en Sport | Marco Jansen hopes for better showing against spin as T20 series takes Highveld turn By www.news24.com Published On :: Tuesday Nov 12 2024 15:29:43 Proteas all-rounder Marco Jansen, while acknowledging that spin has been a challenge for them in the ongoing T20 series against India, reckons things could be a bit different for Wednesday's third T20 in Centurion. Full Article
en Sport | Prioritise players' progress over your own pockets, Broos urges agents as starlets shine in the PSL By www.news24.com Published On :: Tuesday Nov 12 2024 17:07:55 Bafana Bafana coach Hugo Broos has welcomed the number of young players who are given a chance and backed in the premier division, but has warned that to build on their progress, agents must prioritise players' development over their personal enrichment. Full Article
en Sport | Difference between good and great is handling pressure, says Kaizer Chiefs legend Baloyi By www.news24.com Published On :: Tuesday Nov 12 2024 19:39:57 Former Kaizer Chiefs legend Brian Baloyi says that holding the Kaizer Chiefs No 1 jersey requires a hardened mentality, but he is upbeat over Amakhosi's chances this season. Full Article
en Sport | The battle cries begin: Springboks 'not unbeatable' says England winger Freeman By www.news24.com Published On :: Tuesday Nov 12 2024 22:03:56 The first battle cry has sounded ahead of the showdown at Twickenham on Saturday, with English winger Tommy Freeman reckoning that the Springboks are 'still human' and 'still playing the same game'. Full Article
en Sport | Makgopa's rise evidence of Broos' Bafana impact: 'You don't want to disappoint such a person' By www.news24.com Published On :: Wednesday Nov 13 2024 05:00:31 Evidence Makgopa wore a sheepish smile when Bafana Bafana coach Hugo Broos showered him with praise before literally patting the forward on the back, making him blush at Dobsonville Stadium. Full Article
en Sport | BIG PREVIEW | SA v India: Jansen wary of India's blazing batters as Centurion run fest awaits By www.news24.com Published On :: Wednesday Nov 13 2024 05:01:33 Wednesday's third T20 between South Africa and India at SuperSport Park in Centurion is shaping up to be a run-drenched one, especially if the weather holds and SA's bowling centre doesn't. Full Article
en Sport | Springbok injuries: Sacha on the mend, doubt remains over Kitshoff future By www.news24.com Published On :: Wednesday Nov 13 2024 09:30:19 Springbok team doctor Jerome Mampane says that injured utility back Sacha Feinberg-Mngomezulu is recovering well. Full Article
en French mention of Farahat’s Book: Iran : « Femme, vie, liberté ! » By cynthiafarahat.com Published On :: Mon, 17 Oct 2022 21:32:11 +0000 By JEAN-PIERRE LLEDO MABATIM Oct. 16, 2022 Toutes les informations qui nous parviennent d’Iran nous disent que cette nouvelle révolte est totalement inédite. Elle se distingue de toutes les précédentes parce que ce sont les femmes qui en ont pris l’initiative et plus encore parce que ce qui est ciblé n’est pas seulement le pouvoir, […] Full Article Featured Articles
en Egyptian Government Television Episode About Farahat’s Book By cynthiafarahat.com Published On :: Mon, 31 Oct 2022 13:25:24 +0000 Cairo, Egypt Oct. 28, 2022 On Egyptian TV show, “Headlines,” Dr. Hossam Farouk dedicated an episode to discussing national and international coverage of the Secret Apparatus: The Muslim Brotherhood’s Industry of Death. It was a great honor having the book featured on this great show. Full Article Featured Articles
en The West, a New Haven for the ‘Muslim Brotherhood’s Planetary Nebula’? By cynthiafarahat.com Published On :: Thu, 01 Dec 2022 13:59:01 +0000 By SADF – Nov. 30, 2022 Online conversation on The West, a New Haven for the ‘Muslim Brotherhood’s Planetary Nebula’? On November the 29th at 16H.00 (CET), SADF promoted an online conversation in the web platform StreamYard titled ‘The West, a New Haven for the ‘Muslim Brotherhood’s Planetary Nebula’?’ The conversation included the Member of the European Parliament (EPP, […] Full Article Featured Articles
en The Secret Apparatus Book Review: Conspiracy? Yes, A Century of Muslim Brotherhood Jihad By cynthiafarahat.com Published On :: Sat, 10 Dec 2022 15:53:06 +0000 By Walter Donway, Savvy Street originally published on Dec. 4, 2022 Book Review: The Secret Apparatus: The Muslim Brotherhood’s Industry of Death by Cynthia Farahat, Post Hill Press, Bombardier Books (2022) A grim irony of twentieth-century history (but, of course, also predating it) is that the most horrendous threats to humanity have simply been too monstrously evil […] Full Article 9/11 Academia Afghanistan Al-Azhar University Al-Qaeda CAIR Infiltration Muslim Brotherhood
en Newsroom: TikTok surpassed YouTube as the third-most-popular influencer platform among US marketers in 2021 By www.emarketer.com Published On :: Mon, 24 Jan 2022 04:01:00 GMT TikTok will have more US users than Snapchat and Pinterest in 2022   January 24, 2022 (New York, NY) – YouTube is the “OG†of influencer marketing platforms, and it’s […] Full Article
en Newsroom: Insider Intelligence Slashes Ad Spending Forecast for Russia and Eastern Europe Amid Conflict By www.emarketer.com Published On :: Thu, 31 Mar 2022 04:01:00 GMT Total media ad spend in Russia to drop nearly 50%   March 30, 2022 (New York, NY) – Insider Intelligence expects the ongoing war in Ukraine to have a significant […] Full Article
en Newsroom: TikTok Ad Revenues to Surpass Twitter and Snapchat Combined By www.emarketer.com Published On :: Mon, 11 Apr 2022 04:01:00 GMT Worldwide revenues will triple in 2022   April 11, 2022 (New York, NY) – TikTok’s worldwide ad revenues will triple this year and surpass that of two competitors combined, according […] Full Article
en Newsroom: 34 Million US Adults Own Cryptocurrency By www.emarketer.com Published On :: Wed, 20 Apr 2022 04:01:00 GMT 3.6 million will pay with crypto in US; payment value to spike 70% in 2022   April 20, 2022 (New York, NY) – Cryptocurrency usage will continue its meteoric rise, […] Full Article
en Commentary: Secret Service Agents Placed on Leave After Trump Assassination Attempt By deneenborelli.com Published On :: Fri, 23 Aug 2024 19:57:50 +0000 Commentary by Susan Crabtree originally published by RealClearPolitics and RealClearWire Three weeks ago, Acting Secret Service Director Ronald Rowe angrily pushed back on senators’ calls to immediately fire or discipline key agents directly responsible for the security failures that led to the assassination attempt against former President Trump at last month’s campaign rally in Butler, … Full Article Commentaries News
en Commentary: The Tale of Two Conventions By deneenborelli.com Published On :: Fri, 23 Aug 2024 20:09:01 +0000 Commentary by Newt Gingrich originally published by RealClearPolicy and RealClearWire Historians will look at the 2024 Democratic and Republican national conventions as harbingers of profound changes in American politics and government. Callista and I participated in the Republican National Convention in Milwaukee, so we got a good sense of who was there and what was … Full Article Commentaries News
