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Spatially isolated reactions in a complex array: using magnetic beads to purify and quantify nucleic acids with digital and quantitative real-time PCR in thousands of parallel microwells

Lab Chip, 2020, Advance Article
DOI: 10.1039/D0LC00069H, Paper
W. Hampton Henley, Nathan A. Siegfried, J. Michael Ramsey
Encoded beads carrying primer pairs for nucleic acid targets are used for sample preparation and multiplexed-in-space digital PCR quantification.
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Acoustic separation of living and dead cells using high density medium

Lab Chip, 2020, Advance Article
DOI: 10.1039/D0LC00175A, Paper
Open Access
Karl Olofsson, Björn Hammarström, Martin Wiklund
A novel, simple and robust route for binary separation of viable and dead cells using a density modified medium which takes advantage of the compromised cell membrane of dead cells.
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PRESCIENT: platform for the rapid evaluation of antibody success using integrated microfluidics enabled technology

Lab Chip, 2020, 20,1628-1638
DOI: 10.1039/C9LC01165J, Paper
Jose A. Wippold, Han Wang, Joseph Tingling, Julian L. Leibowitz, Paul de Figueiredo, Arum Han
Identifying antibodies (Abs) that neutralize infectious agents is the first step for developing therapeutics, vaccines, and diagnostic tools for these infectious agents.
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Auto-affitech: an automated ligand binding affinity evaluation platform using digital microfluidics with a bidirectional magnetic separation method

Lab Chip, 2020, 20,1577-1585
DOI: 10.1039/D0LC00024H, Paper
Jingjing Guo, Li Lin, Kaifeng Zhao, Yanling Song, Mengjiao Huang, Zhi Zhu, Leiji Zhou, Chaoyong Yang
An automated ligand binding affinity evaluation platform using digital microfluidics with a bidirectional magnetic separation method that reduces the bead loss.
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Monitoring the two-dimensional concentration profile of toluene vapors by using polymer-stabilized nematic liquid crystals in microchannels

Lab Chip, 2020, 20,1687-1693
DOI: 10.1039/C9LC01021A, Paper
Zongdai Liu, Dan Luo, Kun-Lin Yang
The concentration of toluene vapor is obtained based on polymer-stabilized liquid crystals by viewing a colourful gamut under a polarized optical microscope.
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In-flow measurement of cell–cell adhesion using oscillatory inertial microfluidics

Lab Chip, 2020, 20,1612-1620
DOI: 10.1039/D0LC00089B, Paper
Baris R. Mutlu, Taronish Dubash, Claudius Dietsche, Avanish Mishra, Arzu Ozbey, Kevin Keim, Jon F. Edd, Daniel A. Haber, Shyamala Maheswaran, Mehmet Toner
Cell–cell adhesion strength of freely suspended cell clusters can be measured using an oscillatory inertial microfluidic system.
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3-Step flow focusing enables multidirectional imaging of bioparticles for imaging flow cytometry

Lab Chip, 2020, 20,1676-1686
DOI: 10.1039/D0LC00244E, Paper
Open Access
  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Andreas Kleiber, Anuradha Ramoji, Günter Mayer, Ute Neugebauer, Jürgen Popp, Thomas Henkel
The control of the focus plane allows multi-directional imaging flow cytometry.
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Particle/cell separation using sheath-free deterministic lateral displacement arrays with inertially focused single straight input

Lab Chip, 2020, Advance Article
DOI: 10.1039/D0LC00354A, Paper
Naotomo Tottori, Takasi Nisisako
We propose sheath-free microfluidic deterministic lateral displacement devices with inertially focused single straight input.
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[ASAP] Comparing Cryo-EM Reconstructions and Validating Atomic Model Fit Using Difference Maps

Journal of Chemical Information and Modeling
DOI: 10.1021/acs.jcim.9b01103




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[ASAP] Flexible Fitting of Small Molecules into Electron Microscopy Maps Using Molecular Dynamics Simulations with Neural Network Potentials

