CES 2014, LAS VEGAS – HARMAN, the premium global audio and infotainment group (NYSE:HAR) debuted several new products for the mobile aftermarket today at CES, continuing its tradition of leadership for best-in-class car audio.

NEW YORK INTERNATIONAL AUTO SHOW – HARMAN, the premium global audio and infotainment group (NYSE:HAR), is making a strong showing at this year’s New York International Auto Show, with a range of leading automakers featuring HARMAN branded audio systems in vehicles debuting at the show. In addition to the new vehicles– which run the gamut from modern sedans to sports cars, minivans and crossover SUVs – HARMAN technology and solutions can be found in automaker booths throughout the show, a reflection of HARMAN’s longstanding auto partnerships and unmatched industry achievements.

Shanghai Auto Show 2019 – SHANGHAI – April 16, 2019 – At the Shanghai Auto Show, HARMAN International, a wholly-owned subsidiary of Samsung Electronics Co., Ltd. focused on connected technologies for automotive, consumer and enterprise markets, announced...

The desire to get things ‘my way’ is innately human. From suggested playlists on Spotify to personalized recommendations on Netflix, consumers love it when things are tailored to their own preferences. The concept of personalization isn’t new but the...

Each year, Americans collectively spend 70 billion hours behind the wheel. Three out of four Americans listen to audio in the car – and get this – half of all listening time occurs in the car, according to Edison Research Share of Ear 2018.   It is no...

Studies show that listening to music can elevate your mood, reduce stress, ease pain, fend off depression, and even improve post-surgery outcomes. So if you are looking for a simple way to uplift your mood on any given day – just sit back, listen and cue...

GUJARAT, INDIA – HARMAN (NYSE:HAR), the premier connected technologies company for automotive, consumer and enterprise markets, today opened an exclusive premium showroom for its high-end, Luxury Audio range of products in Ahmedabad, Gujarat. This is the company’s fifth luxury audio showroom* in India and offers the most advanced high-performance audio systems available in the country.

BENGALURU, INDIA – HARMAN International Industries, Incorporated (NYSE: HAR), the premier connected technologies company for automotive, consumer and enterprise markets, today announced that it has opened a new center of competence (CoC) for HARMAN’s...

NORTHRIDGE, Calif.—HARMAN Professional Solutions, the global leader in audio, video, lighting and control systems, today announced new AKG K361-BT and K371-BT professional studio headphones featuring Bluetooth connectivity at the 2020 NAMM show in...

The car is expected to change more in the next five years than it has in the last fifty. What will the future of automotive audio look like? What challenges will suppliers and OEMs alike face in the coming years? At the upcoming International Conference...

In the midst of the Covid-19 pandemic and the drastic changes in social interaction that it has caused, who among us has not felt the desire to reach out, to engage, to reconnect? At HARMAN, one of our most dearly-held beliefs is that music empowers...

Palo Alto, CA – Aha™ by HARMAN today announced that the new 2014 Mazda3 to be launched in Japan, the United States, and a number of additional North American and Asian Countries this fall will offer access to the Aha free service with the ability to select presets from more than 40,000* audio and information stations from Japan and other countries.

LOS ANGELES, CA – HARMAN International Industries, Inc. (NYSE:HAR), the premium audio and infotainment group, will have a major presence at the 137th Audio Engineering Society Convention as it returns to the Los Angeles Convention Center Thursday, October 9 through Sunday, October 12 for the first time in twelve years. HARMAN, the premiere sponsor of both the annual AES President’s Reception and the AES Student Zone, will present several research papers, highlight new Professional audio equipment, and demonstrate the company’s latest innovative music restoration technology, Clari-Fi, throughout the week.

LOS ANGELES, CA – HARMAN (NYSE: HAR), the premier global audio and infotainment group, is making its mark at this year’s Los Angeles Auto Show with several leading automakers debuting vehicles that feature HARMAN branded audio systems. With brands like Harman Kardon, JBL, Infinity, Mark Levinson and Lexicon, HARMAN technology and solutions can be found on automaker stands throughout the show -- a reflection of the company’s strong, longstanding automotive OEM partnerships and industry-leading audio innovation.

DEARBORN, MI – The Lincoln Motor Company announced an exclusive 10-year collaboration with Revel® that will add the premium loudspeaker brand to Lincoln vehicles. The pairing will make Lincoln the only automotive brand to offer Revel audio systems.

IFA 2015, BERLIN – HARMAN International Industries, Incorporated (NYSE:HAR), the premier connected technologies company for automotive, consumer and enterprise markets, today announced the world’s first aftermarket amplifiers to feature HARMAN’s Clari-Fi...

NEW YORK AUTO SHOW – March 23, 2016 – HARMAN International Industries, Incorporated (NYSE:HAR), the premier connected technologies company for automotive, consumer and enterprise markets, will join leading global automakers at the 2016 New York Auto Show...