en Commentary: Harris Pushed To Explain Policy Shifts in First Interview as Dem Nominee By deneenborelli.com Published On :: Fri, 30 Aug 2024 15:47:39 +0000 Commentary by Philip Wegmann originally published by RealClearPolitics and RealClearWire Kamala Harris shrugged. Asked about former President Donald Trump’s questioning of her racial identity, the vice president replied, “Same old, tired playbook. Next question, please.” And then she laughed. “That’s it?” protested CNN’s Dana Bash, probing for more during the first sit-down interview with the … Full Article Commentaries Government Policies News
en Commentary: Mayors Can Combat Campus Unrest This School Year By deneenborelli.com Published On :: Tue, 10 Sep 2024 15:06:27 +0000 Commentary by Liz Katz originally published by RealClearPolitics and made available via RealClearWire As a Jewish parent whose daughter graduated from college in May, I witnessed firsthand the violent anti-Israel demonstrations and commencement cancellations that plagued campuses last academic year. While students are excited for their return this fall, I’m gripped by a sense of … Full Article Commentaries News
en Commentary: Second Assassination Attempt Forces New Reckoning for Trump, Secret Service By deneenborelli.com Published On :: Tue, 17 Sep 2024 14:23:52 +0000 Commentary by Susan Crabtree and Philip Wegmann originally published by The second assassination attempt against former President Donald Trump while he was golfing at one of his Florida courses on Sunday is forcing the United States Secret Service to further tighten security around the Republican presidential nominee and to reevaluate just how much Trump should … Full Article Commentaries News
en Commentary: Creating Jobs and Changing Lives – The Return of American Manufacturing By deneenborelli.com Published On :: Tue, 17 Sep 2024 14:33:22 +0000 Commentary by Maggie Miller was originally published by RealClearFlorida and RealClearWire In the heart of Riviera Beach, Florida, a company called K12 Print is redefining what it means to do business in America. This isn’t just about profits and productivity for John DiDonato, the CEO and founder. While financial success is part of the equation, … Full Article Commentaries News
en Commentary: The New Data on Migrant Crime By deneenborelli.com Published On :: Tue, 01 Oct 2024 20:15:36 +0000 Commentary by John R. Lott, Jr. originally published by RealClearPolitics and RealClearWire The new data on all the criminal noncitizens coming into the U.S. is shocking. The U.S. Immigration and Customs Enforcement (ICE) checks the background of illegal aliens they have in custody. But, the administration’s letter to Rep. Tony Gonzales (R-TX) shows that as … Full Article Commentaries Government Policies News
en Investigation: Waste of the Day – Florida Hospitals Spent $566 Million Caring for Illegal Immigrants By deneenborelli.com Published On :: Tue, 01 Oct 2024 20:25:26 +0000 Investigation by Jeremy Portnoy originally published by RealClearInvestigations and RealClearWire Topline: Florida hospitals spent $566 million providing care for illegal immigrants in the last six months of 2023, according to a recent report from the state’s Agency for Healthcare Administration. Key facts: Gov. Ron DeSantis passed a law in May 2023 instructing all hospitals that … Full Article Commentaries Government Policies News
en Investigation: Waste of the Day – New Yorkers Spend $25 Million on Ex-Governor’s Legal Troubles By deneenborelli.com Published On :: Tue, 08 Oct 2024 17:53:10 +0000 Investigation by Jeremy Portnoy originally published by RealClearInvestigations and RealClearWire Topline: The State of New York has spent $25.4 million to defend former Gov. Andrew Cuomo from sexual harassment lawsuits and criminal investigations over the last three years, The New York Times reported this month. Key facts: More than half of the money was spent … Full Article Commentaries News
en The singularity probability of a random symmetric matrix is exponentially small By www.ams.org Published On :: Wed, 16 Oct 2024 14:24 EDT Marcelo Campos, Matthew Jenssen, Marcus Michelen and Julian Sahasrabudhe J. Amer. Math. Soc. 38 (), 179-224. Abstract, references and article information Full Article
en On diffeomorphisms of even-dimensional discs By www.ams.org Published On :: Wed, 16 Oct 2024 14:24 EDT Alexander Kupers and Oscar Randal-Williams J. Amer. Math. Soc. 38 (), 63-178. Abstract, references and article information Full Article
en The existence of Zariski dense orbits for endomorphisms of projective surfaces By www.ams.org Published On :: Wed, 16 Oct 2024 14:24 EDT Junyi Xie J. Amer. Math. Soc. 38 (), 1-62. Abstract, references and article information Full Article
en A novel method produces native light-harvesting complex II aggregates from the photosynthetic membrane revealing their role in nonphotochemical quenching [Bioenergetics] By www.jbc.org Published On :: 2020-12-18T00:06:18-08:00 Nonphotochemical quenching (NPQ) is a mechanism of regulating light harvesting that protects the photosynthetic apparatus from photodamage by dissipating excess absorbed excitation energy as heat. In higher plants, the major light-harvesting antenna complex (LHCII) of photosystem (PS) II is directly involved in NPQ. The aggregation of LHCII is proposed to be involved in quenching. However, the lack of success in isolating native LHCII aggregates has limited the direct interrogation of this process. The isolation of LHCII in its native state from thylakoid membranes has been problematic because of the use of detergent, which tends to dissociate loosely bound proteins, and the abundance of pigment–protein complexes (e.g. PSI and PSII) embedded in the photosynthetic membrane, which hinders the preparation of aggregated LHCII. Here, we used a novel purification method employing detergent and amphipols to entrap LHCII in its natural states. To enrich the photosynthetic membrane with the major LHCII, we used Arabidopsis thaliana plants lacking the PSII minor antenna complexes (NoM), treated with lincomycin to inhibit the synthesis of PSI and PSII core proteins. Using sucrose density gradients, we succeeded in isolating the trimeric and aggregated forms of LHCII antenna. Violaxanthin- and zeaxanthin-enriched complexes were investigated in dark-adapted, NPQ, and dark recovery states. Zeaxanthin-enriched antenna complexes showed the greatest amount of aggregated LHCII. Notably, the amount of aggregated LHCII decreased upon relaxation of NPQ. Employing this novel preparative method, we obtained a direct evidence for the role of in vivo LHCII aggregation in NPQ. Full Article
