TERRA Engineering is seeking an experienced Project Engineer to join our Site Development Department in our corporate headquarters in Chicago, Illinois. Job Description: Engineer will be responsible for the preparation of design plans, details, construction budget, code compliance, design calcula

Woolpert, Inc. is a cutting-edge national architecture, engineering and geospatial (AEG) firm that delivers value to clients by blending engineering excellence with innovative technology and geospatial applications. Woolpert was recently certified as an Employer of Choice by Great Place To Work .

Woolpert, Inc. is a cutting-edge national architecture, engineering and geospatial (AEG) firm that delivers value to clients by blending engineering excellence with innovative technology and geospatial applications. Woolpert was recently certified as an Employer of Choice by Great Place To Work .

Woolpert, Inc. is a cutting-edge national architecture, engineering and geospatial (AEG) firm that delivers value to clients by blending engineering excellence with innovative technology and geospatial applications. Woolpert was recently certified as an Employer of Choice by Great Place To Work .

Woolpert, Inc. is a cutting-edge national architecture, engineering and geospatial (AEG) firm that delivers value to clients by blending engineering excellence with innovative technology and geospatial applications. Woolpert was recently certified as an Employer of Choice by Great Place To Work .

Woolpert, Inc. is a cutting-edge national architecture, engineering and geospatial (AEG) firm that delivers value to clients by blending engineering excellence with innovative technology and geospatial applications. Woolpert was recently certified as an Employer of Choice by Great Place To Work .

The Church of Jesus Christ of Latter-day Saints plans a four-phase reopening of its 167 temples worldwide that were closed due to the coronavirus.

You can have a sneak preview at the new Tripadvisor site by browsing this “beta” microsite that TA globally announced today in its newsletter. The user-generated reviews’ giant Tripavisor (part of the Expedia, Inc. family of travel companies), decided to “create a new and easier to use” site. Kristen Nicole from Mashable previously published an article [...]

IMPD Chief Randal Taylor and Dreasjon Reed's family speak during emotional meeting, near where Reed was killed Wednesday in police-action shooting.

Indiana coronavirus cases and newly reported deaths from COVID-19 for Friday, May 8.

Vanessa Bryant has filed a claim against the Los Angeles County Sheriff's Department over deputies sharing "unauthorized" photos of the scene of the helicopter crash that killed her husband Kobe Bryant, their daughter and seven others.

The Universite de Montreal is the first in the city to announce that most of its fall courses will be delivered at a distance as a response to the continuing COVID-19 crisis.

If you are just starting up your website, perhaps you are selling tangible items, or you have a hit single that you’ll be offering digital copies of, one of your pressing questions may be: “Which payment gateway should I use to accept payments from my customers?“. While there are pros and cons of using any […]

The post Benefits of Adding Multiple Payment Gateways to Your Website’s Checkout appeared first on Tips and Tricks HQ.

Any economic improvements aren’t helped by his actions.

Staying fit these days is now more important than ever with the global pandemic having no cure or vaccine yet other than a healthy body. It just so happens that being active is also more difficult because gyms are closed in affected areas. Home workouts like yoga, in that regard, are golden and yoga website […]

The post 12 Yoga Website Designs for Inspiration appeared first on SpyreStudios.

ECommerce is undeniably a buzzword. Many people have been discussing this term for a long time since the Internet was opened to commercial use. Through its history, with many advantages over brick and mortar stores as well as mail-order catalogs, eCommerce has become one of the fastest-growing industries.  Did you know that 25% of the […]

The post What Will An Ecommerce Website Need To Stand Out In 2020? appeared first on SpyreStudios.

Meghan Markle is reportedly set to launch her lifestyle website to rival Gwyneth Paltrow's Goop.

Modern commerce is a highly complex global business that is always evolving – and the pace of change is only accelerating. If you work in digital commerce, it’s crucial to stay up-to-date with the latest developments in technology, business, shopper behavior and trends, laws and regulations, payments, logistics and more. How can you stay on […]

The Amateur Traveler talks about a recent trip to Maui as the guest of the Maui Convention and Visitors Bureau. In this first half of a two part episode Chris gives an overview of the island and talks about the Hawai’i Nature Center, the beaches and the road to Hana. Learn about swimming or hiking in lava tubes, hidden beaches and some of the naive plants of Maui.

