Two men are charged in connection with an illegal drug distribution operation after Winnipeg police seized more than $2.2 million worth of methamphetamine, cocaine and ecstasy.

Philip Pynn is being released from federal prison for a second time, after breaching conditions landed him back behind bars last year — but it's not clear where he can live.

An OM worker from Latin America uses his carpentry skills to disciple young men in their faith and give them better employment opportunities for the future.

OM teams in five countries along the Mekong River in South East Asia are focusing on discipling local believers to see them equipped to share about Jesus with those who have never heard His name.

Equip Day is a special time to encourage the Service Team at TeenStreet (TS) to be part of God's mission. Taking the time to prepare before the teens arrive is proving to be a valuable addition to the annual TS schedule.

Much like a prehistoric pest trapped in amber, our summer plans remain in suspended animation.

The only way to solubilize many antigens for immunoprecipitation is by denaturation. This cell lysis protocol is ideally suited for this purpose to release proteins from complex structures or reveal antibody epitopes hidden within native proteins. Short linear epitopes may not be accessible to antibodies within the native tertiary and quaternary protein structures, but they become exposed upon the unraveling of proteins, exposing their secondary structure. Antibodies otherwise not suitable for the immunoprecipitation of proteins prepared under nondenaturing conditions are now able to bind these antigens of interest in cell lysates prepared under denaturing conditions. These antibodies may also work well for immunoblotting purposes when the protein target is completely denatured. Harvested cells in this protocol are washed in tris-buffered saline (TBS) before lysis in 2% sodium dodecyl sulfate (SDS)-containing Lysis buffer for 10 min at 100°C. The resulting sample is diluted 20-fold in TBS to reduce the SDS concentration to ≤0.1% before the addition of an antibody for immunoprecipitation. Addition of 2% bovine serum albumin (BSA) or 0.1% Nonidet P-40 to the TBS before an immunoprecipitation, respectively, ensures either removal of SDS from the target protein or retaining denatured proteins in solution.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

Purified RNA may need to be concentrated by precipitation for downstream applications. Precipitation of RNA with ethanol (or isopropanol) is the standard method to recover RNA from aqueous solutions.

For most immunoblots developed with chemiluminescence or with fluorochrome-based detection systems, it is possible to remove the primary and secondary antibodies from the membrane without affecting the bound antigen. This allows you to reuse the membrane for detection of another protein antigen. The blots developed with chromogenic substrates can also be stripped of antibodies and reprobed, but the bands detected in the first round of immunoblotting will remain unaffected. Stripping and reprobing of the membrane are particularly useful when the amount of sample is limited or when it is important to accurately compare the signal between two different protein antigens in the same sample. Examples of such experiments include determining the levels of a protein antigen in a series of samples relative to the loading control and comparison of the phosphorylated form to the total levels of the protein in the sample.

The BIS Board of Directors has decided to invite the central banks of Kuwait, Morocco and Vietnam to become members of the BIS. This is the first such expansion since 2011 and will take the number of members to 63.

BCBS Press release "Basel Committee issues progress report on banks' implementation of the 'Principles for effective risk data aggregation and reporting'", 29 April 2020

Some school travel groups in Cape Breton that had trips cancelled in March due to COVID-19 are still waiting to get their money back.

The COVID-19 pandemic has created a surge in nationwide shipments of specialities from legendary restaurants

A Winnipeg School Division trustee who was suspended from the school board earlier this week does not plan on fighting the decision, she told CBC News.

Winnipeg police officers who arrested a man who then went into medical distress and later died were not at fault, an investigation by Manitoba's police watchdog has found.

Manitoba municipalities will be receiving most of their operating funding from the province sooner than usual because of the COVID-19 pandemic, the province announced Friday.

The Winnipeg Airports Authority delayed a vote at Winnipeg's city council after it sent a request to move a dispute on development at Polo Park to the provincial municipal board, saying it was doing so "in the name of the government of Canada." But the government of Canada say the WAA can't speak on its behalf.

A group of Winnipeg-born hockey players connected with a sexist group chat are being either let go from their teams or investigated, after screenshots from the private chat were made public earlier this week.

Winnipeg will soon test "small cell" technology to improve cellular reception in parts of Winnipeg, ahead of a possible future transition to 5G service. 

