professor at City University of Hong Kong School of Creative Media (2012-17), and Associate Professor at Kyoto Seika University (2020 - 2022). Currently based in Los Angeles, he is the Co-Director of Asian Meeting Festival (AMF) - an international music festival that brings together experimental musicians from Asia since 2005, instructor at Shared Campus Summer Schools led by Zurich University of the Arts, and a part-time lecturer at Kyoto Seika and Tokyo University of the Arts Graduate School of Global Arts.

Karola Obermueller Composition Colloquium (Morrison 250) – Nov. 15time, timbre, and transformation  abstractMy creative research revolves around a collection of themes which I return to again and again in my composing. I will discuss these areas of inquiry, how they connect to each other, and in which ways they form / govern / appear in my compositions. short bioKarola Obermüller’s composing, described by the NYT as “hyperkinetic music”, is constantly in search of the unknown. Her unique voice began forming in collages of sound made with tape recorders and evolved later with composition degrees obtained in Nuremberg, Saarbrücken, and at the Mozarteum Salzburg. Her sense of rhythm and form was forever changed by studying Carnatic and Hindustani classical music in India. Since receiving a doctorate from Harvard, Obermüller taught composition and co-directed the composition area at the University of New Mexico before joining the Department of Music at UC San Diego in 2023. Her music can be heard on CD (WERGO, New Focus Recordings, Brilliant Classics) and at karolaobermueller.net.

In the folded state, biomolecules exchange between multiple conformational states crucial for their function. However, most structural models derived from experiments and computational predictions only encode a single state. To represent biomolecules accurately, we must move towards modeling and predicting structural ensembles. Information about structural ensembles exists within experimental data from X-ray crystallography and cryo-electron microscopy. Although new tools are available to detect conformational and compositional heterogeneity within these ensembles, the legacy PDB data structure does not robustly encapsulate this complexity. We propose modifications to the macromolecular crystallographic information file (mmCIF) to improve the representation and interrelation of conformational and compositional heterogeneity. These modifications will enable the capture of macromolecular ensembles in a human and machine-interpretable way, potentially catalyzing breakthroughs for ensemble–function predictions, analogous to the achievements of AlphaFold with single-structure prediction.

SnGe4N4O4 was synthesized at high pressure (16 and 20 GPa) and high temperature (1200 and 1500°C) in a large-volume press. Powder X-ray diffraction experiments using synchrotron radiation indicate that the derived samples are mixtures of known and unknown phases. However, the powder X-ray diffraction patterns are not sufficient for structural characterization. Transmission electron microscopy studies reveal crystals of several hundreds of nanometres in size with different chemical composition. Among them, crystals of a previously unknown phase with stoichiometry SnGe4N4O4 were detected and investigated using automated diffraction tomography (ADT), a three-dimensional electron diffraction method. Via ADT, the crystal structure could be determined from single nanocrystals in space group P63mc, exhibiting a nolanite-type structure. This was confirmed by density functional theory calculations and atomic resolution scanning transmission electron microscopy images. In one of the syntheses runs a rhombohedral 6R polytype of SnGe4N4O4 could be found together with the nolanite-type SnGe4N4O4. The structure of this polymorph was solved as well using ADT.

The synthetic availability of mol­ecular water oxidation catalysts containing high-valent ions of 3d metals in the active site is a prerequisite to enabling photo- and electrochemical water splitting on a large scale. Herein, the synthesis and crystal structure of di­ammonium {μ-1,3,4,7,8,10,12,13,16,17,19,22-dodeca­aza­tetra­cyclo­[8.8.4.13,17.18,12]tetra­cosane-5,6,14,15,20,21-hexa­onato}ferrate(IV) acetic acid tris­olvate, (NH4)2[FeIV(C12H12N12O6)]·3CH3COOH or (NH4)2[FeIV(L–6H)]·3CH3COOH is reported. The FeIV ion is encapsulated by the macropolycyclic ligand, which can be described as a dodeca-aza-quadricyclic cage with two capping tri­aza­cyclo­hexane fragments making three five- and six six-membered alternating chelate rings with the central FeIV ion. The local coord­ination environment of FeIV is formed by six deprotonated hydrazide nitro­gen atoms, which stabilize the unusual oxidation state. The FeIV ion lies on a twofold rotation axis (multiplicity 4, Wyckoff letter e) of the space group C2/c. Its coordination geometry is inter­mediate between a trigonal prism (distortion angle φ = 0°) and an anti­prism (φ = 60°) with φ = 31.1°. The Fe—N bond lengths lie in the range 1.9376 (13)–1.9617 (13) Å, as expected for tetra­valent iron. Structure analysis revealed that three acetic acid mol­ecules additionally co-crystallize per one iron(IV) complex, and one of them is positionally disordered over four positions. In the crystal structure, the ammonium cations, complex dianions and acetic acid mol­ecules are inter­connected by an intricate system of hydrogen bonds, mainly via the oxamide oxygen atoms acting as acceptors.

