Der Dokumentarfotograf Steve McCurry reist seit mehr als 40 Jahren durch die Welt. Jeder kennt sein „afghanisches Mädchen“. Nun gewährt eine visuelle Biografie den bislang persönlichsten Einblick in sein Schaffen.

Greetings, Sapients! You may have visited famed site "Metafilter" before - but did you know this: Metafilter also has "Metafilter Chat"! It does.

As this chat is associated with Metafilter proper, we chat about all kinds of things! Do you like cute animal GIFs? We chat those! Do you wish to ask a question? We answer! Do you fucking fuck the fucking thing? We fuck that!

As implied above, you may chat through the standard web interface. If you wish to use alternate methods of chatting, there are OPTIONS! We are very quite versatile, you know. [previously]

Prithee, come chat. We do not bite - unless you ask!

Rock Mafia is an American record production/songwriting team, consisting of Tim James and Antonina Armato who have been active since the early 2000s. They have worked with artists such as Demi Lovato, Vanessa Hudgens, Miley Cyrus, Selena Gomez, No Doubt, Wyclef Jean, Green Day, Mariah Carey, Justin Bieber, Flo Rida, Ellie Goulding, A Great Big World, Tokio Hotel, Bebe Rexha, Armin van Buuren, and Laura Marano. Rock Mafia have sold over 100 million albums worldwide. We are seeking the best new artists, songwriters, and producers to add to our growing team to help with new projects. Please submit your best track(s) for consideration.

- Rock Mafia

Deal Type: Label / Management Signing / Collaboration
Decision Maker: I'm the final decision maker
Deal Structure: Negotiable
Compensation: Negotiable
Song Quality: Rough Mixes, Fully mastered, Broadcast ready
Similar Sounding Artists: Demi Lovato, Vanessa Hudgens, Miley Cyrus, Selena Gomez, No Doubt, Wyclef Jean, Green Day, Mariah Carey, Justin Bieber, Flo Rida, Ellie Goulding, A Great Big World, Tokio Hotel, Bebe Rexha, Armin van Buuren, and Laura Marano

http://www.musicxray.com/xrays/1319835 HUNNAFIEDRECORDS - Hunnafied records - blood money

The pandemic has shut down graduation celebrations across nation. On Tuesday, Facebook and Instagram announced plans to celebrate this year's graduating class with a streaming event on May 15, featuring Oprah Winfrey as commencement speaker.

An artist harder to define, and more brilliant, than you might imagine.

John Lafia, who co-wrote 1988 horror film "Child's Play" and also co-wrote and directed "Child's Play 2," died April 29 in Los Angeles. He was 63. Lafia collaborated with Tom Holland and Don Mancini on the horror movie screenplay, and was credited with coining the name "Chucky" and contributed the famous line, "Hi, I'm Chucky, […]

(California Court of Appeal) - Held that a pain management physician did not have to produce the medical records of five of his patients pursuant to a subpoena issued by an investigator with the Medical Board of California, because the board did not establish good cause for the subpoena. Reversed the decision below, in this case involving prescriptions for controlled substances.

The battle is set to go down this Thursday at 8 PM ET.

v. Sober

or Someone or something that has been thoroughly disinfected.

I drank some coffee, Im good and decoronafied, can I have my keys?.Honest I can drive !
or Babe ! Im home! let me take a shower and get decoronafied so I can smash that !

2k has a new promotion during the pandemic they are offering a free game each week. I have little info about this but I'm playing mafia 3 on xbox one currently. feel free to post with details if you want. I barely remeber playing this on last gen, I think I played a few hours and quit. now though I'm really enjoying it as a time-filler, it's a good gta rip-off.

August 3rd, 2019
Afisha Picnic in Russia
Scheduled to play from 8:30 - 11 pm.

In the US that's a start time of 1:30 pm eastern, 12:30 pm central, 11:30 am mountain, 10:30 am pacific.

Setlist: Plainsong, Pictures of You, High, Just One Kiss, Lovesong, Last Dance, Burn, Fascination Street, Never Enough, Push, In Between Days, Just Like Heaven, From the Edge of the Deep Green Sea, Shake Dog Shake, A Night Like This, Play For Today, A Forest, Primary, 39, Disintegration

Encore: Lullaby, The Caterpillar, The Walk, Friday I'm in Love, Close to Me, Why Can't I Be You?, Boys Don't Cry.

John Lafia, who co-wrote the 1988 horror movie "Child's Play" and its 1990 sequel, died on April 29. He was 63.

