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Tyler Perry's Madea's Witness Protection




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Plan proposes $18.7M in funds for AMHS: Alaska House subcommittee advances plan to restore minimal service




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Proboscis Monkey

The proboscis monkey is a species endemic to Borneo. I areas where they occur they are quite easy to encounter as they have reddish fur and make strange burping sounds for communication.




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Crowdsourcing Project Aims to Document the Many U.S. Places Where Women Have Made History

The National Trust for Historic Preservation is looking for 1,000 places tied to women's history, and to share the stories of the figures behind them




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The Long, Fraught History of the Bulletproof Vest

The question of bulletproofing vexed physicians and public figures for years, before pioneering inventors experimented with silk




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The 'Hard Hat Riot' of 1970 Pitted Construction Workers Against Anti-War Protesters

The Kent State shootings further widened the chasm among a citizenry divided over the Vietnam War




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This ‘Health Mirror’ Teaches Proper Handwashing

The smart tool gives users step-by-step instructions based on WHO’s handwashing protocol




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La Loche calls on province to close SLGA liquor store due to COVID-19 outbreak

The village of La Loche is calling on the provincial government to force the closure of liquor stores in the community, which is in the throes of a COVID-19 outbreak. 



  • News/Canada/Saskatoon

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COVID-19 prompts changes to Thunder Bay's Ribfest

Changes are coming to Thunder Bay's popular Ribfest event because of the COVID-19 outbreak, organizers said.



  • News/Canada/Thunder Bay

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Higher rate of testing accomplished than provincial average, says Northwestern Health Unit

It's been more than a week since there were any new or active cases of COVID-19 in the catchment area for the Northwestern Health Unit (NWHU), and staff can not focus increasing testing.



  • News/Canada/Thunder Bay

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Comment on Bebo doesn’t fancy Liz Taylor! by onlinepromoter.info

<span class="topsy_trackback_comment"><span class="topsy_twitter_username"><span class="topsy_trackback_content">Bebo doesn't fancy Liz Taylor! | RSS Feeds – IMC OnAir, India ...: May not do Madhur Bhandarkar's film which appar... http://bit.ly/9btJW3</span></span>




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Comment on Chief Executive Officer – Pro Bono Australia by Rob

Job of The Week: CEO – Nordoff Robbins Music Therapy http://bit.ly/aFIMNM




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Marine Atlantic cancels Argentia run while provincial ferries look to ease restrictions

Demand for service is not expected to recover in the coming weeks, says Marine Atlantic.



  • News/Canada/Nfld. & Labrador

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Aprovechar cada oportunidad

La tripulación de técnicos del Logos Hope sabe que el mantenimiento del barco es una forma de hacer ministerio.




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Province to spend $147 million on northern Alberta communities damaged by floods

Disaster relief funding for punishing spring floods is welcome news to civic leaders in northern Alberta.



  • News/Canada/Edmonton

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Production of Recombinant Adeno-Associated Viruses (rAAVs) by Transient Transfection

The most commonly used method for production of recombinant adeno-associated virus (rAAVs) in research laboratories is by transient triple transfection of 293 cells with AAV cis and trans plasmids and an adenovirus helper plasmid. This protocol describes the processes required to prepare the transfected cell suspension for virus purification.




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Stripping of the Immunoblot for Reprobing

For most immunoblots developed with chemiluminescence or with fluorochrome-based detection systems, it is possible to remove the primary and secondary antibodies from the membrane without affecting the bound antigen. This allows you to reuse the membrane for detection of another protein antigen. The blots developed with chromogenic substrates can also be stripped of antibodies and reprobed, but the bands detected in the first round of immunoblotting will remain unaffected. Stripping and reprobing of the membrane are particularly useful when the amount of sample is limited or when it is important to accurately compare the signal between two different protein antigens in the same sample. Examples of such experiments include determining the levels of a protein antigen in a series of samples relative to the loading control and comparison of the phosphorylated form to the total levels of the protein in the sample.