Journal of Chemical Information and Modeling
DOI: 10.1021/acs.jcim.9b01167




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[ASAP] Brick-CFCMC: Open Source Software for Monte Carlo Simulations of Phase and Reaction Equilibria Using the Continuous Fractional Component Method

Journal of Chemical Information and Modeling
DOI: 10.1021/acs.jcim.0c00334




using

[ASAP] Multisolvent Models for Solvation Free Energy Predictions Using 3D-RISM Hydration Thermodynamic Descriptors

Journal of Chemical Information and Modeling
DOI: 10.1021/acs.jcim.0c00065




using

[ASAP] Accelerated Protein Folding Using Greedy-Proximal A*

Journal of Chemical Information and Modeling
DOI: 10.1021/acs.jcim.9b01194




using

[ASAP] Unraveling the Electric Field-Induced Second Harmonic Generation Responses of Stilbazolium Ion Pairs Complexes in Solution Using a Multiscale Simulation Method

Journal of Chemical Information and Modeling
DOI: 10.1021/acs.jcim.9b01161




using

[ASAP] Ranking of Ligand Binding Kinetics Using a Weighted Ensemble Approach and Comparison with a Multiscale Milestoning Approach

Journal of Chemical Information and Modeling
DOI: 10.1021/acs.jcim.9b00968




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Here's how you can boost your immunity using Ayurveda in wake of coronavirus crisis

The Ministry of AYUSH has recommended the following self-care guidelines for preventive health measures and boosting immunity with special reference to respiratory health.




using

Housing Estates in the Baltic Countries [electronic resource] : The Legacy of Central Planning in Estonia, Latvia and Lithuania / edited by Daniel Baldwin Hess, Tiit Tammaru




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Using visual data in qualitative research [electronic resource] / Marcus Banks

Banks, Marcus




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Studies of the labile lead pool using a rhodamine-based fluorescent probe

Metallomics, 2020, Advance Article
DOI: 10.1039/D0MT00056F, Communication
Jianping Zhu, Jia Hao Yeo, Amy A. Bowyer, Nicholas Proschogo, Elizabeth J. New
A rhodamine-based fluorescent lead probe reports on the labile lead pool within cells.
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[ASAP] In Vitro Identification of the Hamiltonian Cycle Using a Circular Structure Assisted DNA Computer

ACS Combinatorial Science
DOI: 10.1021/acscombsci.9b00150




using

[ASAP] Experimental and Theoretical Investigation on Phase Formation and Mechanical Properties in Cr–Co–Ni Alloys Processed Using a Novel Thin-Film Quenching Technique

ACS Combinatorial Science
DOI: 10.1021/acscombsci.9b00170




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[ASAP] Kinetic Analysis of Electrochemical Lactonization of Ketones Using Water as the Oxygen Atom Source

ACS Catalysis
DOI: 10.1021/acscatal.0c00931




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[ASAP] Photocatalytic Deoxygenation of Sulfoxides Using Visible Light: Mechanistic Investigations and Synthetic Applications

ACS Catalysis
DOI: 10.1021/acscatal.0c00690




using

Tracking ancient Rome’s rise using Greenland’s ice, and fighting fungicide resistance

Two thousand years ago, ancient Romans were pumping lead into the air as they smelted ores to make the silvery coin of the realm. Online News Editor David Grimm talks to Sarah Crespi about how the pollution of ice in Greenland from this process provides a detailed 1900-year record of Roman history. This week is also resistance week at Science—where researchers explore the global challenges of antibiotic resistance, pesticide resistance, herbicide resistance, and fungicide resistance. Sarah talks with Sarah Gurr of the University of Exeter in the United Kingdom about her group’s work on the spread of antifungal resistance and what it means for crops and in the clinic. And in a bonus books segment, staff writer Jennifer Couzin-Frankel talks about medicine and fraud in her review of Bad Blood: Secrets and Lies in a Silicon Valley Startup by John Carreyrou. This week’s episode was edited by Podigy. Listen to previous podcasts. [Image: Wheat rust/Oregon State University; Music: Jeffrey Cook]