Prominins (proms) are transmembrane glycoproteins conserved throughout the animal kingdom. They are associated with plasma membrane protrusions, such as primary cilia, as well as extracellular vesicles derived thereof. Primary cilia host numerous signaling pathways affected in diseases known as ciliopathies. Human PROM1 (CD133) is detected in both somatic and cancer stem cells and is also expressed in terminally differentiated epithelial and photoreceptor cells. Genetic mutations in the PROM1 gene result in retinal degeneration by impairing the proper formation of the outer segment of photoreceptors, a modified cilium. Here, we investigated the impact of proms on two distinct examples of ciliogenesis. First, we demonstrate that the overexpression of a dominant-negative mutant variant of human PROM1 (i.e. mutation Y819F/Y828F) significantly decreases ciliary length in Madin–Darby canine kidney cells. These results contrast strongly to the previously observed enhancing effect of WT PROM1 on ciliary length. Mechanistically, the mutation impeded the interaction of PROM1 with ADP-ribosylation factor–like protein 13B, a key regulator of ciliary length. Second, we observed that in vivo knockdown of prom3 in zebrafish alters the number and length of monocilia in the Kupffer's vesicle, resulting in molecular and anatomical defects in the left-right asymmetry. These distinct loss-of-function approaches in two biological systems reveal that prom proteins are critical for the integrity and function of cilia. Our data provide new insights into ciliogenesis and might be of particular interest for investigations of the etiologies of ciliopathies.

Prominins (proms) are transmembrane glycoproteins conserved throughout the animal kingdom. They are associated with plasma membrane protrusions, such as primary cilia, as well as extracellular vesicles derived thereof. Primary cilia host numerous signaling pathways affected in diseases known as ciliopathies. Human PROM1 (CD133) is detected in both somatic and cancer stem cells and is also expressed in terminally differentiated epithelial and photoreceptor cells. Genetic mutations in the PROM1 gene result in retinal degeneration by impairing the proper formation of the outer segment of photoreceptors, a modified cilium. Here, we investigated the impact of proms on two distinct examples of ciliogenesis. First, we demonstrate that the overexpression of a dominant-negative mutant variant of human PROM1 (i.e. mutation Y819F/Y828F) significantly decreases ciliary length in Madin–Darby canine kidney cells. These results contrast strongly to the previously observed enhancing effect of WT PROM1 on ciliary length. Mechanistically, the mutation impeded the interaction of PROM1 with ADP-ribosylation factor–like protein 13B, a key regulator of ciliary length. Second, we observed that in vivo knockdown of prom3 in zebrafish alters the number and length of monocilia in the Kupffer's vesicle, resulting in molecular and anatomical defects in the left-right asymmetry. These distinct loss-of-function approaches in two biological systems reveal that prom proteins are critical for the integrity and function of cilia. Our data provide new insights into ciliogenesis and might be of particular interest for investigations of the etiologies of ciliopathies.

Prominins (proms) are transmembrane glycoproteins conserved throughout the animal kingdom. They are associated with plasma membrane protrusions, such as primary cilia, as well as extracellular vesicles derived thereof. Primary cilia host numerous signaling pathways affected in diseases known as ciliopathies. Human PROM1 (CD133) is detected in both somatic and cancer stem cells and is also expressed in terminally differentiated epithelial and photoreceptor cells. Genetic mutations in the PROM1 gene result in retinal degeneration by impairing the proper formation of the outer segment of photoreceptors, a modified cilium. Here, we investigated the impact of proms on two distinct examples of ciliogenesis. First, we demonstrate that the overexpression of a dominant-negative mutant variant of human PROM1 (i.e. mutation Y819F/Y828F) significantly decreases ciliary length in Madin–Darby canine kidney cells. These results contrast strongly to the previously observed enhancing effect of WT PROM1 on ciliary length. Mechanistically, the mutation impeded the interaction of PROM1 with ADP-ribosylation factor–like protein 13B, a key regulator of ciliary length. Second, we observed that in vivo knockdown of prom3 in zebrafish alters the number and length of monocilia in the Kupffer's vesicle, resulting in molecular and anatomical defects in the left-right asymmetry. These distinct loss-of-function approaches in two biological systems reveal that prom proteins are critical for the integrity and function of cilia. Our data provide new insights into ciliogenesis and might be of particular interest for investigations of the etiologies of ciliopathies.

Jared W. Young
Mar 4, 2009; 29:2663-2665
Journal Club

Spanish translation of the BIS press release on the presentation of the Annual Economic Report 2019, 30 June 2019.

