Sorghum, a short-day tropical plant, has been adapted for temperate grain production, in particular through the selection of variants at the MATURITY loci (Ma1–Ma6) that reduce photoperiod sensitivity. Ma3 encodes phytochrome B (phyB), a red/far-red photochromic biliprotein photoreceptor. The multi-domain gene product, comprising 1178 amino acids, autocatalytically binds the phytochromobilin chromophore to form the photoactive holophytochrome (Sb.phyB). This study describes the development of an efficient heterologous overproduction system which allows the production of large quantities of various holoprotein constructs, along with purification and crystallization procedures. Crystals of the Pr (red-light-absorbing) forms of NPGP, PGP and PG (residues 1–655, 114–655 and 114–458, respectively), each C-terminally tagged with His6, were successfully produced. While NPGP crystals did not diffract, those of PGP and PG diffracted to 6 and 2.1 Å resolution, respectively. Moving the tag to the N-terminus and replacing phytochromobilin with phycocyanobilin as the ligand produced PG crystals that diffracted to 1.8 Å resolution. These results demonstrate that the diffraction quality of challenging protein crystals can be improved by removing flexible regions, shifting fusion tags and altering small-molecule ligands.

Days before Kristine (international name: Trami) developed into a severe tropical storm, the Philippine Sea was warmer than usual. Under the warming climate, the typhoon-prone Bicol Region is more vulnerable with decimated forests. Both Camarines Sur and Albay saw a net loss of tree cover from 2000 to 2020, according to Global Forest Watch.

Aputure’s been coming pretty thick and fast on the announcements lately, and now they’ve announced their new Light Storm 60D daylight and 60X bi-colour adjustable focusing LED lights. As the name suggests, these are 60 Watt LEDs, and everything is built inside the head, meaning there’s no external control unit to have to deal with. […]

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