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Sketching suspects with DNA, and using light to find Zika-infected mosquitoes

DNA fingerprinting has been used to link people to crimes for decades, by matching DNA from a crime scene to DNA extracted from a suspect. Now, investigators are using other parts of the genome—such as markers for hair and eye color—to help rule people in and out as suspects. Staff Writer Gretchen Vogel talks with Sarah Crespi about whether science supports this approach and how different countries are dealing with this new type of evidence. Sarah also talks with Jill Fernandes of the University of Queensland in Brisbane, Australia, about her Science Advances paper on a light-based technique for detecting Zika in mosquitoes. Instead of grinding up the bug and extracting Zika DNA, her group shines near-infrared light through the body. Mosquitoes carrying Zika transmit this light differently from uninfected ones. If it’s successful in larger trials, this technique could make large-scale surveillance of infected mosquitoes quicker and less expensive. In our monthly books segment, Jen Golbeck talks with author Sarah-Jayne Blakemore about her new work: Inventing Ourselves: The Secret Life of the Teenage Brain. You can check out more book reviews and share your thoughts on the Books et al. blog. This week’s episode was edited by Podigy. Listen to previous podcasts. [Image: National Institute of Allergy and Infectious Diseases, National Institutes of Health; Music: Jeffrey Cook]




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Spotting slavery from space, and using iPads for communication disorders

In our first segment from the annual meeting of AAAS (Science’s publisher) in Washington, D.C., host Sarah Crespi talks with Cathy Binger of University of New Mexico in Albuquerque about her session on the role of modern technology, such as iPads and apps, in helping people with communication disorders. It turns out that there’s no killer app, but some devices do help normalize assistive technology for kids. Also this week, freelance journalist Sarah Scoles joins Sarah Crespi to talk about bringing together satellite imaging, machine learning, and nonprofits to put a stop to modern-day slavery. In our monthly books segment, books editor Valerie Thompson talks with Judy Grisel about her book Never Enough: The Neuroscience and Experience of Addiction, including discussions of Gisel’s personal experience with addiction and how it has informed her research as a neuroscientist. This week’s episode was edited by Podigy. Download the transcript (PDF) Listen to previous podcasts. About the Science Podcast [Image: ILO in Asia and the Pacific/Flickr; Music: Jeffrey Cook]




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The point of pointing, and using seabirds to track ocean health

You can learn a lot about ocean health from seabirds. For example, breeding failures among certain birds have been linked to the later collapse of some fisheries. Enriqueta Velarde of the Institute of Marine Sciences and Fisheries at the University of Veracruz in Xalapa, Mexico, joins host Sarah Crespi to talk about what these long-lived fliers can tell us about the ocean and its inhabitants. Also this week, Sarah and Cathal O’Madagain of the Ecole Normale Supérieure in Paris discuss pointing—a universal human gesture common to almost all children before age 1. They discuss why pointing matters, and how this simple gesture may underlie humans’ amazing ability to collaborate and coordinate. This week’s episode was edited by Podigy. Ads on the show: Kiwico.com Listen to previous podcasts. About the Science Podcast [Image: C. O’Madagain et al., Science Advances 2019; Music: Jeffrey Cook]




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Double dipping in an NIH loan repayment program, and using undersea cables as seismic sensors

The National Institutes of Health’s largest loan repayment program was conceived to help scientists pay off school debts without relying on industry funding. But a close examination of the program by investigative correspondent Charles Piller has revealed that many participants are taking money from the government to repay their loans, while at the same time taking payments from pharmaceutical companies. Piller joins Host Sarah Crespi to talk about the steps he took to uncover this double dipping and why ethicists say this a conflict of interest.   Sarah also talks with Nate Lindsey, a Ph.D. candidate at the University of California, Berkeley, about turning a 50-kilometer undersea fiber optic cable designed to move data into a sensor for activity in the ocean and the land underneath. During a 4-day test in Monterey Bay, California, the cable detected earthquakes, faults, waves, and even ocean-going storms. For this month’s books segment, Kiki Sandford talks with Dan Hooper about his book At the Edge of Time: Exploring the Mysteries of Our Universe’s First Seconds.   You can find more books segments on the Books et al. blog.   This week’s episode was edited by Podigy.   Ads on this week’s show: McDonalds; Salk’s Where Cures Begin podcast   Download the transcript (PDF) Listen to previous podcasts.   About the Science Podcast