en Russia–China defence and security relations: Insights from the expert community By www.chathamhouse.org Published On :: Wed, 31 Aug 2022 13:22:13 +0000 Russia–China defence and security relations: Insights from the expert community 13 September 2022 — 3:00PM TO 4:00PM Anonymous (not verified) 31 August 2022 Online Experts share insights on Russia–China military, defence, and security relations. In the defence and security realms, Russia–China relations resemble more pragmatic cooperation based on shared, calculated interests than an alliance. This event presents and discusses key findings from a recent expert survey conducted by Chatham House with the aim to gather insights on Russia–China military, defence, and security relations. Survey responses helped identify areas of bilateral cooperation but also crucial friction points and obstacles that prevent the relationship from developing further, as well as policy pathways for the West. Full Article
en Russia-China Defence and Security Relations By www.chathamhouse.org Published On :: Mon, 05 Sep 2022 11:16:50 +0000 Russia-China Defence and Security Relations This project explores the military and security linkages between Russia and China. jon.wallace 5 September 2022 The project seeks to provide a nuanced understanding of Russia-China relations in the defence and security realms. It also seeks to understand Russian political and security perceptions regarding Chinese inroads into what Russia considers its ‘near abroad’, as well as other sources of irritation and tension in the bilateral relationship. It offers recommendations to guide NATO policy responses. Full Article
en Beijing briefing: China’s wish for 2023? An end to lockdown By www.chathamhouse.org Published On :: Tue, 29 Nov 2022 16:12:17 +0000 Beijing briefing: China’s wish for 2023? An end to lockdown The World Today mhiggins.drupal 29 November 2022 Xi Jinping will try to beef up Global South relations in 2023, but weary Chinese and the business sector need pandemic restrictions to end, says Yu Jie. This past year in Chinese politics was capped by a highly anticipated 20th Party Congress in Beijing in October which marked the beginning of General Secretary Xi Jinping’s unprecedented third term. Xi stacked the all-powerful Politburo with male loyalists and left the rest of the world to sift through the implications of his leadership reshuffle. Meanwhile, Xi made headlines at the G20 summit in Indonesia, maintaining a largely positive tone with President Joe Biden and being caught on camera chiding Canada’s Justin Trudeau for leaks after their talks. So, what can we expect Xi to pull out of the hat in 2023, the Chinese Year of the Rabbit? In February and March, the conversations around Beijing dinner tables will focus on the composition of the new central government – the important seats within the Chinese State Council. This body must deliver Xi’s ‘security-oriented’ economic agenda as well as his much-promoted ‘Common Prosperity’ initiative. The ‘Zero-Covid’ strategy has exacerbated youth unemployment and tested the patience of China’s upwardly mobile middle-class Pundits will be paying particular attention to who is put in charge of economic planning, who the new foreign minister might be and who will govern the central bank. I will offer my reading of these tea leaves as the year unfolds. In April and May, speculation may turn to whether China will finally open its borders to foreign visitors and those compatriots who want to be reunited with loved ones after enduring the pandemic lockdown. While some loosening of restrictions began in November 2022, China is still balancing its twin aims of containing the spread of Covid and re-engineering its economy along similar lines to Europe. Beijing’s controversial ‘Zero-Covid’ strategy has intensified economic pressures, exacerbated rising levels of youth unemployment and tested the patience of China’s middle class, which has led to unprecedented civil disobedience. Those not employed by the state have been hit particularly hard. It is difficult to see how China’s economy can crank up again until Beijing reduces its internal restrictions and reconnects with the world. A pivot to the Global South June and July will be prime season for Beijing’s diplomacy with the Global South. Xi recently announced that China would host the third Belt and Road Forum in 2023 – a meeting of heads of states from predominantly developing countries to discuss his flagship foreign affairs initiative. As seen in the Political Report of the 20th Party Congress, Xi has abandoned the ‘new type of great power relations’ language he previously used to describe relations with the US-led western world. In its place, Xi is stressing that China should develop its ties with the Global South through his Global Development and Global Security initiatives which were announced in 2021 and 2022 respectively. These aim to reshape the global governance agenda in multilateral forums and project Beijing’s influence on to the developing world. Sport will dominate throughout August and September. While Beijing’s spending spree on football promotion might have failed to see the national side qualify for the World Cup, its table tennis team has proved invincible and continues to cheer up the nation. I will offer my own verdict on why there is such a stark contrast between the success of the two men’s teams and explain the meaning of sports in modern Chinese society. In October and November, young graduates will begin their careers while new university students start to arrive on campus. Chinese students are constantly subjected to strenuous testing. And like their western peers, they face the inevitable pressures of finding a job, repaying the mortgage and other everyday facts of life. The burnout of China’s Generation Z The term ‘involution’ – neijuan – has been adopted by China’s Generation Z to describe their feelings of burnout at the ever-increasing expectations associated with high performance. Equally, they have strong opinions about their own government as well as western liberal democracies. China’s leaders of the future will come from their ranks so it will be worthwhile spending some time trying to understand what makes them tick. Billions will want a return to normal life without the fear of having the wrong colour – yellow or red – on their Covid health QR code Whatever the Year of the Rabbit holds for China, billions of its people will want to have their life return to normal without the fear of having the wrong colour – yellow or red – on their Covid health QR code. Only a green code shows a person is healthy and free to move around. The Covid threat has hovered over people for three years and as borders reopen, they may be holding their breath. A slowing economy dimming consumer confidence and a precarious international environment make it look even harder for President Xi to pursue the agenda outlined in October 2022. As the Year of the Rabbit dawns, China doesn’t need a Mad Hatter or a March Hare, instead it urgently needs a sound path to economic recovery and a plan to reopen its borders that works for everyone. Full Article