In the structure of the title salt, {[Ba(μ3-C3H2N3O2)2(μ-H2O)(H2O)]·H2O}n, the barium ion and all three oxygen atoms of the water mol­ecules reside on a mirror plane. The hydrogen atoms of the bridging water and the solvate water mol­ecules are arranged across a mirror plane whereas all atoms of the monodentate aqua ligand are situated on this mirror plane. The distorted ninefold coord­ination of the Ba ions is completed with four nitroso-, two carbonyl- and three aqua-O atoms at the distances of 2.763 (3)–2.961 (4) Å and it is best described as tricapped trigonal prism. The three-dimensional framework structure is formed by face-sharing of the trigonal prisms, via μ-nitroso- and μ-aqua-O atoms, and also by the bridging coordination of the anions via carbonyl-O atoms occupying two out of the three cap positions. The solvate water mol­ecules populate the crystal channels and facilitate a set of four directional hydrogen bonds. The principal Ba–carbamoyl­cyano­nitro­somethanido linkage reveals a rare example of the inherently polar binodal six- and three-coordinated bipartite topology (three-letter notation sit). It suggests that small resonance-stabilized cyano­nitroso anions can be utilized as bridging ligands for the supra­molecular synthesis of MOF solids. Such an outcome may be anti­cipated for a broader range of hard Lewis acidic alkaline earth metal ions, which perfectly match the coordination preferences of highly nucleophilic nitroso-O atoms. Thermal analysis reveals two-stage dehydration of the title compound (383 and 473 K) followed by decomposition with release of CO2, HCN and H2O at 558 K.

The influence of various combinations of residual stress, composition and grain interaction gradients in polycrystalline materials with cubic symmetry on energy-dispersive X-ray stress analysis is theoretically investigated. For the evaluation of the simulated sin2ψ distributions, two different strategies are compared with regard to their suitability for separating the individual gradients. It is shown that the separation of depth gradients of the strain-free lattice parameter a0(z) from residual stress gradients σ(z) is only possible if the data analysis is carried out in section planes parallel to the surface. The impact of a surface layer z* that is characterized by a direction-dependent grain interaction model in contrast to the volume of the material is quantified by comparing a ferritic and an austenitic steel, which feature different elastic anisotropy. It is shown to be of minor influence on the resulting residual stress depth profiles if the data evaluation is restricted to reflections hkl with orientation factors Γhkl close to the model-independent orientation Γ*. Finally, a method is proposed that allows the thickness of the anisotropic surface layer z* to be estimated on the basis of an optimization procedure.

Predicting crystal symmetry simply from chemical composition has remained challenging. Several machine-learning approaches can be employed, but the predictive value of popular crystallographic databases is relatively modest due to the paucity of data and uneven distribution across the 230 space groups. In this work, virtually all crystallographic information available to science has been compiled and used to train and test multiple machine-learning models. Composition-driven random-forest classification relying on a large set of descriptors showed the best performance. The predictive models for crystal system, Bravais lattice, point group and space group of inorganic compounds are made publicly available as easy-to-use software downloadable from https://gitlab.com/vishsoft/cosy.

The ingredient reportedly delivers the a premium taste profile closest to sugar, even at high sugar replacement levels, while offering a more cost-effective solution than other premium sweeteners.

Say hello to Composition, our new multipurpose WordPress theme that has all the right ingredients for building any kind of website. What is a Multipurpose WordPress Theme? Simply put, a multipurpose WordPress theme is designed with maximum flexibility in mind. This means that you can use Composition for a wide range of websites including ones for Businesses, Portfolios, […]

Comprehensive Proteomics Analysis of the Hemolymph Composition of Sugar-Fed Aedes aegypti Female and Male Mosquitoes  ACS Publications

Compositions provide a soundtrack for the Cassini probe’s final plunge into planetToronto, ON –After centuries of looking with awe and wonder at the beauty of Saturn and its rings, we can now listen to them, thanks to the efforts of astrophysicists at the University of Toronto (U of T). “To celebrate the Grand Finale of […]

Conformance of ITU-T H.810 personal health system: Personal Health Devices interface Part 5L: Body composition analyser

The fungus has decimated chestnut trees in the national park, and larger trees have been slow to replace them.

The purpose of this study was to assess the experiences of 1st-year college composition writing students in a synchronous online remote learning environment. The researcher, through this qualitative study, used a case study design to examine the quality of the learning experiences of the participant....

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Alexi Kenney, a distinguished violinist, artist, and curator, will speak about his work.

