SuperSport United winger Aubrey Modiba says a conversation with club CEO Stanley Matthews convinced him to stay despite interest from Mamelodi Sundowns.

My friend Steve Davee posted this fun project to Instructables. It's a perfect project for shut-in parents and kids to do together.

The main body parts of the bots are keyboard keycaps and Q-tips/cotton swabs. An eccentric weight (pager) motor provides the bouncy movement that makes your vibrobots go.

Dip the swabs in watercolor paints, place the little critter on some paper, and watch your little tabletop Jackson Pollockbot go to town.

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Canadians working aboard two cruise ships who weren't allowed to come to shore because of concerns about COVID-19 are finally able to return home.

gali_nette posted a photo:

China's National Health Commission reported 14 new confirmed coronavirus cases on May 9, the highest number since April 28, including the first for more than a month in the city of Wuhan where the outbreak was first detected late last year. While China had officially designated all areas of the country as low-risk last Thursday, the new cases according to data published on Sunday represent a jump from the single case reported for the day before. The new Wuhan case, the first reported in the epicentre of China's outbreak since April 3, was previously asymptomatic, according to the health commission.

Travel blogger and photographer Andy Luten drove 4,200 miles across six states to see the grounded jets, detailing the shocking state of aviation.

Although a robust inflammatory response is needed to combat infection, this response must ultimately be terminated to prevent chronic inflammation. One mechanism that terminates inflammatory signaling is the production of alternative mRNA splice forms in the Toll-like receptor (TLR) signaling pathway. Whereas most genes in the TLR pathway encode positive mediators of inflammatory signaling, several, including that encoding the MyD88 signaling adaptor, also produce alternative spliced mRNA isoforms that encode dominant-negative inhibitors of the response. Production of these negatively acting alternatively spliced isoforms is induced by stimulation with the TLR4 agonist lipopolysaccharide (LPS); thus, this alternative pre-mRNA splicing represents a negative feedback loop that terminates TLR signaling and prevents chronic inflammation. In the current study, we investigated the mechanisms regulating the LPS-induced alternative pre-mRNA splicing of the MyD88 transcript in murine macrophages. We found that 1) the induction of the alternatively spliced MyD88 form is due to alternative pre-mRNA splicing and not caused by another RNA regulatory mechanism, 2) MyD88 splicing is regulated by both the MyD88- and TRIF-dependent arms of the TLR signaling pathway, 3) MyD88 splicing is regulated by the NF-κB transcription factor, and 4) NF-κB likely regulates MyD88 alternative pre-mRNA splicing per se rather than regulating splicing indirectly by altering MyD88 transcription. We conclude that alternative splicing of MyD88 may provide a sensitive mechanism that ensures robust termination of inflammation for tissue repair and restoration of normal tissue homeostasis once an infection is controlled.

Bacterial products such as lipopolysaccharides (or endotoxin) cause systemic inflammation, resulting in a substantial global health burden. The onset, progression, and resolution of the inflammatory response to endotoxin are usually tightly controlled to avoid chronic inflammation. Members of the NF-κB family of transcription factors are key drivers of inflammation that activate sets of genes in response to inflammatory signals. Such responses are typically short-lived and can be suppressed by proteins that act post-translationally, such as the SOCS (suppressor of cytokine signaling) family. Less is known about direct transcriptional regulation of these responses, however. Here, using a combination of in vitro approaches and in vivo animal models, we show that endotoxin treatment induced expression of the well-characterized transcriptional repressor Krüppel-like factor 3 (KLF3), which, in turn, directly repressed the expression of the NF-κB family member RELA/p65. We also observed that KLF3-deficient mice were hypersensitive to endotoxin and exhibited elevated levels of circulating Ly6C+ monocytes and macrophage-derived inflammatory cytokines. These findings reveal that KLF3 is a fundamental suppressor that operates as a feedback inhibitor of RELA/p65 and may be important in facilitating the resolution of inflammation.

