Wildfires leave behind a patchwork of forest densities that can give bats more room to fly and hunt

The Spanish Surrealist painted a series of 100 watercolors inspired by Dante's "Divine Comedy"

Similarities between lunar samples and Earth's makeup were throwing off a leading theory of the moon's origin

The cats also cross the road an average of 4.5 times in six days, putting themselves in danger

New research reveals a Tang Dynasty woman's love for sports—and big-eared, braying equids

Tour world-class museums, read historic cookbooks, browse interactive maps and more

A new theory suggests the hot, harsh planet’s interior could have contained volatiles like water

Some distilleries are donating their new product to local communities in need

With no new infections reported in Hubei province in recent days, restrictions are easing up—but experts worry about possible 'second wave' of cases

Matilda McCrear was just 2 when she was captured and brought to Alabama on the "Clotilda"

Storms reveal, then hide, the ship's sand-covered remains every decade or so

Starved of resources, these hardy bacteria still eke out a living, suggesting life forms could survive in the harsh habitats on other planets

Blending marshmallows with scientific rigor, the contest offers levity during a difficult time

The "lost" apples will help restore genetic, culinary diversity to a crop North America once produced in astonishing variety

The computer program can identify canine versus human feces based on DNA sequences in samples

Canine-equid pairs can mimic each other’s facial expressions during play, which has never been seen between animals of different species

A rare bloom of microscopic organisms capable of making their own blue light has transformed several of the state’s beaches

Researchers are attempting to teach eight dogs to detect the pandemic, which could help quickly screen large numbers of people in public places

Items recovered include fossils, paintings, ancient coins, ceramics and jewelry

The actor is one of 17 celebrities slated to participate in newly announced read-alongs of the series' first book

The app features a database of some two million artworks housed at more than 120 venues

A Smithsonian folklorist examines Jewish humor in the midst of a pandemic

Graffiti artists and muralists are sending messages of hope and despair with coronavirus public art

Why not use quarantine as an opportunity to have your homeschoolers master woodworking or engine repair?

This photo shows the beautiful landscape of Capital Reef National Park with its amazing old orchards in foreground. Capital Reef has many old orchards of peaches, apples and apricots. The park lets you eat all you want if you eat it in the orchard.

Hidden behind mountains, charming village,on the edge of the cliff with waterfall. What a place to live

Using the lunar module as a lifeboat and employing techniques never before considered, the astronauts' ordeal ended triumphantly

During the Nazi occupation of France, many valuable works of art were stolen from the Jeu de Paume museum and relocated to Germany. One brave French woman kept detailed notes of the thefts

Henry Ford spent the majority of the war as a pacifist. By 1917, however, his state-of-the-art assembly line was churning out vital engine parts to feed the war machine.

A female leopard is risking life and limb by trying to steal food from another male leopard. One wrong move and the male, a third bigger than she is, could make her pay.

Yellowknife RCMP confirmed the death of the missing 22-year-old woman on Friday and charged 27-year-old Devon Larabie in connection with her death.

From bikes to vinyl records to a remote controlled airplane, Yellowknifers looking to get rid of or find second-hand treasures have a new place to look.

The remote cabin could be the difference between life and death for travellers stuck on the territory’s long winter road. It was built to remember Stella Barnaby, who would have been 55 this Saturday.

Ontario Provincial Police (OPP) have identified the person struck and killed by a train in Kenora on Wednesday as 18-year-old Tyrease Payash, of Kenora.

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“Listening to their testimonies, I’ve begun to envision their stories as a collection of clues, a series of scenes revealing the fingerprints of something—or Someone—beyond our deepest imagination,” says Chris. “The people I talked with hail from a variety of backgrounds—atheist German to Cambodian Buddhist—but the tapestries of their lives reveal the same beautiful threads, pointing unmistakably to a Designer.“

When Elizabeth May, parliamentary leader of the federal Green Party, proclaimed on Tuesday that "oil is dead," she was correct in a philosophical sense. But not in a practical, real world sense.

Before COVID-19 hit, Hemliss Eloïse Konan had plans for how she'd spend her summer in Sudbury. After finishing her first year at Laurentian University, Konan planned to stay in residence, and get a job for the summer.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.

This is a simple method for rapid preparation of recombinant adeno-associated virus (rAAV) stocks, which can be used for in vivo gene delivery. The purity of these vectors is considerably lower than that obtained by either CsCl gradient centrifugation or by combination of iodixanol gradient ultracentrifugation followed by column chromatography.

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.

This protocol describes the preparation of the shuttle vector before its introduction into bacterial artificial chromosome (BAC) host cells for BAC two-step engineering. The homology arm sequences, prepared previously, are introduced by ligation into the digested shuttle vector DNA to provide sites for recombination within the BAC clone. Crude lysates of individual bacterial transformants serve as templates in polymerase chain reaction (PCR) analysis to confirm the presence of the homology arms in the recombinant shuttle vector.

The 700-bp A homology arm (A-box) and the 700-bp B homology arm (B-box) are amplified by polymerase chain reaction (PCR) using purified bacterial artificial chromosome (BAC) DNA as template for two-step BAC engineering. The resulting A-box PCR product contains an AscI site at its 5' end (the 5' primer incorporates an AscI site, and the 3' primer does not incorporate any restriction sites). The B-box PCR product contains an XmaI site at its 3' end (the 5' primer does not incorporate any restriction sites, and the 3' primer incorporates an XmaI site). The amplification products are then digested with the appropriate restriction endonucleases to render them suitable for cloning into the shuttle vector.

In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

BIS Bulletin No 8, April 2020. Information on local labour markets and Google searches can be used to construct a measure of the vulnerability of employment in different regions of the United States to the Covid-19 shock. Regional exposure to Covid-19 varies significantly, ranging from a low of 2% to a high of 98% of total local employment. We test for the usefulness of the Covid-19 exposure measure by showing that areas with higher exposure report more Google search queries related to the pandemic and unemployment benefits.