(Universitat Autonoma de Barcelona) More than 500 researchers from all around the world will gather virtually tomorrow Wednesday May 6 at the Institute of Environmental Science and Technology of the Universitat Autònoma de Barcelona (ICTA-UAB) to discuss and propose how society should adopt more sustainable and low-carbon forms of lifestyle that contribute to mitigating climate change.

Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the PGK1-catalyzed forward reaction. The Km values for 1,3-bisphosphoglyceric acid (1,3-BPG, the forward reaction substrate) were 4.36 μm (yeast PGK1) and 6.86 μm (human PKG1). The Km values for 3-phosphoglycerate (3-PG, the reverse reaction substrate and a serine precursor) were 146 μm (yeast PGK1) and 186 μm (human PGK1). The Vmax of the forward reaction was about 3.5- and 5.8-fold higher than that of the reverse reaction for the human and yeast enzymes, respectively. Consistently, the intracellular steady-state concentrations of 3-PG were between 180 and 550 μm in cancer cells, providing a basis for glycolysis to shuttle 3-PG to the serine synthesis pathway. Using siRNA-mediated PGK1-specific knockdown in five cancer cell lines derived from different tissues, along with titration of PGK1 in a cell-free glycolysis system, we found that the perturbation of PGK1 had no effect or only marginal effects on the glucose consumption and lactate generation. The PGK1 knockdown increased the concentrations of fructose 1,6-bisphosphate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, and 1,3-BPG in nearly equal proportions, controlled by the kinetic and thermodynamic states of glycolysis. We conclude that perturbation of PGK1 in cancer cells insignificantly affects the conversion of glucose to lactate in glycolysis.

2018 lands with a distinct thud as Charlotte Jee tackles Meltdown and Spectre, David Price wrestles with Apple's batteries and its new iMac Pro, before Miriam Harris works through the Bafta nominations for visual effects. Henry Burrell leads us down the rabbit hole.

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7 May 2020 , Volume 96, Number 3

The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs.

Branched chain amino acids (BCAAs) are associated with the progression of obesity-related metabolic disorders, including T2DM and non-alcoholic fatty liver disease. However, whether BCAAs disrupt the homeostasis of hepatic glucose and lipid metabolism remains unknown. In this study, we observed that BCAAs supplementation significantly reduced high-fat (HF) diet-induced hepatic lipid accumulation while increasing the plasma lipid levels and promoting muscular and renal lipid accumulation. Further studies demonstrated that BCAAs supplementation significantly increased hepatic gluconeogenesis and suppressed hepatic lipogenesis in HF diet-induced obese (DIO) mice. These phenotypes resulted from severe attenuation of Akt2 signaling via mTORC1- and mTORC2-dependent pathways. BCAAs/branched-chain α-keto acids (BCKAs) chronically suppressed Akt2 activation through mTORC1 and mTORC2 signaling and promoted Akt2 ubiquitin-proteasome-dependent degradation through the mTORC2 pathway. Moreover, the E3 ligase Mul1 played an essential role in BCAAs/BCKAs-mTORC2-induced Akt2 ubiquitin-dependent degradation. We also demonstrated that BCAAs inhibited hepatic lipogenesis by blocking Akt2/SREBP1/INSIG2a signaling and increased hepatic glycogenesis by regulating Akt2/Foxo1 signaling. Collectively, these data demonstrate that in DIO mice, BCAAs supplementation resulted in serious hepatic metabolic disorder and severe liver insulin resistance: insulin failed to not only suppress gluconeogenesis but also activate lipogenesis. Intervening BCAA metabolism is a potential therapeutic target for severe insulin-resistant disease.

Hepatic steatosis, the excess storage of intrahepatic lipids, is a rampant clinical problem associated with the obesity epidemic. Hepatic steatosis is linked to increased risk for insulin resistance, type 2 diabetes, and cardiovascular and advanced liver disease. Accumulating evidence shows that physical activity, exercise, and aerobic capacity have profound effects on regulating intrahepatic lipids and mediating susceptibility for hepatic steatosis. Moreover, exercise can effectively reduce hepatic steatosis independent of changes in body mass. In this perspective, we highlight 1) the relationship between obesity and metabolic pathways putatively driving hepatic steatosis compared with changes induced by exercise; 2) the impact of physical activity, exercise, and aerobic capacity compared with caloric restriction on regulating intrahepatic lipids and steatosis risk; 3) the effects of exercise training (modalities, volume, intensity) for treatment of hepatic steatosis, and 4) evidence for a sustained protection against steatosis induced by exercise. Overall, evidence clearly indicates that exercise powerfully regulates intrahepatic storage of fat and risk for steatosis.

The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs.

7 May 2020 , Volume 96, Number 3

14 January 2020

Obat Pemerah Bibir Piaohong adalah gel pemerah bibir alami yang sangat berkhasiat menjadikan bibir lebih merah natural, cocok untuk wanita/Pria

Obat Mata Herbal Gleaming Eyes adalah obat mata berbentuk pills dan tetes terbuat dari ekstra ramuan herbal untuk syaraf penglihatan dan jaringan bola mata

Obat Penghilang Jerawat Alami Yofume Acne Removing adalah produk kosmetik yang sangat ampuh membersihkan jerawat secara cepat dan alami,

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Nangen Kapsul Obat Kuat Herbal Yang Berfungsi Mengatasi Ejakulasi Dini Disfungsi Ereksi Dan Problem Seksualitas Pria Lainya.Terbuat Dari Gingseng China.