en China’s zero-COVID cannot continue, reopening is needed By www.chathamhouse.org Published On :: Thu, 01 Dec 2022 11:16:46 +0000 China’s zero-COVID cannot continue, reopening is needed Expert comment NCapeling 1 December 2022 Unsure of how to loosen rules, local officials have doubled down on severe restrictions behind so much economic damage. Reopening will quell public anger. When President Xi Jinping was seen unmasked at the G20 summit in Indonesia, he maintained a largely positive tone with President Joe Biden and other world leaders. This left an impression that China was on the verge of withdrawing its zero-COVID strategy. A set of loosening policy measures introduced by Beijing seemed to further suggest that China was on track to reopen. As outlined in the 20th party congress, Xi wants to forge a pathway towards economic modernization and this means building economic resilience and a further increase in household incomes. However, a series of displays of public defiance against the government’s zero-COVID policy has left the rest of the world perplexed. Some loosening of restrictions announced on 11 November by the central government, with the number of new cases still rising rapidly, left the provincial governments in confusion as to which direction to turn. Absolute political loyalty The persistent slogan of ‘zero-COVID’ was in stark contrast to the slackening of restrictions. Local officials decided to double down on stringent COVID measures as a way of displaying absolute political loyalty to the top, which inevitably caused daily agonies among large parts of the Chinese population. The strategy has intensified economic pressures, exacerbated rising levels of youth unemployment, and tested the patience of the entire country This decision-making process sheds some light on the way the Chinese bureaucracy approaches crises at a time when the party leadership is tightening political control. Lower-level officials avoided making important decisions and instead decided to wait for instructions from the top. As the rules were unclear, they implemented policies according to past precedents, in this case zero-COVID, which had worked relatively well for China in 2020 and 2021. For decades, local governments have been major political actors in China and have known what works best under local conditions. But with tighter regulation being exercised by lower-level bureaucrats and civil servants, there is less opportunity for the input of local knowledge, increasing the risk of ineffective policies being implemented. Away from Beijing, those not employed by the state have been hit particularly hard by zero-COVID measures. The strategy has intensified economic pressures, exacerbated rising levels of youth unemployment, and tested the patience of the entire country. It is difficult to see how China’s economy can crank up again until the country reduces its internal restrictions and reconnects with the world Billions of Chinese people want to have their life return to normal without the fear of having the wrong colour – yellow or red – on their COVID health QR code or endless mandatory testing. Only a green code shows that a person is healthy and able to move around freely. Economy is suffering China is still balancing its twin aims of containing the spread of COVID and re-engineering its economy along similar lines to the rest of the world. It is difficult to see how China’s economy can crank up again until the country reduces its internal restrictions and reconnects with the world. Full Article
en Director's briefing: Key challenges for China’s economy in 2023 By www.chathamhouse.org Published On :: Wed, 18 Jan 2023 16:47:15 +0000 Director's briefing: Key challenges for China’s economy in 2023 6 February 2023 — 8:00AM TO 9:15AM Anonymous (not verified) 18 January 2023 Chatham House This event examines the structural challenges facing the Chinese economy in the wake of the 20th National Congress of the Chinese Communist Party. This event examines the structural challenges facing the Chinese economy after the 20th National Congress of the Chinese Communist Party in October 2022 and how President Xi Jinping is responding to short and long-term domestic pressures. The panel, including Professor Huang Yiping, discuss how quickly the Chinese economy could rebound after the Chinese government abandoned its ‘Zero COVID-19’ policy in December 2022 and to what extent the Chinese economy is pivoting toward Xi Jinping’s stated goal of ‘self-reliance’. The panel also discuss the broader implications for the global economy. Key questions to be explored: Which sectors will China prioritize in pursuit of greater economic self-reliance? If China is turning inward, how will it drive technological innovation in the coming years? Is China’s economy robust enough to withstand geopolitical turbulence and other external shocks? This event is held under the Chatham House Rule. Full Article
en Analysis of {beta}-lactone formation by clinically observed carbapenemases informs on a novel antibiotic resistance mechanism [Enzymology] By www.jbc.org Published On :: 2020-12-04T00:06:05-08:00 An important mechanism of resistance to β-lactam antibiotics is via their β-lactamase–catalyzed hydrolysis. Recent work has shown that, in addition to the established hydrolysis products, the reaction of the class D nucleophilic serine β-lactamases (SBLs) with carbapenems also produces β-lactones. We report studies on the factors determining β-lactone formation by class D SBLs. We show that variations in hydrophobic residues at the active site of class D SBLs (i.e. Trp105, Val120, and Leu158, using OXA-48 numbering) impact on the relative levels of β-lactones and hydrolysis products formed. Some variants, i.e. the OXA-48 V120L and OXA-23 V128L variants, catalyze increased β-lactone formation compared with the WT enzymes. The results of kinetic and product studies reveal that variations of residues other than those directly involved in catalysis, including those arising from clinically observed mutations, can alter the reaction outcome of class D SBL catalysis. NMR studies show that some class D SBL variants catalyze formation of β-lactones from all clinically relevant carbapenems regardless of the presence or absence of a 1β-methyl substituent. Analysis of reported crystal structures for carbapenem-derived acyl-enzyme complexes reveals preferred conformations for hydrolysis and β-lactone formation. The observation of increased β-lactone formation by class D SBL variants, including the clinically observed carbapenemase OXA-48 V120L, supports the proposal that class D SBL-catalyzed rearrangement of β-lactams to β-lactones is important as a resistance mechanism. Full Article