The cerebellum influences motor control through Purkinje target neurons, which transmit cerebellar output. Such output is required, for instance, for larval zebrafish to learn conditioned fictive swimming. The output cells, called eurydendroid neurons (ENs) in teleost fish, are inhibited by Purkinje cells and excited by parallel fibers. Here, we investigated the electrophysiological properties of glutamatergic ENs labeled by the transcription factor olig2. Action potential firing and synaptic responses were recorded in current clamp and voltage clamp from olig2⁺ neurons in immobilized larval zebrafish (before sexual differentiation) and were correlated with motor behavior by simultaneous recording of fictive swimming. In the absence of swimming, olig2⁺ ENs had basal firing rates near 8 spikes/s, and EPSCs and IPSCs were evident. Comparing Purkinje firing rates and eurydendroid IPSC rates indicated that 1-3 Purkinje cells converge onto each EN. Optogenetically suppressing Purkinje simple spikes, while preserving complex spikes, suggested that eurydendroid IPSC size depended on presynaptic spike duration rather than amplitude. During swimming, EPSC and IPSC rates increased. Total excitatory and inhibitory currents during sensory-evoked swimming were both more than double those during spontaneous swimming. During both spontaneous and sensory-evoked swimming, the total inhibitory current was more than threefold larger than the excitatory current. Firing rates of ENs nevertheless increased, suggesting that the relative timing of IPSCs and EPSCs may permit excitation to drive additional eurydendroid spikes. The data indicate that olig2⁺ cells are ENs whose activity is modulated with locomotion, suiting them to participate in sensorimotor integration associated with cerebellum-dependent learning.

SIGNIFICANCE STATEMENT The cerebellum contributes to movements through signals generated by cerebellar output neurons, called eurydendroid neurons (ENs) in fish (cerebellar nuclei in mammals). ENs receive sensory and motor signals from excitatory parallel fibers and inhibitory Purkinje cells. Here, we report electrophysiological recordings from ENs of larval zebrafish that directly illustrate how synaptic inhibition and excitation are integrated by cerebellar output neurons in association with motor behavior. The results demonstrate that inhibitory and excitatory drive both increase during fictive swimming, but inhibition greatly exceeds excitation. Firing rates nevertheless increase, providing evidence that synaptic integration promotes cerebellar output during locomotion. The data offer a basis for comparing aspects of cerebellar coding that are conserved and that diverge across vertebrates.

<span class="topsy_trackback_comment"><span class="topsy_twitter_username"><span class="topsy_trackback_content"></span></span>

I rapporti tra Gheddafi e Déby sono caratterizzati da una certa superficialità, dovuta alle tensioni passate e ai sospetti che nutrono l’uno per l’altro.

The fall of Qaddafi’s regime, followed by his death on 20 October, could pave the way to promises of democracy in Libya but left neighbouring countries facing new potential problems that could threaten stability in the region.

The cockpit glasses promise to give you a bird's eye view from the Anafi drone's 180-degree camera. The Anafi FPV bundle will go on sale early this month.

New Castle, Del. (August 14, 2017) – A founding scientist and previous vice president of the pharmaceutical company Incyte is growing his next venture in Delaware. Dr. Kris Vaddi started Prelude Therapeutics in July 2016 with the vision of creating a leading cancer drug discovery-based biotech company. Prelude currently has about a dozen people working […]

Union Bank received the stake after Andhra Bank was merged with the Mumbai-based lender, effective April 1.

Hi,

I want to convert ircx file(which is from TSMC,inclued EM Information) to ict or emDataFile for Voltus-fi.

I tried many way, but I can not make it. Can anyone give me some advice?

and I  do not installed QRC.

below is some tools installed my server. 

IC617-64b.500.21 is used.

Hi,

I want to convert ircx file(which from TSMC) to ict or emDataFile for Voltus-fi.

I tried many way, but I can not make it.

and I  do not installed QRC.

below is some tools installed my server. 

IC617-64b.500.21 is used.

Abstract: This articles reviews the Court of Appeal decision in Serafin v Malkiewicz reversing the dismissal at first instance of a libel claim relating to an article which made serious allegations about the claimant.  The ruling makes clear th...

Soomaaliya waxa ay guddoontay maalintii Khamiis ahayd (7 May) shixnaddii labaad oo ah kaalmo agab caafimaad oo ay ku deeqday Dowladda Jamhuuriyadda Shacbiga Shiinaha si looga hortago COVID-19, iyadooo ka koobnayd maaskaro, galoofyo, dareeraha gacmaha lagu nadiifiyo, qalabka baaridda-caafimaad iyo dharka ilaalinta leh ee loogu talogalay in ay ku labistaan xirfadlayaasha caafimaadka. Tan iyo 24 […]

The post Shiinaha oo Soomaaliya soo gaarsiiyey deeq caafimaad appeared first on Horseed Media • Somali News.

