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Waldron Continues Competing At Hoosier Park

Harness racing driver Kiwon Waldron had a busy day at Harrah’s Hoosier Park in Indiana, competing in multiple races across both morning qualifiers and the evening program. Waldron’s day began with a second-place finish in the morning qualifiers, driving Aaron’s Ace to a 1:56.3 time in a pace class race. He later piloted Mr. Informed […]




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Waldron Resumes Competing At Hoosier Park

Harness racing driver Kiwon Waldron had a busy night at Harrah’s Hoosier Park in Indiana, participating in six races as the harness racing season rolls on. Waldron’s evening began with a trot race, where he piloted Rualone to a fifth-place finish, crossing the line in 1:59.3. He then switched gears to the pace class for […]




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Waldron Back In Harrah’s Hoosier Park Action

Harness racing driver Kiwon Waldron competed in two races at Harrah’s Hoosier Park in Indiana. In race 5, a pace event, Waldron guided Sweet Tell to a third-place finish with a time of 1:52.4. Later in the evening, Waldron drove Bodelicious in race 6, a trot race. The pair finished sixth with a time of […]




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Waldron Back In Action At Harrah’s Hoosier Park

Harness racer Kiwon Waldron competed in four races during a recent night of racing at Harrah’s Hoosier Park. Waldron’s night started with a fifth-place finish in the Trot Class aboard Mr Jessies Express in Race 1, clocking a time of 2:00.3. In Race 4, another Trot Class event, Waldron guided Bridge To Jesse’s to a […]




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Waldron Contests Four Races At Harrah’s Park

Kiwon Waldron participated in four races at Harrah’s Hoosier Park, consistently finishing mid-pack in a competitive evening of harness racing in Indiana. Waldron’s first race was in the pace class, where he drove Where’s Drogon to a sixth-place finish in a time of 1:53&3. In race 3, another pace class event, Waldron and Mary Jo […]




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Video: Police Identify Victim As Steve Parkes

The Bermuda Police Service identified the victim of Monday’s fatal shooting in the Mary Victoria Road area of Devonshire as 53-year-old Steve Parkes. The police said they do not believe Mr Parkes “was specifically targeted” and they “do believe that it was a situation of maybe the wrong place at the wrong time.” The police […]




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Bermudians Help Binfield Beat South Park

Bermudian goalkeeper Coleridge Fubler kept a clean sheet in Binfield’s 2-0 win at home to South Park in the Pitching In Isthmian League South Central Division One today [November 2]. Fellow Bermudians Reese Jones and Razhir Smith-Jones also started the match for the Berkshire outfit; meanwhile, Riley Robinson and Malachi Henry came on as second-half […]




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Heat Defeat The Storm At Bernard’s Park

In a Bermuda Netball Association Senior League make-up match the Phoenix Heat defeated the Storm 41 – 26. Danielle Raynor led the Phoenix Heat to victory with 32 goals, while Ebonie Cox added 9 goals. Eshe’ Grant was named the team’s best attacker, Kiante’ Lightbourne was named the best defender, and Nyisha Saunders was the […]




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Phoenix Defeat Docksiders At Bernard Park

The latest round of senior league netball action at the Bernard Park saw Phoenix — led by best attacker Danielle Raynor — defeat Docksiders by a score of 65 – 21. Raynor scored 54 goals, with MVP Ebonie Cox adding 11 goals, and Kiante’ Lightbourne named the best defender. Docksiders got 12 goals from best attacker […]




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BZS & Parks Department Continue Partnership

The Bermuda Zoological Society [BZS] and the Department of Parks spent time clearing invasives on Trunk Island recently in an effort to share knowledge and continue the partnership that has been evolving since the BZS Micro Forest Project began in 2021. “On June 26, members of the Parks team from Tulo Valley and Government House […]




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Keep Bermuda Beautiful Appoints Linda Parker

Keep Bermuda Beautiful [KBB] appointed Linda Parker as its part-time Education and Community Coordinator. A spokesperson said, “Keep Bermuda Beautiful [KBB] is delighted to announce the appointment of Linda Parker as its part-time Education and Community Engagement Coordinator. “Linda has a wealth of experience in Bermuda’s education sector as a teacher, Head of English, Deputy […]