Show Notes

Visit Maui Blog
Rainfall map of Maui
Wailea Beach Marriott Resort & Spa
The Ritz-Carlton, Kapalua
Kamehameha I
Hawaiian Language

Beaches:

    * Makena (big beach)
    * Chang’s Beach
    * ‘Ahihi Bay
    * Maluaka Beach
    * Wai’ anapanapa State Park
    * Ka’eleku Caverns

The Amateur Traveler talks about a recent trip to Maui as the guest of the Maui Convention and Visitors Bureau. In this second half of a two part episode Chris talks about some of the local culture, food and festivals. He had a chance to explore his rancher roots at one of Maui’s cattle ranches, dine on local produce at some high end restaurants, mix with the locals at the Maui Ag Festival and learn more of Maui’s culture at the Celebration of the Arts.

The Amateur Traveler talks to Gary Arndt of Everything-Everywhere.com  about UNESCO World Heritage Sites. The World Heritage List includes 890 sites from around the world which are part of the cultural and natural heritage which the World Heritage Committee considers as having “outstanding universal value”. We talk about various sites as well as how to use this list as a resource in your travels.

Hear about all of Canada's UNESCO World Heritage Sites as the Amateur Traveler talks again to Gary Arndt of Everything-Everywhere.com about traveling to all 17 sites. Some sites like Old Québec are easy to reach and others like Wood Buffalo and Nahanni National Parks (the last two that Gary visited) are quite remote.

Hear about travel to Israel and Palestine, and particularly the world heritage sites of that region, as the Amateur Traveler talks to Gary Arndt from everything-everywhere.com about his recent visit.
Gary just finished up a trip to Israel and Palestine.

Hear about the United States UNESCO World Heritage Sites as the Amateur Traveler talks to Gary Arndt from Everything-Everywhere.com and The Global Travel Conspiracy podcast about these best of the best sites.

Hear about travel to Switzerland as the Amateur Traveler talks to Gary Arndt from everything-everywhere.com about his recent trip to visit all the UNESCO sites in Switzerland.

So you've decided to hike Machu Picchu? Well great! There's no doubt that you're in for the experience of a lifetime. However, the truth of the matter is that now you've got some additional decisions to make. You see, there's more than one route to arrive to the world-famous Inca site, and even once you've arrived there are a few choices to make as well.

But don't fret! If you're looking to hike Machu Picchu, we've got the information you need to start planning your trip.

Hike Machu Picchu: Arriving to the Site

1. Classic Inca Trail

The Classic Inca Trail is a four-day trek stretching some 42 kilometers along an ancient road built by the Incas themselves. This is undoubtedly the most famous route to Machu Picchu, and it's the one way that offers the opportunity to enter the city through its storied Sun Gate. Frankly, we've got tons of information on the Inca Trail on other pages of our site, so we won't waste too much space being redundant here. Check out these pages for more information on the trail's itinerary as well as for access to an Inca Trail map.

2. One-Day Inca Trail

Whether you're crunched for time or simply don't feel physically up to four full days of trekking, don't fret--a small taste of the world-famous Inca Trail is still available to you! A number of tour companies offer an abridged version of the trek, either one full day of hiking or a shortened day followed by a night of camping and a dawn arrival at Machu Picchu. Besides the Inca Trail's famed destination, the one-day version of the trek also takes you past Wiñay Wayna, another favorite ruins along the trail. The bad news here is that you'll still need to reserve one of only a few hundred daily Inca Trail passes, meaning that, just like the full Inca Trail, you'll need to book this trek months in advance.

3. Lares Trail

Just north of the world-famous Sacred Valley sits the comparatively lesser-known Lares Valley, and whereas the former can be swarmed with tourists especially during the high season, life in the Lares Valley continues humming along relatively unchanged. So whether you're looking for a simpler, more culturally-immersive experience or if you just didn't book an Inca Trail pass in time, the Lares Trail is an excellent option! Lucky for you, we've already gathered plenty of information on the Lares Trail including the trek's daily itinerary and a side-by-side comparison of the Lares and Inca Trails if you're struggling to decide how to hike Machu Picchu.

4. Vilcabamba Traverse Route

Warning--this option is neither for the faint of heart nor the out of shape! The Vilcabamba Traverse Route is one of the newest on the Peru trekking circuit, clocking in at nearly 100 kilometers over the course of a week of trekking. Though very difficult, the route is especially rewarding--think a variety of diverse biomes and the ruins of Choquequirao, often compared to Machu Picchu although less than 40% of the site has been excavated (pictured above). Though visited by only 5,000 or so brave trekkers last year, be warned--the local government is pushing a plan to build a cable car to the site! If you want to see the ruins in their current, undisturbed state, it would be best to go sooner than later.