Antibodies are widely used as cancer therapeutics, but their current use is limited by the low number of antigens restricted to cancer cells. A receptor tyrosine kinase, receptor tyrosine kinase-like orphan receptor 2 (ROR2), is normally expressed only during embryogenesis and is tightly down-regulated in postnatal healthy tissues. However, it is up-regulated in a diverse set of hematologic and solid malignancies, thus ROR2 represents a candidate antigen for antibody-based cancer therapy. Here we describe the affinity maturation and humanization of a rabbit mAb that binds human and mouse ROR2 but not human ROR1 or other human cell-surface antigens. Co-crystallization of the parental rabbit mAb in complex with the human ROR2 kringle domain (hROR2-Kr) guided affinity maturation by heavy-chain complementarity-determining region 3 (HCDR3)-focused mutagenesis and selection. The affinity-matured rabbit mAb was then humanized by complementarity-determining region (CDR) grafting and framework fine tuning and again co-crystallized with hROR2-Kr. We show that the affinity-matured and humanized mAb retains strong affinity and specificity to ROR2 and, following conversion to a T cell–engaging bispecific antibody, has potent cytotoxicity toward ROR2-expressing cells. We anticipate that this humanized affinity-matured mAb will find application for antibody-based cancer therapy of ROR2-expressing neoplasms.

Prevention of aberrant cutaneous wound repair and appropriate regeneration of an intact and functional integument require the coordinated timing of fibroblast and keratinocyte migration. Here, we identified a mechanism whereby opposing cell-specific motogenic functions of a multifunctional intracellular and extracellular protein, the receptor for hyaluronan-mediated motility (RHAMM), coordinates fibroblast and keratinocyte migration speed and ensures appropriate timing of excisional wound closure. We found that, unlike in WT mice, in Rhamm-null mice, keratinocyte migration initiates prematurely in the excisional wounds, resulting in wounds that have re-surfaced before the formation of normal granulation tissue, leading to a defective epidermal architecture. We also noted aberrant keratinocyte and fibroblast migration in the Rhamm-null mice, indicating that RHAMM suppresses keratinocyte motility but increases fibroblast motility. This cell context–dependent effect resulted from cell-specific regulation of extracellular signal-regulated kinase 1/2 (ERK1/2) activation and expression of a RHAMM target gene encoding matrix metalloprotease 9 (MMP-9). In fibroblasts, RHAMM promoted ERK1/2 activation and MMP-9 expression, whereas in keratinocytes, RHAMM suppressed these activities. In keratinocytes, loss of RHAMM function or expression promoted epidermal growth factor receptor–regulated MMP-9 expression via ERK1/2, which resulted in cleavage of the ectodomain of the RHAMM partner protein CD44 and thereby increased keratinocyte motility. These results identify RHAMM as a key factor that integrates the timing of wound repair by controlling cell migration.

Actinobacillus pleuropneumoniae (App) is the etiological agent of acute porcine pneumonia and responsible for severe economic losses worldwide. The capsule polymer of App serotype 1 (App1) consists of [4)-GlcNAc-β(1,6)-Gal-α-1-(PO4-] repeating units that are O-acetylated at O-6 of the GlcNAc. It is a major virulence factor and was used in previous studies in the successful generation of an experimental glycoconjugate vaccine. However, the application of glycoconjugate vaccines in the animal health sector is limited, presumably because of the high costs associated with harvesting the polymer from pathogen culture. Consequently, here we exploited the capsule polymerase Cps1B of App1 as an in vitro synthesis tool and an alternative for capsule polymer provision. Cps1B consists of two catalytic domains, as well as a domain rich in tetratricopeptide repeats (TPRs). We compared the elongation mechanism of Cps1B with that of a ΔTPR truncation (Cps1B-ΔTPR). Interestingly, the product profiles displayed by Cps1B suggested processive elongation of the nascent polymer, whereas Cps1B-ΔTPR appeared to work in a more distributive manner. The dispersity of the synthesized products could be reduced by generating single-action transferases and immobilizing them on individual columns, separating the two catalytic activities. Furthermore, we identified the O-acetyltransferase Cps1D of App1 and used it to modify the polymers produced by Cps1B. Two-dimensional NMR analyses of the products revealed O-acetylation levels identical to those of polymer harvested from App1 culture supernatants. In conclusion, we have established a protocol for the pathogen-free in vitro synthesis of tailored, nature-identical App1 capsule polymers.

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

European dominance in U.S. immigration flows has decreased significantly since World War II, a result of economic, demographic, and policy trends on both sides of the Atlantic. Today, migration from European Union Member States to the United States, while small, is characterized by a substantial numbers of European scientists, professionals, and businesspeople.

Cowrie shells are a group of sea snails that range in size from small to large. These shells are famous the world over, with the most popular species of this shell known as the money cowrie or Monetaria moneta. The money cowrie are double-sided,...

Declaring it has been “pained” by the Jodian Fearon situation, the embattled Andrews Memorial Hospital (AMH) is speaking out, saying it has never been approached for or rejected efforts at registration or certification since it began operating 76...

