[London], [1827?]

[Spain?], An. 1817.

London (No. 18 & 19 Southampton Place, Euston Square, New Road) : Published ... by the proprietors [E. & W. Finden], May 1, 1839.

[London?] : Cassell & Company Limited, [between 1800 and 1899]

[London] : [Wellcome Trust], [2009]

[London] : Waterlow Bros. & Layton, Limited, [1893]

London : Printed and sold by Thos. and John Bowles, printsellers, [1728]

[London] : [Thomas. Bowles] : [John Bowles], [1728]

A union lobbyist who worked just one day as a substitute teacher is entitled to a pension worth potentially tens of thousands of dollars annually, the Illinois supreme court has ruled.

'The Comeback on Katella' was complete when Corey Perry scored the winning goal in double overtime to give the Ducks a 4-3 victory and 3-2 series lead.

It's been two years since Kuzy sent the Caps to the Eastern Conference Final with a legendary goal in overtime.

The win was the first of four straight for the Flyers, culminating in a historic comeback over the Bruins.

[Place of publication not identified] : [publisher not identified], [1989?]

This paper develops deterministic upper and lower bounds on the influence measure in a network, more precisely, the expected number of nodes that a seed set can influence in the independent cascade model. In particular, our bounds exploit r-nonbacktracking walks and Fortuin-Kasteleyn-Ginibre (FKG) type inequalities, and are computed by message passing algorithms. Further, we provide parameterized versions of the bounds that control the trade-off between efficiency and accuracy. Finally, the tightness of the bounds is illustrated on various network models.

Katarzyna Jańczak-Borkowska.

Source: Brazilian Journal of Probability and Statistics, Volume 33, Number 3, 480--497.

Abstract:
We prove the existence and uniqueness of the solution of backward stochastic variational inequalities with respect to fractional Brownian motion and with non-Lipschitz coefficient. We assume that $H>1/2$.

Ammunition Depot comments on Judge Roger T. Benitez ruling that Californians may again purchase ammo without a background check and order ammo online.

(PRWeb April 24, 2020)

Read the full story at https://www.prweb.com/releases/gun_rights_california_gun_owners_ammo_dealers_fire_back_against_proposition_63/prweb17075447.htm

Gambling (Family)

Saint Paul schools are in the market for a vendor to provide background checks, while the Education Technology Joint Powers Authority is seeking media repositories. A Texas district wants quotes on technology for new campuses.

The post Calif. Ed-Tech Consortium Seeks Media Repository Solutions; Saint Paul District Needs Background Check Services appeared first on Market Brief.

The reaction on social media has been fierce.

Spanish translation of the BIS press release about the BIS Quarterly Review, December 2018

After days and days of heavy rain, Hernan Espinoza looked out over his fields. They were waterlogged, some completely destroyed. He was like many others in his small community of Tambogrande on Peru’s northern coast: grappling with devastation, their farms and fields severely damaged by flooding, storms and landslides, caused by the El Niño Costero (coastal) phenomenon that pounded the [...]

Youth around the world are increasingly turning away from agriculture. Traditionally requiring tough manual labour and offering low wages, agriculture does not often appeal to new generations who generally prefer to try their luck finding jobs in cities.  

‘What on earth were they doing there?’ one researcher asks

A recent excavation located the first physical evidence of the capital of the Maya kingdom of Sak Tz'i', founded in 750 B.C.

Scientists discover neurotoxin-producing bacteria living on the skin of rough-skinned newts

Beaches in Florida and Thailand have tentatively reported increases in nests, due to decreased human presence. But the trend won’t necessarily persist

Rumored to withstand the weight of a full grown man, their spines have now been studied in unprecedented detail

Root vegetables like rutabagas and Jerusalem artichokes were ration staples during the Nazi occupation of Paris

A Guanaco (Lama guanicoe) surveys its surroundings from a vantage point at sunset, with the imposing Torres del Paine (Chile) in the background. Scouts like this individual keep an eye out for predators.

Alcohol industry groups were successful in getting the Yukon government to pull labels warning of the connection between alcohol and cancer from liquor store shelves, but the strategy may have ultimately backfired, researchers suggest.

A 19-year-old man was flown back to Ontario after he pleaded guilty to violating New Brunswick’s emergency measures order prohibiting visitors from entering the province.

The premier’s office confirmed the news in an email statement, blaming visibility issues under "very specific lighting conditions."

Philip Pynn is being released from federal prison for a second time, after breaching conditions landed him back behind bars last year — but it's not clear where he can live.

Staff halted $100 service over pandemic concerns, forcing homeowners to private plumbers much more, and Danny Breen is urging them to contact the city.

"It is unfortunate that it has come to pass that we have to collectively and overtly publicly declare that we are unified," says Alberta Medical Association president Christine Molnar.

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.

This protocol describes the preparation of the shuttle vector before its introduction into bacterial artificial chromosome (BAC) host cells for BAC two-step engineering. The homology arm sequences, prepared previously, are introduced by ligation into the digested shuttle vector DNA to provide sites for recombination within the BAC clone. Crude lysates of individual bacterial transformants serve as templates in polymerase chain reaction (PCR) analysis to confirm the presence of the homology arms in the recombinant shuttle vector.

The 700-bp A homology arm (A-box) and the 700-bp B homology arm (B-box) are amplified by polymerase chain reaction (PCR) using purified bacterial artificial chromosome (BAC) DNA as template for two-step BAC engineering. The resulting A-box PCR product contains an AscI site at its 5' end (the 5' primer incorporates an AscI site, and the 3' primer does not incorporate any restriction sites). The B-box PCR product contains an XmaI site at its 3' end (the 5' primer does not incorporate any restriction sites, and the 3' primer incorporates an XmaI site). The amplification products are then digested with the appropriate restriction endonucleases to render them suitable for cloning into the shuttle vector.

In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

Prices on P.E.I. for gas, heating oil and diesel all took a big jump upwards Friday.

Former Michigan Gov. Rick Snyder says he won’t continue with a fellowship at Harvard University following backlash over his role in Flint’s water crisis.