Zosha Di Castri, a Canadian “composer of riotously inventive works” (The New Yorker), currently lives in New York. Her music has been performed across Canada, the United States, South America, Asia, and Europe and extends beyond purely concert music, including projects with electronics, sound arts, and collaborations with video and dance that encourage audiences to feel “compelled to return for repeated doses” (The Arts Desk). She is currently the Francis Goelet Associate Professor of Music at Columbia University and a 2023 American Academy of Arts and Letters Goddard Lieberson fellow. Zosha’s current projects include a large chamber work commissioned by the LA Phil and conducted by John Adams, receiving its premiere in spring 2024; a Koussevitzky commission from the Library of Congress for percussionist Steve Schick and ensemble, and upcoming collaborations with the Bozzini Quartet and Ensemble Paramirabo/Totem. Zosha recently curated an event showcasing her work as part of the New York Philharmonic’s 2023 Nightcap series. Her 2022 work, In the Half-Light, a song cycle for soprano Barbara Hannigan, with libretto by Tash Aw, was premiered by the Toronto Symphony Orchestra and will be performed again this season by the Montreal Symphony Orchestra. Other recent projects include We live the opposite daring for six voices written for Ekmeles, time>>T. - - I. - - M.(time) - - E, a commission for largechamber ensemble premiered by the Grossman Ensemble in Chicago; Hypha, a quartet for clarinet, violin, cello, and piano/keyboard commissioned by the Chamber Music Society of Lincoln Center; and Pentimento, a short piece for orchestra commissioned by the WDR Sinfonieorchester for its 75th anniversary. In July 2019, Zosha’s Long Is the Journey, Short Is the Memory for orchestra and chorus opened the first night of the BBC Proms at Royal Albert Hall, conducted by Karina Canellakis with the BBC Symphony and BBC Singers. Other large-scale projects include a 25-minute piece for soprano, recorded narrator and orchestra entitled Dear Life, based on a short-story by Alice Munro, and an evening-length new music theater piece, Phonobellow, co-written with David Adamcyk for the International Contemporary Ensemble with performances in New York and Montreal. Phonobellow features five musicians, a large kinetic sound sculpture, electronics, and video in a reflection on the influence of photography and phonography on human perception. Zosha’s orchestral compositions have been commissioned by John Adams, the Toronto Symphony, the San Francisco Symphony, New World Symphony, Esprit Orchestra, the National Arts Centre Orchestra, the Orchestre symphonique de Montréal, and the BBC, and have been featured by the the New York Philharmonic, Tokyo Symphony, Amazonas Philharmonic, The Philadelphia Orchestra, and the Cabrillo Festival Orchestra, among others. She has made appearances with the Chicago Symphony, the LA Philharmonic, and the San Francisco Contemporary Music Players in their chamber music series, and has worked with many leading new music groups, including Talea Ensemble, Wet Ink Ensemble, Ekmeles, Yarn/Wire, the NEM, Ensemble Cairn, and JACK and Parker Quartets. Other recent projects include a commission titled Hunger for the Orchestre symphonique de Montréal with improvised drummer, designed to accompany Peter Foldes’ 1973 eponymous silent film; a string quartet for the Banff International String Quartet Competition; a piece for Yarn/Wire for two pianists, two percussionists, and electronics premiered at Zosha’s Miller Theatre Composer Portrait concert; a solo piano work for Julia Den Boer commissioned by the Yvar Mikhashoff Trust Fund, and a string octet premiered by JACK Quartet and Parker Quartet at the Banff Centre. She was the recipient of the Jules Léger Prize for New Chamber Music for her work Cortège in 2012, and participated in Ircam’s Manifeste Festival in Paris, writing an interactive electronic work for Thomas Hauert’s dance company, ZOO. Zosha’s debut album Tachitipo was released on New Focus Recordings in November 2019 to critical acclaim, and the title track was nominated for The JUNO Awards’ 2021 Classical Composition of the Year. Tachitipo was named in Best of 2019 lists by The New Yorker, I Care if You Listen, AnEarful, Sequenza21, and New York Music Daily, and praised as “a formidable statement. It is so comprehensively realized, institutionally ratified, and sensitive to the creative exigencies of the 21st century that one wants to send a copy of it to the publishers of textbooks for music history survey courses in the hope that it will be included in a last chapter or two.” (I Care if You Listen) Zosha is a recipient of the 2021 Guggenheim Fellowship and was an inaugural fellow at the Institute for Ideas and Imagination in Paris in 2018-19. She completed her Bachelors of Music in Piano Performance and Composition at McGill University, and her DMA in Composition at Columbia University. Born in St. Albert, Alberta, Canada, Zosha currently lives with her family in New York City.

