We investigated dose-fractionated polymyxin B (PB) on acute kidney injury (AKI). PB at 12 mg of drug/kg of body weight per day (once, twice, and thrice daily) was administered in rats over 72 h. The thrice-daily group demonstrated the highest KIM-1 increase (P = 0.018) versus that of the controls (P = 0.99) and histopathological damage (P = 0.013). A three-compartment model best described the data (bias, 0.129 mg/liter; imprecision, 0.729 mg²/liter²; R², 0.652,). Area under the concentration-time curve at 24 h (AUC₂₄) values were similar (P = 0.87). The thrice-daily dosing scheme resulted in the most PB-associated AKI in a rat model.

Chromosomal resistance islands containing the methicillin resistance gene mecD (McRI_mecD) have been reported in Macrococcus caseolyticus. Here, we identified novel macrolide resistance genes in Macrococcus canis on similar elements, called McRI_msr. These elements were also integrated into the 3' end of the 30S ribosomal protein S9 gene (rpsI), delimited by characteristic attachment (att) sites, and carried a related site-specific integrase gene (int) at the 5' end. They carried novel macrolide resistance genes belonging to the msr family of ABC subfamily F (ABC-F)-type ribosomal protection protein [msr(F) and msr(H)] and the macrolide efflux mef family [mef(D)]. Highly related mef(D)-msr(F) fragments were found on diverse McRI_msr elements in M. canis, M. caseolyticus, and Staphylococcus aureus. Another McRI_msr-like element identified in an M. canis strain lacked the classical att site at the 3' end and carried the msr(H) gene but no neighboring mef gene. The expression of the novel resistance genes in S. aureus resulted in a low-to-moderate increase in the MIC of erythromycin but not streptogramin B. In the mef(D)-msr(F) operon, the msr(F) gene was shown to be the crucial determinant for macrolide resistance. The detection of circular forms of McRI_msr and the mef(D)-msr(F) fragment suggested mobility of both the island and the resistance gene subunit. The discovery of McRI_msr in different Macrococcus species and S. aureus indicates that these islands have a potential for dissemination of antibiotic resistance within the Staphylococcaceae family.

Summary:

The first basket clinical trial testing the BRAF inhibitor vemurafenib resulted in evidence of activity in 13 unique cancer types with BRAF^V600 mutations, but the response rates were variable. Therefore, different cancer histologies with the same driver oncogene display different degrees of oncogenic pathway addiction.

See related article by Subbiah et al., p. 657.

Slayin 2 is a sequel done right. It takes the fast-paced, score-based 2D action of the iOS/Android original and inserts it into an RPG framework with multiple classes, upgrades, and secrets. Then it adds a layer of charming pixel art and plugs in some rocking tunes courtesy of Matt Creamer (Retro City Rampage). Not everything is perfect — a few difficulty spikes require a bit of grinding and the twitchy combat mechanics aren't especially deep — but overall Slayin 2 represents a smooth and successful transition from mobile to console.

There's a story in Slayin 2, but developers FDG Entertainment and Pixel Licker don't tell it with much urgency. Monsters have sacked the kingdom's castle... the royal family is missing... there's something about a coven of witches. It's all background noise, until after the final boss battle. Only then do we understand what was going on and the high stakes. It's a somewhat backward way of storytelling, but never mind; discovering new areas, finding secrets, and unlocking new gear will push you forward regardless.

Slayin 2 is divided into two modes: Story and Arcade. The latter is based on the endless rogue-like mobile game. You'll select from eight total heroes, then fight your way through waves of enemies and bosses, leveling up and collecting treasure — which a traveling shopkeeper will accept for new gear and other boons. If you die, the game records your high score and kicks you back to the very beginning. Had Slayin 2 included only this mode, it would be a passable experience.

Luckily, there's Story mode. While it employs the same level layouts and mechanics of Arcade mode, it adds a story, a town filled with shops and quest-giving NPCs, branching paths, and secret stages. Several of the town's residents provide riddles which may lead you to a hidden object or a secret exit. Others will join your crusade (and become playable characters) for a price. Still others will sell you charms, new weapons, or enhance your "spark" bar, which allows powerful special attacks. If you score well enough in the game's many stages, a local bard will even write a song in your honor. This RPG framework elevates the Slayin formula. It gives you purpose and direction, and things to ponder outside hacking and slashing.

