strat strataconf: StrataWeek: Wireless body networks bring humans into Internet of Things http://t.co/vRgkRtTTKe Pills & tattoo authenticators & NSA leaks By twitter.com Published On :: Sat, 08 Jun 2013 09:10:16 +0000 strataconf: StrataWeek: Wireless body networks bring humans into Internet of Things http://t.co/vRgkRtTTKe Pills & tattoo authenticators & NSA leaks Full Article
strat strataconf: Can data rescue the forest elephant? Yes, with your help. http://t.co/DZBXKRdUcA Read about Elephant Listening Project & send us your ideas By twitter.com Published On :: Sat, 08 Jun 2013 17:32:07 +0000 strataconf: Can data rescue the forest elephant? Yes, with your help. http://t.co/DZBXKRdUcA Read about Elephant Listening Project & send us your ideas Full Article
strat strataconf: Moving to the open healthcare graph http:// http://t.co/YYTUDN3Vzn Achieving the triple aim in healthcare: better, cheaper, safer #stratarx By twitter.com Published On :: Sat, 08 Jun 2013 21:02:16 +0000 strataconf: Moving to the open healthcare graph http:// http://t.co/YYTUDN3Vzn Achieving the triple aim in healthcare: better, cheaper, safer #stratarx Full Article
strat strataconf: Innovative ways journalists are using data to tell stories http://t.co/y8RVUwHO4G Global open data, scholarships, mapping a civil war & more By twitter.com Published On :: Sun, 09 Jun 2013 11:25:28 +0000 strataconf: Innovative ways journalists are using data to tell stories http://t.co/y8RVUwHO4G Global open data, scholarships, mapping a civil war & more Full Article
strat strataconf: A roundup of healthcare tools used in the field from #hdpalooza http://t.co/0d2x3OlaeC including @MedCPU @SVBiosystems @CHRankings & more By twitter.com Published On :: Sun, 09 Jun 2013 15:11:18 +0000 strataconf: A roundup of healthcare tools used in the field from #hdpalooza http://t.co/0d2x3OlaeC including @MedCPU @SVBiosystems @CHRankings & more Full Article
strat strataconf: Building Big Data applications now easier http://t.co/jaATalPL4P Hadoop analytic engines simplify creation of low-cost, scalable apps By twitter.com Published On :: Mon, 10 Jun 2013 10:55:30 +0000 strataconf: Building Big Data applications now easier http://t.co/jaATalPL4P Hadoop analytic engines simplify creation of low-cost, scalable apps Full Article
strat strataconf: TUE June 11 Best of Strata Webcast: Practical Strategies & Tactics for Moving to Big Data http://t.co/qn4gKSyBvw from #Strataconf + HW2012 By twitter.com Published On :: Mon, 10 Jun 2013 16:11:42 +0000 strataconf: TUE June 11 Best of Strata Webcast: Practical Strategies & Tactics for Moving to Big Data http://t.co/qn4gKSyBvw from #Strataconf + HW2012 Full Article
strat strataconf: Humans as nodes, pills & electronic tattoo password authenticators & hiding data in temporal cloaks http://t.co/vRgkRtTTKe #strataconf By twitter.com Published On :: Mon, 10 Jun 2013 19:21:10 +0000 strataconf: Humans as nodes, pills & electronic tattoo password authenticators & hiding data in temporal cloaks http://t.co/vRgkRtTTKe #strataconf Full Article
strat strataconf: A detailed agenda for #StrataRx 2013 is now posted: workshops, sessions, speakers + more http://t.co/RtaRpQroaN #healthdata By twitter.com Published On :: Mon, 10 Jun 2013 20:57:23 +0000 strataconf: A detailed agenda for #StrataRx 2013 is now posted: workshops, sessions, speakers + more http://t.co/RtaRpQroaN #healthdata Full Article
strat strataconf: Big data vs big reality http://t.co/D751jk0ntb Data itself has no quality. It’s what you do with it that counts By twitter.com Published On :: Mon, 10 Jun 2013 22:41:01 +0000 strataconf: Big data vs big reality http://t.co/D751jk0ntb Data itself has no quality. It’s what you do with it that counts Full Article
strat strataconf: Starts in 1 hour: Best of #Strataconf Webcast - 'Practical Strategies & Tactics for Moving to Big Data'. Watch at http://t.co/qn4gKSyBvw By twitter.com Published On :: Tue, 11 Jun 2013 11:00:53 +0000 strataconf: Starts in 1 hour: Best of #Strataconf Webcast - 'Practical Strategies & Tactics for Moving to Big Data'. Watch at http://t.co/qn4gKSyBvw Full Article
strat strataconf: RT @synergicpartner: We are pleased to announce that we will sponsor next #strataconf in London!!! @strataconf @synergicpartner #BigData ht… By twitter.com Published On :: Tue, 11 Jun 2013 14:07:38 +0000 strataconf: RT @synergicpartner: We are pleased to announce that we will sponsor next #strataconf in London!!! @strataconf @synergicpartner #BigData ht… Full Article
strat strataconf: As infrastructure gets simpler, interesting big data apps can emerge on BDAS, @Cloudera & other Hadoop distributions http://t.co/jaATalPL4P By twitter.com Published On :: Tue, 11 Jun 2013 16:17:47 +0000 strataconf: As infrastructure gets simpler, interesting big data apps can emerge on BDAS, @Cloudera & other Hadoop distributions http://t.co/jaATalPL4P Full Article
strat strataconf: Ways to put the patient first when collecting health data http://t.co/iACckzJjAW @praxagora #stratarx #healthit By twitter.com Published On :: Tue, 11 Jun 2013 17:01:16 +0000 strataconf: Ways to put the patient first when collecting health data http://t.co/iACckzJjAW @praxagora #stratarx #healthit Full Article
strat strataconf: The Future Is Graph Databases http://t.co/BVxOZwtoKS A Conversation with @EmilEifrem, founder of @Neo4J #strataconf By twitter.com Published On :: Tue, 11 Jun 2013 18:25:30 +0000 strataconf: The Future Is Graph Databases http://t.co/BVxOZwtoKS A Conversation with @EmilEifrem, founder of @Neo4J #strataconf Full Article
strat Realtors® Applaud Administration’s Calls to End Conservatorship By feedproxy.google.com Published On :: Wed, 17 Oct 2018 16:59:27 +0000 Realtors® Applaud Administration’s Calls to End... Earlier this week, during an address to the Mortgage Bankers Association 2018 Annual Conference in Washington, D.C, Treasury Department Secretary... By: Wesley Shaw Full Article
strat velocityconf: Call for speakers and registration is open for @webperfdays in Silicon Valley http://t.co/cJKBmynkjI June 22, Google HQ (cc @sfwebperf) By feedproxy.google.com Published On :: Wed, 29 May 2013 15:30:51 +0000 velocityconf: Call for speakers and registration is open for @webperfdays in Silicon Valley http://t.co/cJKBmynkjI June 22, Google HQ (cc @sfwebperf) Full Article
strat Why and How to Effectively Use Webinars in Your Content Strategy By feedproxy.google.com Published On :: Tue, 12 Mar 2019 00:00:00 +0000 You’ve probably attended a webinar or two (or more), but are you using them effectively as part of your overall content plan? In this blog post, we teach you the basics of hosting a webinar and how to use them effectively as you build out your content strategy and grow your business. Full Article
strat Why and How to Effectively Use Webinars in Your Content Strategy By feedproxy.google.com Published On :: Tue, 12 Mar 2019 00:00:00 +0000 You’ve probably attended a webinar or two (or more), but are you using them effectively as part of your overall content plan? In this blog post, we teach you the basics of hosting a webinar and how to use them effectively as you build out your content strategy and grow your business. Full Article