From their conception to the 1950’s, automobiles were strictly pieces of analog machinery. It seemed they were able to connect people physically by transporting them from place to place, but failed to connect with them at an emotional level. But as the...

U.S. brokers should not be forced to sign an agreement that could make them liable for breaches of a massive new industry trading database that they have no control over, a leading financial industry...

Human Rights Watch (HRW) said on Saturday that Saudi Arabian authorities recently detained and are holding incommunicado Prince Faisal bin Abdullah, who had previously been netted in an anti-corruption drive and released in late 2017.

Music has always been an important part of human culture, dating all the way back to ancient civilizations. Archeologists have unearthed harps in Egypt and primitive trumpets in Rome, as well as Grecian vases and tablets illustrating the presence of...

HARMAN is excited and ready to head to Las Vegas in the New Year, once again, for the Consumer Electronics Show or CES. Without a doubt, CES is one of the most prominent and strategic events for the technology, automotive and the broader business...

CES 2019, LAS VEGAS – JANUARY 7, 2019 – Today’s discerning audio consumer is seeking sophisticated design and innovative technology that seamlessly blends with their daily lives. When it comes to a multi-room speaker system that means: ease of use, form...

At this year’s North American International Auto Show (NAIAS) in Detroit, HARMAN and its automotive partners brought the latest evolution of premium in-car experiences that reflect and seamlessly connect with your lifestyle. Ensuring users are...

Companies across the globe are focused on delivering voice-enabled products to their customers. However, voice technology is a nascent, complicated one that few companies can develop internally, without any collaboration. Enter, HARMAN Embedded Audio. A...

HARMAN is gearing up to present its best-in-class audio technologies and solutions for many of the world’s foremost automotive OEMs at IAA 2019 from September 12-22 in Frankfurt/Main. Now in its 68th edition, IAA is the world’s largest motor show as well...

CES 2020 – LAS VEGAS – JANUARY 6, 2020 – Today, Harman Kardon announced the latest speaker to join its sophisticated icons collection, the Harman Kardon Aura Studio 3. This dome-shaped home audio combines style and function, while delivering 360-degree...

CES 2020 – LAS VEGAS, NV – JANUARY 6, 2020 – Following the Harman Kardon Citation Series announcement last year, the brand has expanded its wireless speaker series with additional form factors for every room and living space. Outfitted with Harman...

This week, watch as Westworld breaks out of the park and into LA, discover why vision is so important and listen as a drama exploits the weirdness of sound

With social media, newspapers and politicians all espousing unverified covid-19 findings, use these seven signs to tell if a study should be treated with caution

CES 2020, LAS VEGAS – JANUARY 6, 2020 – Harman International, a wholly-owned subsidiary of Samsung Electronics Co., Ltd. focused on connected technologies for automotive, consumer and enterprise markets, today announced its EV Plus+ Solutions, a new...

Are you a human, or a human-Neanderthal hybrid? The concept of the species, one of the most basic in biology, may not be as well-defined as we think

Two more firms are eyeing a share of Reliance Industries Ltd's $65-billion digital unit Jio Platforms, according to Bloomberg News, setting them up to be a part of a growing list of firms that have recently invested in the Indian company.

A series of studies of the genomes of thousands of samples of the new coronavirus SARS-CoV-2 show that it is mutating and evolving as it adapts to its human hosts. Soraya Ali reports.

With social media, newspapers and politicians all espousing unverified covid-19 findings, use these seven signs to tell if a study should be treated with caution

Title: More Studies Link Vaping to Asthma, COPD
Category: Health News
Created: 1/14/2020 12:00:00 AM
Last Editorial Review: 1/14/2020 12:00:00 AM

Title: Studies Point to Reasons for Mammograms in 40s
Category: Health News
Created: 5/1/2012 11:00:00 AM
Last Editorial Review: 5/1/2012 12:00:00 AM

A deluge of new data does not suggest harm with ACE inhibitors and angiotensin blockers in COVID-19 rates or outcomes but suggests possible differential effects of the two drug classes.

Title: Did the Movie 'Joker' Reinforce Prejudice Against Mentally Ill?
Category: Health News
Created: 4/27/2020 12:00:00 AM
Last Editorial Review: 4/28/2020 12:00:00 AM

