Apesar de serem menos afetadas pelo coronavírus, crianças são as que podem sofrer maiores efeitos sobre sua saúde no longo prazo, explica pesquisador americano Philip Fisher.

Os ‘lazarettos’ de Dubrovnik são a memória da luta da cidade no combate a doenças infecciosas séculos atrás.

Um dos países de maior sucesso na luta contra a covid-19, a Nova Zelândia permitiu que seus cidadãos expandissem, de forma limitada, o círculo de contatos; entenda como essa proposta funciona e por que está sendo avaliada por vários governos.

Former South Bend, Indiana, mayor and former Democratic hopeful for president Pete Buttigieg responded to the shooting of Dreasjon Reed.

The financial disruptions from the coronavirus pandemic are growing. April brings new challenges for renters, homeowners and small-business owners.

Sue Tameling was in her role as Greenwood Christian AD for less than two years, but she left an impression as a mentor, teacher and a friend.

It lasts 12 weeks and is watched by millions of people on TV. We report on the ultimate job interview.

Wandering around the internet, I was searching for 2 different things at the same time: a hotel for a romantic weekend getaway (with our lovely dog!) and a new apartment to rent. I suddenly asked myself: “Why not renting a vacation home instead of booking the usual same type of hotels (those few hotels that [...]

Former South Bend, Indiana, mayor and former Democratic hopeful for president Pete Buttigieg responded to the shooting of Dreasjon Reed.

This Teacher Appreciation Week, IndyStar asked readers to help recognize some of the amazing teachers going above and beyond during these times.

Former Arsenal and England striker Ian Wright says women's football deserves more support and backing.

Indianapolis, Fishers and the state on Thursday each announced plans which, combined, should result in greater testing capacity for Central Indiana.

A deceased dad in the new "Onward" echoes some of Pixar's most moving moments involving absent relatives.

The “Saved by the Bell” star later apologized for stirring controversy in a resurfaced interview with conservative Candace Owens.

Review of 'The Case for Nationalism: How It Made Us Powerful, United, and Free' by Rich Lowry

REALTORS® Urge Deduction for Rental Income

BitTorrent’s new Project Chrysalis client now makes it possible to share files of unlimited size with your friends even when you’re not online. The company is caching files and using a revamped UI to make the facilitation of BitTorrent downloads much easier than before. Continue reading on NewTeeVee.

BitTorrent founder Bram Cohen and his company are moving forward with its P2P live streaming project, expanding field trials and courting indie bands to stress test Cohen’s algorithms. However, it could still take months before BitTorrent Live is ready for prime time. Continue reading on NewTeeVee.

11 Million Struggling to Pay Mortgage Payments and Rent

The Delivery Man Synopsis The Delivery Man is a comedy television series produced by ITV that premiered in April of 2015. The Delivery Man follows the story of Matthew (played by Darren Boyd – Spy), a police officer who decides to switch careers in his mid-thirties and becomes a midwife. The Delivery Man is currently […]

Other Space Synopsis Other Space is a Science Fiction comedy television series produced by Yahoo that premiered in April of 2015. Created by Paul Feig (Freaks and Geeks) Other Space is set in the early 22nd century and follows the story of the inexperienced crew aboard UMP Cruiser on a routine space exploratory mission. In […]

Peter Kay’s Car Share Synopsis Peter Kay’s Car Share is a situation comedy television series produced by BBC One that premiered in April of 2015. Peter Kay’s Car Share follows the story of John (played by Peter Kay), an assistant manager in a supermarket and his co-worker and promotions rep Kayleigh (played by Sian Gibson). […]

Meet the Smiths Synopsis Meet the Smiths is a scripted reality television series produced by TBS that premiered in April of 2015. Meet the Smiths follows the daily lives of Kenny Smith, an NBA analyst for TNT and his wife Gwendolyn Osborne-Smith, a model for The Price is Right. The couple has five children together […]

Arranged Synopsis Arranged is a reality television series produced by the FYI Network that premiered in April of 2015. Arranged follows the story of the three American couples who all come from different backgrounds, but their cultural traditions allow for arranged marriages. The series chronicles their lives in the lead up to the wedding and […]

Wayward Pines Synopsis Wayward Pines is a mystery thriller television series produced by Fox Network and set to premiere in May of 2015. Based on the book Pines written by Blake Crouch and produced by M. Night Shyamalan Wayward Pines follows the story of an FBI agent Ethan Bruke (played by Matt Dillon), who travels […]

Miles from Tomorrowland Synopsis Miles from Tomorrowland is an animated television series produced by the Disney Channel that premiered in February of 2015. Miles from Tomorrowland is an American animated TV series about the adventures of an inquisitive, if somewhat reckless boy named Miles who travels the universe with his family. Miles from Tomorrowland is […]

The Protectors (1972) Synopsis The Protectors (1972) was an action thriller television series produced by ITV that premiered in September of 1972. The Protectors (1972) was a British action thriller series created by Gerry Anderson (Thunderbirds) about an international group of rich troubleshooters. Contessa di Contini (played by Nyree Dawn Porter), Paul Buchet (played by […]

Wormwood Synopsis Wormwood was a children’s television series produced by Network Ten and ABC1 that premiered in October of 2007. Made as a joint Canadian-Australian production, Wormwood was a TV series about a quirky town where a group of kids investigated strange events. The series was based on the stories written by Paul Jennings. Wormwood […]

A Sharp Intake Of Breath Synopsis A Sharp Intake Of Breath was a situation comedy television series produced by ITV that premiered in February of 1978. Created by Ronnie Taylor A Sharp Intake Of Breath followed the story of Peter Barnes (played by David Jason – Only Fools And Horses), an ordinary man who made […]

The following article, Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative, was first published on 100PercentFedUp.com.