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Kiwon Waldron Wins Race 10 At Hoosier Park

Kiwon Waldron was back in action competing at the Hoosier Park in Indiana. Waldron –  in the bike of Daniel’s Son –  in Hoosier Park Race 3 finished 4th clocking a time of 1:53.1. The Hoosier Park Race 10 saw Waldron and Spirt and Heart clock a winning time of 1:56.3. Related Stories Equestrian Patrick […]




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Dage Minors Wins Three Brooks Parkrun In UK

Dage Minors ran to victory in the 2024 Three Brooks Parkrun in the UK. Minors crossed the line in a time of 17:10 to defeat a field of 345 competitors. Related Stories Watson & Beaulieu Win Swan’s Charity 5K Ryan Outerbridge Wins 8k In Massachusetts Richardson Wins Long Beach 5 Mile Road Race Ryan Outerbridge […]




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TMQ To Host Juneteenth Jazz In The Park Event

The Tino Martinez Quartet [TMQ] will hold a Jazz in the Park event at Barr’s Bay Park on June 23 to honour Juneteenth. A spokesperson said, “Don’t worry if you missed the last Jazz in the Park, because you’re in luck. 2024 Best of Bermuda award winner TMQ is hosting their second jazz event in […]




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What Passes for Optimism at MacArthur Park

Beside the concrete pond, small children fish for nothing. This is all it costs to wish: a yard of yarn, a crooked stick. They cast their paper cups as if they might outlast hunger, as if a minnow might appear from muck and shoes and empty cans of beer. We watch them scoop up all …

The post What Passes for Optimism at MacArthur Park first appeared on Timothy Green.




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PAGE HAMILTON присоединился на сцене к LINKIN PARK

Лидер HELMET Page Hamilton присоединился на сцене к LINKIN PARK в рамках выступления восьмого ноября в Globe Life Field, Arlington, Texas и исполнил "All For Nothing". Группа также представила еще одну новую песню, "Casualty".
#Linkin_Park #LinkinPark #Rock #Rock #ElectroRock #Electro_Rock #PopRock #Pop_Rock #AlternativeRock #Alternative_Rock #AcousticRock #Acoustic_Rock #NuMetal #Nu_Metal


Видео: https://www.youtube.com/watch?v=wmU8w2ne-Kw
https://www.youtube.com/watch?v=ZJmIUfviqAI




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Free National Park Weekend

This weekend is another FREE pass to our National Parks.
Matthew Daly in Washington, D.C. Associated Press June 15, 2009

The U.S. National Park Service is looking to stimulate summer vacations at national parks, starting this weekend.

Interior Secretary Ken Salazar announced earlier this month that entrance fees at 147 national parks and monuments—including the Grand Canyon and Yosemite—will be waived on three weekends this summer. The weekends are June 20 and 21, July 18 and 19, and August 15 and 16.

"During these tough economic times, our national parks provide opportunities for affordable vacations for families," Salazar said at a news conference at Cuyahoga Valley National Park in Ohio.

"I encourage everyone to visit one of our nation's crown jewels this summer and especially to take advantage of the three free-admission weekends."

Most Americans live less than a day's drive from a national park, Salazar said. Last year national parks attracted more than 275 million visits, generating an estimated U.S. $10.6 billion for local economies and supporting more than 213,000 jobs, he said.

For the Park Service, the free weekends will mean a loss of an estimated half million dollars a day from entrance fees that range from $3 to $25. A total of 147 parks and monuments charge entrance fees. The country's other 244 parks are already free.

Kendra Barkoff, a spokesperson for Salazar, said the lost revenue should be more than offset by an increase in park tourism. Many tour operators, hotels, restaurants, gift shops, and other vendors near national parks will offer other discounts and special promotions on the free-weekend dates, she said.

The waiver applies only to entrance fees and does not affect charges for camping, reservations, tours, or concessions, Salazar said.