Maximum Powerful Obat Kuat Pria adalah ramuan herbal alami yang berkhasiat sebagai obat kuat tahan lama,serta mengatasi ejakulasi dini, impotensi dan dll

Meizitang Obat Pelangsing Badan Herbal Merupakan Suplemen Diet Herbal Berbentuk SOFTGEL Yang Sangat Berkhasiat Melangsingkan Tubuh Dengan Cepat Dan Aman,

Obat Pelangsing Badan Herbal Fatloss Jimpness Beauty Penghilang Lemak Seketika Merupakan Obat Pelangsing Badan Yang Aman Dikonsumsi Untuk Pria Maupun Wanita

Pelangsing Badan Herbal Lida Daidaihua Adalah Pelangsing Badan Yang Mengandung Bahan Herbal Yang Sangat Cepat Menurunkan Berat Badan, Aman Dan Cepat .

Obat Perangsang Libido Wanita Blue Wizard Adalah Obat Perangsang Wanita Alami Yang Berasal Dari Jerman, Sangat Manjur Untuk Wanita Kurang Bergairah (Firgid)

The global COVID-19 spread has gone from bad to worse, with over 3.2 million confirmed cases and close to 250,000 deaths, not only has this pandemic claimed innumerable lives, it has also destabilised economies by freezing trade and other economic...

At age 13, Nadeen Matthews Blair, chief executive officer of the NCB Foundation, challenged the tide to prove to a guidance counsellor at her United States-based school, that black girls can overcome all odds to become powerful leaders. Matthews...

The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs.

The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs.

The signing of veteran outfielder Michael Brantley by the Astros came a week after the Winter Meetings and only a few days before Christmas, meaning it didn't exactly garner much attention on national, or even local, news cycles. For the Astros, Brantley was exactly the player they needed at exactly the right price -- two years and $32 million -- and the kind of addition that should fire up the fanbase.

The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs.

The future of health and social care looks certain to be a defining issue in the forthcoming UK general election. Social care has been subject to deep public spending cuts, raising concerns about the sustainability of services in the future. Whoever wins the next election will need to grapple with providing joined up health and social care...

Bobbie Farsides is professor of clinical and biomedical ethics at Brighton and Sussex Medical School. She’s been described as one of the few people that is acceptable to “both sides” of the assisted dying debate. This week she joins us to talk about the way in which the debate on euthanasia has played out in the UK - and hear why she thinks it’s...

Nicole Stott is an engineer, aquanaut and one of the 220 astronauts to have lived and worked on the International Space Station. In a confined space, under huge pressure, with no way out, it's important that teams maintain healthy dynamics, and individuals can manage their stress adequately, and in this podcast Nicole explains a little about...

For the next few months Talk Evidence is going to focus on the new corona virus pandemic. There is an enormous amount of uncertainty about the disease, what the symptoms are, fatality rate, treatment options, things we shouldn't be doing. We're going to try to get away from the headlines and talk about what we need to know - to hopefully give you...

Unlike recent Reds Spring Trainings, much of the drama about who would comprise the rotation was already removed on the first day of camp. That's when manager David Bell revealed the starting five would likely be -- in no particular order -- Sonny Gray, Tanner Roark, Alex Wood, Luis Castillo and Anthony DeSclafani.

The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs.

The next five weeks will see lots of shuffling on Major League rosters. Here are the most intriguing positional battles on each of the 30 MLB clubs.

Members Event

Event participants

Stephen Gethins, Candidate for Fife North East and Shadow Spokesperson for Foreign and Commonwealth Office (2018-19), Scottish National Party (remote)
Dominic Raab, Candidate for Esher & Walton, Foreign Secretary and First Secretary of State (2019), Conservative Party
Emily Thornberry, Candidate for Islington South & Finsbury and Shadow Foreign Secretary (2016-19), Labour Party
Chuka Umunna, Candidate for Cities of London & Westminster and Spokesperson for Foreign and Commonwealth Affairs (2019), Liberal Democrat Party
Chair: Ritula Shah, The World Tonight, BBC Radio 4
 

Invitation Only Research Event

Event participants

Duncan Robinson, Charlemagne Columnist; Brussels Bureau Chief, the Economist
Chair: Pepijn Bergsen, Research Fellow, Europe Programme, Chatham House

As Greece's Aegean islands continue to grapple with migrants arriving on their shores, decisions regarding the needs of newcomers are negotiated in Brussels and Athens, far removed from the situation on the ground. Meanwhile, local communities have had successes in hosting migrants, as this article drawing on observations from the hospitality center and refugee camp on Lesvos explores.

OBJECTIVE

Dysglycemia, in this survey defined as impaired glucose tolerance (IGT) or type 2 diabetes, is common in patients with coronary artery disease (CAD) and associated with an unfavorable prognosis. This European survey investigated dysglycemia screening and risk factor management of patients with CAD in relation to standards of European guidelines for cardiovascular subjects.

The Pentagon announced Tuesday that Sgt. Christopher Wesley Curry died Monday in Iraq in what officials describe as a non-combat-related incident.

Here’s my Q&A column from the WSJ this week — and if you have any questions for me, you can tweet them to @danariely with the hashtag #askariely, post a comment on my Ask Ariely Facebook page, or email them to AskAriely@wsj.com. ___________________________________________________ Dear Dan, People in my office drink a...

Lovely sweet and easy to bake cookies.

A West Virginia official turns up the heat on financial disclosure for the state’s beleaguered schools, and ruffles feathers in the process.

Court-ordered school desegregation has been more successful in the South than in any other region of the country, but researchers have noted a new threat: the growing number of communities that are seceding from larger school districts to form their own.

Court-ordered school desegregation has been more successful in the South than in any other region of the country, but researchers have noted a new threat: the growing number of communities that are seceding from larger school districts to form their own.