Ryan Murphy and Ian Brennan rewrite the history of Hollywood in Netflix's "Hollywood," a progressive fantasy of motion pictures in 1947.

Rangers take on Kilmarnock in the Scottish Premiership on Wednesday night, with Steven Gerrard looking to keep within touching distance of rivals Celtic. Express Sport delivers you all of the team news for the Gers, as well as the predicted line up for the clash, as Ryan Jack returns from injury.

Leeds face Huddersfield in the Championship on Saturday, as Marcelo Bielsa aims to continue his the Whites' superb form at Elland Road. We bring you the latest team news and predicted line up, as the manager makes a key decision on whether to drop striker Patrick Bamford, as Kiko Casilla remains banned.

Manchester United are reportedly interested in signing Inter Milan striker Lautaro Martinez this summer.

The Premier League and the EFL have been urged to end the season with league winners and promotion but no relegation.

POPE BENEDICT is "making Francis' life a misery" as rumours of a major row between the two Vatican titans continue to boil, an expert has warned.

Leeds United take on Hull City on Saturday and Whites boss Marcelo Bielsa faces an important decision after Kiko Casilla was issued an eight-match ban.

Celtic host Hearts as top takes on bottom in the Scottish Premiership, with Neil Lennon hoping to maintain his seven-point lead over title rivals Rangers. Express Sport is on hand to provide all of the Hoops' team news, as well the predicted line-up, as two stars return from injury.

PARTICK THISTLE were left baffled after a goal was disallowed despite hitting the net during their clash with Greenock Morton.

Rafael Nadal has been outspoken with his thoughts on how the Spanish government handled the coronavirus pandemic.

Stoke striker Tyrese Campbell has garnered interest from a swathe of British clubs.

Liverpool are looking to make Sadio Mane an offer that would tarnish Real Madrid's hopes of signing him.

Leeds face Middlesbrough in the Championship on Wednesday evening, with Marcelo Bielsa hoping to continue the Whites' good form after back-to-back wins. Express Sport brings you the latest team news and injury updates, with concerns over the inclusion of Kalvin Phillips and Kiko Casilla in the manager's starting XI.

Leeds fans have demanded that Patrick Bamford is dropped by Marcelo Bielsa from his starting line-up, after the Whites striker missed a big chance to double the club's lead against Middlesbrough in the Championship, during their 1-0 win at the Riverside.

The Premier League will wait to hear from its players before putting a vote on the controversial neutral grounds protocol to clubs.

THANKFULLY, the Government is still encouraging us to get out and exercise - as long as we keep the two-metre distance rule.

The Premier League will wait to hear from its players before putting a vote on the controversial neutral grounds protocol to clubs.

The SPFL board are waiting from one club in the Championship to register their vote on the proposed resolution to end the season now.

ANDREW LLOYD WEBBER has written some of the greatest musicals of our time with lyricist Tim Rice - but what happened between them?

FOLLOWING the Windrush scandal the NHS says that its records will no longer be used to track down illegal immigrants.

Chelsea news and gossip is coming in thick and fast so Express Sport is on hand to bring you all the very latest from Stamford Bridge.

The Premier League will wait to hear from its players before putting a vote on the controversial neutral grounds protocol to clubs.

We all must work together to dismantle the barriers and empower our neighbors for a truly inclusive city, a letter to the editor says.

Indiana University grad Hannah Fidell directed 'The Long Dumb Road,' closing-night movie at this year's Indy Film Fest.

As Indiana prepares to reopen its economy during coronavirus, parents who are returning to work are facing a new challenge about childcare.

The private Catholic university in South Bend with $11 billion in reserves follows similar decisions by other endowment-rich institutions.

Holcomb and Hogsett say they are on the same page.

Starting May 12, the L.A. County supervisors will allow the public to comment live for the first time since the coronavirus ended in-person meetings.

The director of the San Francisco Department of Public Health told residents Wednesday not to visit their mothers on Mother's Day, even if wearing masks and keeping six feet apart.

Simon executives are furious at Indianapolis officials.

How do we keep fit while heeding the government's new advice on avoiding unnecessary social contact?

Men with advanced prostate cancer can now take highly targeted hormone therapies at home.

Even two filmmaking legends can cast the wrong villains amid massive industry change.

In an emotional interview with TMZ, the comedian said she canceled her show there because of the state's attempt to, in effect, ban abortion.

Sadly, the most recent horrors are not an aberration.

The Amateur Traveler talks to Richard Miller of the SparkleTack podcast (about San Francisco history) about San Francisco. Richard guides us in a driving/walking tour from west to east. We start at lands end and end up near fisherman's wharf while talking about the sites and history of many of the places in between. This is a great companion episode with the 2 part soundseeing walking tour of San Francisco.