Senator Max Baucus, a Democrat representing Montana, applauded the free weekends. Baucus has co-sponsored a bill that would cap park entrance fees at current rates unless approved by Congress. The bill also would limit fees on national forests and other federally managed lands.

"There is nothing better than spending a weekend in Glacier or Yellowstone, and to be able to do it without straining the budget is even better," Baucus said in a statement. "Folks should be able to enjoy our outdoor heritage without going broke."

Kitty Benzar, president of the Western Slope No-Fee Coalition, a Colorado-based group that opposes fees on public lands, said Salazar's announcement was an admission that high fees are a deterrent to park visits.

"Twenty, 25 dollars does mean a lot to people," she said.

Copyright 2009 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten, or redistributed.




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Our National Parks, Part I

In honor of Ken Burn's documentary, "The National Parks, America's Best Idea," coming this fall, we're highlighting a few of our nation's topographic treasures.













This week: Yosemite National Park (Courtesy of National Geographic Traveler)
Location:Located in the Sierra Nevada range of California, the park is 195 miles east of San Francisco and 276 miles north of Los Angeles.

Vital Stats
Nearest airports: Fresno-Yosemite International, located 2.5 hours north on Highway 41. The San Francisco International airport is four hours east of Yosemite.

Established: Yosemite National Park was established in October 1890. Prior to the National Park Service (NPS), Yosemite was managed by the state and local congress. After the formation of the NPS, Yosemite Valley and the Mariposa Grove were reinstated in 1906 back to the federal government to be included in the management of the NPS.

Size: 761,266 acres
Park website: http://www.nps.gov/yose/
Snapshot: Recognized as a World Heritage site in 1984, Yosemite National Park covers over 745,000 acres of pristine wilderness filled with staggering cliffs, lakes, rivers, meadows, and a wealth of biological diversity. A haven to nature enthusiasts, artists, and families alike, the park is home to 800 miles of hiking trails, the tallest waterfall in the United States and the renowned giant sequoia groves.

Did You Know?
Towering more than 350 stories above Yosemite Valley, El Capitan is the largest exposed granite monolith in the world. By late August, Yosemite Falls are usually dry because the natural wonder relies solely on snowmelt. The peak flow is in late May and the falls return around October when the snow reappears. The park’s giant sequoia trees can live to be over 3,000 years old.

Scenic Drive
Approximately 48 miles in length, the Tioga Road is the most popular drive in Yosemite National Park. The road is the highest in the region, peaking at 9,945 feet at the Tioga Pass. The route winds through high peeks, meadows and creeks, allowing travelers to get a special view of the abundant wildlife in the park. Detour off of the main road to paths less traveled, including White Wolf, Siesta Lake, and the Red Fir Forest.
Read more...


You can explore, navigate, and create custom maps of 25 of America's National Parks with our digital mapping software, on sale for a limited time here: http://tinyurl.com/owy2x8




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Our National Parks, Part II

With the kickoff of Ken Burn's documentary series, "The National Parks, America's Best Idea," we continue our highlight of a few of our nation's topographic treasures.


This week: Grand Canyon National Park (Courtesy of nationalgeographic.com)
The road to the Grand Canyon from the south crosses a gently rising plateau that gives no hint at what is about to unfold. You wonder if you have made a wrong turn. All at once an immense gorge a mile deep and up to 18 miles wide opens up. The scale is so vast that even from the best vantage point only a fraction of the canyon's 277 miles can be seen.

Nearly five million people travel here each year; 90 percent first see the canyon from the South Rim with its dramatic views into the deep inner gorge of the Colorado River. So many feet have stepped cautiously to the edge of major overlooks that in places the rock has been polished smooth. But most of the park's 1,904 square miles are maintained as wilderness. You can avoid crowds by hiking the park's many trails or driving to the cool evergreen forests of the North Rim where people are fewer and viewing is more leisurely.