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Staining the Blot for Total Protein with Ponceau S

Before probing blots for the presence of an antigen, the total composition of the transferred proteins can be determined by staining the nitrocellulose or polyvinylidene fluoride (PVDF) membrane. Staining for proteins is useful to determine the position of the non-prestained molecular weight markers or individual lanes on the gel and to ensure that efficient transfer has occurred. It can be also used to verify equal loading of the samples in the gel when a comparison of the protein of interest between the different samples is important. The conventional procedures such as Coomassie Blue and silver staining methods used for staining polyacrylamide gels are incompatible with immunoblotting. Ponceau S is the more common staining method in immunoblotting protocols because it is compatible with antibody–antigen binding, is cost efficient, and provides a good contrast between the stained bands and background. In this protocol, nitrocellulose or PVDF membrane is rinsed with ultrapure H2O after the transfer of proteins. Ponceau S dye is applied as an acidic aqueous solution, and the proteins on the membrane are stained with red color. The membrane is briefly destained with water and can be photographed or scanned to obtain the image of the total protein staining. Individual lane positions or the molecular weight standards can be marked with a pencil, if required.




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Bradford Assay for Determining Protein Concentration

The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.




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Expected loss provisioning under a global pandemic

FSI Briefs No 3, April 2020. In response to the 2007-09 Great Financial Crisis (GFC), accounting standard setters introduced a new methodology to value loans based on expected credit losses (ECL). The previous approach, based on incurred losses, was viewed as procyclical and inconsistent with prudential objectives. In the wake of the Covid-19 pandemic, several prudential authorities and the Basel Committee on Banking Supervision (BCBS), introduced a series of measures to clarify how banks should consider various public and private debt relief programmes in their ECL estimates and in their calculation of regulatory capital. These measures are intended to incentivise banks to continue supporting the real economy, while reducing pressure on banks' ECL provisions, earnings and regulatory capital. Supervisory initiatives that provide capital relief should be augmented by severe constraints on the payment of dividends, bonuses and share buybacks. These joint actions will simultaneously expand banks' lending capacity and enhance their ability to absorb losses. Prudential authorities face difficult trade-offs as they confront the most severe economic crisis in modern times. Encouraging the use of flexibility in applicable accounting standards, while preserving market trust and transparency in the reported financial statements of banks, will be key in fostering both economic and financial stability.




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Understanding the Pandemic: New Virtual Programs Announced

The National Book Festival Presents series was created to provide a book festival experience to lovers of the event on a year-round basis. Because these programs can no longer be held at the Library, we are offering a virtual multipart series, with authors who have written books about widespread diseases and the worldwide response to them.




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Basel Committee meets to review vulnerabilities and emerging risks, advance supervisory initiatives and promote Basel III implementation

Basel Committee Press release "Basel Committee meets to review vulnerabilities and emerging risks, advance supervisory initiatives and promote Basel III implementationl", 27 February 2020.




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How fintech can promote financial inclusion - a new report on the opportunities and challenges

CPMI Press release "How fintech can promote financial inclusion - a new report on the opportunities and challenges", 14 April 2020




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Basel Committee issues progress report on banks' implementation of the "Principles for effective risk data aggregation and reporting"

BCBS Press release "Basel Committee issues progress report on banks' implementation of the 'Principles for effective risk data aggregation and reporting'", 29 April 2020




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FDA grants emergency approval for first at-home saliva-based coronavirus test

Rutgers' RUCDR Infinite Biologics received an amended emergency use authorization from the FDA late Thursday for the first SARS-CoV-2 coronavirus test that will allow people to collect their own saliva at home and send to a lab for results.




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AGS honors expert and emerging professionals in geriatric care

The American Geriatrics Society annually honors researchers, clinicians, educators, and emerging health professionals who have made outstanding contributions to high-quality, person-centered care for older people.




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Protein shredder in brain cells indirectly regulates fat metabolism

A protein shredder that occurs in cell membranes of brain cells apparently also indirectly regulates the fat metabolism.




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New projections find deconfinement in Montreal could lead to sharp increase in deaths

The current deconfinement plan in the Montreal area could lead to a rapid increase in deaths in the city, according to findings by Quebec's public health institute. 



  • News/Canada/Montreal

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Winnipeg to start testing technology to improve cellular reception, support 5G service

Winnipeg will soon test "small cell" technology to improve cellular reception in parts of Winnipeg, ahead of a possible future transition to 5G service. 



  • News/Canada/Manitoba

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Merchants Now Can List Products on Google Shopping for Free

Merchants soon will be able to sell products on Google Shopping at no charge. Previously, they had to pay per click, but the cost was not fixed. There was no minimum, but they had to set a maximum for ad spend and Google would stop displaying their ads once the maximum was reached. Starting next week, search results on the Google Shopping tab will consist primarily of free product listings.