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Product :: Apple Pro Training Series: OS X Server Essentials 10.10: Using and Supporting OS X Server on Yosemite




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A deep learning approach to identify association of disease–gene using information of disease symptoms and protein sequences

Anal. Methods, 2020, 12,2016-2026
DOI: 10.1039/C9AY02333J, Paper
Xingyu Chen, Qixing Huang, Yang Wang, Jinlong Li, Haiyan Liu, Yun Xie, Zong Dai, Xiaoyong Zou, Zhanchao Li
Prediction of disease–gene association based on a deep convolutional neural network.
The content of this RSS Feed (c) The Royal Society of Chemistry




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Fast determination of five chiral antipsychotic drugs using dispersive liquid–liquid microextraction combined with capillary electrophoresis

Anal. Methods, 2020, 12,2002-2008
DOI: 10.1039/C9AY02776A, Paper
Ming-Mu Hsieh, Tai-Chia Chiu, Szu-Hua Chen
This study developed a new method for the extraction, clean up, chiral separation, and determination of five pairs of phenothiazine drugs using ultrasound-assisted dispersive liquid–liquid microextraction combined with capillary electrophoresis.
The content of this RSS Feed (c) The Royal Society of Chemistry




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Bio-assay of non-amidated progastrin-derived peptide (G17-Gly) using Tailor-made recombinant antibody fragment and phages display method: A biomedical analysis

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/D0AY00627K, Paper
Deniz Sadighbayan, Mohammad Reza Tohid-kia, Tayebeh Mehdipour, Mohammad Hasanzadeh, Ahmad Yari Khosroushahi
In this research, four novel and sensitive immunosensor for electrochemical determination of G17-Gly were designed based on signal amplification and tailor-made recombinant antibody technology. Anti-G17-Glyantibody fragments (i.e. scFv and VL...
The content of this RSS Feed (c) The Royal Society of Chemistry




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Methodology for elemental analysis of mineral fertilizer, some of its raw materials and limestone using microwave-induced plasma optical emission spectrometry (MIP OES)

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/D0AY00459F, Paper
Dirce Pozebon, Alexandre Müller, Anderson Schwingel Ribeiro
Elemental analysis of complex matrices such as superphosphate-fertilizer and agricultural inputs by means of microwave induced plasma optical emission has been evaluated in the present study. A commercial single superphosphate-fertilizer...
The content of this RSS Feed (c) The Royal Society of Chemistry




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Determination of total and bioavailable of As and Sb in children’s paints using MSFIA system coupled to HG-AFS

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/C9AY02779C, Paper
Dayara Virginia Lino Ávila, Sidnei OLIVEIRA Souza, Victor Cerdà, Rennan Geovanny Oliveira Araujo
In this work, the application of Doehlert design for the optimisation aiming the determination of As and Sb in gouache and tempera children's paints, using multi-syringe flow injection analysis (MSFIA)...
The content of this RSS Feed (c) The Royal Society of Chemistry




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Detection of Nutrition and Toxic Elements in Pakistani Pepper Powders Using Laser Induced Breakdown Spectroscopy

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/D0AY00630K, Paper
Imran Rehan, Kamran Rehan, Muhammad Zubair Khan, Sabiha Sultana, Riaz Khan, Hamdullah Khan
In the current paper, we applied laser induced breakdown spectroscopy (LIBS) to determine the elemental distribution of nutritional and trace heavy metals in pepper powders available in Pakistan using standard...
The content of this RSS Feed (c) The Royal Society of Chemistry