The Grand Canyon boasts some of the nation's cleanest air, with visibility averaging 90 to 110 miles. Increasingly, though, air pollution blurs vistas that once were sharp and rich hued. Hazy days have become more common, with visibility dropping as low as 40 miles. Haze from forest fires and pollen has always been present, but the recent increase is traced to sources outside the park, like copper smelters and urban areas in Arizona, southern California, and even Mexico.

read more ...

Coming Soon! Explore the wondrous Grand Canyon National Park with our new Trails Illustrated trail maps.







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Trump Posts a Photo of Himself Working on His Inaugural Address and it Gives Spark to a New Meme

Yesterday Trump tweeted a photo of himself hard at work on his inauguration speech and the internet has been having a field day with it. 

It started on twitter with people guessing at what The Donald might be drawing. Shortly thereafter it got a small photoshop battle. 

'What's Donald Drawing' definitely has the potential to catch on.

Get More Trump Memes that are simply tremendous, people tell me how amazing these memes are all the time.




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Ultimate Guide: Keep Your Dish Rack Sparkling Clean & Hygienic

Discover essential tips for maintaining a clean and hygienic dish rack in our latest article. Learn about the right cleaning methods, frequency, and products to keep your dish rack germ-free. This guide is a must-read for every homeowner seeking a healthier kitchen.

The post Ultimate Guide: Keep Your Dish Rack Sparkling Clean & Hygienic appeared first on Unclutterer.




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Trey Parker and Matt Stone Supposedly Bought Cars for a Whole Club of Strippers

By Keegan Kelly Published: November 11th, 2024




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‘South Park’s Timmy Is Inspiring New Bouts of Harassment Against Wheelchair Users

By Keegan Kelly Published: November 11th, 2024




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Casa Bonita Is Betting on Chlorine-Scented Candles to Make Trey Parker and Matt Stone’s Money Back

By JM McNab Published: November 12th, 2024




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Tennyson is Princeton’s new director of Transportation and Parking Services

Charles (Charlie) Tennyson has been appointed Princeton’s new director of Transportation and Parking Services following a national search to fill the position. He previously served for five years as deputy director of the department.





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Bike Check: Dangerholm's Scott Spark RC Visione



Another next-level build from Mr. Dangerholm.
( Photos: 31, Comments: 126 )




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Adapting national parks for wheelchair hiking

The trails through our public lands weren’t designed for wheelchairs, but new wheelchairs are designed for those trails. National Park Service accessibility specialist Quinn Brett wants parks to catch up with wheelchair technology, increasing access to American wilderness.




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Exploring the Enchantment of Zhangjiajie National Park: A Comprehensive Guide