Title: zafirlukast (Accolate)
Category: Medications
Created: 4/19/1999 12:00:00 AM
Last Editorial Review: 11/21/2019 12:00:00 AM

Title: Afinitor Approval Expanded to Include Benign Kidney Tumors
Category: Health News
Created: 4/27/2012 2:05:00 PM
Last Editorial Review: 4/30/2012 12:00:00 AM

Title: Cialis (tadalafil) vs. Viagra (sildenafil)
Category: Medications
Created: 10/30/2017 12:00:00 AM
Last Editorial Review: 2/12/2020 12:00:00 AM

Progression from the initial vascular response upon hyperglycemia to a proliferative stage with neovacularizations is the hallmark of proliferative diabetic retinopathy. Here, we report on the novel diabetic pdx1^–/– zebrafish mutant as a model for diabetic retinopathy that lacks the transcription factor pdx1 through CRISPR-Cas9–mediated gene knockout leading to disturbed pancreatic development and hyperglycemia. Larval pdx1^–/– mutants prominently show vasodilation of blood vessels through increased vascular thickness in the hyaloid network as direct developmental precursor of the adult retinal vasculature in zebrafish. In adult pdx1^–/– mutants, impaired glucose homeostasis induces increased hyperbranching and hypersprouting with new vessel formation in the retina and aggravation of the vascular alterations from the larval to the adult stage. Both vascular aspects respond to antiangiogenic and antihyperglycemic pharmacological interventions in the larval stage and are accompanied by alterations in the nitric oxide metabolism. Thus, the pdx1^–/– mutant represents a novel model to study mechanisms of hyperglycemia-induced retinopathy wherein extensive proangiogenic alterations in blood vessel morphology and metabolic alterations underlie the vascular phenotype.

Nayeli G. Reyes-Nava, Hung-Chun Yu, Curtis R. Coughlin II, Tamim H. Shaikh, and Anita M. Quintana

We used whole-exome sequencing (WES) to determine the genetic etiology of a patient with a multi-system disorder characterized by a seizure phenotype. WES identified a heterozygous de novo missense mutation in the GABRA1 gene (c.875C>T). GABRA1 encodes the alpha subunit of the gamma-aminobutyric acid receptor A (GABA_AR). The GABA_AR is a ligand gated ion channel that mediates the fast inhibitory signals of the nervous system, and mutations in the subunits that compose the GABA_AR have been previously associated with human disease. To understand the mechanisms by which GABRA1 regulates brain development, we developed a zebrafish model of gabra1 deficiency. gabra1 expression is restricted to the nervous system and behavioral analysis of morpholino injected larvae suggests that the knockdown of gabra1 results in hypoactivity and defects in the expression of other subunits of the GABA_AR. Expression of the human GABRA1 protein in morphants partially restored the hypomotility phenotype. In contrast, the expression of the c.875C>T variant did not restore these behavioral deficits. Collectively, these results represent a functional approach to understand the mechanisms by which loss-of-function alleles cause disease.

One of our favorite consumer drones gets an FPV upgrade

2k has a new promotion during the pandemic they are offering a free game each week. I have little info about this but I'm playing mafia 3 on xbox one currently. feel free to post with details if you want. I barely remeber playing this on last gen, I think I played a few hours and quit. now though I'm really enjoying it as a time-filler, it's a good gta rip-off.

Arsenal defender Shkodran Mustafi has announced a birthday donation of 16,000 meals to help vulnerable people in Islington.

Marco Bellocchio’s mafia movie The Traitor topped last night’s David di Donatello Awards, Italy’s national film awards, scooping six prizes including Best Film. The movie also won Director for Bellocchio, Screenplay, Actor for Pierfrancesco Favino, Supporting Actor for Luigi Lo Cascio, and Best Editing. It premiered at Cannes last year before going on to gross […]

It is one of the most popular and important websites for cattle producers, rangers and carbon farmers in northern Australia, but its funding is about to run out.

(MedPage Today) -- Outcomes after radiofrequency (RF) ablation with a contact force-sensing catheter were promising for people with persistent atrial fibrillation (Afib) in the PRECEPT study. The device yielded 61.7% freedom at 15 months from...

(MedPage Today) -- A "hybrid convergent" surgical-plus-transcatheter procedure was better than endocardial catheter ablation alone for treating refractory atrial fibrillation (Afib) in the CONVERGE randomized trial. Additional ablation of the...

As the COVID-19 pandemic continues, many healthcare facilities around the world are inundated with critically ill patients, and resources such as equipment and staff are stretched thin. Shortages of critical equipment, such as ventilators, can mean the difference between life and death for patients, and the need to keep critically ill patients comfortable and alive […]

Hydroxychloroquine continues to feature in coronavirus news. Rick Bright, the Director of BARDA, alleges he was pushed out of his position due to his pushback to the administration focusing on the use of hydroxychloroquine in COVID-19 patients. This came after the National Institutes of Health said coronavirus patients should not take the drug due to potential “toxicities.”