In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

John Quirke stayed in the job for 25 years — with a front row seat to Nunavut's politics as he managed the operations of the assembly and acted as the principal adviser for MLAs on parliamentary procedure among other things. 

The entrepreneur and financial adviser defeated Adam Bassett for the party nomination in Egmont after two days of voting last week. 

CBC's Dalson Chen talks to Bob Reaume about a break in that resulted in an estimated $40,000 in merchandise taken just before the busy holiday season.

The OM team in Bar, Montenegro, do a creative programme in over 15 kindergartens, explaining to children how important it is to regularly brush their teeth.

367.382 mph in a Two-Wheeled Missile

Software helps engineers choose the most promising designs for slicers and sanitation equipment by comparing and contrasting features and performance in virtual design environment

Icebreaker would dispense desperately needed medical care to citizens in polar region countries

SOLIDWORKS and COSMOS analysis software help legendary Italian manufacturer speed innovations to market

New 'everybike' reclaims the joy of your first two-wheeler

3D CAD models are faster to create and easier to comprehend

SOLIDWORKS 3D CAD software ensures 23-ton vehicle used to maintain troop and civilian safety around the world is 'more than meets the eye'

3D CAD software helps U.K. retail display company compete on creativity, time to market

Designed in SOLIDWORKS software, this lowriding sport machine becomes nimble highrider on demand

Designed and Tested in SOLIDWORKS and SOLIDWORKS Simulation, Each Reee Chair Made of Plastic From Nine Recycled Game Consoles

Trojan Technologies uses SOLIDWORKS 3D CAD and Simulation Software to Develop Disinfection Systems Based on UV Light

Twins from Maine Grow Up Tinkering, Become Prime Defense Contractors

Company Renowned for ‘Headset’ Innovation Cuts Time and Cost with SolidWorks Design and Simulation Software

Episode Showcases Performance of Straitline Components’ Custom Hydraulic Brake Line Detangler in JumpShip Competition

Luxury Bike Shop Eliminates Prototypes Using SOLIDWORKS Simulation Software

A Transform 2012 Dancelink team in Mondim, Portugal, clears the way for a Transform Transit team to see the Gospel bear fruit four months later.

Following the recent protests and riots, OM Panama's Mission Extreme group helps clean the Volcán police station.

God answers the prayers of OM Guatemala and a partnering church with a Christmas celebration for children and families with OM’s Project Rescue.

A testimony of change in a Malawian village

Ride 2 Transform takes its third tour of southern Malawi for ministry and support.

OM workers share how God answered prayers for visas to stay in North Africa.

A News24 Business reader has to pay R500 to receive a debt clearance certificate but is currently unemployed.

The Ombud for Short-term Insurance explains how motor vehicles are valued and what this implies for their cover,

Penn State Great Valley has unveiled a mural depicting the Penn State Nittany Lion, created from upcycled plastic bottle caps by mosaic studio artist Judith Hokky.  

An annual outreach attracts not only children but also two women, who listen attentively and respond to the message.

Stefano Terlizzi joined the Ken and Mary Alice Lindquist Department of Nuclear Engineering as the John and Jean M. Brennan Clean Energy Early Career Professor on July 22. In this Q&A, Terlizzi talks about his research background and interests, why he chose Penn State and more.

Current charging stations for electric vehicles, or EVs, can be expensive to install, and limited in the number of parking spaces they reach. Penn State engineering student Jonathan Smith and his team have spent the last three years creating a possible solution: smaller, mounted charging stations, which can move to cover as many as five parking spaces. As CEO of Streamline Charging, he’s used his Penn State education and customized coursework to get the team’s ideas to market. 

OM workers in the Arabian Peninsula testify to healing and deliverance miracles amongst Muslims.

Cleaning up after class turns into a moment of sharing between an OM worker and two North African women.

This week SA Money Report tries to understand eThekwini's surprising plan for nuclear power, and whether the ANC's RET faction has a hand in things.

One girl’s dream comes true, as she is now able to go to a village primary school, started by OM.

Massive distribution efforts by OM teams in Bangladesh over more than 10 years sowed gospel seeds and made the Bengali New Testament a #1 best seller.

Jessie Stein tells the story of God's faithfulness in the midst of learning a foreign language and the struggles of a broken family.

India has ambitious plans to set up nuclear reactors across the country, especially in states where thermal power plants have either completed its life, or where access to coal is a challenge.

India has ambitious plans to set up nuclear reactors across the country, especially in states where thermal power plants have either completed its life, or where access to coal is a challenge.

NASA’s Power to Explore Challenge calls on U.S. students to submit ideas for moon missions powered by radioisotope systems. The contest encourages kindergarten through Year 12 students to envision a mission, detail objectives, and apply their unique “power” to achieve mission success. Entries are due by January 31, 2025, and grand prize winners will tour NASA’s Glenn Research Center, gaining insight into the technology and science of space exploration.

Indiana Jones and the Great Circle received a gameplay deep dive Monday that detailed more of the game’s combat, exploration and puzzles. Publisher Bethesda also shed more light on the premise of the story that sees Indy join the chase for a priceless stolen relic.

Starlink has been looking to enter India for years and its plans got a major boost last month when New Delhi said it wouldn't auction spectrum for satellite broadband but rather award it administratively - just as Musk wanted. Rival Indian telecom billionaire Mukesh Ambani had wanted an auction.

She reshared a thoughtful note on the significance of 11/11, a day believed by many to amplify manifestations and focus on setting clear intentions

The event was part of a trend of young Chinese travelling on the cheap and spending as little as possible at a time of scarce job prospects, when wages are under pressure.

The Delaware Division of the Arts’ Mezzanine Gallery presents artist Colleen Zufelt’s exhibition, Recycle, Repurpose…RE-IMAGINE, showing March 4 through 25, 2022. Her exhibit will also be part of Art Loop Wilmington — the monthly self-guided tour of art in and around the city.

The Delaware Department of Justice’s Fraud & Consumer Protection Division announced Monday that a grand jury has indicted Susan Losey on multiple counts of theft with an aggregate loss of approximately $22,000. In particular, Losey is accused of using her cleaning business, Down & Dirty Cleaning Services, to defraud more than 50 victims, primarily in […]

The Delaware Department of Agriculture (DDA) announced that beginning on April 19, 2023, the Delaware Council on Farm & Food Policy (Council) will accept grant applications for the second cycle of the First State Food System Program. Through this program, funding is available to Delaware farmers and a diverse range of food supply chain entities. Awarded funds will aid local entities to expand capacity, coordinate with partners in their communities, and do their part to build resilience in Delaware’s food system.