Imagine stepping into a world where towering columns of rock stretch towards the sky, each shrouded in a veil of greenery and mist. This is not the realm of fantasy. This is Zhangjiajie National Forest Park in China, a place where nature’s artistry is on grand display. With landscapes so surreal, it’s no wonder that this park became a source of inspiration for the visually stunning world of Pandora in the movie “Avatar.” Zhangjiajie’s unique landscape is more than just a feast for the eyes; it’s a gateway to an adventure that promises to be as enchanting as it is exhilarating. From the ethereal beauty of its towering sandstone pillars to the deep, serene forests that blanket their bases, the park offers a tapestry of natural wonders waiting to be explored. This article is designed to be your comprehensive guide to navigating the spellbinding beauty of Zhangjiajie National Park. Whether you’re planning a visit or just dreaming of a journey to this otherworldly destination, we’ll take you through the heart of its magic, offering insights and tips to ensure your experience is as unforgettable as the landscape itself. Welcome to the gateway of a real-life Pandora, where the wonders of Zhangjiajie await. Is Zhangjiajie Worth Visiting? Address the central question directly Absolutely. Zhangjiajie Forest Park in China is a marvel of natural beauty that captivates all who visit. Its ethereal landscape is not just a visual treat but a soul-enriching experience. Highlight the park’s most distinctive features Quartzite Pillars: These towering, slender columns are the park’s signature feature. They rise dramatically from the ground, creating a surreal landscape that inspired the floating mountains in the movie “Avatar.” Diverse Flora and Fauna: Home to a rich biodiversity, the park shelters various species of plants and animals, some of which are rare and endemic to the region. Unique Karst Formations: The area showcases a fascinating range of karst phenomena, from deep ravines and gorges to natural bridges and caves, sculpted over millions of years. Briefly mention outdoor activities available Hiking: Trails wind through the park, offering routes for all abilities. They lead through dense forests, past serene streams, and up to breathtaking viewpoints. Cable Car Rides: For those who wish to take in the scenery without the hike, cable cars provide a stunning aerial view of the park. Sightseeing: Designated platforms and bridges offer spectacular vistas of the pillars, especially enchanting at sunrise or sunset. Planning Your Trip to Zhangjiajie Seasons Ideal Time to Visit: The best times to visit are spring (April to June) and fall (September to November). These seasons offer comfortable weather and the beauty of the park in full bloom or in the midst of autumnal colors. Peak vs. Shoulder Seasons: Summer (July and August) is peak tourist season, making the park more crowded. Winter sees fewer visitors, offering a quieter experience, albeit with cooler temperatures. Getting There Major Airports Nearby: The closest airport is Zhangjiajie Hehua International Airport, with connections to major Chinese cities. Transportation from Surrounding Cities: Buses and trains from cities like Changsha provide access to Zhangjiajie, making it reachable from various parts of China. Getting Around Shuttles: The park operates shuttle buses that connect different areas, making it easy to explore without excessive walking. Cable Cars and Elevators: To navigate the steep terrain, cable cars and the famous Bailong Elevator offer convenient and scenic rides. Walking Paths: For those who prefer to explore on foot, well-marked paths lead through the stunning scenery, allowing for an immersive experience. Must-See Sights in Zhangjiajie National Park Zhangjiajie National Park, a UNESCO World Heritage site, offers an array of natural wonders and cultural insights. Below are some of the must-see attractions within the park. Zhangjiajie National Forest The heart of Zhangjiajie’s magic lies in its iconic quartzite pillars, rising majestically from the earth. These formations are so unique that they inspired the floating “Hallelujah Mountains” in the blockbuster movie “Avatar.” The Avatar Hallelujah Mountain scenic area within the park pays homage to this connection, allowing visitors to step into a world that feels straight out of a science fiction fantasy. Tianzi Mountain Tianzi Mountain offers sweeping panoramic views that are unparalleled in their beauty. The area is renowned for its: Unique rock formations: The mountain’s landscape is dotted with towering pillars, each shaped by the forces of nature over millions of years. Points of interest: Notable spots include the Emperor Sightseeing Platform and Tianzi Pavilion, from where visitors can gaze out over an ocean of mist-enshrouded peaks. Huangshi Village (Yellow Lion Village) This traditional village offers a glimpse into the culture of the Tujia minority, showcasing their customs, architecture, and way of life. Huangshi Village is also celebrated for its: Cultural significance: The village is a living museum of Tujia traditions. Sunrise views: The location provides spectacular sunrise vistas, making it a favorite spot for early risers and photographers alike. Bailong Elevator The Bailong Elevator holds the record as the world’s highest outdoor elevator, whisking visitors up the side of a cliff face for an exhilarating ride. Highlights include: Breathtaking views: As you ascend, panoramic views of the surrounding landscape unfold, offering a unique perspective on the park’s natural beauty. Activities in Zhangjiajie National Park Zhangjiajie National Park is a playground for nature lovers, offering a plethora of activities that allow visitors to immerse themselves in its breathtaking landscapes. From challenging hikes to scenic cable car rides, there’s something for everyone in this majestic park. Hiking Hiking is undoubtedly one of the best ways to