en Mutation of an atypical oxirane oxyanion hole improves regioselectivity of the {alpha}/{beta}-fold epoxide hydrolase Alp1U [Enzymology] By www.jbc.org Published On :: 2020-12-11T00:06:20-08:00 Epoxide hydrolases (EHs) have been characterized and engineered as biocatalysts that convert epoxides to valuable chiral vicinal diol precursors of drugs and bioactive compounds. Nonetheless, the regioselectivity control of the epoxide ring opening by EHs remains challenging. Alp1U is an α/β-fold EH that exhibits poor regioselectivity in the epoxide hydrolysis of fluostatin C (compound 1) and produces a pair of stereoisomers. Herein, we established the absolute configuration of the two stereoisomeric products and determined the crystal structure of Alp1U. A Trp-186/Trp-187/Tyr-247 oxirane oxygen hole was identified in Alp1U that replaced the canonical Tyr/Tyr pair in α/β-EHs. Mutation of residues in the atypical oxirane oxygen hole of Alp1U improved the regioselectivity for epoxide hydrolysis on 1. The single site Y247F mutation led to highly regioselective (98%) attack at C-3 of 1, whereas the double mutation W187F/Y247F resulted in regioselective (94%) nucleophilic attack at C-2. Furthermore, single-crystal X-ray structures of the two regioselective Alp1U variants in complex with 1 were determined. These findings allowed insights into the reaction details of Alp1U and provided a new approach for engineering regioselective epoxide hydrolases. Full Article
en Structure, mechanism, and regulation of mitochondrial DNA transcription initiation [Enzymology] By www.jbc.org Published On :: 2020-12-25T00:06:31-08:00 Mitochondria are specialized compartments that produce requisite ATP to fuel cellular functions and serve as centers of metabolite processing, cellular signaling, and apoptosis. To accomplish these roles, mitochondria rely on the genetic information in their small genome (mitochondrial DNA) and the nucleus. A growing appreciation for mitochondria's role in a myriad of human diseases, including inherited genetic disorders, degenerative diseases, inflammation, and cancer, has fueled the study of biochemical mechanisms that control mitochondrial function. The mitochondrial transcriptional machinery is different from nuclear machinery. The in vitro re-constituted transcriptional complexes of Saccharomyces cerevisiae (yeast) and humans, aided with high-resolution structures and biochemical characterizations, have provided a deeper understanding of the mechanism and regulation of mitochondrial DNA transcription. In this review, we will discuss recent advances in the structure and mechanism of mitochondrial transcription initiation. We will follow up with recent discoveries and formative findings regarding the regulatory events that control mitochondrial DNA transcription, focusing on those involved in cross-talk between the mitochondria and nucleus. Full Article
en The endosomal trafficking regulator LITAF controls the cardiac Nav1.5 channel via the ubiquitin ligase NEDD4-2 [Computational Biology] By www.jbc.org Published On :: 2020-12-25T00:06:30-08:00 The QT interval is a recording of cardiac electrical activity. Previous genome-wide association studies identified genetic variants that modify the QT interval upstream of LITAF (lipopolysaccharide-induced tumor necrosis factor-α factor), a protein encoding a regulator of endosomal trafficking. However, it was not clear how LITAF might impact cardiac excitation. We investigated the effect of LITAF on the voltage-gated sodium channel Nav1.5, which is critical for cardiac depolarization. We show that overexpressed LITAF resulted in a significant increase in the density of Nav1.5-generated voltage-gated sodium current INa and Nav1.5 surface protein levels in rabbit cardiomyocytes and in HEK cells stably expressing Nav1.5. Proximity ligation assays showed co-localization of endogenous LITAF and Nav1.5 in cardiomyocytes, whereas co-immunoprecipitations confirmed they are in the same complex when overexpressed in HEK cells. In vitro data suggest that LITAF interacts with the ubiquitin ligase NEDD4-2, a regulator of Nav1.5. LITAF overexpression down-regulated NEDD4-2 in cardiomyocytes and HEK cells. In HEK cells, LITAF increased ubiquitination and proteasomal degradation of co-expressed NEDD4-2 and significantly blunted the negative effect of NEDD4-2 on INa. We conclude that LITAF controls cardiac excitability by promoting degradation of NEDD4-2, which is essential for removal of surface Nav1.5. LITAF-knockout zebrafish showed increased variation in and a nonsignificant 15% prolongation of action potential duration. Computer simulations using a rabbit-cardiomyocyte model demonstrated that changes in Ca2+ and Na+ homeostasis are responsible for the surprisingly modest action potential duration shortening. These computational data thus corroborate findings from several genome-wide association studies that associated LITAF with QT interval variation. Full Article
en Islamic radicalisation in Central Asia By www.chathamhouse.org Published On :: Tue, 20 Aug 2024 15:47:13 +0000 Islamic radicalisation in Central Asia 18 September 2024 — 10:00AM TO 11:30AM Anonymous (not verified) 20 August 2024 Chatham House and Online Experts analyse rising foreign recruitment, current counter-radicalisation efforts and challenge past Chatham House predictions made of the Central Asia region. In 2014, Chatham House released a report titled The Myth of Post-Soviet Muslim Radicalisation in the Central Asian Republics. At that time, a significant, Western-funded counter-radicalisation industry was addressing what was believed to be a major issue of Islamic violent extremism in the region.Over the past decade, two key developments have occurred. First, Western interest in the region has declined in favour of traditional geopolitical concerns. Second, while violent extremism incidents within Central Asia remain rare, there has been a notable recruitment of Central Asians by foreign groups, with several attacks carried out by Central Asians overseas.In this event, the original report’s authors, John Heathershaw and David Montgomery, will discuss whether the report’s arguments are still valid and how we might answer these questions today.This raises several questions:What did the report accurately predict, and where did it fall short?How can we explain the lack of violent extremist organisations (VEOs) within Central Asia but the presence of Central Asians in VEOs abroad?Could repression by Central Asian states both domestically and transnationally have contributed to this issue?What, if anything, can the counter-radicalisation industry do today to address this problem more effectively?Please note that in-person places are limited. Please wait for confirmation before participating. Full Article
