La exsecretaria de Salud de Cali y ahora precandidata a la Alcaldía de Cali habló sobre su campaña en Caracol Radio.

La Procuraduría solicitó frenar los gastos de viaje de la primera dama

Hernando Patiño debutó de buena manera como entrenador encargado del Deportivo Cali. Su equipo empató 1-1 en su visita a Millonarios en el estadio El Campín, juego que le sirvió al equipo vallecaucano para poner fin a una racha de cuatro derrotas consecutivas, conservando las chances de llegar a los cuadrangulares finales.

Iván Velásquez se refirió a la propuesta del presidente Gustavo Petro de establecer este viernes 19 de abril como n día cívico con el propósito de promover el ahorro del agua y la energía eléctrica

Varios alcaldes, entre ellos Alejandro Eder enviaron una carta al presidente Petro para pedir que retome los bombardeos y elimine las restricciones para enfrentar a los grupos armados

En 6AM de Caracol Radio estuvo Marisol Gómez Giraldo, periodista y exconcejal de Bogotá, quien  explica a profundidad su nuevo libro donde habla de cómo es en “realidad la política”. 

En 6AM Hoy por Hoy de Caracol Radio estuvo Isabelita Mercado, Consejera de paz, viviendas y reconstrucción de la Alcaldía de Bogotá, para hablar sobre el retorno de la comunidad Embera a su territorio.

En 6AM Hoy por Hoy de Caracol Radio estuvo el senador Iván Cepeda, para hablar sobre la propuesta del Gobierno para salvar las negociaciones con el ELN y el paro armado que anunciaron en Chocó.

Juan Camilo Nariño, presidente de la Asociación Colombiana de Minería, estuvo en 6AM para ampliar la problemática sobre los efectos alrededor del decreto 044 del 2024.

Juan Camilo Nariño, presidente de la Asociación Colombiana de Minería, estuvo en 6AM para ampliar la problemática sobre los efectos alrededor del decreto 044 del 2024.

CopenhaguenEls bitllets i les monedes gairebé són una cosa del passat a Suècia, on en els últims anys els diners en efectiu pràcticament han desaparegut dels comerços i de les butxaques dels suecs. via Pocket

tataata has added a photo to the pool:

α1-antitrypsin (AAT) regulates the activity of multiple proteases in the lungs and liver. A mutant of AAT (E342K) called ATZ forms polymers that are present at only low levels in the serum and induce intracellular protein inclusions, causing lung emphysema and liver cirrhosis. An understanding of factors that can reduce the intracellular accumulation of ATZ is of great interest. We now show that calreticulin (CRT), an endoplasmic reticulum (ER) glycoprotein chaperone, promotes the secretory trafficking of ATZ, enhancing the media:cell ratio. This effect is more pronounced for ATZ than with AAT and is only partially dependent on the glycan-binding site of CRT, which is generally relevant to substrate recruitment and folding by CRT. The CRT-related chaperone calnexin does not enhance ATZ secretory trafficking, despite the higher cellular abundance of calnexin-ATZ complexes. CRT deficiency alters the distributions of ATZ-ER chaperone complexes, increasing ATZ-BiP binding and inclusion body formation and reducing ATZ interactions with components required for ER-Golgi trafficking, coincident with reduced levels of the protein transport protein Sec31A in CRT-deficient cells. These findings indicate a novel role for CRT in promoting the secretory trafficking of a protein that forms polymers and large intracellular inclusions. Inefficient secretory trafficking of ATZ in the absence of CRT is coincident with enhanced accumulation of ER-derived ATZ inclusion bodies. Further understanding of the factors that control the secretory trafficking of ATZ and their regulation by CRT could lead to new therapies for lung and liver diseases linked to AAT deficiency.

Felicia Grasso
Dec 1, 2020; 19:1986-1996
Research

Adult progenitor cell populations typically exist in a quiescent state within a controlled niche environment. However, various stresses or forms of damage can disrupt this state, which often leads to dysfunction and aging. We built a glucocorticoid (GC)-induced liver damage model of mice, found that GC stress induced liver damage, leading to consequences for progenitor cells expansion. However, the mechanisms by which niche factors cause progenitor cells proliferation are largely unknown. We demonstrate that, within the liver progenitor cells niche, Galectin-3 (Gal-3) is responsible for driving a subset of progenitor cells to break quiescence. We show that GC stress causes aging of the niche, which induces the up-regulation of Gal-3. The increased Gal-3 population increasingly interacts with the progenitor cell marker CD133, which triggers focal adhesion kinase (FAK)/AMP-activated kinase (AMPK) signaling. This results in the loss of quiescence and leads to the eventual stemness exhaustion of progenitor cells. Conversely, blocking Gal-3 with the inhibitor TD139 prevents the loss of stemness and improves liver function. These experiments identify a stress-dependent change in progenitor cell niche that directly influence liver progenitor cell quiescence and function.