This is essential to the success of Slayin 2, because its gameplay loop can be simple and grindy. As in the original iOS/Android game, here you'll take down enemies simply by running into them. By pushing left or right you'll extend your weapon — sword, mace, lance, magic orb, bow and arrow, etc. — and by making contact with a monster you'll do damage. It's an uncomplicated set-up that surely owes something to its mobile origins. FDG and Pixel Licker add plenty of enemy variety and special moves unique to each hero in order to keep things interesting, but the twitchy, left-right-left-right combat remains.

Slayin 2 isn't totally without strategy however. The development teams have mixed up the action with dual planes, so that players can jump from foreground to background by pressing the R button. This makes the momentary gameplay more dynamic and allows players to chain together combos and avoid enemy contact by bouncing back and forth between each plane. It's especially useful for boss fights (try facing a boss in Arcade mode with only one plane enabled and you'll see).

Speaking of bosses, they're all unique and present tricky-but-identifiable attack patterns. You'll start with a giant green slime and graduate to tree monsters, minotaurs, killer plants, and gargantuan crabs, among many others. You may see some more than others, as a handful of stages are unduly difficult. You'll need to replay easier levels multiple times to earn enough treasure to buy weapon upgrades and charms, which will allow you to survive in the more daunting levels. This irksome need to grind begins to evaporate towards the end of Slayin 2, as you amass a fortune and max out your weapons, but it does flavor the first third of the game.

If you do hit a wall, you can always invite a friend in couch co-op to share the burden. Local multiplayer is a great addition to the franchise, although it seems like Slayin 2 was balanced with a single player in mind.

Graphically, Slayin 2 represents a noticeable improvement over the premier mobile game. Its charming pixel art leaps off the screen and its detailed backgrounds grant each level its own personality. The driving soundtrack by Matt Creamer also stands out, thanks to heroic tunes and plenty of anachronistic electric guitar riffs that pair well with the game's non-stop action.

FDG and Pixel Licker could have replicated the original Slayin experience, slapped on a new coat of paint, published on Switch, and called it a day. Instead the studio transformed the rogue-like template into a more substantial role-playing adventure complete with story, side-quests, secrets, unlockable characters, and upgrades aplenty. The core combat mechanic still lacks nuance and a handful of difficulty spikes slow the game down, but in toto Slayin 2 is a solid sequel.

Full Article - https://www.vgchartz.com/article/443336/slayin-2-ns/

An AI based on deepfake technology can translate videos of a person speaking in one language into another. In future, it could help people who don’t speak the same language communicate

Watford chairman Scott Duxbury has questioned whether the Premier League should return amid the coronavirus pandemic.

She’s also learning to iron.

A winner is you! You won’t feel asleep as you listen to this week’s show. As we prep for PAX, Alex tells us about Sword...

Audio of a private conversation shows the 44th president's unvarnished views about the former national security adviser's case and the White House's COVID-19 response.

The remains of three slaves found in Mexico contain the earliest signs of the hepatitis B virus and yaws bacteria in the Americas, suggesting transatlantic slavery introduced these diseases

The top Republican in the U.S. House of Representatives called for a pause in coronavirus legislation on Thursday, saying Congress should first assess the impact of earlier relief bills and then take a slower path forward through committee hearings.

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Tesla is known for it’s electric inventions. Specifically, in the...

The post Tesla Wants To Be UK’s Electricity Provider appeared first on Man's World India.

Former President Barack Obama slammed the Justice Department for abandoning its prosecution of President Trump's ex-national security adviser Michael Flynn, saying it puts "the rule of law is at risk."

Mr. Obama's comments came Friday night while privately talking to ex-members of his administration. A tape of the conversation was ...

Tesla Inc has not been allowed to resume operations at its California vehicle factory as local lockdown measures to curb the spread of the coronavirus remain in effect, a county health official said on Friday.

Electric carmaker Tesla Inc has entered into an agreement for a working capital loan of up to 4 billion yuan ($565.51 million) with a lender from China for its Shanghai car plant, according to a regulatory filing on Friday.

Tesla Inc "must not reopen" its vehicle factory in the San Francisco Bay area as local lockdown measures to curb the spread of the coronavirus remain in effect, the local county health department said on Friday.

