Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v) %.

Methods and devices for separating fluid-suspended plant somatic embryos and embryogenic tissue based on differences in their fluid drag properties are disclosed. Deposition method and device for depositing plant somatic embryos into embryo receiver comprising growth substrate by means of a fluid jet is disclosed. An automated system for processing plant somatic embryos from the bioreactor to the growth substrate is also disclosed.

Methods and compositions for targeted delivery of biotherapeutics are provided. The compositions comprise bile-sensitive St. thermophilus bacteria modified to release a biotherapeutic agent following bile exposure. Biotherapeutic agents released by the St. thermophilus bacteria disclosed herein include AQ and AQR rich peptides. Methods of the invention comprise administering to a subject a St. thermophilus bacterium modified to release a biotherapeutic agent following bile exposure. Administration of the St. thermophilus bacterium promotes a desired therapeutic response. The bacterium may be modified to express and release AQ or AQR rich peptides which subsequently inhibit cellular apoptosis or reduce mucosal damage. Thus, methods of the invention find use in treating or preventing a variety of gastrointestinal disorders including C. difficile infection and antibiotic-associated diarrhea.

A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams.

The invention provides an improved design for the construction of extensible nucleic acid-based, ligand-controlled regulatory systems, and the nucleic acid regulatory systems resulting therefrom. The invention contemplates improving the design of the switches (ligand-controlled regulatory systems) through the design of an information transmission domain (ITD). The improved ITD eliminates free-floating ends of the switching and the competing strands, and localizes competitive hybridization events to a contiguous strand of competing and switching strands in a strand-displacement mechanism-based switch, thereby improving the kinetics of strand-displacement. The improved regulatory systems have many uses in various biological systems, including gene expression control or ligand-concentration sensing.

Methods and agents for reducing a level of an acetylated Tau polypeptide in a cell are provided. Methods for treating a tauopathy in an individual are also provided. Also provided is a method for diagnosing a cognitive impairment disorder in an individual. Methods for identifying an agent suitable for treating a tauopathy are also provided.

Disclosed are new members of a class of non-lipid small molecule inhibitors which interfere with the interaction between phosphoinositol-3,4,5-triphosphate (PIP3) and pleckstrin homology (PH) domains. These molecules target a broad range of PIP3-dependent signaling events in vitro and exert significant anti-tumor activity in vivo, with improved activity and selectivity toward particular PH domains. The small molecule inhibitors of the invention can be used alone or together with tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) or other cancer medicament to treat cancer. Small molecule inhibitors of the invention act synergistically in combination with TRAIL and with other Akt inhibitors in treating cancer. Pharmaceutical compositions and methods for treating cancer are provided.

The invention discloses novel morphology shifting micelles and amphiphilic coated metal nanofibers. Methods of using and making the same are also disclosed.

The invention is directed to a single-step method for rapidly and efficiently preparing protein-polymer conjugates, including an insulin-polymer conjugate. According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradable polymer.

Devices and methods are provided for reducing matrix effects in protein precipitated bioanalytical samples comprising: a support, and a sorbent associated with the support capable of binding matrix interfering agents present in the bioanalytical sample, wherein the device further comprises filtering means for removing precipitated protein particles. The filtering means is a size exclusion filter or a polymeric or inorganic monolith having a maximum pore size less than or equal to the diameter of the particles to be removed from the sample, and can be integral with the sorbent or associated with the sorbent. The sorbent is characterized by sufficient selectivity between the matrix interfering agents and analytes of interest to provide retention of the matrix interfering agents while providing elution of the analytes of interest (e.g., a reversed phase or a polar modified reversed phase). Typical devices incorporating these features include luer syringe filters, individual filter cartridges, multiwell plates, pipette tips, or inline columns for multiple or single use.

Described herein are techniques for assembling a polynucleotide encoding a transcription activator-like effector nucleases (TALEN). The techniques ligate and digest necessary modules for a TALEN assembly in one reactor or system. Methods and Kits for generating a TALEN are also described.

The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to northern leaf blight. Maize plants generated by the methods of the invention are also a feature of the invention.

Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering agronomic characteristics of plants under nitrogen limiting conditions, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes an LNT1 polypeptide.

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

The invention relates to genes coding for TCP family transcription factors and having a biological role in the development of axillary buds and branch growth. Furthermore, the invention relates to the promoters of the transcription of said genes, to the genetic constructs containing same and to the uses thereof, including the use of agents that modulate the expression of these genes in order to modify plant architecture.

The present invention provides an inbred corn line designated FX6815, methods for producing a corn plant by crossing plants of the inbred line FX6815 with plants of another corn plant. The invention further encompasses all parts of inbred corn line FX6815, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line FX6815, and plants produced according to these methods.

The present invention provides an inbred corn line designated FF7354, methods for producing a corn plant by crossing plants of the inbred line FF7354 with plants of another corn plant. The invention further encompasses all parts of inbred corn line FF7354, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line FF7354, and plants produced according to these methods.

The present invention relates to a composition useful for the diagnosis of diseases associated with aberrant expression of the genes encoding the secreted proteins Futrin 1, 2, 3 and/or 4(=R-Spondin 2, 3, 1 and 4, respectively), e.g. in connection with tumors or diseases of the muscle, kidneys or bones. The present invention also relates to a pharmaceutical composition containing a compound which is capable of modifying (a) the expression of the gene encoding Futrin 1, 2, 3 and/or 4 or (b) the activity of the Futrin 1, 2, 3 and/or 4 protein.

The present invention comprises methods and systems for manipulation of media and particles, whether inert materials or biomaterials, such as cells in suspension cell culture. The methods and systems comprise use of an apparatus comprising a rotating chamber wherein the actions of the combined forces fluid flow force and centrifugal force form a fluidized bed within the rotating chamber.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. Amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. The formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample.

In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.

The invention relates to malodor controlling bacteria and related methods and compositions for the control and prevention of malodor.

The present invention relates, in general, to gene expression and, in particular, to a method of inhibiting the expression of a target gene and to constructs suitable for use in such a method.

[Problem to be Solved] The importance of sugar chains having α2,3- or α2,6-linked sialic acid at their non-reducing ends is known. Industrial production has been demanded for these sugar chain compounds. Particularly, the production of glycoprotein drugs or the like inevitably requires producing in quantity sugar chains having homogeneous structures by controlling the linking pattern (α2,6-linkage or α2,3-linkage) of sialic acid. Particularly, a triantennary or tetraantennary N-type complex sugar chain having sialic acid at each of all non-reducing ends is generally considered difficult to chemically synthesize. There has been no report disclosing that such a sugar chain was chemically synthesized. Furthermore, these sugar chains are also difficult to efficiently prepare enzymatically.[Solution]The present inventors have newly found the activity of sialyltransferase of degrading sialic acid on a reaction product in the presence of CMP and also found that formed CMP can be degraded enzymatically to thereby efficiently produce a sialic acid-containing sugar chain. The present inventors have further found that even a tetraantennary N-type sugar chain having four α2,6-linked sialic acid molecules, which has previously been difficult to synthesize, can be prepared at high yields by one-pot synthesis comprising the elongation reaction of a biantennary sugar chain used as a starting material without performing purification after each enzymatic reaction.

A stylish ready-to-assemble chaise lounge and sofa which may be easily and quickly assembled by a consumer, is provided. The invention is a kit from which either a stylish chaise lounge or a sofa may be easily assembled by a consumer using a single Allen wrench. The kits, both chaise and sofa, comprise a seat base, a long side seatback and a short side seatback, feet, fasteners and cushions. With regard to the chaise, the long and short side chaise seatbacks have rectangular lower portions with upper portions featuring a flowing curved “waterfall” seating surface which improves the comfort of a user leaning back against the seatback.

A safety bow assembly for a child seat comprises: a bow body that has opposite first and second ends; first and second connecting parts that are connected to the child seat, the first connecting part defining an accommodating space and having a side wall; and an engaging lever that is connected movably to the side wall, that is formed with an engaging element facing the accommodating space, and that is movable relative to the side wall between a first position, in which the engaging element is adapted to engage releasably a coupling part of the child seat, and a second position, in which the engaging element is adapted to be disengaged from the coupling part.