ABSTRACT

Transporters belonging to the chromosomally encoded resistance-nodulation-division (RND) superfamily mediate multidrug resistance in Gram-negative bacteria. However, the cotransfer of large gene clusters encoding RND-type pumps from the chromosome to a plasmid appears infrequent, and no plasmid-mediated RND efflux pump gene cluster has yet been found to confer resistance to tigecycline. Here, we identified a novel RND efflux pump gene cluster, designated tmexCD1-toprJ1, on plasmids from five pandrug-resistant Klebsiella pneumoniae isolates of animal origin. TMexCD1-TOprJ1 increased (by 4- to 32-fold) the MICs of tetracyclines (including tigecycline and eravacycline), quinolones, cephalosporins, and aminoglycosides for K. pneumoniae, Escherichia coli, and Salmonella. TMexCD1-TOprJ1 is closely related (64.5% to 77.8% amino acid identity) to the MexCD-OprJ efflux pump encoded on the chromosome of Pseudomonas aeruginosa. In an IncFIA plasmid, pHNAH8I, the tmexCD1-toprJ1 gene cluster lies adjacent to two genes encoding site-specific integrases, which may have been responsible for its acquisition. Expression of TMexCD1-TOprJ1 in E. coli resulted in increased tigecycline efflux and in K. pneumoniae negated the efficacy of tigecycline in an in vivo infection model. Expression of TMexCD1-TOprJ1 reduced the growth of E. coli and Salmonella but not K. pneumoniae. tmexCD1-toprJ1-positive Enterobacteriaceae isolates were rare in humans (0.08%) but more common in chicken fecal (14.3%) and retail meat (3.4%) samples. Plasmid-borne tmexCD1-toprJ1-like gene clusters were identified in sequences in GenBank from Enterobacteriaceae and Pseudomonas strains from multiple continents. The possibility of further global dissemination of the tmexCD1-toprJ1 gene cluster and its analogues in Enterobacteriaceae via plasmids may be an important consideration for public health planning.

IMPORTANCE In an era of increasing concerns about antimicrobial resistance, tigecycline is likely to have a critically important role in the treatment of carbapenem-resistant Enterobacteriaceae, the most problematic pathogens in human clinical settings—especially carbapenem-resistant K. pneumoniae. Here, we identified a new plasmid-borne RND-type tigecycline resistance determinant, TMexCD1-TOprJ1, which is widespread among K. pneumoniae isolates from food animals. tmexCD1-toprJ1 appears to have originated from the chromosome of a Pseudomonas species and may have been transferred onto plasmids by adjacent site-specific integrases. Although tmexCD1-toprJ1 still appears to be rare in human clinical isolates, considering the transferability of the tmexCD1-toprJ1 gene cluster and the broad substrate spectrum of TMexCD1-TOprJ1, further dissemination of this mobile tigecycline resistance determinant is possible. Therefore, from a "One Health" perspective, measures are urgently needed to monitor and control its further spread. The current low prevalence in human clinical isolates provides a precious time window to design and implement measures to tackle this.

ABSTRACT

The profiling of gene expression by RNA sequencing (RNA-seq) has enabled powerful studies of global transcriptional patterns in all organisms, including bacteria. Because the vast majority of RNA in bacteria is rRNA, it is standard practice to deplete the rRNA from a total RNA sample such that the reads in an RNA-seq experiment derive predominantly from mRNA. One of the most commonly used commercial kits for rRNA depletion, the Ribo-Zero kit from Illumina, was recently discontinued abruptly and for an extended period of time. Here, we report the development of a simple, cost-effective, and robust method for depleting rRNA that can be easily implemented by any lab or facility. We first developed an algorithm for designing biotinylated oligonucleotides that will hybridize tightly and specifically to the 23S, 16S, and 5S rRNAs from any species of interest. Precipitation of these oligonucleotides bound to rRNA by magnetic streptavidin-coated beads then depletes rRNA from a complex, total RNA sample such that ~75 to 80% of reads in a typical RNA-seq experiment derive from mRNA. Importantly, we demonstrate a high correlation of RNA abundance or fold change measurements in RNA-seq experiments between our method and the Ribo-Zero kit. Complete details on the methodology are provided, including open-source software for designing oligonucleotides optimized for any bacterial species or community of interest.

IMPORTANCE The ability to examine global patterns of gene expression in microbes through RNA sequencing has fundamentally transformed microbiology. However, RNA-seq depends critically on the removal of rRNA from total RNA samples. Otherwise, rRNA would comprise upward of 90% of the reads in a typical RNA-seq experiment, limiting the reads coming from mRNA or requiring high total read depth. A commonly used kit for rRNA subtraction from Illumina was recently unavailable for an extended period of time, disrupting routine rRNA depletion. Here, we report the development of a "do-it-yourself" kit for rapid, cost-effective, and robust depletion of rRNA from total RNA. We present an algorithm for designing biotinylated oligonucleotides that will hybridize to the rRNAs from a target set of species. We then demonstrate that the designed oligonucleotides enable sufficient rRNA depletion to produce RNA-seq data with 75 to 80% of reads coming from mRNA. The methodology presented should enable RNA-seq studies on any species or metagenomic sample of interest.

Key Points

Background

Water and solute transport across epithelia can occur via the transcellular or paracellular pathways. Tight junctions play a key role in mediating paracellular ion reabsorption in the kidney. In the renal collecting duct, which is a typical absorptive tight epithelium, coordination between transcellular sodium reabsorption and paracellular permeability may prevent the backflow of reabsorbed sodium to the tubular lumen along a steep electrochemical gradient.