TDS or Trump Derangement Syndrome brings out the worst in a lot of people. One young Texan girl’s story of her intolerant parents who refuse to attend her wedding because she’s marrying a conservative, however, is a new low. Lawyer and conservative civil rights activist, Rogan O’Handley tweeted a Tik Tok video of a Texan […]

Continue reading: Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative ...

Cone photoreceptors in the retina enable vision over a wide range of light intensities. However, the processes enabling cone vision in bright light (i.e. photopic vision) are not adequately understood. Chromophore regeneration of cone photopigments may require the retinal pigment epithelium (RPE) and/or retinal Müller glia. In the RPE, isomerization of all-trans-retinyl esters to 11-cis-retinol is mediated by the retinoid isomerohydrolase Rpe65. A putative alternative retinoid isomerase, dihydroceramide desaturase-1 (DES1), is expressed in RPE and Müller cells. The retinol-isomerase activities of Rpe65 and Des1 are inhibited by emixustat and fenretinide, respectively. Here, we tested the effects of these visual cycle inhibitors on immediate, early, and late phases of cone photopic vision. In zebrafish larvae raised under cyclic light conditions, fenretinide impaired late cone photopic vision, while the emixustat-treated zebrafish unexpectedly had normal vision. In contrast, emixustat-treated larvae raised under extensive dark-adaptation displayed significantly attenuated immediate photopic vision concomitant with significantly reduced 11-cis-retinaldehyde (11cRAL). Following 30 min of light, early photopic vision was recovered, despite 11cRAL levels remaining significantly reduced. Defects in immediate cone photopic vision were rescued in emixustat- or fenretinide-treated larvae following exogenous 9-cis-retinaldehyde supplementation. Genetic knockout of Des1 (degs1) or retinaldehyde-binding protein 1b (rlbp1b) did not eliminate photopic vision in zebrafish. Our findings define molecular and temporal requirements of the nonphotopic or photopic visual cycles for mediating vision in bright light.

Nucleoside analogues are a valuable experimental tool. Incorporation of these molecules into newly synthesized DNA (i.e. pulse-labeling) is used to monitor cell proliferation or to isolate nascent DNA. Some of the most common nucleoside analogues used for pulse-labeling of DNA in cells are the deoxypyrimidine analogues 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyl-2'-deoxycytidine (EdC). Click chemistry enables conjugation of an azide molecule tagged with a fluorescent dye or biotin to the alkyne of the analog, which can then be used to detect incorporation of EdU and EdC into DNA. The use of EdC is often recommended because of the potential cytotoxicity associated with EdU during longer incubations. Here, by comparing the relative incorporation efficiencies of EdU and EdC during short 30-min pulses, we demonstrate significantly lower incorporation of EdC than of EdU in noninfected human fibroblast cells or in cells infected with either human cytomegalovirus or Kaposi's sarcoma-associated herpesvirus. Interestingly, cells infected with herpes simplex virus type-1 (HSV-1) incorporated EdC and EdU at similar levels during short pulses. Of note, exogenous expression of HSV-1 thymidine kinase increased the incorporation efficiency of EdC. These results highlight the limitations when using substituted pyrimidine analogues in pulse-labeling and suggest that EdU is the preferable nucleoside analogue for short pulse-labeling experiments, resulting in increased recovery and sensitivity for downstream applications. This is an important discovery that may help to better characterize the biochemical properties of different nucleoside analogues with a given kinase, ultimately leading to significant differences in labeling efficiency of nascent DNA.

The arrangement of functionally-related genes in operons is a fundamental element of how genetic information is organized in prokaryotes. This organization ensures coordinated gene expression by co-transcription. Often, however, alternative genetic responses to specific stress conditions demand the discoordination of operon expression. During cold temperature stress, accumulation of the gene encoding the sole Asp–Glu–Ala–Asp (DEAD)-box RNA helicase in Synechocystis sp. PCC 6803, crhR (slr0083), increases 15-fold. Here, we show that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/miaB (slr0082) gene, followed by rapid processing of the operon transcript into two monocistronic mRNAs. This cleavage event is required for and results in destabilization of the rimO transcript. Results from secondary structure modeling and analysis of RNase E cleavage of the rimO–crhR transcript in vitro suggested that CrhR plays a role in enhancing the rate of the processing in an auto-regulatory manner. Moreover, two putative small RNAs are generated from additional processing, degradation, or both of the rimO transcript. These results suggest a role for the bacterial RNA helicase CrhR in RNase E-dependent mRNA processing in Synechocystis and expand the known range of organisms possessing small RNAs derived from processing of mRNA transcripts.

JM Dietschy
Oct 1, 1993; 34:1637-1659
Reviews

DL Brasaemle
Nov 1, 1997; 38:2249-2263
Articles