Victor Oladipo was rocking and the Pacers were rolling before NBA shutdown, leaving President Kevin Pritchard, team hopeful about possible resumption.

Pacers President Kevin Pritchard discusses the health of his team and how they're staying prepared for the season to resume.

Oladipo: 'I can look at it like I was just getting my rhythm back or as an opportunity to rest and ... continue building strength in my tendon.'

The Indiana Pacers would have started the 2020 NBA playoffs this weekend, but these memories will have to do for now.

The Pacers dealt the No. 2 pick in the 1984 draft for one season of Tom Owens

Source: The Pacers will listen to the government and follow its lead before bringing players back

The punching bag in The Last Dance documentary about the final season of the champion Bulls is GM Jerry Krause, who got his start from Slick Leonard.

The Pacers didn't have their starting point guard for the last three games before the NBA went on hiatus, but he's able to play if/when it restarts.

The Adidas move was expected; each side alleges the other is at fault

Turner: 'It was a rough path for a couple of weeks'

Pacers Gaming kicks off NBA 2K League season on ESPN2

Indiana Pacers TV announcers Chris Denari and Quinn Buckner are among those up for honors

Pacers coach Nate McMillan is hoping to be in the final days of a stay-at-home order, and he's letting players know they need to be ready to return.

Indiana Pacers are coming off a second straight season with 48 regular-season wins and a first-round playoff loss

'Last Dance' brings back memories of tough 90's NBA for Pacers coach Nate McMillan

Mike Storen, the Pacers' first general manager, former ABA commissioner and the father of ESPN broadcaster Hannah Storm, died Thursday. He was 84.

The Pacers dealt the No. 2 pick in the 1984 draft for one season of Tom Owens

The Pacers didn't have their starting point guard for the last three games before the NBA went on hiatus, but he's able to play if/when it restarts.

Source: The Pacers will listen to the government and follow its lead before bringing players back

The punching bag in The Last Dance documentary about the final season of the champion Bulls is GM Jerry Krause, who got his start from Slick Leonard.

Turner: 'It was a rough path for a couple of weeks'

The Adidas move was expected; each side alleges the other is at fault

Indiana Pacers are coming off a second straight season with 48 regular-season wins and a first-round playoff loss

Pacers coach Nate McMillan is hoping to be in the final days of a stay-at-home order, and he's letting players know they need to be ready to return.

Pacers Gaming kicks off NBA 2K League season on ESPN2

Indiana Pacers TV announcers Chris Denari and Quinn Buckner are among those up for honors

'Last Dance' brings back memories of tough 90's NBA for Pacers coach Nate McMillan

Mike Storen, the Pacers' first general manager, former ABA commissioner and the father of ESPN broadcaster Hannah Storm, died Thursday. He was 84.

Pacers Gaming kicks off NBA 2K League season on ESPN2

Indiana Pacers TV announcers Chris Denari and Quinn Buckner are among those up for honors

'Last Dance' brings back memories of tough 90's NBA for Pacers coach Nate McMillan

Mike Storen, the Pacers' first general manager, former ABA commissioner and the father of ESPN broadcaster Hannah Storm, died Thursday. He was 84.

US space agency satellites follow the melting trends in Antarctica and Greenland over 16 years.

The actor is well known for his daring stunts, but this takes things to another level.

Is Sir Richard Branson's space tourism venture, Virgin Galactic, looking for a European base?

Other Space Synopsis Other Space is a Science Fiction comedy television series produced by Yahoo that premiered in April of 2015. Created by Paul Feig (Freaks and Geeks) Other Space is set in the early 22nd century and follows the story of the inexperienced crew aboard UMP Cruiser on a routine space exploratory mission. In […]

February 20, 2020 (New York, NY) –For the first time, eMarketer is estimating the number of people who watch Twitch on a regular basis in its latest forecast.  Twitch hosts streaming […]

The empty space next to Winnipeg City Hall that once housed restaurants could be turned into a place to help exploited women.