A folding chair capable of being moved between a folded position and an unfolded position, includes: two units, each of which includes first and second tubes hinged together via a hinge pin; and a canvas sheet, where each assembly of two tubes includes a rigid part at the hinge pin thereof, said rigid part being arranged between two tubes and having: a groove for receiving a tube of the assembly without any degree of freedom; first and second cradles arranged on either side of the hinge pin, such that when the chair is in the unfolded position, the first cradle bears against the upper section of the other tube and the second cradle bears against the lower section of the other tube.

A chair including a dynamically-flexible back and seat having a shape that adjusts to the movements of a user seated in the chair to enhance user comfort. Each of the back and seat has an air gap formed therewithin and a resilient frame or liner with a spring memory. The resilient liner is adapted to flex in response to compressive forces that are generated as the user slides his body back or from side-to-side in the chair. Accordingly, some of the chair back and some of the chair seat move into respective air gaps so that the shapes of the back and seat change to conform to the movements of the user. The air gaps also establish air flow ventilation channels which run laterally through the back and seat of the chair.

A premium class passenger suite that includes a main seat positioned in the suite together with separate bed. The bed has a flexible mattress of predetermined dimensions and is movable between a stowed position to one side of the main seat and a deployed position above and separate from the main seat. A drive apparatus is provided for driving the bed between the stowed and deployed positions. The main seat is configured to be movable between a seating position when the bed is stowed, and a stowed position with a lowered seat back when the bed is deployed for use.

An infant rocking seat includes a base; a track provided on the base having a first arc-shaped portion and a second arc-shaped portion meeting at a crest; a carriage having a body portion, a first pair of wheels positioned at a first end of the body portion, and a second pair of wheels positioned at a second end of the body portion; and a drive mechanism configured to move the carriage along the track. The carriage is positioned within a central portion of the base and is configured to ride along the track. A distance between the first pair of wheels and the second pair of wheels is less than a distance between centers of curvature of the first arc-shaped portion and the second arc-shaped portion.

A fitting for a vehicle seat is disclosed, in particular for a motor vehicle seat. First and second fitting parts are geared with one another via a meshing gearwheel and gear rim. A peripheral eccentric is driven by a drive element for driving a relative rolling movement of the gearwheel and the gear rim, and the first fitting part receives the eccentric which is supported on the second fitting part. A blocking element blocks the eccentric in the non-driven state of the fitting via toothing on the first fitting part, and releases the eccentric when driven. The gear rim is formed on the first fitting part and the gearwheel is formed on the second fitting part.

In a fitting for a vehicle seat, a catch assembly has a pivot axis, and the catch assembly has a catch and a lever which can be pivoted relative to one another about an axis spaced from the pivot axis. The catch has a hook-like contour and in the pivoted-out position of the backrest engages with a catch locking element for locking in a punctiform or linear contact region. The catch cooperates with a spring. The lever is mounted pivotably about the pivot axis and the lever rotatably mounts the catch. In the unloaded pivoted-out position, the perpendicular in the contact region runs on one side of the pivot axis or through the pivot axis. When a return pivoting force is imposed on the backrest in the pivoted-out position, the perpendicular in the contact region runs on the opposing side of or outside of the pivot axis.

A seat cushion, in particular for an aircraft seat, the seat cushion comprising a seat part having a receiving surface adapted to receive a person and a reinforcing part supporting the seat part, wherein at least the reinforcing part contains expanded polypropylene (EPP), preferably comprising fire retardant properties. The invention further relates to a method for manufacturing such a seat cushion, a seat comprising such a seat cushion and a vehicle comprising such a seat.

An operating unit (1) for installation in an aircraft cabin comprises an operating unit front side (10) and an operating unit rear side (20), at least one operating element (12, 14) attached in the region of the operating unit front side (10) and accessible to a user, and a holding device (40) for attaching the operating unit (1) to a monument (30) accommodating the operating unit (1) in its state mounted in the aircraft cabin. The holding device (40) is designed to attach the operating unit (1) in its state mounted in the aircraft cabin to the monument (30), in a first position or in a second position, as desired, the operating element (12, 14) in the second position of the operating unit (1) being situated in a spatial position which is lowered with respect to the spatial position of the operating element (12, 14) in the first position of the operating unit (1) and/or is displaced in a direction parallel to an imaginary straight line connecting the operating unit rear side (20) to the operating unit front side (10).