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Detection of prostate-specific antigen in semen using DNA aptamers: an application of nucleic acid aptamers in forensic body fluid identification

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/D0AY00371A, Paper
Tetsuya Satoh, Seiya Kouroki, Yusuke Kitamura, Toshihiro Ihara, Kazutoshi Matsumura, Susumu Iwase
In forensics, body fluid identification plays an important role because it aids in reconstructing the crime scene. Therefore, it is essential to develop simple and reliable techniques for body fluid...
The content of this RSS Feed (c) The Royal Society of Chemistry




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Direct measurement of Cu and Pb isotopic ratios without column chemistry for bronzes using MC-ICP-MS

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/D0AY00561D, Paper
Zhian Bao, Chunlei Zong, Peng Liang, Yan Zhang, Kaiyun Chen, Honglin Yuan
This study presents a practical method for high-precision Cu and Pb isotope determination without column chemistry for bronzes using MC-ICP-MS. The standard-sample bracketing method combined with Ga internal normalization (SSBIN)...
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Rapid detection of quality of Japanese fermented soy sauce using near-infrared spectroscopy

Anal. Methods, 2020, Advance Article
DOI: 10.1039/D0AY00521E, Paper
Shuo Wang, Takehiro Tamura, Nobuyuki Kyouno, Xiaofang Liu, Han Zhang, Yoshinobu Akiyama, Jie Yu Chen
The application of NIR spectroscopy has great potential as an alternative quality control method, which provides a robust model for routinely estimating the final quality of soy sauce production rapidly and economically.
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Fast magnetic solid-phase extraction using Fe3O4-NH2@MOF material for monohydroxy polycyclic aromatic hydrocarbons in urine of coke-oven workers

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/D0AY00449A, Paper
Yahui Wang, Meng Yan, Qianqian Ji, Manman Wang, Qian Wang, Xue-Sheng Wang, Yulan Hao
In this work, magnetic material (Fe3O4-NH2@MIL-101) was successfully prepared, and the material was used as a sorbent for the magnetic solid-phase extraction (MSPE) of trace level monohydroxy polycyclic aromatic hydrocarbons...
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Improving the determination of celecoxib in body fluids and pharmaceuticals using a new selective and thermosensitive molecularly imprinted poly(vinylidene fluoride) membrane

Anal. Methods, 2020, 12,2185-2195
DOI: 10.1039/D0AY00237B, Paper
Negin Yazdanian, Behrouz Akbari-Adergani, Maryam Kazemipour, Homayon Ahmad Panahi, Mehran Javanbakht
Molecularly imprinted membranes that demonstrated high selectivity for celecoxib were synthesized using N-vinylcaprolactam and 2-hydroxyethyl methacrylate.
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Multi-residue determination of micropollutants in Nigerian fish from Lagos lagoon using ultrasound assisted extraction, solid phase extraction and ultra-high-performance liquid chromatography tandem mass spectrometry

Anal. Methods, 2020, 12,2114-2122
DOI: 10.1039/D0AY00411A, Paper
Idera Fabunmi, Natalie Sims, Kathryn Proctor, Aderonke Oyeyiola, Temilola Oluseyi, Kehinde Olayinka, Barbara Kasprzyk-Hordern
This reports for the first time a simple and robust approach in determining pharmaceuticals in different fish species in Nigeria.
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Determination of polychlorinated biphenyls and organochlorine pesticides in Chinese mitten crabs (Eriocheir sinensis) using modified QuEChERS followed by GC-MS

Anal. Methods, 2020, Advance Article
DOI: 10.1039/D0AY00519C, Paper
Ting Liu, Jianguang Zhou, Li He, Jinhua Gan
A new QuEChERS method followed by GC-MS was developed for the simultaneous analysis of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in Chinese mitten crabs.
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Rh(III) hydroxocomplexes speciation using HPLC-ESI-MS