experience the natural beauty of Zhangjiajie National Park. The park boasts a range of trails that cater to various fitness levels, offering everyone the chance to explore its unique terrain. Golden Whip Stream: This trail is perfect for those who prefer a leisurely hike. It meanders alongside a crystal-clear stream, flanked by towering sandstone pillars. Bailong Elevator Path: For a more challenging adventure, the path leading from the base to the top of the Bailong Elevator allows hikers to experience the park’s verticality. This trail offers stunning views and a vigorous workout. Cable Car Rides For those who wish to take in the scenery without the physical exertion of hiking, cable car rides provide a spectacular alternative. Scenic Views: Cable cars glide over the park, offering panoramic views of the verdant landscapes and quartzite pillars that make Zhangjiajie so famous. Accessibility: This mode of transportation makes the park’s breathtaking beauty accessible to all, including those who may find hiking challenging. Photography Zhangjiajie National Park is a paradise for photographers, boasting an array of photogenic spots that capture the essence of its natural wonders. Photogenic Spots: Iconic locations like the Avatar Hallelujah Mountain and Tianzi Mountain offer unforgettable scenes for photography enthusiasts. Photography Tips: To capture the park’s landscapes at their best, aim for the golden hours of sunrise and sunset when the light casts a magical glow over the pillars and valleys. Essential Tips for Visitors to Zhangjiajie National Park Planning a trip to Zhangjiajie National Park can be an exciting adventure. To ensure a smooth and enjoyable experience, consider these essential tips covering accommodation, budget, weather, and other considerations. Accommodation Zhangjiajie offers a range of accommodation options to suit different preferences and budgets: Inside the Park: For a fully immersive experience, several hotels and guesthouses are located within the park itself. Staying here allows you to start exploring early in the morning before the crowds arrive. Outside the Park: The city of Zhangjiajie and nearby Wulingyuan offer more accommodation choices, from budget hostels to luxury hotels. These areas provide easy park access and additional dining options. Budget Understanding the costs associated with your visit can help you plan effectively: Park Entrance Fees: Entrance fees to the park vary by season, generally ranging from 225 to 245 RMB. Transportation: Costs will vary depending on your mode of travel within and to the park. Local buses and shuttle services are economical options. Food: Dining costs can vary widely, but budget-friendly options are available, especially in the city and at food stalls near the park. Accommodation: Prices range significantly based on location and comfort level. Budget accommodations start from around 100 RMB per night, while luxury options can exceed 1000 RMB. Weather and Clothing Zhangjiajie experiences distinct seasons, which can influence what you pack: Spring/Fall: These mild seasons require light layers that can be adjusted throughout the day. Summer: The weather can be hot and humid. Lightweight, breathable clothing and rain gear are advisable. Winter: Cold temperatures demand warm clothing and layers. Additional Considerations To enhance your trip, keep these tips in mind: Currency Exchange: It’s advisable to exchange some currency into RMB (Chinese Yuan) before arriving, especially for purchases in smaller shops and rural areas. Language Barriers: While Mandarin Chinese is the primary language, having a translation app or phrasebook can be invaluable. Some tourism workers speak basic English. Etiquette: Respect local customs and etiquette. For example, queue politely, speak respectfully, and ask permission before taking photos of locals. Conclusion Zhangjiajie National Park stands as a testament to nature’s artistry, offering a unique blend of breathtaking landscapes and rich cultural heritage. From the towering quartzite pillars that inspired the landscapes of “Avatar” to the serene beauty of its vast forests, the park provides an unparalleled adventure for nature lovers and adventurers alike. The diverse range of activities, from hiking the rugged trails to peaceful cable car rides, ensures that every visitor can find their own way to connect with this extraordinary place. We encourage you to plan your trip to Zhangjiajie and experience its magic firsthand. Witnessing the park’s majestic beauty and immersing yourself in its enchanting atmosphere is an adventure that promises to be unforgettable. FAQ How much time do I need to spend in Zhangjiajie? To truly appreciate the main highlights of Zhangjiajie National Park, we recommend spending a minimum of 2-3 days. This allows ample time to explore the iconic landscapes, take part in various activities, and soak in the natural beauty of the park. Is Zhangjiajie safe to visit? Yes, Zhangjiajie is safe for visitors. Like any travel destination, it’s wise to take basic precautions, stay on marked paths, and follow park guidelines to ensure a safe and enjoyable visit. What language is spoken in Zhangjiajie? The primary language spoken in Zhangjiajie is Mandarin Chinese. While some tourism resources and staff might be available in English, learning a few basic Mandarin phrases can enhance your experience. Are there any festivals or events held in Zhangjiajie? Zhangjiajie hosts various festivals and events throughout the year, celebrating the rich cultural traditions of the region. These can include traditional Tujia festivals, which are a fantastic way to experience local customs and entertainment. What are some local delicacies I should try? Zhangjiajie offers a variety of local delicacies that reflect its rich culinary tradition. Some must-try dishes include Tujia sour fish soup and flavorful stews, which are sure to provide a memorable dining experience.