strat AT#22 - Travel to Disneyland (Strategy) By traffic.libsyn.com Published On :: Sat, 24 Dec 2005 19:57:00 +0000 Disneyland (Strategy) Full Article
strat MSNBC’s Brian Williams Chuckles With Dem Strategist as He Gloats, Mocks Trump About Tragic Downturn in Economy: “They were going to lose before this hit. They’re just going to lose worse now” By 100percentfedup.com Published On :: Thu, 07 May 2020 15:29:05 +0000 The following article, MSNBC’s Brian Williams Chuckles With Dem Strategist as He Gloats, Mocks Trump About Tragic Downturn in Economy: “They were going to lose before this hit. They’re just going to lose worse now”, was first published on 100PercentFedUp.com. James Carville spoke out before the coronavirus crisis to say that there is no way Joe Biden has a chance at beating President Trump in the 2020 election. Well, He’s singing a different tune now at the expense of Americans suffering through this horrible pandemic and economic crisis. James Carvill is a Democratic strategist who […] Continue reading: MSNBC’s Brian Williams Chuckles With Dem Strategist as He Gloats, Mocks Trump About Tragic Downturn in Economy: “They were going to lose before this hit. They’re just going to lose worse now” ... Full Article Breaking Featured Left News Politics
strat Modification of a PE/PPE substrate pair reroutes an Esx substrate pair from the mycobacterial ESX-1 type VII secretion system to the ESX-5 system [Molecular Bases of Disease] By feedproxy.google.com Published On :: 2020-05-01T00:06:09-07:00 Bacterial type VII secretion systems secrete a wide range of extracellular proteins that play important roles in bacterial viability and in interactions of pathogenic mycobacteria with their hosts. Mycobacterial type VII secretion systems consist of five subtypes, ESX-1–5, and have four substrate classes, namely, Esx, PE, PPE, and Esp proteins. At least some of these substrates are secreted as heterodimers. Each ESX system mediates the secretion of a specific set of Esx, PE, and PPE proteins, raising the question of how these substrates are recognized in a system-specific fashion. For the PE/PPE heterodimers, it has been shown that they interact with their cognate EspG chaperone and that this chaperone determines the designated secretion pathway. However, both structural and pulldown analyses have suggested that EspG cannot interact with the Esx proteins. Therefore, the determining factor for system specificity of the Esx proteins remains unknown. Here, we investigated the secretion specificity of the ESX-1 substrate pair EsxB_1/EsxA_1 in Mycobacterium marinum. Although this substrate pair was hardly secreted when homologously expressed, it was secreted when co-expressed together with the PE35/PPE68_1 pair, indicating that this pair could stimulate secretion of the EsxB_1/EsxA_1 pair. Surprisingly, co-expression of EsxB_1/EsxA_1 with a modified PE35/PPE68_1 version that carried the EspG5 chaperone-binding domain, previously shown to redirect this substrate pair to the ESX-5 system, also resulted in redirection and co-secretion of the Esx pair via ESX-5. Our results suggest a secretion model in which PE35/PPE68_1 determines the system-specific secretion of EsxB_1/EsxA_1. Full Article
strat Substrate recognition and ATPase activity of the E. coli cysteine/cystine ABC transporter YecSC-FliY [Microbiology] By feedproxy.google.com Published On :: 2020-04-17T00:06:05-07:00 Sulfur is essential for biological processes such as amino acid biogenesis, iron–sulfur cluster formation, and redox homeostasis. To acquire sulfur-containing compounds from the environment, bacteria have evolved high-affinity uptake systems, predominant among which is the ABC transporter family. Theses membrane-embedded enzymes use the energy of ATP hydrolysis for transmembrane transport of a wide range of biomolecules against concentration gradients. Three distinct bacterial ABC import systems of sulfur-containing compounds have been identified, but the molecular details of their transport mechanism remain poorly characterized. Here we provide results from a biochemical analysis of the purified Escherichia coli YecSC-FliY cysteine/cystine import system. We found that the substrate-binding protein FliY binds l-cystine, l-cysteine, and d-cysteine with micromolar affinities. However, binding of the l- and d-enantiomers induced different conformational changes of FliY, where the l- enantiomer–substrate-binding protein complex interacted more efficiently with the YecSC transporter. YecSC had low basal ATPase activity that was moderately stimulated by apo FliY, more strongly by d-cysteine–bound FliY, and maximally by l-cysteine– or l-cystine–bound FliY. However, at high FliY concentrations, YecSC reached maximal ATPase rates independent of the presence or nature of the substrate. These results suggest that FliY exists in a conformational equilibrium between an open, unliganded form that does not bind to the YecSC transporter and closed, unliganded and closed, liganded forms that bind this transporter with variable affinities but equally stimulate its ATPase activity. These findings differ from previous observations for similar ABC transporters, highlighting the extent of mechanistic diversity in this large protein family. Full Article
strat Modification of a PE/PPE substrate pair reroutes an Esx substrate pair from the mycobacterial ESX-1 type VII secretion system to the ESX-5 system [Molecular Bases of Disease] By feedproxy.google.com Published On :: 2020-05-01T00:06:09-07:00 Bacterial type VII secretion systems secrete a wide range of extracellular proteins that play important roles in bacterial viability and in interactions of pathogenic mycobacteria with their hosts. Mycobacterial type VII secretion systems consist of five subtypes, ESX-1–5, and have four substrate classes, namely, Esx, PE, PPE, and Esp proteins. At least some of these substrates are secreted as heterodimers. Each ESX system mediates the secretion of a specific set of Esx, PE, and PPE proteins, raising the question of how these substrates are recognized in a system-specific fashion. For the PE/PPE heterodimers, it has been shown that they interact with their cognate EspG chaperone and that this chaperone determines the designated secretion pathway. However, both structural and pulldown analyses have suggested that EspG cannot interact with the Esx proteins. Therefore, the determining factor for system specificity of the Esx proteins remains unknown. Here, we investigated the secretion specificity of the ESX-1 substrate pair EsxB_1/EsxA_1 in Mycobacterium marinum. Although this substrate pair was hardly secreted when homologously expressed, it was secreted when co-expressed together with the PE35/PPE68_1 pair, indicating that this pair could stimulate secretion of the EsxB_1/EsxA_1 pair. Surprisingly, co-expression of EsxB_1/EsxA_1 with a modified PE35/PPE68_1 version that carried the EspG5 chaperone-binding domain, previously shown to redirect this substrate pair to the ESX-5 system, also resulted in redirection and co-secretion of the Esx pair via ESX-5. Our results suggest a secretion model in which PE35/PPE68_1 determines the system-specific secretion of EsxB_1/EsxA_1. Full Article