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/D0AY00363H, Paper
Victoria V Volchek, Semen Nikolaevich Berdyugin, Olga Vasil`evna Shuvaeva, Dmitriy Sheven, Danila Vasilchenko, Sergey Korenev
A mixture of rhodium(III) hydroxocomplexes formed during the polycondensation process in alkaline media has been fully characterized by the hyphenated high performance liquid chromatography with electrospray ionization mass spectrometry (HPLC-ESI-MS)....
The content of this RSS Feed (c) The Royal Society of Chemistry




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Colorimetric speciation analysis of chromium using 2-thiobarbituric acid capped silver nanoparticles

Anal. Methods, 2020, Advance Article
DOI: 10.1039/D0AY00160K, Paper
Kamal Mousapour, Salahaddin Hajizadeh, Khalil Farhadi
Colorimetric determination of Cr(III) and Cr(VI) based on 2-thiobarbituric acid capped silver nanoparticles.
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Confirmation of brand identification in infant formulas by using near-infrared spectroscopy fingerprints

Anal. Methods, 2020, Advance Article
DOI: 10.1039/D0AY00375A, Paper
Shuyi Chang, Chengcheng Yin, Sha Liang, Mei Lu, Ping Wang, Zhicheng Li
The NIR spectra of 12 batches of each stage of Brand A infant formulas.
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Solid-phase microextraction using a β-ketoenamine-linked covalent organic framework coating for efficient enrichment of synthetic musks in water samples

Anal. Methods, 2020, Advance Article
DOI: 10.1039/C9AY02755F, Paper
Lian Wen, Peng Wu, Lei-Lei Wang, Li-Zong Chen, Ming-Lin Wang, Xia Wang, Jin-Ming Lin, Ru-Song Zhao
This study indicated the promising applicability of the TpPa-1 as a solid-phase microextraction fiber coating for reliably detecting synthetic musks at trace levels from environmental samples.
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A high-throughput plasmonic tongue using an aggregation assay and nonspecific interactions: classification of taste profiles in maple syrup

Anal. Methods, 2020, Advance Article
DOI: 10.1039/C9AY01942A, Paper
Simon Forest, Trevor Théorêt, Julien Coutu, Jean-Francois Masson
A simple colorimetric test detects off-flavour profiles of maple syrups in minutes, which are detectable by the naked eye.
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Detection of organophosphorus compounds using a surface acoustic wave array sensor based on supramolecular self-assembling imprinted films

Anal. Methods, 2020, 12,2206-2214
DOI: 10.1039/D0AY00211A, Paper
Yong Pan, Tengxiao Guo, Genwei Zhang, Junchao Yang, Liu Yang, Bingqing Cao
In this study, diisopropyl methylphosphonate (DIMP), tributyl phosphate (TBP), and dimethyl methylphosphonate (DMMP) were selected as organophosphorus chemical warfare agent (CWA) simulants.
The content of this RSS Feed (c) The Royal Society of Chemistry




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Adsorptive removal of Hg2+ from environmental water samples using thioglycerol-intercalated magnetic layered double hydroxides

Anal. Methods, 2020, 12,2279-2286
DOI: 10.1039/D0AY00448K, Paper
Ali Esrafili, Mahnaz Ghambarian, Mohammad Tajik, Mahroo Baharfar
Herein, the removal of Hg2+ from environmental water samples was carried out using a novel nanoadsorbent based on magnetite nanoparticles coated by a thioglycerol-intercalated layered double hydroxide.
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A simple paper-based approach for arsenic determination in water using hydride generation coupled with mercaptosuccinic-acid capped CdTe quantum dots

Anal. Methods, 2020, Accepted Manuscript
DOI: 10.1039/D0AY00273A, Paper
Oraphan Thepmanee, Kanlaya Prapainop Katewongsa, Obnithi Nooppha, Nuanlaor Ratanawimarnwong, Weena Siangproh, Orawon Chailapakul, Kriangsak Songsrirote
This research aims to develop a simple paper-based device for arsenic detection in water samples where a hydride generation technique coupled with mercaptosuccinic acid-capped CdTe quantum dots (MSA-CdTe QDs) as...
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