en Director’s Briefing: Assessing foreign policy challenges for the next US president By www.chathamhouse.org Published On :: Tue, 27 Aug 2024 16:37:13 +0000 Director’s Briefing: Assessing foreign policy challenges for the next US president 5 September 2024 — 2:00PM TO 3:00PM Anonymous (not verified) 27 August 2024 Chatham House and Online This briefing will explore what challenges might await the winner of 2024 US presidential election. As the 2024 US Presidential election draws closer, the future direction of American foreign policy seems ever more uncertain. Kamala Harris, the Democratic presidential candidate, appears to be embracing many of Biden’s policies, but she brings a different background, and most likely a different team, so change is likely. Donald Trump has more well-known views on foreign policy, but the context for a second Trump administration would be very different than the first.The next U.S. President will be confronted a world in need of leadership with two major wars, a more assertive and capable China, a climate crisis, ungoverned technological change, emerging powers that demand a seat at the table, and debt distress across much of the developing world.Please join us for this critical conversation covering:How will US-China strategic competition and the threat of conflict over Taiwan challenge US policy makers?What are the risks and challenges posed by Russia’s illegal full-scale invasion of Ukraine?How does war in the Middle East and the threat of regional escalation shape US foreign policy? Full Article
en How can young people in MENA thrive despite economic and political insecurity? By www.chathamhouse.org Published On :: Fri, 30 Aug 2024 12:47:13 +0000 How can young people in MENA thrive despite economic and political insecurity? 24 September 2024 — 2:00PM TO 3:00PM Anonymous (not verified) 30 August 2024 Online Experts share insights on how young people navigate challenges and find opportunities in a changing domestic and regional landscape. Across the Middle East and North Africa young people between the ages of 15 and 29 comprise around 24 per cent of the population in the region. As the complex regional geopolitical developments unfold, the majority of these young people are living in a time of economic and political insecurity, with many, such as Iraqis and Libyans, also growing up during conflict and uncertainty.Enhanced education and employment programs are key opportunities for development and stability in the region. Despite this, limited resources and competing priorities have meant that governments often struggle to deliver competitive educational and employment opportunities and lack the capacity and funding for education reform and active labour market policy development. Key tensions that pit modernity and autonomy against tradition and control continue to frame the education and skills development landscape.This webinar will address:The challenges young people within the MENA region face in different contexts;The role education and employment play in developing skills for 21st century challenges;Spaces for young people to practice citizenship and participate in political processes;Youth’s economic prospects while navigating the tumultuous backdrop of enduring conflict and authoritarianism. Full Article
en Making the circular economy work for global development: how the UN Summit of the Future can deliver By www.chathamhouse.org Published On :: Thu, 05 Sep 2024 09:02:13 +0000 Making the circular economy work for global development: how the UN Summit of the Future can deliver 23 September 2024 — 6:15PM TO 9:00PM Anonymous (not verified) 5 September 2024 Online This policy roundtable focusses on how to advance implementation of a global approach and collaboration to an inclusive circular economy for an updated post-2030 SDG framework. As the world looks beyond the Sustainable Development Goals (SDGs) towards the post-2030 era, integrating the principles of the circular economy into the future global development agenda becomes imperative. A new Chatham House research paper about the role of the circular economy for the SDGs and their possible extension into the post-2030 development agenda will be launched in September 2024 during the UN Summit of the Future and the New York Climate Week. The paper outlines the rationale for the integration of circular economy goals into for the next crucial phase of international development and how to meet net-zero 2050 targets. It provides actionable recommendations on international cooperation mechanisms for policymakers and stakeholders at the UN Summit for the Future in 2024 and beyond.The aim of the roundtable is to bring together stakeholders and leaders from intergovernmental organisations, business, governments and civil society. The focus of the roundtable meeting is: Reflections on the Summit of the Future and the role of circularity for an updated post-2030 SDG framework.Discussion on key aspects of the institutional arrangements and international coordination that are needed for a globally coordinated approach to achieve an inclusive circular economy that supports SDG implementation. Development of joint strategies on how to advance implementation of a global approach and collaboration to an inclusive circular economy as a follow-up from the Summit of the Future.The objective is to emerge from the roundtable with a clearer roadmap for translating the recommendations for international coordination into concrete actions, with a shared commitment to driving meaningful change on the international level.The event is co-hosted by Chatham House and partners from the Global Circular Economy Roadmap initiative including the African Circular Economy Network, the African Development Bank, Circular Change, Circular Innovation Lab, Circle Economy, EU CE Stakeholder Platform, Hanns Seidel Foundation, Institute of Global Environmental Strategies, Sitra, UNIDO, World Business Council on Sustainable Development, World Economic Forum and the Wyss Academy for Nature.Further background information is available on the initiative website.More speakers to be announced. Full Article
en Labour Party Conference – International affairs networking brunch By www.chathamhouse.org Published On :: Mon, 16 Sep 2024 10:32:13 +0000 Labour Party Conference – International affairs networking brunch 24 September 2024 — 11:00AM TO 12:00PM Anonymous (not verified) 16 September 2024 Labour – Grace 1 Suite, Hilton Hotel, Liverpool Hosted by Sir Simon Fraser, Chairman of Chatham House. This event is taking place at the Hilton Hotel, Grace 1 Suite, Liverpool. You are warmly invited to join us for an exclusive networking brunch at the 2024 Labour Party Conference.Sir Simon Fraser, Chairman of Chatham House, and Olivia O’Sullivan, Director of Chatham House’s UK in the World Programme, will deliver opening remarks, followed by an interactive networking session where you will have the chance to engage with a wide range of stakeholders. Join us to connect with key senior parliamentary, corporate and media attendees at the Labour Party Conference.This event will be taking place outside of the secure zone. Full Article