strat The Russian challenge demands a more robust Western strategy By feedproxy.google.com Published On :: Fri, 10 Jul 2015 09:23:28 +0000 4 June 2015 20150515RussianChallenge.jpg Photo: AP Photo/Alexander Zemlianichenko It is now clear that President Putin’s ‘new model Russia’ cannot be constructively accommodated into the international system. The war in Ukraine, in part the result of the West's laissez-faire approach to Russia, demonstrates the need for a new Western strategy towards Russia.The Russian Challenge - a major new report by six authors from the Russia and Eurasia Programme at Chatham House - argues that a new strategy must recognise that: The decline of the Russian economy, the costs of confrontation and the rise of China mean that the Putin regime is now facing the most serious challenge of its 15 years in power. The West has neither the wish nor the means to promote regime change in Russia. But Western countries need to consider the possible consequences of a chaotic end to the Putin system. A critical element in the new geo-economic competition between the West and Russia is the extent of Western support for Ukraine, whose reconstruction as an effective sovereign state, capable of standing up for itself, is crucial. This will require much greater resources than have been invested up until now. Russia has rapidly developed its armed forces and information warfare capabilities since the war in Georgia in 2008. The West must invest in defensive strategic communications and media support to counter the Kremlin’s false narratives and restore its conventional deterrent capabilities as a matter of urgency. In particular, NATO needs to demonstrate that the response to ‘ambiguous’ or ‘hybrid’ war will be robust. Sanctions are exerting economic pressure on the Russian leadership and should remain in place until Ukraine’s territorial integrity is properly restored. In particular, it is self-defeating to link the lifting of sanctions solely to implementation of the poorly crafted and inherently fragile Minsk accords. While deterrence and constraint are essential in the short term, the West must also prepare for an eventual change of leadership in Russia. There is a reasonable chance that current pressures will incline a future Russian leadership to want to re-engage with the West.James Nixey, Head of the Russia and Eurasia Programme at Chatham House, said: 'Pursuing these goals and achieving these objectives will ensure that the West is better prepared for any further deterioration in relations with Russia. The events of the last 18 months have demonstrated conclusively that when dealing with Russia, optimism is not a strategy.' Editor's notes Read the report The Russian Challenge from the Russia and Eurasia Programme, Chatham House.Embargoed until Thursday 4 June, 00:01 BST.This report will be launched at an event at Chatham House on Friday 5 June.For all enquiries, please contact the press office. Contacts Press Office +44 (0)20 7957 5739 Email Full Article
strat Transatlantic Strategy Group on the Future of US Global Leadership: Responding to a Revanchist Russia By feedproxy.google.com Published On :: Mon, 08 Sep 2014 13:30:01 +0000 Invitation Only Research Event 12 September 2014 - 8:45am to 5:00pm Chatham House, London Meeting Notepdf | 129.55 KB The situation in Ukraine remains in flux and despite Europe and the US toughening sanctions on Russia, President Putin continues to increase the scope of Russia’s involvement in the conflict between the Ukrainian government and the separatists. It remains unclear how far Putin is willing to go, what his broader regional ambitions are, and what he will do if forced further into a corner by Western actions. In this time of uncertainty and instability it is therefore vital to assess how the transatlantic partners should respond to this increasingly precarious situation. At this all-day event, the group will discuss how US policy towards Russia is changing, what this means for Europe and, subsequently, how Europe should respond. Attencance at this event is by invitation only.The event is part of the Transatlantic Strategy Group on the Future of US Global Leadership run jointly with the German Marshall Fund of the United States. Over the course of a year, this group will come together to discuss how US policy is changing on key issues and the implications for Europe. This project is supported by the Fritz Thyssen Stiftung. Department/project US and the Americas Programme Rory Kinane +44 (0) 20 7314 3650 Email Full Article
strat Transatlantic Strategy Group on the Future of US Global Leadership: Transatlantic Security Policy Towards a Changing Middle East By feedproxy.google.com Published On :: Thu, 05 Mar 2015 11:45:02 +0000 Invitation Only Research Event 6 February 2015 - 8:45am to 4:30pm Residence of the British Ambassador to France, Paris Meeting Summarypdf | 95.65 KB With the Middle East in chaos and the future of many states increasingly uncertain, there is a large amount of attention as to how policy-makers in Europe and the US should respond. In particular, many in Europe are unsure of long-term US policy in light of competing American priorities, budgetary constraints and a public adverse to committing further resources abroad. In this context, it is important that European and American policy-makers understand each other’s positions.At this all-day event, a group of experts will discuss how US policy towards the Middle East is changing, what this means for Europe and, subsequently, how Europe should respond. Attendance at this event is by invitation only.The workshop is held as part of the Transatlantic Strategy Group on the Future of US Global Leadership run jointly with the German Marshall Fund of the United States. Over the course of a year, this group will discuss how US policy is changing on key issues and the implications for Europe. This project is supported by the Fritz Thyssen Foundation, with support for this event provided by the Delegation of Strategic Affairs of the French Ministry of Defence and the British Embassy in Paris. Event attributes External event Department/project US and the Americas Programme Full Article
strat Scholarship and the ship of state: rethinking the Anglo-American strategic decline analogy By feedproxy.google.com Published On :: Thu, 12 Mar 2015 11:44:46 +0000 12 March 2015 , Volume 91, Number 2 Katherine C. Epstein Full Article
