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On most dimensions, the policy mix of incoming president is positive for US equities

Star-studded nuptials started in March with a three-day pre-wedding party with a performance by superstar Rihanna

Voice of Indigenous Peoples

Premiere Episode Titled: "A Midwife's Mission in Cameroon"

GRE Alpha North America and Global Lighting and Controls (GL&C) have announced their partnership.

Fulfilling short-term needs while maintaining long-term priorities has been an ongoing challenge for organizations since the onset of the pandemic. The global response to COVID-19 has slowed the day-to-day operations of many companies but amplified the progression of transformations that were already brewing before the outbreak.

Ircam Amplify's AI Speech Detector identifies a wider range of models used to generate fake voices.

This is my Wishlist of tech I'd like to see at HP Amplify later this week. #hp #hpamplify #ai #ar #vr

When Ebrahim Rasool returns to Washington in December as South Africa's ambassador, one of his primary goals is to reposition Pretoria as a "moral superpower"

People who experience depression and concussions simultaneously have significantly worse symptoms for both conditions and disruptions to brain connectivity, according to a study by researchers at Penn State.

Ajungi – pronounced AH-YUNG-EE – is a Nunavut-based music collective made up of up-and-coming artists from across the territory. The concept was created by artist and entrepreneur Thor Simonsen, the creative director of Hitmakerz, an Iqaluit-based record label that delivers music workshops to remote communities across Nunavut and records, produces, and releases music by many Inuit artists.

Simonsen joins us to talk about the project's inception and goals, what the workshops and recording sessions entail, how they benefit the artists' careers and communities, and a lot more.

http://canadianmusician.com http://ajungi.ca

Groundwater pumping and irrigation can disrupt natural atmospheric processes, affect the whole water cycle, and potentially worsen water shortages during heatwaves, a new study suggests. The findings contribute to our understanding of how to manage water resources under future climate change conditions. The study shows how some of the most intensively water managed areas of Europe — such as the Iberian Peninsula — could be affected by extraction of groundwater during years when conditions are especially hot and dry, potentially amplifying water scarcity in already-stressed regions.

Magnesite has long been known as a CO2 absorber, but the slow rate of its growth made it a poor carbon storage option. Until now.

CIWEM Environmental Photographer of the Year contest captures how humanity is coping with climate change.

19th Annual LES Silicon Valley Chapter Conference "5G and IP - Are You Ready?" – 17 April 2019

An electronic circuit for amplifying signals with two components in phase quadrature, which includes: a feedback amplifier with a feedback capacitor; a switch that drives charging and discharging of the feedback capacitor; an additional capacitor; and a coupling circuit, which alternatively connects the additional capacitor in parallel to the feedback capacitor or else decouples the additional capacitor from the feedback capacitor. The switch opens at a first instant, where a first one of the two components assumes a first zero value; the coupling circuit decouples the additional capacitor from the feedback capacitor in a way synchronous with a second instant, where the first component assumes a second zero value.

Embodiments of the disclosure encompass methods of amplifying nucleic acid from one or more cells using MALBAC (multiple annealing and looping-based amplification cycles) primers. In particular embodiments, the nucleic acid is amplified as amplicons in a linear manner. Specific embodiments include the removal or effective destruction of nonlinearly produced amplicons.

Dans ce monde où les business model des entreprises et encore plus les technologies sont en rupture, le challenge est le même : rester en contact avec le consommateur hyper connecté d’aujourd’hui. Ce challenge peut être vu comme un véritable cauchemar ou à l’inverse, considéré comme la prochaine grande opportunité pour les entreprises. Elles sont obligées d’aller au-delà des prévisions, en identifiant et capitalisant sur des opportunités que d’autres entreprises ne voient pas.

How best for the party to get its message across to voters.

Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective processing of single cells. In particular, the concept of using boosting/carrier channels in isobaric labeling to increase the sensitivity in MS detection has also been increasingly used for quantitative proteomic analysis of small-sized samples including single cells. However, the full potential of such boosting/carrier approaches has not been significantly explored, nor has the resulting quantitation quality been carefully evaluated. Herein, we have further evaluated and optimized our recent boosting to amplify signal with isobaric labeling (BASIL) approach, originally developed for quantifying phosphorylation in small number of cells, for highly effective analysis of proteins in single cells. This improved BASIL (iBASIL) approach enables reliable quantitative single-cell proteomics analysis with greater proteome coverage by carefully controlling the boosting-to-sample ratio (e.g. in general <100x) and optimizing MS automatic gain control (AGC) and ion injection time settings in MS/MS analysis (e.g. 5E5 and 300 ms, respectively, which is significantly higher than that used in typical bulk analysis). By coupling with a nanodroplet-based single cell preparation (nanoPOTS) platform, iBASIL enabled identification of ~2500 proteins and precise quantification of ~1500 proteins in the analysis of 104 FACS-isolated single cells, with the resulting protein profiles robustly clustering the cells from three different acute myeloid leukemia cell lines. This study highlights the importance of carefully evaluating and optimizing the boosting ratios and MS data acquisition conditions for achieving robust, comprehensive proteomic analysis of single cells.

Chia-Feng Tsai
May 1, 2020; 19:828-838
Research

Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective processing of single cells. In particular, the concept of using boosting/carrier channels in isobaric labeling to increase the sensitivity in MS detection has also been increasingly used for quantitative proteomic analysis of small-sized samples including single cells. However, the full potential of such boosting/carrier approaches has not been significantly explored, nor has the resulting quantitation quality been carefully evaluated. Herein, we have further evaluated and optimized our recent boosting to amplify signal with isobaric labeling (BASIL) approach, originally developed for quantifying phosphorylation in small number of cells, for highly effective analysis of proteins in single cells. This improved BASIL (iBASIL) approach enables reliable quantitative single-cell proteomics analysis with greater proteome coverage by carefully controlling the boosting-to-sample ratio (e.g. in general <100x) and optimizing MS automatic gain control (AGC) and ion injection time settings in MS/MS analysis (e.g. 5E5 and 300 ms, respectively, which is significantly higher than that used in typical bulk analysis). By coupling with a nanodroplet-based single cell preparation (nanoPOTS) platform, iBASIL enabled identification of ~2500 proteins and precise quantification of ~1500 proteins in the analysis of 104 FACS-isolated single cells, with the resulting protein profiles robustly clustering the cells from three different acute myeloid leukemia cell lines. This study highlights the importance of carefully evaluating and optimizing the boosting ratios and MS data acquisition conditions for achieving robust, comprehensive proteomic analysis of single cells.

JC Henquin
Nov 1, 2000; 49:1751-1760
Articles

Amplify is an enterprise software solution for Strategy Execution Management with features such as business goals and target setting, dependency maps, schedule, benefit and cost tracking, stage gate workflows with approvals, and a suite of reporting options.

Since Samsung’s acquisition of HARMAN less than two years ago, the two companies have worked closely to create and implement connective technologies that align with what consumers want today – and in the future. As technology grows ever more capable,...

Cloud technology, big data and artificial intelligence are empowering automakers to create in-car experiences that rival the at-home experience for end users. Automakers themselves can leverage these technologies over time to consistently and ...

Eric Alterman

The post The Press Is Amplifying a Dangerous Know-Nothing Ideology appeared first on The Nation.

Trading restrictions did not work in the past; there is less reason for them to work this time

Glenn Brown of Twitter Amplify explains how the social media service is giving brands and users a digital megaphone to tweet during broadcasts and major events.

Online Resource

Panel:

• AJ O’Neal
• Aimee Knight
• Joe Eames

Special Guests: Nader Dabit

In this episode, the panel talks with programmer, Nader Dabit, who has been with Amazon’s AWS for the past six months. They discuss the new innovations that Amazon is currently working on, and the exciting new projects that Nader gets to be involved with. Check out this episode to hear all the latest!

Show Topics:

1:45 – There are two main things that Nader works with. Check out this timestamp to see what they are.

3:29 – AJ to Nader: Tell me more about manage cloud. I am not sure about Cognito.

3:56 – Yes, Cognito is used by/through Amazon.

5:06 – What are the other manage cloud services that companies want to offer through the tools you have?

5:12 – Nader answers AJ’s question.

7:30 – Can you give me more specifics on the storage solutions you are offering?

8:03 – Nader answers AJ’s question. People store websites there for example. Frontend developers are using S3 buckets, and they are using the library, which is a storage solution.

9:10 – AJ and Nader are having a dialogue between different situations, and Nader is giving the solutions to those hypothetical situations.

