Sir Stirling Moss recalls the two serious Formula One crashes that nearly cost him his life.

Allan Little examines how events on VE Day were reported by the BBC, and how Britain changed.

BBC Sport looks at the path La Liga is following to allow top-flight football to resume safely in Spain.

A chronology of key events

Key facts, figures and dates

US space agency satellites follow the melting trends in Antarctica and Greenland over 16 years.

The actor is well known for his daring stunts, but this takes things to another level.

Is Sir Richard Branson's space tourism venture, Virgin Galactic, looking for a European base?

The "Diary of a Wimpy Kid" author, a "Peanuts" studio creator and others explain the ways they're staying creative during a pandemic.

Steenz and Bianca Xunise have entered a field that has long overlooked the voices of African American women.

Amazon said it would hire 100 000 warehouse and delivery workers in the United States to deal with a surge in online orders, as many consumers have turned to the web to meet their needs during the coronavirus outbreak.

velocityconf: RT @allspaw: Operations and System Safety - always a student http://t.co/1VYBEZYrK8 #devops #velocityconf

An alleged robber, who was wounded while his two suspected accomplices were killed in a shootout with police, has been arrested.

Madrid, Spain

Andalusia, Southern Spain

Barcelona, Spain

Valencia, Spain

The Amateur Traveler talks to Ben Dixon about biking the route of the Camino di Santiago in northern Spain. The Camino di Santiago is a an ancient pilgrim trail that runs across northern Spain from the border with France to Santiago di Compostella. The cathedral in Santiago di Compostella reputedly holds the remains of St. James. Modern day pilgrims are sometimes drawn by faith and sometimes just drawn by the journey itself to trek for a month across the varied landscapes of northern Spain. As an American, Ben did not have a month of vacation but rather than be one of those pilgrims who only traverses the very end of the trail he chose to take on the pilgrim route on a bicycle. Along the way he stayed in the hostels that house the many pilgrims where he met people who had been walking from far beyond the border with France. Through countryside, cities and picturesque towns he rode in both sunshine and driving rain, but the highlight of the trip might not be what you think.

The Amateur Traveler talks to Vicente Perez about his home city of Pamplona, Spain. Pamplona is best known for the festival of San Fermin and of course for the running of the bulls. Vicente tells us more about that colorful festival, of which, the running of the bulls is actually a very small part. From the Txupinazo, the ceremonial firing of a rocket on July 6th until July 14th the population of Pamplona swells with celebrants. Processions of giant figures (the Comparsa), music, bull fights and lots and lots of partying fill the days and nights. But Vicente also tells of some of the other features of Pamplona and the Navarre region that can be enjoyed any time of year including wonderful food, beautiful mountains, castles, and the nearby cave of Zugarramurdi (Known for the Basque witch trials).

The Amateur Traveler talks to Gary Arndt of Everything-Everywhere.com about his recent trip to the Canary Islands. The Canary Islands are tropical volcanic islands that are part of Spain but are located off the coast of Northwestern Africa.They are not named after birds.

    The first thing that struck me when I got off the plane was how much the weather was like Hawaii and I have heard a lot of people call it Europe's Hawaii. It is a part of Spain. There are two provinces that constitute the Canary Islands. One with its capital on Grand Canaria Island and the other on the island of Tenerife. Columbus's last stop over was in the Canary Islands. Each of the islands, and there are 7 main islands, have a very different character.

The Amateur Traveler talks to Photographer Ralph Velasco about two of his favorite cities in Spain, Toledo and Segovia. Both Toledo and Segovia are a short distance from Madrid and both offer interesting and historically significant cities with UNESCO World Heritage sites.

The Amateur Traveler talks to Sherry Ott about Costa Brava Spain. Costa Brava is in Catalonia north of Barcelona on the rugged coastline of Spain. It also extends as far inland as the Pyrenees and includes farming country and medieval towns.

Hear about travel to Extremadura in Spain as the Amateur Traveler talks to Sam from indefiniteadventure.com about living and working there. Extremadura is a lesser known region in southwest Spain on the border with Portugal and adjacent to Andalusia, Madrid and Castilla. Sam lived in the city of Medira for 3 months teaching English.

Hear about travel to the Andalucia region of Spain as the Amateur Traveler talks to Crawford Hill from Chill Expeditions about his adopted home in Southern Spain. Crawford traveled in that area and then fell in love and bought a second home here.