en The 2024 US Election: Global Consequences By www.chathamhouse.org Published On :: Tue, 17 Sep 2024 14:32:14 +0000 The 2024 US Election: Global Consequences 26 September 2024 — 5:30PM TO 6:45PM Anonymous (not verified) 17 September 2024 Online This webinar explores what is at stake for US policy in key regions around the globe. In the lead up to the 2024 US presidential election, Chatham House experts discuss:What is at stake for US policy towards China, Europe, and Latin America and what challenges will confront the next US president?Can the US lead when its partners and allies are continually dissatisfied by the new turn in its trade and economic policy?What are the core humanitarian challenges and how can they be addressed? Full Article
en US domestic polarization and implications for the presidential election By www.chathamhouse.org Published On :: Thu, 19 Sep 2024 09:07:13 +0000 US domestic polarization and implications for the presidential election 30 September 2024 — 5:00PM TO 6:00PM Anonymous (not verified) 19 September 2024 Online This webinar explores the rise of polarization amongst the US public and implications for the upcoming election. Domestic polarization has been on the rise in the US in recent years, with Democrats and Republicans moving further away from the centre on key policy issues. As polarization increases, so too do concerns about the state of US democracy and prospects of political violence surrounding the upcoming presidential election. This expert panel discusses key questions including:What are the driving forces of the growing political divide in the US?How far apart are voters on key election issues, from healthcare to immigration to national security?How is partisan polarisation altering the effectiveness of US foreign policy and influencing America’s role in the world? Full Article
en Business Briefing: US election geopolitical and economic risk scenarios By www.chathamhouse.org Published On :: Mon, 23 Sep 2024 12:07:13 +0000 Business Briefing: US election geopolitical and economic risk scenarios 10 October 2024 — 11:45AM TO 1:00PM Anonymous (not verified) 23 September 2024 Chatham House Please join us for this critical discussion of the US Election related global business risks. The outcome of the US presidential election will have significant, intersecting implications for global as well as American business. At stake will be the degree of continuity and stability on both the domestic and international fronts, with a Harris presidency pursuing policies building broadly on the Biden Administration and a second Trump Administration departing sharply from them—with both shaped and limited by control of Congress.An already volatile geopolitical environment and global economy may become even more unpredictable in the face of potential American political instability and uncertain leadership in the international community.Please join us for this critical session to discuss:How might trade policy differ between a Harris and Trump presidency? Will national security pressures, especially over China, lead to greater policy commonalities than expected?What might differing approaches to decarbonization and the energy transition mean for the future of policy toward EVs, critical mineral supply chains, and ‘green’ industrial subsidy?How might each Administration approach fiscal policy? Will either push for a tightening to the current loose policy—and what may be implications for US debt and the dollar?What economic effects can we expect in the case of a disputed election result or non-peaceful transfer of power—and will Corporate America be compelled to make public statements? Full Article
en Looking ahead to the 2024 US Presidential Election By www.chathamhouse.org Published On :: Mon, 23 Sep 2024 18:07:13 +0000 Looking ahead to the 2024 US Presidential Election 2 October 2024 — 6:00PM TO 7:00PM Anonymous (not verified) 23 September 2024 Chatham House and Online This panel explores the state of the US presidential election following the vice presidential debate. With only weeks to go, this panel looks at the state of the 2024 US presidential election.Harris and Trump are campaigning to drive up voter enthusiasm and bring undecided voters to their side.What are the key issues shaping voter’s preferences and how may this impact voter turnout? What can we expect in the weeks ahead? Full Article
en Security and defence 2025 By www.chathamhouse.org Published On :: Thu, 26 Sep 2024 13:42:14 +0000 Security and defence 2025 6 March 2025 — 8:00AM TO 6:00PM Anonymous (not verified) 26 September 2024 Chatham House and Online Join senior policymakers, business leaders and international security experts at Chatham House for a day of thought-provoking discussions exploring the tools needed to equip military forces with for an increasingly insecure future. You will receive further instructions regarding your registration soon. Future-proofing a fracturing worldThe security and defence landscape is characterised by rapid change and evolving threats to global stability. The uncertainties of a complex geopolitical environment, with war on multiple fronts, are exacerbated by the array of threats presented across the domains of conflict - from land, sea and air through to space and technology. From drone swarm attacks in the Donbas, utilising the latest battlefield technology to gain advantages on the land and in the air, through to the battle for primacy in the space domain above the strategically important, yet inhospitable Arctic shipping lanes, the domains of conflict are becoming increasingly intertwined. A holistic strategy fit to tackle the threats of a fracturing world will rely on effective collaboration between the public and private sectors.The 2025 Security and Defence conference brings together global policymakers, senior military and armed forces figures, business leaders and civil society experts. An insightful day of keynotes, high-level panel dialogues, and ‘under-the-Rule’ spotlights will explore how advanced technology is shaping the battlefield, examine how security architectures can be redesigned to support the end goal of peace, and address the future of arms control measures. Discussions will also unpack how forward-thinking defence actors might unlock fresh opportunities alongside current challenges. Forging stronger ties with partners to future-proof institutions and develop the architecture that will sustain security and defence ecosystems into the future will be crucial to ensuring that fighting forces are equipped, not just for the conflicts of today, but the security challenges of tomorrow.Why attend?Learn more about the areas that leaders across the security and defence ecosystem are prioritizing and how they aim to counter emerging threats in order to foster resilience in this environment.Gain an understanding of the array of threats that are present, both across the domains of conflict and also the different regions of the world.In-person networking opportunities with senior leaders from across the government, international militaries and global business, as well as access to exclusive ‘under-the-Rule’ sessions.The institute occupies a position of respect and trust, and is committed to fostering inclusive dialogue at all events. Event attendees are expected to uphold this by adhering to our code of conduct. Full Article