Hike Machu Picchu: Once You're There

5. Huayna Picchu

Just because you've finally arrived at Machu Picchu doesn't mean your hiking experience has to end! The famous mountain soaring in the background of most iconic Machu Picchu photos is possible to hike, and the experience is certainly recommended. The views from the top are great, and on the way down you can even take a back route to the storied Temple of the Moon. For more information on Huayna Picchu, you can check out this past post on our site.

So that's it for today! If you're planning to hike Machu Picchu and have any questions you'd like to ask us, feel free to reach out in the comments section below. And, if you've already done one of these hikes, feel free to share your experience as well. We appreciate all of your comments!

Essex County OPP have released a composite drawing of a suspect reported to be impersonating a police officer in Lakeshore.

Ivan Matic
Jan 1, 2008; 7:132-144
Research

Yi Zhang
Sep 1, 2005; 4:1240-1250
Research

Sebastian A. Wagner
Oct 1, 2011; 10:M111.013284-M111.013284
Research

Yu Xue
Sep 1, 2008; 7:1598-1608
Research

Heme-regulatory motifs (HRMs) are present in many proteins that are involved in diverse biological functions. The C-terminal tail region of human heme oxygenase-2 (HO2) contains two HRMs whose cysteine residues form a disulfide bond; when reduced, these cysteines are available to bind Fe3+-heme. Heme binding to the HRMs occurs independently of the HO2 catalytic active site in the core of the protein, where heme binds with high affinity and is degraded to biliverdin. Here, we describe the reversible, protein-mediated transfer of heme between the HRMs and the HO2 core. Using hydrogen-deuterium exchange (HDX)-MS to monitor the dynamics of HO2 with and without Fe3+-heme bound to the HRMs and to the core, we detected conformational changes in the catalytic core only in one state of the catalytic cycle—when Fe3+-heme is bound to the HRMs and the core is in the apo state. These conformational changes were consistent with transfer of heme between binding sites. Indeed, we observed that HRM-bound Fe3+-heme is transferred to the apo-core either upon independent expression of the core and of a construct spanning the HRM-containing tail or after a single turnover of heme at the core. Moreover, we observed transfer of heme from the core to the HRMs and equilibration of heme between the core and HRMs. We therefore propose an Fe3+-heme transfer model in which HRM-bound heme is readily transferred to the catalytic site for degradation to facilitate turnover but can also equilibrate between the sites to maintain heme homeostasis.

The quinoprotein glycine oxidase from the marine bacterium Pseudoalteromonas luteoviolacea (PlGoxA) uses a protein-derived cysteine tryptophylquinone (CTQ) cofactor to catalyze conversion of glycine to glyoxylate and ammonia. This homotetrameric enzyme exhibits strong cooperativity toward glycine binding. It is a good model for studying enzyme kinetics and cooperativity, specifically for being able to separate those aspects of protein function through directed mutagenesis. Variant proteins were generated with mutations in four active-site residues, Phe-316, His-583, Tyr-766, and His-767. Structures for glycine-soaked crystals were obtained for each. Different mutations had differential effects on kcat and K0.5 for catalysis, K0.5 for substrate binding, and the Hill coefficients describing the steady-state kinetics or substrate binding. Phe-316 and Tyr-766 variants retained catalytic activity, albeit with altered kinetics and cooperativity. Substitutions of His-583 revealed that it is essential for glycine binding, and the structure of H583C PlGoxA had no active-site glycine present in glycine-soaked crystals. The structure of H767A PlGoxA revealed a previously undetected reaction intermediate, a carbinolamine product-reduced CTQ adduct, and exhibited only negligible activity. The results of these experiments, as well as those with the native enzyme and previous variants, enabled construction of a detailed mechanism for the reductive half-reaction of glycine oxidation. This proposed mechanism includes three discrete reaction intermediates that are covalently bound to CTQ during the reaction, two of which have now been structurally characterized by X-ray crystallography.

Expert

The leak exposed millions of records with full names, emails, user conversations, payment logs, and IP addresses dating back to March.

Researcher: Jackson Cothren, University of Arkansas
Moment Title: Scanning Ancient Sites
Description: Jackson Cothren talks about creating three-dimensional scans of ancient sites.