Hear about travel to San Francisco, California as the Amateur Traveler talks to Jill Robinson and Kimberley Levato authors of 100 Things to Do in San Francisco Before You Die.

Danny Jordaan says it would be massively complicated to ensure the safety of supporters should the PSL return while Covid-19 is still prevalent in South Africa.

Decision-making can be tough especially when the best choice isn’t glaringly obvious. We’re faced with this process every minute of the day. When do I wake up? What’s for lunch? Do I want to go out on a date with this person? What do I want to do with my life? Can I stay in […]

The post Stop Losing Sleep: 7 Steps for Stress-free Decision-making appeared first on Dumb Little Man.

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The post VIDEO: GOP senator says China made ‘conscious decision’ to allow COVID-19 to spread beyond border appeared first on Dennis Michael Lynch.

Any plans to visit the Narcisse Snake Dens this Mother's Day weekend will have to be put on hold, after the province announced they are closed until further notice.

The arrangement of functionally-related genes in operons is a fundamental element of how genetic information is organized in prokaryotes. This organization ensures coordinated gene expression by co-transcription. Often, however, alternative genetic responses to specific stress conditions demand the discoordination of operon expression. During cold temperature stress, accumulation of the gene encoding the sole Asp–Glu–Ala–Asp (DEAD)-box RNA helicase in Synechocystis sp. PCC 6803, crhR (slr0083), increases 15-fold. Here, we show that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/miaB (slr0082) gene, followed by rapid processing of the operon transcript into two monocistronic mRNAs. This cleavage event is required for and results in destabilization of the rimO transcript. Results from secondary structure modeling and analysis of RNase E cleavage of the rimO–crhR transcript in vitro suggested that CrhR plays a role in enhancing the rate of the processing in an auto-regulatory manner. Moreover, two putative small RNAs are generated from additional processing, degradation, or both of the rimO transcript. These results suggest a role for the bacterial RNA helicase CrhR in RNase E-dependent mRNA processing in Synechocystis and expand the known range of organisms possessing small RNAs derived from processing of mRNA transcripts.

Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective processing of single cells. In particular, the concept of using boosting/carrier channels in isobaric labeling to increase the sensitivity in MS detection has also been increasingly used for quantitative proteomic analysis of small-sized samples including single cells. However, the full potential of such boosting/carrier approaches has not been significantly explored, nor has the resulting quantitation quality been carefully evaluated. Herein, we have further evaluated and optimized our recent boosting to amplify signal with isobaric labeling (BASIL) approach, originally developed for quantifying phosphorylation in small number of cells, for highly effective analysis of proteins in single cells. This improved BASIL (iBASIL) approach enables reliable quantitative single-cell proteomics analysis with greater proteome coverage by carefully controlling the boosting-to-sample ratio (e.g. in general <100x) and optimizing MS automatic gain control (AGC) and ion injection time settings in MS/MS analysis (e.g. 5E5 and 300 ms, respectively, which is significantly higher than that used in typical bulk analysis). By coupling with a nanodroplet-based single cell preparation (nanoPOTS) platform, iBASIL enabled identification of ~2500 proteins and precise quantification of ~1500 proteins in the analysis of 104 FACS-isolated single cells, with the resulting protein profiles robustly clustering the cells from three different acute myeloid leukemia cell lines. This study highlights the importance of carefully evaluating and optimizing the boosting ratios and MS data acquisition conditions for achieving robust, comprehensive proteomic analysis of single cells.

The arrangement of functionally-related genes in operons is a fundamental element of how genetic information is organized in prokaryotes. This organization ensures coordinated gene expression by co-transcription. Often, however, alternative genetic responses to specific stress conditions demand the discoordination of operon expression. During cold temperature stress, accumulation of the gene encoding the sole Asp–Glu–Ala–Asp (DEAD)-box RNA helicase in Synechocystis sp. PCC 6803, crhR (slr0083), increases 15-fold. Here, we show that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/miaB (slr0082) gene, followed by rapid processing of the operon transcript into two monocistronic mRNAs. This cleavage event is required for and results in destabilization of the rimO transcript. Results from secondary structure modeling and analysis of RNase E cleavage of the rimO–crhR transcript in vitro suggested that CrhR plays a role in enhancing the rate of the processing in an auto-regulatory manner. Moreover, two putative small RNAs are generated from additional processing, degradation, or both of the rimO transcript. These results suggest a role for the bacterial RNA helicase CrhR in RNase E-dependent mRNA processing in Synechocystis and expand the known range of organisms possessing small RNAs derived from processing of mRNA transcripts.

24 January 2020