The post Exploring the Enchantment of Zhangjiajie National Park: A Comprehensive Guide appeared first on Geeky Traveller.




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Paleontologist rates 14 dinosaur attacks in the "Jurassic Park" movies

Paleontologist Dave Hone rates the realism of the dinosaur attacks in the "Jurassic Park" and "Jurassic World" movies.




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50,000 bulbs to sparkle Rockefeller Christmas tree

NEW YORK (AP): The Rockefeller Center Christmas tree arrived in New York City on Saturday, signalling the start of the holiday season in the Big Apple. The 74-foot Norway spruce was driven into Manhattan's Center Plaza, where it was hoisted in...




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Bridge over troubled water - Bushy Park residents construct new walkway after floodwaters sweep away old one

After parking his taxi cab along the sidewalk, Leon Thompson exited his vehicle and held on tightly to the tiny hands of his four small passengers. They all walked towards a makeshift bridge, and Thompson lifted each child, making four trips,...




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Accurate MS-based Rab10 Phosphorylation Stoichiometry Determination as Readout for LRRK2 Activity in Parkinson's Disease [Research]

Pathogenic mutations in the Leucine-rich repeat kinase 2 (LRRK2) are the predominant genetic cause of Parkinson's disease (PD). They increase its activity, resulting in augmented Rab10-Thr73 phosphorylation and conversely, LRRK2 inhibition decreases pRab10 levels. Currently, there is no assay to quantify pRab10 levels for drug target engagement or patient stratification. To meet this challenge, we developed an high accuracy and sensitivity targeted mass spectrometry (MS)-based assay for determining Rab10-Thr73 phosphorylation stoichiometry in human samples. It uses synthetic stable isotope-labeled (SIL) analogues for both phosphorylated and nonphosphorylated tryptic peptides surrounding Rab10-Thr73 to directly derive the percentage of Rab10 phosphorylation from attomole amounts of the endogenous phosphopeptide. The SIL and the endogenous phosphopeptides are separately admitted into an Orbitrap analyzer with the appropriate injection times. We test the reproducibility of our assay by determining Rab10-Thr73 phosphorylation stoichiometry in neutrophils of LRRK2 mutation carriers before and after LRRK2 inhibition. Compared with healthy controls, the PD predisposing mutation carriers LRRK2 G2019S and VPS35 D620N display 1.9-fold and 3.7-fold increased pRab10 levels, respectively. Our generic MS-based assay further establishes the relevance of pRab10 as a prognostic PD marker and is a powerful tool for determining LRRK2 inhibitor efficacy and for stratifying PD patients for LRRK2 inhibitor treatment.




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Quantitative data independent acquisition glycoproteomics of sparkling wine [Research]

Sparkling wine is an alcoholic beverage enjoyed around the world. The sensory properties of sparkling wine depend on a complex interplay between the chemical and biochemical components in the final product. Glycoproteins have been linked to positive and negative qualities in sparkling wine, but the glycosylation profiles of sparkling wine have not been previously investigated in detail. We analysed the glyco/proteome of sparkling wines using protein- and glycopeptide-centric approaches. We developed an automated workflow that created ion libraries to analyse Sequential Window Acquisition of all THeoretical mass spectra (SWATH) Data Independent Acquisition (DIA) mass spectrometry data based on glycopeptides identified by Byonic. We applied our workflow to three pairs of experimental sparkling wines to assess the effects of aging on lees and of different yeast strains used in the Liqueur de Tirage for secondary fermentation. We found that aging a cuvée on lees for 24 months compared to 8 months led to a dramatic decrease in overall protein abundance and an enrichment in large glycans at specific sites in some proteins. Secondary fermentation of a Riesling wine with Saccharomyces cerevisiae yeast strain Siha4 produced more yeast proteins and glycoproteins than with S. cerevisiae yeast strain DV10. The abundance and glycosylation profiles of grape glycoproteins were also different between grape varieties. This work represents the first in-depth study into protein- and peptide-specific glycosylation in sparkling wines and describes a quantitative glycoproteomic SWATH/DIA workflow that is broadly applicable to other sample types.