This report finds that the 813,000 U.S. children under the age of 10 who have Black immigrant parents from Africa or the Caribbean generally fall in the middle of multiple well-being indicators, faring less well than Asian and white children but better than their native-born Black and Hispanic peers. Citizenship status, English proficiency, parental characteristics, poverty, housing, and access to social supports are examined.

Immigration from the Caribbean to the United States is a relatively recent phenomenon, beginning largely after 1965. This report provides a demographic profile of the 1.7 million Caribbean immigrants in the United States: their geographic settlement, education and workforce characteristics, earnings, modes of entry, and more.

The operator Jamaica’s two largest airports wants to scrap or delay non-essential capital projects. It forms part of a wider halt of capital projects by the Grupo Aeroportuario del Pacífico – which translate to Pacific Airport Group, or GAP –...

This practical handbook highlights policies and programs that can magnify the resources, both human and financial, that emigrants and their descendants contribute to development. It gives concrete examples of policies and programs that have been effective, and pulls out both useful lessons and common challenges associated with the topics at hand.

In 1964, Millie Small made one of only two or three trips back to her homeland, Jamaica, and it is an occasion that is engraved in singer Dennis Alcapone’s heart. He was fortunate enough to have actually seen the sensational My Boy Lollipop singer...

Immigrants have been disproportionately hit by the global economic crisis that began in 2008 and now confront a number of challenges. The report, which has a particular focus on Germany, Ireland, Spain, the United Kingdom, and United States finds that the unemployment gap between immigrant and native workers has widened in many places.

A new imaging technique may give researchers fresh insights into brain development, function, and disease

The human brain is often said to be the most complex object in the known universe, and there’s good reason to believe that it is. That lump of jelly inside your head contains at least 80 billion nerve cells, or neurons, and even more of the non-neuronal cells called glia. Between them, they form hundreds of trillions of precise synaptic connections; but they all have moveable parts, and these connections can change. Neurons can extend and retract their delicate fibres; some types of glial cells can crawl through the brain; and neurons and glia routinely work together to create new connections and eliminate old ones.

These processes begin before we are born, and occur until we die, making the brain a highly dynamic organ that undergoes continuous change throughout life. At any given moment, many millions of them are being modified in one way or another, to reshape the brain’s circuitry in response to our daily experiences. Researchers at Yale University have now developed an imaging technique that enables them to visualise the density of synapses in the living human brain, and offers a promising new way of studying how the organ develops and functions, and also how it deteriorates in various neurological and psychiatric conditions.

Related: Brain’s immune cells hyperactive in schizophrenia

Related: 3D model of a nerve terminal in atomic detail | Mo Costandi

New research provides evidence for the idea that sleep restores cellular homeostasis in the brain and helps us to forget irrelevant information

We spend one third of our lives sleeping, but we still do not know exactly why we sleep. Recent research shows that that the brain does its housekeeping while we sleep, and clears away its waste. According to another hypothesis, sleep plays the vital role of restoring the right balance of brain synapses to enhance learning, and two studies published in today’s issue of Science now provide the most direct evidence yet for this idea.

We do know that sleep is important for consolidating newly formed memories. During waking hours, we learn all kinds of new information, both consciously and unconsciously. To store it, the brain modifies large numbers of synaptic connections, making some of them stronger and larger, and it’s now thought that as we sleep other synapses are weakened or destroyed, so that the important new information is stored for later use, while irrelevant material, which could interfere with learning, is not.

Related: The Homer Simpson effect: forgetting to remember

Related: How to optimise your brain's waste disposal system

....GOING TO DO WHEN THEY BELIEVE THEY APPLY TO THEM BUT THEY HAVEN'T BEEN HOLY SPIRIT BAPTIZED? TODAY'S CHRISTIANS HAVEN'T BEEN "QUICKENED BACK TO LIFE" (EPHESIANS 2:1)  BY HIS "POWER THAT CAME UPON THEM" (ACTS 1:8) WITH HOLY SPIRIT BAPTISM (ACTS 1:5) AS THEY WERE "BORN AGAIN OF THE SPIRIT" (JOHN 3:5-7) FROM THEIR "DEAD SPIRITS" (ROMANS 5:12-14) AND WERE "RESURRECTED" (I COR 15) FROM THEIR GRAVES" (EZEKIEL 37:12-14). WHAT ARE THESE "DEAD SPIRITS" GOING TO DO? 