Nigerian comedian Maryam Apaokagi has a sure-fire way of getting people to listen to her coronavirus health advice - she delivers it with a hard slap in the face.

Tesla Inc "must not reopen" its vehicle factory in the San Francisco Bay area as local lockdown measures to curb the spread of the coronavirus remain in effect, the local county health department said on Friday.

Lorry driver Maurice Robinson has admitted the manslaughter of 39 people who were found dead in a refrigerated trailer in Essex.

Follow our live coronavirus updates here Coronavirus: the symptoms

Coronavirus: the symptoms

Florida judge Dennis Bailey said it was "remarkable" that many attorneys appear inappropriately on camera for hearings using the Zoom video conferencing app.

Follow our live coronavirus updates here Coronavirus: the symptoms

Officials in San Clemente draw line in the sand at local park after weeks of rule-breaking by unruly skateboarders

A man has been charged with manslaughter in connection with the deaths of 39 migrants found in a refrigerated lorry container in Essex.

A couple who were living their dream of sailing across the Atlantic have shared their "shock" of returning to land and hearing the news about the global coronavirus pandemic.

Labour has proposed legislation to deliver a dedicated fast-tracked fund for domestic abuse services affected by the coronavirus crisis.

Piers Morgan slammed a Home Office minister and accused her of laughing as he branded PPE shortages a "national disgrace" in a furious exchange on Good Morning Britain.

Elon Musk caused shares in his company Tesla to drop more than 11 per cent after tweeting that the price is "too high".

A woman in her 20s is fighting for her life in hospital with "slash injuries" after a street fight in north-west London.

Care home workers say employers are playing "Russian roulette" with their lives as personal protective equipment (PPE) is withheld or rationed amid unclear guidance, according to a union.

Tesco has been slammed after putting clothing on a half price sale in stores despite lockdown rules banning non-essential shopping.

They are among the earliest enslaved African people brought to the Americas.

From Tashkent to Samarkand and Bukhara, travel writer Caroline Eden believes Uzbekistan offers a dazzling mix of traditional style and a modern outlook

Twenty five years after the fall of the USSR, it’s interesting how the Soviet-era hangover lingers in Uzbekistan. Hulking apartment blocks are gradually being upgraded, and while you won’t spot statues of Lenin (they’ve been replaced by the nomadic conqueror Tamerlane and celebrated medic Ibn-Sina) you will see plenty of samovars (Russian kettles) and Soviet military medals for sale in the markets. But you will also see master ikat weavers reviving weaving traditions, and many musicians and artists are now turning to their Islamic heritage for influence. This mix of Soviet legacy and Uzbek Islam is one of the things that makes the country so fascinating.

Data can be retrieved even after owners perform a factory reset, researcher says.

The company has submitted an application to the Gas and Electricity Markets Authority "authorising it to generate electricity"

I'm surprised this hasn't been mentioned yet since its been going on for a few days now.  Great game.  Price before GCU.

Slay the Spire (PS4) - $8.99

https://www.bestbuy.com/site/slay-the-spire-standard-edition-playstation-4/6379879.p?skuId=6379879

Slay the Spire (Switch) - $21.99

https://www.bestbuy.com/site/slay-the-spire-standard-edition-nintendo-switch/6379876.p?skuId=6379876

Kim Kardashian shared a throwback photo of her mom looking amazing in a yellow-colored bikini, Kris Jenner (64), from the ’80s. The photo was taken when Kris was 33 years old, just after she gave birth to Robert Jr. Kim wanted to pay tribute to “the best mother in the entire world” Kris for Mother’s […]

The post Kim Kardashian Slammed For Message She Send To Kris Jenner On Mother appeared first on Chart Attack.

Their interview with "James Corden" wasn't what it seemed...

Not once, but twice

From Creekside: The B.C. provincial government is trying to green light the construction of a massive LNG terminal on Lelu Island in the Skeena Estuary — Pacific Northwest LNG, backed by Malaysian energy giant Petronas — without the consent of the people who rejected a $1.15 billion dollar deal from Petronas to gain that consent. […]

From 'Saturday Night Live' to the big screen, Pete Davidson could become Judd Apatow's next comedy megastar.

Sean S. Cunningham's slasher 'Friday the 13th' is celebrating its 40th anniversary this week. Its effect on the horror genre defined the 1980s.