Plasmodium, the malaria parasite, undergoes a complex life cycle alternating between a vertebrate host and a mosquito vector of the genus Anopheles. In red blood cells of the vertebrate host, Plasmodium multiplies asexually or differentiates into gamete precursors, the male and female gametocytes, responsible for parasite transmission. Sexual stage maturation occurs in the midgut of the mosquito vector, where male and female gametes egress from the host erythrocytes to fuse and form a zygote. Gamete egress entails the successive rupture of two membranes surrounding the parasite, the parasitophorous vacuole membrane and the erythrocyte plasma membrane. In this study, we used the rodent model parasite Plasmodium berghei to design a label-free quantitative proteomic approach aimed at identifying gender-related proteins differentially released/secreted by purified mature gametocytes when activated to form gametes. We compared the abundance of molecules secreted by wild type gametocytes of both genders with that of a transgenic line defective in male gamete maturation and egress. This enabled us to provide a comprehensive data set of egress-related molecules and their gender specificity. Using specific antibodies, we validated eleven candidate molecules, predicted as either gender-specific or common to both male and female gametocytes. All of them localize to punctuate, vesicle-like structures that relocate to cell periphery upon activation, but only three of them localize to the gametocyte-specific secretory vesicles named osmiophilic bodies. Our results confirm that the egress process involves a tightly coordinated secretory apparatus that includes different types of vesicles and may put the basis for functional studies aimed at designing novel transmission-blocking molecules.

Paramphistomosis, caused by the rumen fluke, Calicophoron daubneyi, is a parasitic infection of ruminant livestock which has seen a rapid rise in prevalence throughout Western Europe in recent years. Following ingestion of metacercariae (parasite cysts) by the mammalian host, newly-excysted juveniles (NEJs) emerge and invade the duodenal submucosa which causes significant pathology in heavy infections. The immature larvae then migrate upwards, along the gastrointestinal tract, and enter the rumen where they mature and begin to produce eggs. Despite their emergence, and sporadic outbreaks of acute disease, we know little about the molecular mechanisms used by C. daubneyi to establish infection, acquire nutrients and to avoid the host immune response. Here, transcriptome analysis of four intra-mammalian life-cycle stages, integrated with secretome analysis of the NEJ and adult parasites (responsible for acute and chronic disease respectively), revealed how the expression and secretion of selected families of virulence factors and immunomodulators are regulated in accordance with fluke development and migration. Our data show that whilst a family of cathepsins B with varying S2 sub-site residues (indicating distinct substrate specificities) are differentially secreted by NEJs and adult flukes, cathepsins L and F are secreted in low abundance by NEJs only. We found that C. daubneyi has an expanded family of aspartic peptidases, which is up-regulated in adult worms, although they are underrepresented in the secretome. The most abundant proteins in adult fluke secretions were helminth defence molecules (HDMs) that likely establish an immune environment permissive to fluke survival and/or neutralise pathogen-associated molecular patterns (PAMPs) such as bacterial lipopolysaccharide in the microbiome-rich rumen. The distinct collection of molecules secreted by C. daubneyi allowed the development of the first coproantigen-based ELISA for paramphistomosis which, importantly, did not recognise antigens from other helminths commonly found as co-infections with rumen fluke.

Volunteers will be cutting hair at a salon based inside a Cape Breton food bank. The goal is to provide clients with the same salon experience paying customers would receive.

A Port Hawkesbury woman has long imagined a Remembrance Day with her great-uncle's war medals. It will happen this year for the first time thanks to some sleuthing and the kindness of her great-uncle's foster family.

halasz gl1100 carburetor repair guide

Welcome to this week’s edition of the Steam Deck Weekly. If you missed my Gundam Breaker 4 Steam Deck review, …

Continue reading "Steam Deck Weekly: Visions of Mana and More Reviews, Reynatis Preview, Age of Mythology Retold Steam Deck Impressions, News, and More"

Une ancienne boîte de poulet St-Hubert, retrouvée par hasard dans l’entretoit d’un chalet, a été remise à la famille Martin en échange d'un don.

London-Based drug firm, AstraZeneca has entered into a new field of developing drugs using proteins secreted by the cells called as "Secretome". It has

The spruced up Tali temple pond complex serves as a portrait to the culture and history of the erstwhile rulers of Malabar

Paris : Éditions 10/18, [2014]

Tokyo : Charles E. Tuttle Company, [1964]