strat Implementing the NATO Wales Summit: From Strategy to Action By feedproxy.google.com Published On :: Mon, 16 Mar 2015 11:00:01 +0000 Invitation Only Research Event 26 February 2015 - 1:00pm to 27 February 2015 - 5:00pm German Institute for International and Security Affairs, Berlin, Germany Meeting Summarypdf | 80.56 KB Event participants Xenia Wickett, Project Director, US; Dean, The Queen Elizabeth II Academy for Leadership in International Affairs, Chatham HouseDr Christian Moelling, International Security Division Associate, SWP-Berlin The NATO Summit, held in September 2014 in Newport, Wales, was a waypoint in the larger strategic vision for NATO over the coming decade. As the alliance continues to confront challenges on its eastern and southern frontiers, it must find ways to adapt its strategy for more effective responses, while also staying ahead of emerging threats and risks. The event will bring together senior representatives from a number of the NATO member states, NATO partners and external experts from industry, the media and the think-tank and academic communities, to examine what NATO member states have done thus far to move the deliverables forward, and how to most effectively work together in continuing to do so. Attendance at this event is by invitation only.This is the second of two workshops held in collaboration with SWP-Berlin; the first took place at Chatham House in October 2014. Event attributes External event Department/project US and the Americas Programme, NATO: Charting the Way Forward Full Article
strat Transatlantic Strategy Group on the Future of US Global Leadership: Global Institutions and the Economy of the Future By feedproxy.google.com Published On :: Thu, 01 Oct 2015 13:30:01 +0000 Invitation Only Research Event 10 June 2015 - 8:45am to 4:30pm Bertelsmann Foundation, Berlin, Germany Meeting Summarypdf | 154.74 KB Europe and the United States have dominated global institutions for over 70 years. However, as the emerging markets take up a greater share of the global economy it is becoming increasingly difficult for the transatlantic powers to maintain the current system. This event will examine the changes needed in order to avoid a collapse of the current system.The workshop is held as part of the Transatlantic Strategy Group on the Future of US Global Leadership run jointly with the German Marshall Fund of the United States. Over the course of a year, this group will discuss how US policy is changing on key issues and the implications for Europe. This project is supported by the Fritz Thyssen Foundation, with support for this event provided by the Bertelsmann Foundation. Event attributes External event Department/project US and the Americas Programme US and Americas Programme Email Full Article
strat Realizing TTIP’s Strategic Potential By feedproxy.google.com Published On :: Tue, 12 Jul 2016 15:34:44 +0000 14 July 2016 The strategic case for TTIP is greater – and the stakes higher – now that the UK has decided to leave the EU. But TTIP will create new risks for the West whether it succeeds or fails. Download PDF Gregor Irwin Chief economist, Global Counsel 2016-07-14-ttip.jpg The container ship Osaka Express, operated by Hapag-Lloyd AG, leaves the container terminal at the port in Southampton, UK, on 2 October 2015. Photo: Getty Images. SummaryThe Transatlantic Trade and Investment Partnership (TTIP) – currently being negotiated between the United States and the European Union – represents a bolder and riskier approach to liberalizing trade than traditional trade deals. It is bolder because it aims to cover a wide range of policy issues that are not typically included, and because it aims to be strategic and extraterritorial in its impact. It is riskier because of the difficulty in getting agreement on these issues between the parties concerned, and because the responses of other countries are uncertain.EU and US negotiators have much work to do if a deal is to be reached that realizes TTIP’s full potential. While progress has been made on tariff reduction, considerable ground still needs to be covered on setting standards and regulations affecting trade, which is where TTIP has the most potential to make an impact at a strategic level. Meanwhile, trade scepticism is rising on both sides of the Atlantic, with concerns about the impact of deals like TTIP on jobs and regulatory standards. The negotiation process is also now complicated by the United Kingdom’s decision to leave the EU. Making a clear and credible strategic case for TTIP may be necessary if the negotiations are to continue and be successful.If the United States and the EU are able to agree on the regulations and standards affecting international trade, they will have the scale to define these globally for years to come. International leadership in this area brings commercial advantages and benefits for consumers in the EU and the United States, and it also often is a global public good. However, it sometimes pits EU and US interests against those of other countries, creating scope for conflict over policies in areas such as the rules governing state-owned enterprises.There would be benefits to the United Kingdom, the EU and the United States if the United Kingdom joins TTIP once it is agreed. In the intervening period, it makes sense for the United Kingdom to participate actively in EU decisions regarding TTIP while still a member of the EU, as this could help to smooth an eventual British accession to the partnership.The soft-power benefits from TTIP are potentially substantial, but they would only be maximized if other strategically important countries, such as Turkey, are able to join. Perhaps the clearest sign of TTIP being likely to meet or exceed the highest ambitions for its strategic impact would be if it has an even broader geographical reach, drawing in countries in other regions.The potential security benefits from TTIP are marginal and overhyped. The benefits that unity on trade would bring to transatlantic security are intangible and hard to substantiate. The energy security benefits are likely to be limited, as the volumes of US liquefied natural gas (LNG) exports to the EU are likely to be small. If the EU and the United States are serious about using TTIP to improve security, they should include defence procurement, but this has never been on the agenda.Not only do the differences between the EU and the United States mean that it will be difficult to get a deal, they also constrain how any deal would be implemented and exploited for strategic purposes. If TTIP is to succeed at a strategic level, both sides must be disciplined, consistent and coordinated in using it as the reference point in their negotiations with other countries. The United States is more capable of acting strategically than the EU, in part because of the difficulties for EU member states in coalescing around a shared set of strategic objectives. Until the EU is able to do this, the United States is likely to have much more influence over the strategic direction of TTIP. If the EU wants to bridge this gap, then the European Council should start by reaching a political agreement on its objectives