A method of making a vehicle seat assembly comprising providing a cushion having an “A” surface, a “B” surface, a central portion, and two bolster areas, with each bolster area being adjacent the central portion, the cushion having a plurality of intrusions extending from the “A” surface towards the “B” surface to form a hardness gradient between at least one of the bolster areas and the central portion of between 8% to 25%, securing the cushion to a frame, and covering cushion with a trim member.

A height adjustable platform and method include a ratchet platform having a platform upper assembly with a platform and a ratchet. The ratchet includes a platform riser tube and a ratchet rail secured to the platform riser tube. The ratchet platform also includes a platform lower assembly having a support column and a platform base. The support column is secured to the platform base. An end of the ratchet is proximate the platform and an opposing end of the ratchet is disposed within an interior of the support column. The platform upper assembly actuator includes a pedal sub assembly and an upper linkage. The upper linkage is attached to the pedal sub assembly. Actuation of the pedal sub assembly actuates the upper linkage. Actuation of the upper linkage actuates the ratchet to adjust the height of the ratchet platform. The ratchet platform also includes an energy attenuation system.

A seating unit that includes a linkage mechanism adapted to move the seating unit between seat-lift, closed, extended, reclined, and seat-lift positions is provided. The linkage mechanism includes a seat-mounting plate mounted to a footrest assembly, a base plate fixedly mounted to a lift assembly, a back-mounting link rotatably coupled to the seat-mounting plate, a seat-adjustment assembly with a bellcrank, and a linear actuator for automating adjustment of the linkage mechanism. In operation, a stroke in a first phase of the linear actuator generates a force on the bellcrank that translates the seat-mounting plate rearward in a consistent angle of inclination and rotates the back-mounting link from a reclined to an upright orientation. A stroke in a second phase acts to collapse the footrest assembly. A stroke in a third phase causes the lift assembly to raise and tilt the seating unit, thereby accommodating egress and ingress of an occupant.

A wire harness for a vehicle seating assembly includes a body portion having a plurality of engagement hooks adapted for engagement with a vehicle seat cushion pan. A latticework of interconnected lateral support members are bound by a peripheral frame.

More specifically, the crash indication system for a child safety seat includes a seat bottom. A base is provided for supporting the seat bottom, which is slidably connected to the bases so that the seat body is movable relative to the base, between a resting position and a ride down position, a position due to a change in environmental forces. The seat body is adjustably affixed to a carriage to set a recline position. An indicator member is attached to the carriage and moves therewith to visually track and monitor the movement of the seat. When ride down of the seat occurs due to a change in forces to the seat, the indicator member moves with the sliding carriage to reflect a ride down position of the seat bottom. A ratchet connection is provided to prevent the carriage from going back to its resting position.

There is provided a moveable power seat slide device assembly comprising a movable seat cushion and seat rail including upper rails secured to the seat cushion and lower rails secured to the body of the golf cart, a motor for driving each movable seat and a current control circuit. The prostate golf cart seat protects the prostate area from continuous bouncing. The seat has two 8 inch stationary end cushions. The center would consist of two 8 inch cushions moving along a rail which allows riders to automatically close the 7 inch seat gap to 3 inches from the stationary end cushion. The center air space between each rider and seat would have a depth 1½ inches.

A vehicle seating assembly includes a seatpan coupled with a track assembly that has a rail and a slide. The vehicle seating assembly also includes a cushion assembly that has a movable cushion and a base cushion with a slot. The movable cushion is slidable between a forward position and a rearward position on the seatpan. In addition, the vehicle seating assembly includes a coverstock with a first portion that is disposed over a movable cushion. The coverstock also has a second portion that is disposed between the seatpan and the cushion assembly. The second portion of the coverstock is withdrawn through the slot when the movable cushion moves to the forward position. A gap is defined by the area between the base cushion and the movable cushion in the forward position. The gap is concealed by the coverstock when the movable cushion is in the forward position.