Letter submitted by Marc R. Rosenblum, Deputy Director, U.S. Immigration Program, at the request of the Minority Staff of the House Oversight and Government Reform Committee for the February 25, 2015 joint subcommittee hearing reviewing apprehension, detention, and release policies regarding unauthorized immigrants.

Correctional Service of Canada says an inmate, who escaped from an Edmonton facility in 2018, was arrested by police in Saskatoon on Saturday morning.

The Nova Scotia government is going ahead with its annual lottery for moose hunting licences after the latest survey found Cape Breton moose numbers have rebounded from a low of 1,300 last year.

As interest in the application of plasma medicine -- the use of low-temperature plasma created by an electrical discharge to address medical problems -- continues to grow, so does the need for research advancements proving its capabilities and potential impacts on the health care industry.

(May 1, 2020) On April 24, 2020, Singapore’s chief justice announced an extension to June 1, 2020, of the period during which the courts of Singapore would hear only essential and urgent matters in order to slow local transmission of COVID-19. On the same day, the Supreme Court, state courts, and family justice courts each […]

(May 6, 2020) On April 16, 2020, the Norwegian government launched a smartphone app to track the spread of the coronavirus in Norway. By downloading the app, users voluntarily allow the app to track their physical location and notify them if they have been in close proximity to a person who later becomes infected with […]

(May 7, 2020) On April 30, 2020, Singapore’s minister for health made an amendment to the COVID‑19 (Temporary Measures) (Control Order) Regulations 2020 to extend the expiration date of the COVID-19 control orders under the regulations to June 1, 2020. The Control Order Regulations, which had been originally issued on April 7, 2020, under the authorization […]

The singer-composer, who has collaborated with quite a few international names in music, has his plans firmly in place.

Personal Agenda with Ricky Kej: “Best thing about lockdown? My dog is really happy!”  Hindustan Times

Producer Karim Morani's daughter Zoa Morani donated her blood for plasmatherapy trials for COVID-19 treatment at Mumbai's Nair hospital today.

1. China reports first coronavirus case in Wuhan since April 3  Economic Times
2. China admits coronavirus exposed ‘shortcomings’ in healthcare system  Hindustan Times
3. "Outbreak Big Test That Revealed China's Shortcomings": Top Officer  NDTV
4. China reports 14 new coronavirus cases, high-risk area resurfaces  Zee News
5. 34 new Covid-19 cases reported in China, first in Wuhan in more than a month  The Newsopedia
6. View Full coverage on Google News

1. Happy Mother's Day: Karisma to Sushmita, a look at Bollywood's single mum's who have paved their own way  PINKVILLA
2. Mother’s Day 2020: From Aishwarya Rai to Kareena Kapoor, check out pics of these Bollywood moms and...  Hindustan Times
3. Happy Mother's Day 2020: Kareena Kapoor, Shilpa Shetty lead B-Town stars celebrate motherhood, wish mothers on this precious day | In Pics  Jagran English
4. When Aishwarya Rai Bachchan was asked to pick between Kareena Kapoor Khan & Rani Mukerji; Here’s what she said  PINKVILLA
5. Mother’s Day 2020: Single Mothers of Bollywood  News18
6. View Full coverage on Google News

1. The person who loves us tirelessly and unconditionally: Ravi Shastri, Virat Kohli wish Happy Mother's Day  India Today
2. Maa Jaisa Koi Nahi: Virat Kohli and Virender Sehwag Lead Mother's Day Wishes  News18
3. Mother’s Day: Sports stars thank their moms for unconditional love, sacrifices and prayers  The Indian Express
4. Virat Kohli, Yuvraj Singh Lead Wishes As Sports Fraternity Celebrate Mother's Day 2020  NDTVSports.com
5. Mothers Day: Laxman, Mayank, Rahane lead as cricketers extend wishes to super moms - Republic World  Republic World
6. View Full coverage on Google News

For the past few weeks, Windsor's new pandemic response therapist Dana St. Jean has been spending her time fielding phone calls and speaking with people over video chat to find ways to help them cope with the stresses of COVID-19.

On 23 January 2015, OM South Africa celebrated Pranesh Anandlal’s leadership as director since 2008 and welcomed his successor, Andrè van der Bergh.

During the Go Challenge, the team is asked to pray for an elderly woman while doing door to door ministry in Tzaneen.

After watching others be baptised, an Uzbek lady who had recently repented in the church expressed her desire to follow the others’ example.

OM Philippines meets some of the young girls caught in the rising tide of underage sex tourism.

A teenage girl's courage and boldness opens door for family´s salvation (Philippines).