and priorities for bringing other countries into TTIP.There are also strategic risks from TTIP. One is that by emphasizing the values that are reflected in international rules, the EU and the United States could make it harder for other countries to accept these rules, or make TTIP seem like an attempt to reassert the old world order. Thus, instead of having a magnetic effect, TTIP could create a rift with emerging countries, with some choosing to maintain a distance for political reasons.The bigger risk for TTIP, however, is failure. If negotiations break down or a deal is reached that falls short of the ambition set for it, this would send a damaging signal to the rest of the world about the inability of the EU and the United States to work together. The damage would be all the greater if the process was acrimonious, or if it exposed US indifference to Europe or latent anti-Americanism in the EU. The United Kingdom’s decision to leave the EU raises the stakes, particularly for the latter, as it is a blow to the international credibility of the EU. Agreeing TTIP would help to offset this blow, while failure would amplify it. Department/project US and the Americas Programme, US Geoeconomic Trends and Challenges Full Article
strat A Transatlantic Strategy for 2020: The Political Dimension By feedproxy.google.com Published On :: Wed, 05 Apr 2017 12:04:00 +0000 Invitation Only Research Event 2 May 2017 - 12:00pm to 1:15pm Chatham House, London Event participants Dr Robin Niblett CMG, Director, Chatham HouseChair: Xenia Wickett, Head, US and the Americas Programme and Dean, Queen Elizabeth II Academy for Leadership in International Affairs, Chatham House The transatlantic partnership has provided the backbone to the liberal international order ever since the end of the Second World War. The tumultuous political events of 2016 denote a brutal rupture from the dominant global position of the transatlantic partnership and threaten to undermine the partnership itself. Only by understanding that the current problems facing the transatlantic relationship have deep structural roots will it be possible to find ways to prevent further erosion, sustain the benefits of the existing partnership and build opportunities for transatlantic cooperation in the future. Ahead of the publication of his new paper, Robin Niblett, will join us to share his thoughts on the challenges, opportunities and potential strategies towards securing the future of the transatlantic relationship. Attendance at this event is by invitation only. Event attributes Chatham House Rule Department/project US and the Americas Programme Courtney Rice Senior Programme Manager, US and the Americas Programme (0)20 7389 3298 Email Full Article
strat In Vivo Identification of Human Small Ubiquitin-like Modifier Polymerization Sites by High Accuracy Mass Spectrometry and an in Vitro to in Vivo Strategy By feedproxy.google.com Published On :: 2008-01-01 Ivan MaticJan 1, 2008; 7:132-144Research Full Article
strat Evolution, expression, and substrate specificities of aldehyde oxidase enzymes in eukaryotes [Enzymology] By feedproxy.google.com Published On :: 2020-04-17T00:06:05-07:00 Aldehyde oxidases (AOXs) are a small group of enzymes belonging to the larger family of molybdo-flavoenzymes, along with the well-characterized xanthine oxidoreductase. The two major types of reactions that are catalyzed by AOXs are the hydroxylation of heterocycles and the oxidation of aldehydes to their corresponding carboxylic acids. Different animal species have different complements of AOX genes. The two extremes are represented in humans and rodents; whereas the human genome contains a single active gene (AOX1), those of rodents, such as mice, are endowed with four genes (Aox1-4), clustering on the same chromosome, each encoding a functionally distinct AOX enzyme. It still remains enigmatic why some species have numerous AOX enzymes, whereas others harbor only one functional enzyme. At present, little is known about the physiological relevance of AOX enzymes in humans and their additional forms in other mammals. These enzymes are expressed in the liver and play an important role in the metabolisms of drugs and other xenobiotics. In this review, we discuss the expression, tissue-specific roles, and substrate specificities of the different mammalian AOX enzymes and highlight insights into their physiological roles. Full Article
strat Roles of active-site residues in catalysis, substrate binding, cooperativity, and the reaction mechanism of the quinoprotein glycine oxidase [Enzymology] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 The quinoprotein glycine oxidase from the marine bacterium Pseudoalteromonas luteoviolacea (PlGoxA) uses a protein-derived cysteine tryptophylquinone (CTQ) cofactor to catalyze conversion of glycine to glyoxylate and ammonia. This homotetrameric enzyme exhibits strong cooperativity toward glycine binding. It is a good model for studying enzyme kinetics and cooperativity, specifically for being able to separate those aspects of protein function through directed mutagenesis. Variant proteins were generated with mutations in four active-site residues, Phe-316, His-583, Tyr-766, and His-767. Structures for glycine-soaked crystals were obtained for each. Different mutations had differential effects on kcat and K0.5 for catalysis, K0.5 for substrate binding, and the Hill coefficients describing the steady-state kinetics or substrate binding. Phe-316 and Tyr-766 variants retained catalytic activity, albeit with altered kinetics and cooperativity. Substitutions of His-583 revealed that it is essential for glycine binding, and the structure of H583C PlGoxA had no active-site glycine present in glycine-soaked crystals. The structure of H767A PlGoxA revealed a previously undetected reaction intermediate, a carbinolamine product-reduced CTQ adduct, and exhibited only negligible activity. The results of these experiments, as well as those with the native enzyme and previous variants, enabled construction of a detailed mechanism for the reductive half-reaction of glycine oxidation. This proposed mechanism includes three discrete reaction intermediates that are covalently bound to CTQ during the reaction, two of which have now been structurally characterized by X-ray crystallography. Full Article
strat Evasive balancing: India's unviable Indo-Pacific strategy By feedproxy.google.com Published On :: Wed, 08 Jan 2020 11:37:22 +0000 8 January 2020 , Volume 96, Number 1 Read online Rajesh Rajagopalan India has adopted the Indo-Pacific concept with uncharacteristic speed. This article examines India's Indo-Pacific strategy, which evolved out of its earlier ‘Look East’ and ‘Act East’ policies but is much more focused on strategic concerns than on trade or connectivity. As such, the strategy is subset of its China policy, and includes contradictory elements of balancing China by building partnerships with the United States as well as with regional powers, while simultaneously pursuing a reassurance strategy to convince Beijing that India is not really balancing China. The combination of these contradictory elements is characterized as evasive balancing, which is a more useful concept than either pure balancing or hedging for understanding the policies of India and of many other countries in the region that are trying to manage China's rise. However, reassurance strategies rarely work and the combination of balancing and reassurance is even less likely to be viable. Full Article