Hear about travel to Seville, Spain as the Amateur Traveler talks to Cat Gaa from sunshineandsiestas.com about life in her adopted city. Hear about what to see, do and eat in Seville. 

Hear about hiking the Camino de Santiago (the Way of St. James) in northern Spain as the Amateur Traveler talks to Linda Martin from The Indie Travel Podcast.

Linda says, "the thing about the Camino is that it is as long as you wanted to be."

Hear about travel to Barcelona, Spain as the Amateur Traveler talks to Lauren Aloise from devourspain.com about traveling to this capital of the region of Catalan. 

Hear about travel to Madrid, Spain as the Amateur Traveler talks to James Blick from DevourTours.com and Yolanda Martin from TheFlamencoGuide.com about their city.

Hear about hiking the Camino Ronda in Northern Spain as the Amateur Traveler talks to Sherry Ott about her trek along the coastline of the Costa Brava region.

Hear about travel to the Basque Region of Spain as the Amateur Traveler talks to David Bumstead about his adopted country and region.

Hear about travel to Valencia, Spain as the Amateur Traveler talks to Jason Jenkins from AnEpicEducation.com about the 2 years his family lived in Spain's 3rd largest city.

The Gothamist is reporting the sad news that Gem Spa, the iconic NY corner store that has been a fixture at St. Marks Place and Second Avenue for around 100 years is being forced to shutter its doors and windows for the last time. The Spa has been struggling to keep up with increasing rent prices and COVID-19 has apparently proven to be the final nail in its coffin.

"It’s where Robert Mapplethorpe bought Patti Smith an egg cream on the day they met. It’s on the back cover of the New York Dolls’ 1973 debut (and where, according to lore, Johnny Thunders and others went for post-heroin sugar fixes between sets at CBGB). Before that, it was where Abbie Hoffman gathered Yippies to rain money on the New York Stock Exchange. It’s where Allen Ginsberg, Ted Berrigan, and other neighborhood poets went to pick up the Sunday New York Times on Saturday nights (and which was inevitably commemorated in their poems)."

Read the rest.

Image: Alex Lozupone, CC BY-SA 4.0 Read the rest

The Vuelta a Espana organisers have confirmed that a race already rescheduled and shortened would also now cut its foray into neighbouring Portugal from the 2020 itinerary.

Other Space Synopsis Other Space is a Science Fiction comedy television series produced by Yahoo that premiered in April of 2015. Created by Paul Feig (Freaks and Geeks) Other Space is set in the early 22nd century and follows the story of the inexperienced crew aboard UMP Cruiser on a routine space exploratory mission. In […]

An eight-year-old boy with good intentions sparked a missing person search on Tuesday.

The following article, Photos Of Kim Jong Un Spark Conspiracy Theories About A Body Double…You Be The Judge, was first published on 100PercentFedUp.com.

For several weeks rumors of the North Korean dictator’s sickness and ultimately, his death, have been making their rounds in the media. North Korea’s state-run media released photos of  Kim Jong Un that were allegedly taken on May 1, at the opening of a fertilizer factory in Sunchon, N. Korea. Twitter users who’ve studied the images are […]

Continue reading: Photos Of Kim Jong Un Spark Conspiracy Theories About A Body Double…You Be The Judge ...

The empty space next to Winnipeg City Hall that once housed restaurants could be turned into a place to help exploited women.

The linear ubiquitin assembly complex (LUBAC) is an essential component of the innate and adaptive immune system. Modification of cellular substrates with linear polyubiquitin chains is a key regulatory step in signal transduction that impacts cell death and inflammatory signaling downstream of various innate immunity receptors. Loss-of-function mutations in the LUBAC components HOIP and HOIL-1 yield a systemic autoinflammatory disease in humans, whereas their genetic ablation is embryonically lethal in mice. Deficiency of the LUBAC adaptor protein Sharpin results in a multi-organ inflammatory disease in mice characterized by chronic proliferative dermatitis (cpdm), which is propagated by TNFR1-induced and RIPK1-mediated keratinocyte cell death. We have previously shown that caspase-1 and -11 promoted the dermatitis pathology of cpdm mice and mediated cell death in the skin. Here, we describe a reciprocal regulation of caspase-1 and LUBAC activities in keratinocytes. We show that LUBAC interacted with caspase-1 via HOIP and modified its CARD domain with linear polyubiquitin and that depletion of HOIP or Sharpin resulted in heightened caspase-1 activation and cell death in response to inflammasome activation, unlike what is observed in macrophages. Reciprocally, caspase-1, as well as caspase-8, regulated LUBAC activity by proteolytically processing HOIP at Asp-348 and Asp-387 during the execution of cell death. HOIP processing impeded substrate ubiquitination in the NF-κB pathway and resulted in enhanced apoptosis. These results highlight a regulatory mechanism underlying efficient apoptosis in keratinocytes and provide further evidence of a cross-talk between inflammatory and cell death pathways.