The CRISPR/Cas9 nucleases have been widely applied for genome editing in various organisms. Cas9 nucleases complexed with a guide RNA (Cas9–gRNA) find their targets by scanning and interrogating the genomic DNA for sequences complementary to the gRNA. Recognition of the DNA target sequence requires a short protospacer adjacent motif (PAM) located outside this sequence. Given that the efficiency of target location may depend on the strength of interactions that promote target recognition, here we sought to compare affinities of different Cas9 nucleases for their cognate PAM sequences. To this end, we measured affinities of Cas9 nucleases from Streptococcus pyogenes, Staphylococcus aureus, and Francisella novicida complexed with guide RNAs (gRNAs) (SpCas9–gRNA, SaCas9–gRNA, and FnCas9–gRNA, respectively) and of three engineered SpCas9–gRNA variants with altered PAM specificities for short, PAM-containing DNA probes. We used a “beacon” assay that measures the relative affinities of DNA probes by determining their ability to competitively affect the rate of Cas9–gRNA binding to fluorescently labeled target DNA derivatives called “Cas9 beacons.” We observed significant differences in the affinities for cognate PAM sequences among the studied Cas9 enzymes. The relative affinities of SpCas9–gRNA and its engineered variants for canonical and suboptimal PAMs correlated with previous findings on the efficiency of these PAM sequences in genome editing. These findings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome-editing efficiency.

Members Event Webinar

Carmen Gonsalves, Head, International Cyber Policy, Ministry of Foreign Affairs, Netherlands
Suzanne Spaulding, Senior Adviser for Homeland Security, Center for Strategic and International Studies
Chair: Joyce Hakmeh, Senior Research Fellow, International Security Programme and Co-Editor, Journal of Cyber Policy, Chatham House

7 May 2020 , Volume 96, Number 3

Members Event Webinar

Carmen Gonsalves, Head, International Cyber Policy, Ministry of Foreign Affairs, Netherlands
Suzanne Spaulding, Senior Adviser for Homeland Security, Center for Strategic and International Studies
Chair: Joyce Hakmeh, Senior Research Fellow, International Security Programme and Co-Editor, Journal of Cyber Policy, Chatham House

Have you ever come across a section of a book where it felt like everything happened at breakneck speed, and you could only just barely read fast enough to keep up? Or have you seen the opposite, where it’s all nice and slow and mellow, and where you’re able to really take your time and enjoy the beauty of the words?

That’s the kind of thing I’ll be musing on today. Prose and pacing. Time and reading.

Do note, this is not about how to pace your story, that’s an entirely different topic.

The Basics

Most writers will at one point or another have heard that a full stop is a signal for the reader to breathe. The shorter the sentences are, the quicker the breathing becomes, like when you’re excited. With longer sentences, the breaths grow longer, and deeper, and you calm down.

And when you write really long sentences and don’t include any commas or other forms of punctuation your reader might just run out of breath and begin to feel a little panicked.

There’s no ideal sentence length to strive for – rather the opposite.

Continue reading Pace Your Prose — Three Thoughts on Timing at Mythic Scribes.

Members Event Webinar

Carmen Gonsalves, Head, International Cyber Policy, Ministry of Foreign Affairs, Netherlands
Suzanne Spaulding, Senior Adviser for Homeland Security, Center for Strategic and International Studies
Chair: Joyce Hakmeh, Senior Research Fellow, International Security Programme and Co-Editor, Journal of Cyber Policy, Chatham House

7 May 2020 , Volume 96, Number 3

The CRISPR/Cas9 nucleases have been widely applied for genome editing in various organisms. Cas9 nucleases complexed with a guide RNA (Cas9–gRNA) find their targets by scanning and interrogating the genomic DNA for sequences complementary to the gRNA. Recognition of the DNA target sequence requires a short protospacer adjacent motif (PAM) located outside this sequence. Given that the efficiency of target location may depend on the strength of interactions that promote target recognition, here we sought to compare affinities of different Cas9 nucleases for their cognate PAM sequences. To this end, we measured affinities of Cas9 nucleases from Streptococcus pyogenes, Staphylococcus aureus, and Francisella novicida complexed with guide RNAs (gRNAs) (SpCas9–gRNA, SaCas9–gRNA, and FnCas9–gRNA, respectively) and of three engineered SpCas9–gRNA variants with altered PAM specificities for short, PAM-containing DNA probes. We used a “beacon” assay that measures the relative affinities of DNA probes by determining their ability to competitively affect the rate of Cas9–gRNA binding to fluorescently labeled target DNA derivatives called “Cas9 beacons.” We observed significant differences in the affinities for cognate PAM sequences among the studied Cas9 enzymes. The relative affinities of SpCas9–gRNA and its engineered variants for canonical and suboptimal PAMs correlated with previous findings on the efficiency of these PAM sequences in genome editing. These findings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome-editing efficiency.