strat Is Australia's Indo-Pacific strategy an illusion? By feedproxy.google.com Published On :: Wed, 08 Jan 2020 11:39:40 +0000 8 January 2020 , Volume 96, Number 1 Read online Brendan Taylor Australia has been among the most prominent advocates of the increasingly popular Indo-Pacific concept. This article argues that Canberra's enthusiasm for the concept stems from its appeal to the two dominant traditions of Australian foreign policy—a ‘dependent ally’ tradition and a ‘middle power’ approach. While these two traditions are typically seen as being in tension, the Indo-Pacific concept provides a rare point of convergence between them. The article begins by outlining the appeal of the Indo-Pacific concept to each of these traditions. Using a case-study of recent Australian policy toward the South China Sea disputes, however, the article then demonstrates that Australia has in practice implemented its stated Indo-Pacific strategy far less consistently than its very vocal support would appear to suggest. This disjuncture is attributed to the growing influence of a third, generally understudied, ‘pragmatic’ Australian foreign policy tradition. Because Australia has been such a prominent champion of the Indo-Pacific concept, the article concludes that this divergence between the rhetoric and the reality of Australia's Indo-Pacific strategy threatens to have a negative impact on the concept's broader international appeal and sustainability, particularly among Australia's south-east Asian neighbours. Full Article
strat Consigned to hedge: south-east Asia and America's ‘free and open Indo-Pacific’ strategy By feedproxy.google.com Published On :: Wed, 08 Jan 2020 11:47:11 +0000 8 January 2020 , Volume 96, Number 1 Read online See Seng Tan This article assesses how south-east Asian countries and the Association of Southeast Asian Nations (ASEAN) have responded to the ‘free and open Indo-Pacific’ (FOIP) strategies promoted by the United States and the other countries in the Quadrilateral Security Dialogue (the ‘Quad’: US, Japan, Australia and India). Their nuanced ripostes imply a persistent commitment to hedging and shifting limited alignments in the face of growing great rivalry and the lack of a clear FOIP vision among Quad members. In the face of external pressure to take sides, the ASEAN states are likely to keep hedging through working selectively with China and the United States. Given the United States' apparent preference to balance China and Trump's disregard for multilateralism, ASEAN's ability to maintain its centrality in the evolving regional architecture is in doubt—despite the Quad countries' (belated) accommodation of ASEAN in their FOIP strategies. However, the success of the US strategy depends on Washington's ability to build and sustain the requisite coalition to balance Beijing. ASEAN has undertaken efforts to enhance bilateral security collaboration with China and the United States respectively. In doing so, ASEAN is arguably seeking to informally redefine its centrality in an era of Great Power discord and its ramifications for multilateralism. Full Article
strat Understanding the dynamics of the Indo-Pacific: US–China strategic competition, regional actors, and beyond By feedproxy.google.com Published On :: Wed, 08 Jan 2020 21:20:01 +0000 6 November 2019 , Volume 96, Number 1 The first issue of International Affairs in 2020 explores the geopolitics of the 'Indo-Pacific' region. Read online Kai He and Mingjiang Li As a geographical concept, ‘Indo-Pacific’ has existed for decades. As a political and strategic concept, it has since 2010 gradually become established in the foreign policy lexicon of some countries, especially Australia, India, Japan and the United States. However, China seems to be reluctant to identify itself as part of the Indo-Pacific; Chinese leaders believe that the US-led Indo-Pacific strategy aims to contain China's rise. While the battle between the two geographical concepts ‘Indo-Pacific’ and ‘Asia–Pacific’ may be fairly easily settled in the future, US–China strategic competition has just begun. Will the Indo-Pacific become a battlefield for US–China rivalry? How will China cope with the US ‘free and open Indo-Pacific’ (FOIP) strategy? How will other regional actors respond to the US–China strategic competition in the Indo-Pacific? What are the strategic implications of the ‘Indo-Pacific’ concept for regional order transformation? How will the Indo-Pacific be institutionalized, economically, politically and strategically? This article introduces the January 2020 special issue of International Affairs, which aims to address those questions, using both country-specific and regional perspectives. Seven articles focus on the policy responses of major players (Australia, China, India, Indonesia, Japan and ASEAN) to the US FOIP strategy and related US–China rivalry in the region. A further three articles examine the profound implications of Indo-Pacific dynamics for regional institution-building and for geopolitical and geo-economic architecture. Full Article
strat Civil society perspectives on sexual violence in conflict: patriarchy and war strategy in Colombia By feedproxy.google.com Published On :: Wed, 04 Mar 2020 13:31:21 +0000 4 March 2020 , Volume 96, Number 2 Anne-Kathrin Kreft Read Online In international policy circles, conflict-related sexual violence (CRSV) is commonly viewed as a weapon of war, a framing that researchers have criticized as overly simplistic. Feminist scholars in particular caution that the ‘weapon of war’ framing decontextualizes sexual violence in conflict from the structural factors of gender inequality that underpin its perpetration. In light of these tensions, how do politically relevant local actors perceive the nature and the origins of conflict-related sexual violence? Civil society organizations often actively confront conflict-related sexual violence on the ground. A better understanding of how their perceptions of this violence align or clash with the globally dominant ‘weapon of war’ narratives therefore has important policy implications. Interviews with representatives of Colombian women's organizations and victims' associations reveal that these civil society activists predominantly view conflict-related sexual violence as the result of patriarchal structures. The mobilized women perceive sexual violence as a very gendered violence that exists on a continuum extending through peace, the everyday and war, and which the presence of arms exacerbates. Strategic sexual violence, too, is understood to ultimately have its basis in patriarchal structures. The findings expose a disconnect between the globally dominant ‘weapon of war’ understanding that is decontextualized from structural factors and a local approach to CRSV that establishes clear linkages to societal gender inequality. Full Article