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Summerside egg plant to cease processing operations in June

Officials with Maritime Pride Eggs say their Summerside, P.E.I., egg facility will cease processing operations on June 5.



  • News/Canada/PEI

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No active cases of COVID-19 in P.E.I., province easing restrictions further

All of P.E.I.'s 27 confirmed cases of COVID-19 are now considered recovered, said Chief Public Health Officer Dr. Heather Morrison.



  • News/Canada/PEI

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International Art Project Seeks To Transform Flint's Image

Artists from Michigan and around the world are painting 50 murals in Flint to refocus the city's image on art rather than the lead-tainted water crisis.




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Ex-Governor's Phone Seized In Flint Water Probe

Authorities investigating Flint's water crisis have used search warrants to seize from storage the state-owned mobile devices of former Gov. Rick Snyder and 65 other current or former officials, The Associated Press has learned.




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Snyder Speaks Out About Search Warrants In Flint Probe

Former Gov. Rick Snyder says news coverage about search warrants being used to get his state-issued cellphone and computer from government storage in the Flint water investigation is "very sloppy and misleading."




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Flint Hears From Prosecutors Who Dropped Water Charges

Prosecutors who dropped charges against eight people in the Flint water scandal explained their decision in a public forum Friday night, telling frustrated, shocked and saddened residents they must look at hundreds of mobile devices and millions of documents that a previous investigative team never reviewed.




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Ex-FBI Agent Defends Flint Water Probe After Criticism

A former FBI agent who investigated the Flint water scandal says the team was moving toward new charges when new prosecutors took over.




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Childhood Well-Being In Flint Improving After Water Crisis

The Flint Water Crisis captured national attention about the dangers of lead contamination in public water supplies. Now, nearly six years after the problem was first detected, health officials are reporting on the well-being of children in Flint.




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Residential property prices: selected series (nominal and real)

Global real residential property prices rose 1.4% year/year in aggregate in Q3 2019, reflecting subdued developments both in advanced (+ 1.5%) and emerging market economies (+ 1.3%).




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Fehmi Mehmeti: The Central Bank of the Republic of Kosovo has taken steps to protect the economy from Covid-19 damages

Speech by Mr Fehmi Mehmeti, Governor of the Central Bank of the Republic of Kosovo, at the press conference where the details of the measures taken by the CBK for maintaining health in the economy were given, Pristina, 3 April 2020.




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A New Kind of Productive: Working Remotely on the Performing Arts Special Collections of the Library of Congress

Vincent Novara, Head of the Music Division's Acquisitions & Processing Section, describes his section's new teleworking practices during a global pandemic.




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RJ Barrett providing 200,000 meals through Mississauga Food Bank

The Mississauga Food Bank says a $100,000 donation made earlier this week by New York Knicks rookie RJ Barrett will provide 200,000 meals as part of the community's fight against the COVID-19 pandemic.



  • Sports/Basketball/NBA

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International restrictions pose a challenge for resuming pro sports

As the NHL, NBA and MLB consider resuming play, the issue of international travel poses another hurdle for Canadian-based teams.





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Jharkhand bans 11 brands of pan masala for one year to protect public health – Zee News

Jharkhand bans 11 brands of pan masala for one year to protect public health  Zee NewsJharkhand bans 11 pan-masala brands on health concerns  Hindustan TimesJharkhand Bans 11 Pan Masala Brands for One Year to Protect Public Health&n...



  • IMC News Feed


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Cyber Criminals Conduct Business Email Compromise through Exploitation of Cloud-Based Email Services, Costing US Businesses More Than $2 Billion




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Toronto FC's home becomes a massive kitchen to bolster meal program

In the first two weeks of the operation, the sports organization's meal program has used almost 25,000 pounds of chicken, 15,000 pounds of potatoes, 10,000 pounds of mixed vegetables and 8,000 pounds of pasta.



  • News/Canada/Toronto

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COVID-19 numbers from provincial, First Nations data sharing agreement won't be made public without consent

Manitoba health officials have an agreement with First Nations leaders to track and share COVID-19 data, but the public may never know specifics of what the unique agreement yields.



  • News/Canada/Manitoba

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Supporters propose changes to N.B. motor vehicle act to honour Brady Francis

Friends and family of Brady Francis, a 22-year-old man who died in 2018 after a hit-and-run collision, are proposing changes to make reporting vehicle collisions with wildlife mandatory in New Brunswick.