Lars Jønson
May 1, 2007; 6:798-811
Research

Voltage-gated Cav1 and Cav2 Ca2+ channels are comprised of a pore-forming α1 subunit (Cav1.1-1.4, Cav2.1-2.3) and auxiliary β (β1-4) and α2δ (α2δ−1−4) subunits. The properties of these channels vary with distinct combinations of Cav subunits and alternative splicing of the encoding transcripts. Therefore, the impact of disease-causing mutations affecting these channels may depend on the identities of Cav subunits and splice variants. Here, we analyzed the effects of a congenital stationary night blindness type 2 (CSNB2)-causing mutation, I745T (IT), in Cav1.4 channels typical of those in human retina: Cav1.4 splice variants with or without exon 47 (Cav1.4+ex47 and Cav1.4Δex47, respectively), and the auxiliary subunits, β2X13 and α2δ-4. We find that IT caused both Cav1.4 splice variants to activate at significantly more negative voltages and with slower deactivation kinetics than the corresponding WT channels. These effects of the IT mutation, along with unexpected alterations in ion selectivity, were generally larger in channels lacking exon 47. The weaker ion selectivity caused by IT led to hyperpolarizing shifts in the reversal potential and large outward currents that were evident in channels containing the auxiliary subunits β2X13 and α2δ-4 but not in those with β2A and α2δ-1. We conclude that the IT mutation stabilizes channel opening and alters ion selectivity of Cav1.4 in a manner that is strengthened by exclusion of exon 47 and inclusion of β2X13 and α2δ-4. Our results reveal complex actions of IT in modifying the properties of Cav1.4 channels, which may influence the pathological consequences of this mutation in retinal photoreceptors.

The plasma membrane of a cell is characterized by an asymmetric distribution of lipid species across the exofacial and cytofacial aspects of the bilayer. Regulation of membrane asymmetry is a fundamental characteristic of membrane biology and is crucial for signal transduction, vesicle transport, and cell division. The type IV family of P-ATPases, or P4-ATPases, establishes membrane asymmetry by selection and transfer of a subset of membrane lipids from the lumenal or exofacial leaflet to the cytofacial aspect of the bilayer. It is unclear how P4-ATPases sort through the spectrum of membrane lipids to identify their desired substrate(s) and how the membrane environment modulates this activity. Therefore, we tested how the yeast plasma membrane P4-ATPase, Dnf2, responds to changes in membrane composition induced by perturbation of endogenous lipid biosynthetic pathways or exogenous application of lipid. The primary substrates of Dnf2 are glucosylceramide (GlcCer) and phosphatidylcholine (PC, or their lyso-lipid derivatives), and we find that these substrates compete with each other for transport. Acutely inhibiting sphingolipid synthesis using myriocin attenuates transport of exogenously applied GlcCer without perturbing PC transport. Deletion of genes controlling later steps of glycosphingolipid production also perturb GlcCer transport to a greater extent than PC transport. In contrast, perturbation of ergosterol biosynthesis reduces PC and GlcCer transport equivalently. Surprisingly, application of lipids that are poor transport substrates differentially affects PC and GlcCer transport by Dnf2, thus altering substrate preference. Our data indicate that Dnf2 exhibits exquisite sensitivity to the membrane composition, thus providing feedback onto the function of the P4-ATPases.

Mass spectrometry has become an indispensable tool for the characterization of glycosylation across biological systems. Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind. Although glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not. This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria. As such, computational approaches to examine bacterial glycosylation and identify chemically diverse glycans are desperately needed. Here we describe the use of wide-tolerance (up to 2000 Da) open searching as a means to rapidly examine bacterial glycoproteomes. We benchmarked this approach using N-linked glycopeptides of Campylobacter fetus subsp. fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching. Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans). Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences. Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.

A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine. At present, these files are mainly built from searching the literature and/or querying data sources focused on posttranslational modifications. Most glycoproteomics search engines include a default composition file that is readily used when processing MS data. We introduce here a glycan composition visualizing and comparative tool associated with the GlyConnect database and called GlyConnect Compozitor. It offers a web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions. The tool takes advantage of compositions being related to one another through shared monosaccharide counts and outputs interactive graphs summarizing information searched in the database. These results provide a guide for selecting or deselecting compositions in a file in order to reflect the context of a study as closely as possible. They also confirm the consistency of a set of compositions based on the content of the GlyConnect database. As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application. It is also directly accessible from the GlyConnect database.

More than 250 years after a teenage Mozart wrote "Serenade in C," a copy of the piece has surfaced in the collections of a German library

Composition (per 100 gm) Of 9 Common Vegetables.

Understanding food and...

On Monday, the government extended the due date for filing of annual GST returns for 2019-20 by composition dealers to 31 October. It is the second extension in as many months given by the government. Earlier, the original deadline of 15