strat Year Two of the Abe Administration: Prospects for the Future of Japanese Foreign Policy and UK-Japan Relations By feedproxy.google.com Published On :: Fri, 24 Jan 2014 15:29:13 +0000 Research Event 11 February 2014 - 3:00pm to 5:00pm Chatham House, London Meeting Summary: Year Two of the Abe Administration: Prospects for the Future of Japanese Foreign Policypdf | 185.89 KB Event participants Kiichi Fujiwara, Professor of International Politics, Graduate School for Law and Politics, Tokyo UniversityYuichi Hosoya, Professor of International Politics, Faculty of Law, Keio University Akiko Yamanaka, Senior Visiting Research Fellow, Churchill College, University of Cambridge; Vice Minister for Foreign Affairs of Japan (2005-2006)Chair: John Swenson-Wright, Senior Consulting Fellow, Asia Programme, Chatham House As Prime Minster Abe enters his second year in office, the speakers will consider future prospects for Japanese foreign policy and UK-Japan relations. This event is funded by the Nippon Foundation. It is held in partnership with the Nippon Foundation and the Great Britain Sasakawa Foundation.THIS EVENT IS NOW FULL AND REGISTRATION HAS CLOSED. Department/project UK-Japan Global Seminar Series Full Article
strat US–China Strategic Competition: The Quest for Global Technological Leadership By feedproxy.google.com Published On :: Wed, 06 Nov 2019 18:26:46 +0000 7 November 2019 The current dispute between the US and China goes far beyond trade tariffs and tit-for-tat reprisals: the underlying driver is a race for global technological supremacy. This paper examines the risks of greater strategic competition as well as potential solutions for mitigating the impacts of the US–China economic confrontation. Read online Download PDF Marianne Schneider-Petsinger Senior Research Fellow, US and the Americas Programme @mpetsinger Dr Jue Wang Associate Fellow, Asia-Pacific Programme (based in Holland) LinkedIn Dr Yu Jie Senior Research Fellow on China, Asia-Pacific Programme @yu_jiec LinkedIn James Crabtree Associate Fellow, Asia-Pacific Programme @jamescrabtree LinkedIn Examining the US-China Trade Competition Video: Marianne Schneider-Petsinger and Dr Yu Jie discuss key themes from the research paperSummaryThe underlying driver of the ongoing US–China trade war is a race for global technological dominance. President Trump has raised a number of issues regarding trade with China – including the US’s trade deficit with China and the naming of China as a currency manipulator. But at the heart of the ongoing tariff escalation are China’s policies and practices regarding forced technology transfer, intellectual property theft and non-market distortions.As China’s international influence has expanded it has always been unlikely that Beijing would continue to accept existing global standards and institutions established and widely practised by developed countries based on ‘the Washington Consensus’.China’s desire to be an alternative champion of technology standard-setting remains unfulfilled. Its ample innovation talent is a solid foundation in its quest for global technology supremacy but tightening controls over personal freedoms could undermine it and deter potential global partners.It is unclear if Chinese government interventions will achieve the technological self-sufficiency Beijing has long desired. China’s approach to macroeconomic management diverges significantly from that of the US and other real market economies, particularly in its policy towards nurturing innovation.Chinese actors are engaged in the globalization of technological innovation through exports and imports of high-tech goods and services; cross-border investments in technology companies and research and development (R&D) activities; cross-border R&D collaboration; and international techno-scientific research collaboration.While the Chinese state pushes domestic companies and research institutes to engage in the globalization of technological innovation, its interventions in the high-tech sector have caused uneasiness in the West.The current US response to its competition with China for technological supremacy, which leans towards decoupling, is unlikely to prove successful. The US has better chances of success if it focuses on America’s own competitiveness, works on common approaches to technology policy with like-minded partners around the globe and strengthens the international trading system.A technically sound screening mechanism of foreign investment can prevent normal cross-border collaboration in technological innovation from being misused by geopolitical rival superpowers. Department/project Asia-Pacific Programme, Trade, Investment and Economics, US and the Americas Programme, US Geoeconomic Trends and Challenges Full Article
strat US 2020: America’s National Security Strategy and Middle East Policy By feedproxy.google.com Published On :: Tue, 28 Jan 2020 14:00:02 +0000 Invitation Only Research Event 10 February 2020 - 10:30am to 11:30am Chatham House | 10 St James's Square | London | SW1Y 4LE Event participants Dr Kori Schake, Resident Scholar and Director of Foreign and Defense Policy Studies, American Enterprise Institute Chair: Dr Leslie Vinjamuri, Director, US and Americas Programme In the run-up to the 2016 US presidential election, then-candidate Donald Trump made a series of campaign promises concerning US foreign policy towards the Middle East. Since assuming office, President Trump has withdrawn the US from the Joint Comprehensive Plan of Action, withdrawn troops from Syria, relocated the US embassy in Israel to Jerusalem and orchestrated the strike against ISIS leader Abu Bakr al-Baghdadi.Against a backdrop of Trump's inclination towards withdrawing from the region, countries across the Middle East are being rocked by protests, Turkey’s purchase of Russia’s S-400 missile has threatened to undermine cohesion within NATO and the much hoped for ceasefire in Libya between UN-backed government leader, Fayez al-Sarraj, and opposition leader, Khalifa Haftar, failed to materialize.In light of the upcoming US elections in November 2020, the future of US national security policy promises to be a prominent issue for the next administration. In this vein, the US and Americas Programme at Chatham House plans a yearlong focus on the pivotal US 2020 elections.At this event, Dr Kori Schake, director of foreign and defense policy studies at the American Enterprise Institute will discuss the future of US foreign policy towards the Middle East. How have domestic and party politics in the US – and the unfolding presidential campaign – shaped recent policy decisions by the Trump administration? Should we expect policy objectives in the Middle East to remain consistent or shift under a second Trump term? And what direction could US foreign policy towards the region take under a Democratic administration?Attendance at this event is by invitation only. Event attributes Chatham House Rule Department/project US and the Americas Programme US and Americas Programme Email Full Article