With a fitting for a vehicle seat, in particular for a motor vehicle seat, having a first fitting part and a second fitting part, which are rotatable relative to one another and are in geared connection with each other, an enclosing ring, which axially holds together the first fitting part and the second fitting part, forming a disc-shaped unit, and which is fixed to the first fitting part and engages over the second fitting part radially outside, and a third fitting part which is pivotably mounted relative to the first fitting part and which can be locked by means of a toothed pawl with a latching element which is provided on the first fitting part, the toothed latching element is formed on the first fitting part.

A latch plate for a lock device is proposed which comprises a latch plate proper and a film that covers the latch plate proper. The latch plate proper includes upper and lower finger portions between which a striker catching recess is defined. An upper surface of the lower finger portion includes a depressed main portion located near the bottom of the striker catching recess, a raised leading end portion and a stepped portion provided between the depressed main portion and the raised leading end portion. The film covers the latch plate proper except the raised leading end portion. The film covers the depressed main portion of the upper surface in such a manner that an outer surface of the film is flush with the stepped portion. When the film is depressed by the striker, the stepped portion enhances a function is to stop a movement of the striker toward a mouth of the striker catching recess.

A mechanism for vertically adjusting a component on a shaft has a collet sleeve riding on the shaft. A spiral track of the component's hub receives a radially outwardly projecting pin on the collet sleeve. The outer surface of the collet sleeve and the inner surface of the hub are tapered so that the hub can rotated one direction to advance the hub on the collet sleeve and compress the collet sleeve onto the shaft to frictionally lock the collet sleeve to the shaft. Because the hub is joined to the collet sleeve by the pin, this also locks the vertical position of the component. Conversely, the hub can be oppositely rotated to release the compression of the collet sleeve so that the collet sleeve, and therefore the vertical component, is slidable on the shaft.

Embodiments of the present invention include a vehicle seat having a frame member and a reinforcing member for reinforcing the frame member. The frame member has an elongated portion. The elongated portion is formed of resin and preferably has a flat-plate-like or bar-like configuration. The elongated portion includes a first portion, a front second portion and a rear second portion. The first portion may be arranged in a longitudinal manner with respect to the vehicle seat and extends vertically. The front second portion extends in the lateral direction of the vehicle seat from a front end of the first portion. The rear second portion extends in the lateral direction of the vehicle seat from a rear end of the first portion. The elongated portion is configured so that a neutral axis is positioned between the front and rear second portions.

A seat back frame includes: a first side member and a second side member each formed into an elongated shape along an up-and-down direction of a seat back so as to support each side portion of the seat back; and a belt guide portion on which tension of a seat belt is applied when the seat belt is pulled, the belt guide portion being formed in an upper portion of the first side member and guiding the seat belt having the one end retained by a seat belt retaining member. The first side member is formed of a sheet of steel plate and includes: a side member side wall portion formed in parallel to a direction of application of the tension of the seat belt on the belt guide portion and formed continuously along a longitudinal direction of the first side member; a side member front wall portion formed on a front side of the vehicle with respect to the side member side wall portion; and a side member rear wall portion formed on a rear side of the vehicle with respect to the side member side wall portion, the first side member having an open cross-sectional shape.

A cushion element for a vehicle seat or headrest comprises a non-woven layer and a fiber composite material. An insert material is arranged between the non-woven layer and the fiber composite material, and has different compression properties than the fiber composite material. The fiber composite material forms together with the insert material and the non-woven layer a one-piece material composite.

An integrated tuner circuit has an arbitrary IF (intermediate frequency) output. The tuner includes an integrated circuit with a fixed-frequency control loop and a matched external variable capacitance Ct, to achieve tracking of a tuned LC band-pass filter with an arbitrary oscillator.

The present invention provides an apparatus for enhancing Q factor of an inductor. The apparatus includes a negative resistance generator coupled to the inductor for providing a negative resistance, and a bias circuit coupled to the negative resistance generator for biasing the negative resistance generator.