The CRISPR/Cas9 nucleases have been widely applied for genome editing in various organisms. Cas9 nucleases complexed with a guide RNA (Cas9–gRNA) find their targets by scanning and interrogating the genomic DNA for sequences complementary to the gRNA. Recognition of the DNA target sequence requires a short protospacer adjacent motif (PAM) located outside this sequence. Given that the efficiency of target location may depend on the strength of interactions that promote target recognition, here we sought to compare affinities of different Cas9 nucleases for their cognate PAM sequences. To this end, we measured affinities of Cas9 nucleases from Streptococcus pyogenes, Staphylococcus aureus, and Francisella novicida complexed with guide RNAs (gRNAs) (SpCas9–gRNA, SaCas9–gRNA, and FnCas9–gRNA, respectively) and of three engineered SpCas9–gRNA variants with altered PAM specificities for short, PAM-containing DNA probes. We used a “beacon” assay that measures the relative affinities of DNA probes by determining their ability to competitively affect the rate of Cas9–gRNA binding to fluorescently labeled target DNA derivatives called “Cas9 beacons.” We observed significant differences in the affinities for cognate PAM sequences among the studied Cas9 enzymes. The relative affinities of SpCas9–gRNA and its engineered variants for canonical and suboptimal PAMs correlated with previous findings on the efficiency of these PAM sequences in genome editing. These findings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome-editing efficiency.

4 March 2020 , Volume 96, Number 2

Members Event Webinar

Carmen Gonsalves, Head, International Cyber Policy, Ministry of Foreign Affairs, Netherlands
Suzanne Spaulding, Senior Adviser for Homeland Security, Center for Strategic and International Studies
Chair: Joyce Hakmeh, Senior Research Fellow, International Security Programme and Co-Editor, Journal of Cyber Policy, Chatham House

7 May 2020 , Volume 96, Number 3

Members Event Webinar

Carmen Gonsalves, Head, International Cyber Policy, Ministry of Foreign Affairs, Netherlands
Suzanne Spaulding, Senior Adviser for Homeland Security, Center for Strategic and International Studies
Chair: Joyce Hakmeh, Senior Research Fellow, International Security Programme and Co-Editor, Journal of Cyber Policy, Chatham House

Members Event Webinar

Carmen Gonsalves, Head, International Cyber Policy, Ministry of Foreign Affairs, Netherlands
Suzanne Spaulding, Senior Adviser for Homeland Security, Center for Strategic and International Studies
Chair: Joyce Hakmeh, Senior Research Fellow, International Security Programme and Co-Editor, Journal of Cyber Policy, Chatham House

7 May 2020 , Volume 96, Number 3

The CRISPR/Cas9 nucleases have been widely applied for genome editing in various organisms. Cas9 nucleases complexed with a guide RNA (Cas9–gRNA) find their targets by scanning and interrogating the genomic DNA for sequences complementary to the gRNA. Recognition of the DNA target sequence requires a short protospacer adjacent motif (PAM) located outside this sequence. Given that the efficiency of target location may depend on the strength of interactions that promote target recognition, here we sought to compare affinities of different Cas9 nucleases for their cognate PAM sequences. To this end, we measured affinities of Cas9 nucleases from Streptococcus pyogenes, Staphylococcus aureus, and Francisella novicida complexed with guide RNAs (gRNAs) (SpCas9–gRNA, SaCas9–gRNA, and FnCas9–gRNA, respectively) and of three engineered SpCas9–gRNA variants with altered PAM specificities for short, PAM-containing DNA probes. We used a “beacon” assay that measures the relative affinities of DNA probes by determining their ability to competitively affect the rate of Cas9–gRNA binding to fluorescently labeled target DNA derivatives called “Cas9 beacons.” We observed significant differences in the affinities for cognate PAM sequences among the studied Cas9 enzymes. The relative affinities of SpCas9–gRNA and its engineered variants for canonical and suboptimal PAMs correlated with previous findings on the efficiency of these PAM sequences in genome editing. These findings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome-editing efficiency.

Invitation Only Research Event