10:17 – AJ: “I am interested in what you are talking about AppSync. Can you tell me how that works?” AJ is picking Nader’s brain about how AppSync works.

11:05 – Nader: “It is a single API layer for a point of entry. You can have multi-data sources.” Nader continues, in detail, answering AJ’s question.

12:36 – AJ: As a frontend developer, it sounds like I will have to become familiar with the backend, too. How is it providing the most value? What is it that I do not have to touch, because I am using this?

15:37 – How would these relations work? As a frontend developer, and I do not want to learn sequel, how would that might look like; currently or in the future? How do you extract that knowledge?

16:18 – Yes, it is not an easy solution to solve. Nader goes into detail about how he would approach this situation.

18:26 – AJ: Are these resolvers written in JavaScript?

22:04 – Acronym fun!

22:45 – Node

23:51 – Summarizing these pasts 20-some-minutes: Off-Storage, AppSync, Landis, and others are what people are using Amplify for. New Question/New Topic: Simplify.

25:45 – AWS MOBILE – is not mobile specific.

26:44 – If you are using Angular, we have a plugin in Angular to help you. We also have that for React and Vue as well.

27:52 – Advertisement

28:56 – What should we be talking about?

29:04 – Let’s talk about Amazon’s Lex, Chat Bot. Nader goes into full detail of this service.

33:52 – Apple T.V.

34:00 – AJ: Sounds like this is more platform/ more agnostic than getting different things to come together, and the Microsoft one is more hybrid and the Amazon one is more open?

35:13 – Joe, let’s go back to what you had to ask.

35:28 – Nader, you talked about PUSH notifications earlier. What is Pub/Sub?

36:30 – Is this like traditional hooks? Or custom?

37:25 – What is the “stuff” that gets you up in the morning and gets you excited to go to work at AWS?

38:40 – Nader: I really had no desire to change career paths, but it happened.

41:30 – AJ: I totally agree with the idea in that finding the common patterns, so that way someone on the lower-level can participate. AJ wants a platform that is open or purchase that can offer some of these benefits. It could be open-source or you used to buy the different tools.

43:27 AJ: What about for the hobbyist?

43:40 – Nader: I agree, that would be really nice. I can’t think of any free services that would be nice.

44:03 AJ – Not free in “free,” but “free” towards the idea of “free speech.” They would all be available and you get to choose what works well for you.

45:00 – SHOUTOUT to LISTENERS: Have an idea about this? Shoot the panel an e-mail!

45:33 – Hopefully this opens the listeners’ eyes to what’s out there.

45:48 – Cloud services.

46:55 – Innovation follows niche markets. When something gets big and established, innovation comes to a plateau. The innovation will develop in a new economic area like hydraulics. AJ thinks a niche will develop.

49:03 – Is there anything, Dabit, which you would like to talk about?

49:15 – Can we talk about AI as a service?

51:10 – Nader saw a demonstration recently.

52:26 – Hearing these implications is so cool, but when it comes to ML a panelist dabbled a little bit. He watched some videos, unless you want to devote a year or two to learning it then it’s too complex to put together. Do you have to be genius-level to get through?

53:29 – ML you are passing data. Nader is not quite sure.

56:00 Nader just did a blog post  check-it-out!

56:49 – Let’s do Picks!

56:50 – Advertisement

Links:

• Nader Dabit’s Twitter
• Nader Dabit’s Medium
• Nader Dabit’s LinkedIn
• Nader Dabit’s GitHub
• Nader Dabit’s Website
• Nader Dabit’s YouTube channel
• Nader Dabit’s Egg Head
• JavaScript
• Amazon’s Cognito
• AWS AppSyncNode
• Landis
• AWS Mobile
• Vue
• Angular
• Amazon’s Lex – Chat Bot
• Apple T.V.
• Push Notifications
• Pub/Sub
• AWS’ Artificial Intelligence (AI)

Sponsors:

• Kendo UI
• Sentry
• Digital Ocean

Picks:

AJ O’Neal

• Blog / Thoughty 2’s Video: Pop Music
• The Innovator’s Solution / Book
• The Innovator’s Dilemma / Book

Joe Eames

• Framework Summit - Tickets are still available!
• Movie: Equalizer 2

Nader Dabit

• Finland – Graph Talks Conference, October
• AWS – San Francisco - LOFT