Legal experts and leading scientists have teamed up with Creative Commons to create the Open COVID Pledge to help speed up the battle against the coronavirus pandemic. The Pledge gives broad permission to anyone to use intellectual property not otherwise accessible to the public, and generally replaces the need for any other license or royalty agreement.

Address by Mr Philip Lowe, Governor of the Reserve Bank of Australia, at the Reserve Bank of Australia, Sydney, 21 April 2020.

Speech by Mr Benjamin E Diokno, Governor of Bangko Sentral ng Pilipinas (BSP, the central bank of the Philippines), at an investor call with Standard Chartered Bank, Manila, 22 April 2020.

The National Capital Commission has reversed its decision and will now allow passersby to take photos at this year's Canadian Tulip Festival.

The National Capital Commission (NCC) says tulip admirers can "feel free" to take photos of the colourful flowers while visiting the Canadian Tulip Festival after placing signs saying photography wasn't allowed in tulip beds earlier this week.

Ice dancers Piper Gilles and Paul Poirier headline the Canadian team that will compete at this year's world figure skating championships, but Skate Canada is waiting until next month to make a final decision on several of their teammates.

For Canadian champion Roman Sadovsky and former Canadian champions Nam Nguyen and Keegan Messing, the Four Continents results will determine who takes the one spot available in the men's event at the worlds.

Canada's Joseph Phan was eighth after a highly competitive men's short program Wednesday at the world junior figure skating championships.

Quebec Health Minister Danielle McCann says the government is evaluating whether to allow the world figure skating championships to go ahead next week in Montreal.

The world figure skating championships, scheduled for March 18-22 in Montreal, have been cancelled because of the spread of COVID-19.

With this year's worlds cancelled because of the COVID-19 pandemic, Rob Pizzo looks at the last time the event didn't happen.

Govt hikes borrowing limit by over 50% to Rs 12 trn as virus grips economy  Business StandardRaghuram Rajan says monetisation neither a game changer nor catastrophe  LivemintBank credit up 6.74% to Rs 102.69 lakh crore; deposits ris...

Wellington OPP say three people made off with electric toothbrushes worth more than $700 from a pharmacy in Erin, Ont., on Wednesday.

Last month, the National Arts Centre launched #CanadaPerforms, an initiative that pays musicians and writers to perform from their living rooms. Poet Zoey Roy gave an intimate performance from her living room in Kingston. 

The San Francisco 49ers acquired one Pro Bowl left tackle and said goodbye to another as they traded for LT Trent Williams.

The 2020 NFL Draft was the most watched ever, with more than 55 million U.S. viewers tuning in during the unique three-day event.

Dentists in B.C. are trying to figure out how they might reopen by May 19 as the province begins to loosen restrictions after flattening the infection curve during the COVID-19 pandemic.

A B.C. fentanyl trafficker with a history of ignoring court orders is seeking “exceptional parole” because of COVID-19, despite the fact he’s only one year into an eight-year sentence.

There's a wonderfully quirky — and little known — information archive in downtown Winnipeg that predates Google and probably has more hidden secrets than the search engine giant.

An idea that began when a classicist went to a brewery to sip beers and ponder the history of hops has brought to life an ancient ale.

The string of pratfalls that was the comedic highlight of this year's New Waterford Coal Bowl Classic in Nova Scotia will be seen by millions of TV viewers.

OM Finland extends love and fellowship to immigrant mothers through friendship groups, open conversation and learning together.

Madagascar is putting its self-proclaimed, plant-based "cure" for COVID-19 on sale and several countries in Africa have already put in orders for purchase, despite warnings from the World Health Organization that its efficacy is unproven.

A proposed class action lawsuit has been filed in B.C. Supreme Court which, if successful, could mean every ICBC-insured motorist and crash victim will be in line for a share of almost $1 billion. 

Municipalities across the province are re-evaluating their financial situations as COVID-19 continues to keep municipal facilities closed and has put other revenue streams on hold. 

Nearly a quarter-million more Albertans lost work in April as the economic shutdown due to COVID-19 continued, with young workers — particularly young women — being disproportionately affected.

The world women's curling championship in Prince George, B.C. has been cancelled because of the outbreak of COVID-19.

The University of Alberta Pandas defeated the UNB Reds 10-2 in the U Sports women's curling final, while the Laurier Golden Hawks topped the Dalhousie Tigers 8-5 for the men's curling title in Portage la Prairie, Manitoba on Sunday.