en What are MENA countries’ priorities for climate action and COP? By www.chathamhouse.org Published On :: Wed, 02 Oct 2024 08:47:13 +0000 What are MENA countries’ priorities for climate action and COP? 24 October 2024 — 1:00PM TO 2:00PM Anonymous (not verified) 2 October 2024 Online Experts discuss the impacts of COP27 and 28 on the region and priorities for COP29. In November this year, all eyes will be on Azerbaijan as Baku hosts the 29th UN Climate Conference (COP). Hosting COP offers an opportunity to push for climate action that reflects the host country’s unique circumstances, as well as regional interests. COP28 in the United Arab Emirates and COP27 in Egypt highlighted the climate priorities for the Middle East and North Africa (MENA), one of the world’s most vulnerable regions to the impacts of climate change.From rising temperatures and deadly heatwaves, to extreme weather events such as heavy rainstorms, sandstorms, and cyclones, climate change exacerbates existing vulnerabilities. These vulnerabilities include water scarcity, food security, and the preservation of livelihoods increasingly threatening the region’s social and economic stability.While there has been some progress in advancing formal negotiations on key issues, the gap between COP agreements and tangible actions to address the increasingly severe effects of climate change in the MENA region remains vast. This webinar aims to assess:What are the key tangible impacts of COP27 and COP28 in Egypt and the UAE respectively on driving climate action in the region?How do MENA countries balance COP28’s landmark agreement to transition away from fossil fuels with their unique national circumstances?How will the region benefit from COP28 breakthrough in integrating the issues of climate, peace and security in climate action through the ‘Declaration of Climate, Relief, Recovery and Peace’?What are the priorities for COP29 and how do they align with the climate agenda in the MENA region? Full Article
en Iraq Initiative Conference 2024 By www.chathamhouse.org Published On :: Fri, 04 Oct 2024 10:12:13 +0000 Iraq Initiative Conference 2024 20 November 2024 — 9:30AM TO 5:00PM Anonymous (not verified) 4 October 2024 Chatham House and Online Our sixth annual Iraq Initiative conference will convene Iraqi and international policymakers, experts and civil society in London to discuss the critical questions for Iraq’s future, and the country’s trajectory in the year ahead. Over two decades following regime change, Iraq appears to be on a path to recovery and growth. With the support of high oil prices, Prime Minister Mohammed Shia’ al-Sudani’s government is implementing an ambitious plan to develop critical infrastructure, enhance ties with neighbouring countries, and renegotiate relationships with international partners like the US and UN. The political system has rebounded from a series of shocks and is in the process of consolidating and centralising power in Baghdad under the governing Shia Coordination Framework.However, underlying political, economic, security, and regional challenges, if not addressed, threaten this apparent stability and frustrate longer-term hope for a better future. Despite the country’s wealth, many young Iraqis struggle to find jobs while the space for civic activism shrinks. Meanwhile, the escalation of violence across the region threatens to engulf Iraq in further conflict. With the 2025 parliamentary election approaching, infighting within the governing coalition also suggests political turmoil in the year ahead.The key questions explored through each session include:How can the Sudani government tackle the roots of these obstacles to ensure that development can be sustained over time?What are the key steps to coherent and effective state-building?And how can regional and international actors support this process?This conference is part of Chatham House’s Iraq Initiative.This year’s conference features RE-PLAY – a video art exhibition curated by Dr Tamara Chalabi of the Ruya Foundation. The artists featured in this exhibition, spanning multiple generations both within Iraq and in the Iraqi diaspora, use their work to grapple with complex notions of Iraqi identity through the thematic issues of heritage, exile, disconnection, freedom of expression, and other persistent issues explored in this show.Simultaneous English-Arabic interpretation will be available for all conference sessions. This event is part of Chatham House’s Iraq Initiative. Iraq Initiative conference 2024 agenda [English] (PDF, 2.97MB) Iraq Initiative conference 2024 agenda [Arabic] (PDF, 2.29MB) RE-PLAY Video Art Exhibition [English] (PDF, 0.23MB) RE-PLAY Video Art Exhibition [Arabic] (PDF, 0.22MB) The institute occupies a position of respect and trust, and is committed to fostering inclusive dialogue at all events. Event attendees are expected to uphold this by adhering to our code of conduct. Full Article
en Civilian priorities for ending the war in Sudan By www.chathamhouse.org Published On :: Tue, 08 Oct 2024 08:17:13 +0000 Civilian priorities for ending the war in Sudan 31 October 2024 — 12:30PM TO 1:30PM Anonymous (not verified) 8 October 2024 Chatham House and Online Dr Abdalla Hamdok, former prime minister of Sudan and chairperson of the Coordination of Civil Democratic Forces (Tagadum), will discuss the prospects for conflict resolution in Sudan. The intensification of fighting across Sudan demonstrates that both warring parties and their allies are continuing to pursue an outright military victory in the war, with little regard for the human cost. Diplomatic efforts, including the US-led Geneva talks in August, have produced little progress towards a ceasefire. Meanwhile, the conflict has resulted in widespread devastation, mass displacement, and an escalating hunger crisis.Civilian perspectives on ending the war, their vital efforts in providing lifesaving humanitarian assistance, and the aspiration of many Sudanese for a more inclusive and democratic Sudan, have been sidelined by the interests and actions of the belligerent parties.At this event, Dr Abdalla Hamdok will discuss options for reaching a ceasefire and seeking a peaceful political resolution to the conflict. He will also address the urgent need for an international response to what has become the world’s largest humanitarian crisis.Please note that in-person attendance for this event will be balloted. Guests looking to join in-person must ‘register interest’ in joining. Full confirmations to successful applicants will be sent on Monday 28 October at 15:00.Individual membership provides you with the complete Chatham House experience, connecting you with a unique global policy community. Find out more about membership. Full Article