strat Civil society perspectives on sexual violence in conflict: patriarchy and war strategy in Colombia By feedproxy.google.com Published On :: Wed, 04 Mar 2020 13:31:21 +0000 4 March 2020 , Volume 96, Number 2 Anne-Kathrin Kreft Read Online In international policy circles, conflict-related sexual violence (CRSV) is commonly viewed as a weapon of war, a framing that researchers have criticized as overly simplistic. Feminist scholars in particular caution that the ‘weapon of war’ framing decontextualizes sexual violence in conflict from the structural factors of gender inequality that underpin its perpetration. In light of these tensions, how do politically relevant local actors perceive the nature and the origins of conflict-related sexual violence? Civil society organizations often actively confront conflict-related sexual violence on the ground. A better understanding of how their perceptions of this violence align or clash with the globally dominant ‘weapon of war’ narratives therefore has important policy implications. Interviews with representatives of Colombian women's organizations and victims' associations reveal that these civil society activists predominantly view conflict-related sexual violence as the result of patriarchal structures. The mobilized women perceive sexual violence as a very gendered violence that exists on a continuum extending through peace, the everyday and war, and which the presence of arms exacerbates. Strategic sexual violence, too, is understood to ultimately have its basis in patriarchal structures. The findings expose a disconnect between the globally dominant ‘weapon of war’ understanding that is decontextualized from structural factors and a local approach to CRSV that establishes clear linkages to societal gender inequality. Full Article
strat Somaliland's Regional Priorities and Strategic Partnerships By feedproxy.google.com Published On :: Thu, 19 Apr 2018 00:00:00 +0100 Full Article
strat Restraining Great Powers: Soft Balancing Strategies Reconsidered By feedproxy.google.com Published On :: Tue, 15 Jan 2019 00:00:00 +0000 Full Article
strat The 2019 Arab Youth Survey: Pragmatism, Frustration and Optimism By feedproxy.google.com Published On :: Mon, 23 Sep 2019 00:00:00 +0100 Full Article
strat An Improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) Strategy for Precise Quantitative Single-cell Proteomics [Research] By feedproxy.google.com Published On :: 2020-05-01T00:05:26-07:00 Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective processing of single cells. In particular, the concept of using boosting/carrier channels in isobaric labeling to increase the sensitivity in MS detection has also been increasingly used for quantitative proteomic analysis of small-sized samples including single cells. However, the full potential of such boosting/carrier approaches has not been significantly explored, nor has the resulting quantitation quality been carefully evaluated. Herein, we have further evaluated and optimized our recent boosting to amplify signal with isobaric labeling (BASIL) approach, originally developed for quantifying phosphorylation in small number of cells, for highly effective analysis of proteins in single cells. This improved BASIL (iBASIL) approach enables reliable quantitative single-cell proteomics analysis with greater proteome coverage by carefully controlling the boosting-to-sample ratio (e.g. in general <100x) and optimizing MS automatic gain control (AGC) and ion injection time settings in MS/MS analysis (e.g. 5E5 and 300 ms, respectively, which is significantly higher than that used in typical bulk analysis). By coupling with a nanodroplet-based single cell preparation (nanoPOTS) platform, iBASIL enabled identification of ~2500 proteins and precise quantification of ~1500 proteins in the analysis of 104 FACS-isolated single cells, with the resulting protein profiles robustly clustering the cells from three different acute myeloid leukemia cell lines. This study highlights the importance of carefully evaluating and optimizing the boosting ratios and MS data acquisition conditions for achieving robust, comprehensive proteomic analysis of single cells. Full Article
strat The Indo-Pacific: Geostrategic Perspectives to 2024 - Workshop 3 By feedproxy.google.com Published On :: Mon, 14 Oct 2019 09:10:01 +0000 Invitation Only Research Event 17 October 2019 - 9:30am to 2:00pm Institut Francais des Relations Internationales, 27 rue de la Procession, 75740 Paris Cedex 15, France This closed-door roundtable explores possible strategic shifts in the Indo-Pacific between now and 2024. Focusing on trade security, climate change disruptions and security cooperation, it aims to enhance the understanding of the regional goals of, and strategic relationships between, the key countries active in the region.The workshop is part of a larger project funded by the Strategic Policy Division of the Australian Department of Defence. The project includes workshops in the United States, the United Kingdom, France, Japan, India and the Pacific Islands (Tonga).Attendance at this event is by invitation only. Department/project Asia-Pacific Programme, Geopolitics and Governance, Sustainable and Inclusive Growth, Trade, Investment and Economics, Energy, Environment and Resources Programme, Geostrategic Outlook for the Indo-Pacific 2019-2024 Anna Aberg Research Analyst, Energy, Environment and Resources Programme 020 7314 3629 Email Full Article
strat The Indo-Pacific: Geostrategic Outlook to 2024 - Workshop 4 By feedproxy.google.com Published On :: Thu, 09 Jan 2020 11:15:01 +0000 Invitation Only Research Event 26 November 2019 - 9:30am to 12:00pm Gateway House, Stevens Street, Colaba This closed-door roundtable explores possible strategic shifts in the Indo-Pacific between now and 2024.Focusing on trade security, climate change disruptions and security cooperation, it aims to enhance the understanding of the regional goals of, and strategic relationships between, the key countries active in the region.The workshop is part of a larger project funded by the Strategic Policy Division of the Australian Department of Defence.The project includes workshops in the United States, the United Kingdom, France, Japan, India and the Pacific Islands (Tonga). Department/project Asia-Pacific Programme, Geopolitics and Governance, Sustainable and Inclusive Growth, Trade, Investment and Economics, Energy, Environment and Resources Programme Anna Aberg Research Analyst, Energy, Environment and Resources Programme 020 7314 3629 Email Full Article