Greenery is sprouting from scorched tree trunks as the forests regrow their canopies

The suspects were found drunk and hiding under a tarpaulin, reportedly in possession of small stones from the fire-ravaged cathedral

Thieves pilfered "The Parsonage Garden at Nuenen in Spring 1884" from the Singer Laren in the early hours of Monday morning

Audible's new service is aimed at school-age children participating in distance learning but features selections likely to appeal to all

Though not as plentiful as the Perseids in summer, the Lyrids can serve up some serious fireballs

The two NASA astronauts will lift off from historic launch pad 39A, used for the Apollo and space shuttle missions

The city's residents sorted waste materials for reuse in future projects, according to new research

The story behind the image, and its removal, led to mixed reactions from the public, including native communities

The London institution's online offerings include 280,000 newly added Creative Commons images

The simultaneous mating calls of the male pug-nosed tree frog confuses bats but not female frogs

The app features a database of some two million artworks housed at more than 120 venues

Narrated by Smithsonian Distinguished Scholar Richard Kurin, the 24-part video series blends history with modern mainstays

How do you select one image to represent half a century of photography and art? You don’t

Tulips, cherry blossoms and orchids supply a ray of hope during self-isolation

A Smithsonian folklorist examines Jewish humor in the midst of a pandemic

These virtual versions of classic and lesser-known games are ideal for social distancing

These paintings, sculptures and illustrations honor the bonds of motherhood

Browse the collections for artworks with a CC0 license as part of the Smithsonian's Open Access Initiative,

Tour a gallery of presidential portraits, print a 3-D model of a fossil or volunteer to transcribe historical documents

From virtual museum tours to space exploration, ancient worlds and natural phenomena, this hub has you covered

Henny Sundig is a pivotal figure in the history of WWII Danish resistance. In 1943, aged just 19, she risked it all to make a daring journey in her boat, Gerda III, to rescue as many Jews as she could.

Henry Ford spent the majority of the war as a pacifist. By 1917, however, his state-of-the-art assembly line was churning out vital engine parts to feed the war machine.

The pilum, a vital weapon in Rome’s imperial ambitions, was a simple iron spear fixed with two bolts onto a heavy wooden post. It was remarkably effective against an attacker charging at high speed.

Hadrian’s Wall was a 73 mile barrier stretching from coast to coast, splitting the warlike north of Britain from the more docile south. It was the Roman Empire’s way of imposing peace in a hostile land

Veteran musher Lance Mackey has had his 21st place finish in this year's Iditarod Trail Sled Dog Race vacated because of a failed drug test, race officials announced Thursday.

Changes are coming to Thunder Bay's popular Ribfest event because of the COVID-19 outbreak, organizers said.

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This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.

In this protocol, DNA fragments are separated according to size by electrophoresis through low-melting-temperature agarose, and then recovered by melting the agarose and extracting with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 to 5.0 kb. Yields of DNA fragments outside this range are usually lower, but often are sufficient for many purposes.

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.

This protocol describes the preparation of the shuttle vector before its introduction into bacterial artificial chromosome (BAC) host cells for BAC two-step engineering. The homology arm sequences, prepared previously, are introduced by ligation into the digested shuttle vector DNA to provide sites for recombination within the BAC clone. Crude lysates of individual bacterial transformants serve as templates in polymerase chain reaction (PCR) analysis to confirm the presence of the homology arms in the recombinant shuttle vector.

The 700-bp A homology arm (A-box) and the 700-bp B homology arm (B-box) are amplified by polymerase chain reaction (PCR) using purified bacterial artificial chromosome (BAC) DNA as template for two-step BAC engineering. The resulting A-box PCR product contains an AscI site at its 5' end (the 5' primer incorporates an AscI site, and the 3' primer does not incorporate any restriction sites). The B-box PCR product contains an XmaI site at its 3' end (the 5' primer does not incorporate any restriction sites, and the 3' primer incorporates an XmaI site). The amplification products are then digested with the appropriate restriction endonucleases to render them suitable for cloning into the shuttle vector.

In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

Amid widespread sell-offs in risky asset classes, corporate bond exchange-traded funds (ETFs) traded at steep discounts to underlying asset values in March. Contributing factors were high market volatility, reduced risk-taking by dealers and investors' reaction to policy decisions. Policy interventions that improve market functioning in a given sector can have temporary yet important spillovers to other segments through portfolio rebalancing by investors.

Open for visitors, these houses model upcycling at its finest

British product designer Lucy Hughes has invented a biodegradable plastic made from fish offcuts

Basel Committee Press release "Basel Committee meets to review vulnerabilities and emerging risks, advance supervisory initiatives and promote Basel III implementationl", 27 February 2020.

CPMI Press release "How fintech can promote financial inclusion - a new report on the opportunities and challenges", 14 April 2020

A woman who lost her sister to COVID-19 at the start of the month is thanking the staff at Northwood who took "excellent" care of her in her final days. Jean Harrigan, 90, is being remembered for her caring and lively nature.

Overcoming scorching heat and little rain, experimental vineyards teach winemakers to cope with climate change

The COVID-19 pandemic has created a surge in nationwide shipments of specialities from legendary restaurants

They're not necessarily treating sick patients in hospitals, but a number of Manitoba-based scientists are working long hours and facing incredible pressure to battle the novel coronavirus from their labs and research facilities.

Health PEI plans to create a COVID-19 unit within the Prince Edward Home to be used for any long-term care resident — living in any long-term care facility on the Island — who is diagnosed with the virus.

Prosecutors who dropped charges against eight people in the Flint water scandal explained their decision in a public forum Friday night, telling frustrated, shocked and saddened residents they must look at hundreds of mobile devices and millions of documents that a previous investigative team never reviewed.

Speech by Mr Fehmi Mehmeti, Governor of the Central Bank of the Republic of Kosovo, at the press conference where the details of the measures taken by the CBK for maintaining health in the economy were given, Pristina, 3 April 2020.

Speech (via webcast)by Mr Jerome H Powell, Chair of the Board of Governors of the Federal Reserve System, at the Hutchins Center on Fiscal and Monetary Policy, The Brookings Institution, Washington DC, 9 April 2020.

Hearing of Mr François Villeroy de Galhau, Governor of the Bank of France, before the Section for the Economy and Finance of the French Economic, Social and Environmental Council, Paris, 8 April 2020.