About a thousand people stood solemnly in the rain at a cenotaph in downtown Halifax on Monday morning to pay tribute to veterans on Remembrance Day.

Some Nova Scotia municipal leaders are defending a funding stream the federal Conservatives say they will cut if elected, calling it a "game changer."

Building highways isn't the only solution to the growing problem of traffic congestion in and around the Halifax Regional Municipality, says the head of an organization that advocates for rail-based public transit.

The new mayor and council are planning big changes for the Cape Breton Regional Municipality, and one of those is at the top of the staff organizational chart now that chief administrative officer Marie Walsh is set to retire.

Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

The ancient burial structures, strikingly similar all across Europe and the mediterranean, have puzzled scientists and historians for centuries

In a new biopic starring Kate Winslet, Miller's many lives—as an artist, model, muse, cook and war correspondent—need little embellishment

Three weeks after the death of another beluga whale at Marineland, the Ontario government is speaking publicly about its ongoing investigation of the park, saying water troubles are under control after a recent investment.

Benjamin Mora, a finalist for Canadian Premier League coach of the year, has left York United FC after five months and 22 matches at the helm.

Every November, Jan Diehl adorns her Martensville, Sask., home with a special Remembrance Day tribute.

After playing her first season with the WNBA's Washington Mystics and representing Team Canada at the Olympics, forward Aaliyah Edwards held her second annual "You Got Knext" camp in her hometown over the weekend.

A recent request to change the wording in a mandatory Indigenous intercultural course for lawyers in British Columbia led to a debate over whether the changes amounted to residential school denialism. 

Marc-André Fleury stopped 26 shots in what will likely be his final game in Pittsburgh as the Minnesota Wild beat the Penguins 5-3 on Tuesday night. He spent 13 seasons with Pittsburgh and won Stanley Cups in 2009, 2016 and 2017.

Winnipeg Jets goaltender Connor Hellebuyck, Colorado Avalanche centre Nathan MacKinnon and Buffalo Sabres goaltender Ukko-Pekka Luukkonen have been named the NHL's three stars of the week.

It's not a complete surprise that curling and other sports have been cancelled because of the COVID-19 pandemic, but it doesn't make it any easier on teams that had their hopes of representing Northern Ontario at a national curling level dashed.

CME Group, the world's leading derivatives marketplace, today announced its new CBL Australian Carbon Credit Unit (ACCU) futures have launched and are available for trading. A total of five...

CRISPR–Cas9 has revolutionized gene editing for traditional and nontraditional model organisms alike. This tool has opened the door to new mechanistic studies of basic mosquito biology as well as the development of novel vector control strategies based on CRISPR–Cas9, including gene drives that spread genetic elements in the population. Although the promise of the specificity, flexibility, and ease of deployment CRISPR is real, its implementation still requires empirical optimization for each new species of interest, as well as to each genomic target within a given species. Here, we provide an overview of designing and testing single-guide RNAs for the use of CRISPR-based gene editing tools.

New Licensing, Support Programs For Certification Accompany Software Upgrades

SolidWorks CAD Software Enables Engineering Students to Fit ‘CubeSat’ Components Into a Four-Inch Square Box

New design increases user leverage and mobility, and keeps hands clean

Team gears up for the America's Cup in 2007 by creating SolidWorks 3D models to gain higher performance from almost every aspect of the racing yacht

3D CAD software helps make inventors' concepts a reality in contest for $1 million prize on popular ABC TV show

Extensive automation of time-consuming tasks gives designers fast, accurate tools for testing their ideas

SolidWorks Design, Simulation, and Publishing Software Contribute to Halving of Design Cycle, 20 Percent Materials Saving, and Paperless Assembly

Cutting days and weeks from design cycle gives Syncroness more time to focus on designing lightweight, high performance products

Automation of time-consuming analysis tasks and support for popular CAD programs gives designers and engineers a safety net for experimenting with new product designs

Magicians' equipment supplier creates products that mystify even the most experienced illusionists

U.K. company reduces time, errors, and costs of designing custom Victorian greenhouses in 3D CAD software

ModernTech Provides Audio Electronics Manufacturer with Design and Communication Tools to Bring Industry-Leading Speakers to Market

Australia's prime minister on Thursday vowed to ban children under 16 from social media, saying the pervasive influence of platforms like Facebook and TikTok was "doing real harm to our kids".

In August, 80 Somali believers met outside of Örebro, Sweden, for a week of fellowship, encouragement and teaching. Eight new believers were baptised.

Young Somali believers take bold risks in sharing their faith with their families and community.

No matter people’s struggles, the counselling ministry focuses on a right relationship with God as the beginning of the healing process.

OM short-term workers visit a Muslim family and enjoy food and conversation, which they hope will help lead the family to the truth.

From 21 July - 19 August, Logos Hope brought the hope of the Gospel to over 50,000 people who visited the ship in Incheon, South Korea.

The story of how OM’s Ship Ministry began

Cycling to reach people in remote villages in Malawi, Yolanda Mamvura experienced a puncture which led her to pray for an epileptic.

As the women's team goes out to pray and encourage believers, a woman in hospital and a young man experience Him first hand.

As OM teams spread out across the nation last month, bringing news of God and His love, so the stories return of people hungry and thirsty for His truth.

Teams help nominal Christian believers understand the truth of God's love for them so that they will worship Him instead of idols.

Going house to house to share the Good News, a team discovers a believing family who had been isolated and out of Christian fellowship for eight years.

OM supported the Ukrainian Military Ministry in order to print trauma counselling books

Zosia and Monika couldn’t find an addiction therapist within a 60 kilometre radius of their town, so they became therapists themselves.

A local Chinese mother teaches Cantonese to three Pakistani mothers before they all watch the film 'Magdalena: Released from Shame' together.

An update about the flood damage in Eastern Australia

On 12 December, OM Australia settled on their first-ever permanent base after renting for over 20 years.

Teens in Europe are gathered this week to connect with Jesus and each other. A month ago, teenagers in Australia had this experience.

A former Afghan fighter discovers Jesus Christ in the Qur’an.