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Marburg virus: First cases in Rwanda spark international alarm




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How to come together in a meaningful way | Priya Parker

Looking to infuse more connection into your time with friends and family? Facilitator Priya Parker urges us to approach gathering with intention and creativity. Diving into the evolving significance of coming together both in-person and virtually, she demystifies the role of a host, outlines mistakes we should avoid and shares the potential of thoughtfully designed get-togethers to bring lasting change to communities, workplaces and personal relationships. (This conversation was hosted by TED's Cloe Shasha Brooks. Visit ted.com/membership to support TED today and join more exclusive events like this one.)




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Teachers Without Internet Work in Parking Lots, Empty School Buildings During COVID-19

While most teachers have online access at home, internet service for many educators in rural areas is spotty, expensive, or nonexistent.




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Lawsuit Challenges Florida's Post-Parkland Plan to Arm Some School Employees

A Florida district's decision to put armed "school safety assistants" in its elementary schools puts the safety and well-being of its students at risk and oversteps existing state law, says a lawsuit, which could topple school security plans throughout the state.




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The Parkland Shooting's Role in the Close Florida Senate Race

The impact of the killings of students and staff at Marjory Stoneman Douglas High School could extend into the tight Florida contest that will help decide control of the U.S. Senate.




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Spark!Lab Ignites the Imagination

Interpretive exhibits coordinator Steven Madewells demonstrates a few experiments at Spark!Lab, an interactive exhibit at the National Museum of American History. Read more at: https://www.smithsonianmag.com/smithsonian-institution/theres-nothing-like-a-sparklab-to-ignite-the-imagination-1-180949007/




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5 Surprising Facts About Rosa Parks

Largely recognized for her leading role in the bus boycott of 1955, Rosa Parks was an enduring activist for the Civil Rights Movement. Throughout her numerous decades of service, Parks left behind a legacy of hope, inspiration, and leadership for millions around the world. --- For more videos from Smithsonian Magazine: https://www.smithsonianmag.com/videos/ Digital Editorial Director: Brian Wolly Supervising Producer & Scriptwriter: Michelle Mehrtens Video Editor: Sierra Theobald




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Virginia State Parks Install Viewfinders for People With Colorblindness, Just in Time for Leaf-Peeping Season

The viewfinders are outfitted with special lenses that help people with red-green colorblindness distinguish between hues




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The Highest Peak in Great Smoky Mountains National Park Will Now Be Called by Its Cherokee Name

In 1858, the mountain was named for a Confederate general. Now, it will once again be known as "Kuwohi"




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This Park Recreates Vincent van Gogh's 'The Starry Night' With a Dazzling Display of Plants, Trees and Winding Pathways

At a new park in Bosnia and Herzegovina, two dozen gardeners have spent years replicating the Dutch artist's masterpiece using the land as their canvas




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This New York City Park Was Built on Top of a Cemetery

In the late 19th century, city officials turned the final resting place for 10,000 souls into what's now Greenwich Village’s James J. Walker Park




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Who's sparking joy in your community? Let us know about the people making things better

CBC Newfoundland and Labrador would love to hear your suggestions of folks and organizations who are there when you need them — and all the other times, too.



  • News/Canada/Nfld. & Labrador

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Body found in Montreal nature park was that of kidnapped cryptocurrency influencer, police say

Quebec provincial police have confirmed that the body found in a Montreal nature park on Oct. 30 was that of kidnapping victim and cryptocurrency influencer Kevin Mirshahi.



  • News/Canada/Montreal