A host of undead enemies foolishly stand in your way as you stride through all manner of gloomy gothic environments.

When you look in the mirror you might see your mothers eyes or your father’s chin. But have you inherited more than these physical characteristics? Beyond a genetic predisposition to illness , can you inherit a tendency to be religious or obese? Are the life experiences of previous generations shaping the way you behave now?  

The gruesome remains of a shark caught on the NSW South Coast have sparked a debate about how the animal met its fate and highlighted the 'amazing healing powers' of the predators.

Central Coast Mariners midfielder Wout Brama faces a nervous wait for the A-League match review panel to ponder his horrible tackle which left Brisbane Roar forward Corey Gameiro in tears.

Пятого июня на Metal Blade состоится выпуск CD и виниловых версий альбомов MERCYFUL FATE, "Don't Break The Oath", "Melissa" и "Mercyful Fate", основой для которых были оригинальные мастер-ленты. Варианты изданий следующие:

Don't Break The Oath CD и LP:

- hardcover digisleeve "mini-LP" CD
- yellow w/ red flares vinyl
- clear w/ orange spots vinyl
- 180g black vinyl
- golden yellow marbled vinyl
- luminous orange marbled vinyl
- clear amber marbled vinyl
- opaque melon marbled vinyl
- flame splatter vinyl
- translucent red vinyl
- orange / red melt vinyl

Melissa CD и LP:

- hardcover digisleeve "mini-LP" CD
- yellow w/ black streaks vinyl
- highlighter yellow w/ red splatter vinyl
- 180g black vinyl
- opaque cherry red vinyl
- opaque golden yellow marbled vinyl
- dark green marbled vinyl
- olive green marbled vinyl
- green / blue color circle w/ red splatter vinyl
- translucent yellow vinyl
- red / black color circle w/ yellow splatter vinyl

Mercyful Fate CD и LP:

- hardcover digisleeve "mini-LP" CD
- black / white edge marbled vinyl
- clear w/ black smoke vinyl
- 180g black vinyl
- cool grey marbled vinyl
- clear ghost white vinyl
- white "blackdust" vinyl
- opaque red vinyl #Mercyful_Fate #MercyfulFate #HeavyMetal #Heavy_Metal

I turned our encounters over and over in my head, wondering if I had been too boring, unattractive, or callow. Why would he text, then drop me, then text again?

The FA Cup quarter-final draw takes place this evening but what ball number is your team? Express Sport has all the details.

SCOTTISH CUP SEMI-FINAL DRAW - Everything you need to know ahead of the draw in including date, time, TV channel and live stream.

Robert W. Mahley
Jan 1, 1999; 40:1-16
Reviews

A failure in self-tolerance leads to autoimmune destruction of pancreatic β-cells and type 1 diabetes (T1D). Low molecular weight dextran sulfate (DS) is a sulfated semi-synthetic polysaccharide with demonstrated cytoprotective and immunomodulatory properties in vitro. However, whether DS can protect pancreatic β-cells, reduce autoimmunity and ameliorate T1D is unknown. Here we report that DS, but not dextran, protects human β-cells against cytokine-mediated cytotoxicity in vitro. DS also protects mitochondrial function and glucose-stimulated insulin secretion and reduces chemokine expression in human islets in a pro-inflammatory environment. Interestingly, daily treatment with DS significantly reduces diabetes incidence in pre-diabetic non-obese diabetic (NOD) mice, and most importantly, reverses diabetes in early-onset diabetic NOD mice. DS decreases β-cell death, enhances islet heparan sulfate (HS)/heparan sulfate proteoglycan (HSPG) expression and preserves β-cell mass and plasma insulin in these mice. DS administration also increases the expression of the inhibitory co-stimulatory molecule programmed death-1 (PD-1) in T-cells, reduces interferon-+ CD4+ and CD8+ T-cells and enhances the number of FoxP3+ cells. Collectively, these studies demonstrate that the action of one single molecule, DS, on β-cell protection, extracellular matrix preservation and immunomodulation can reverse diabetes in NOD mice highlighting its therapeutic potential for the treatment of T1D.

Site-specific recombinases, such as Cre, are a widely used tool for genetic lineage tracing in the fields of developmental biology, neural science, stem cell biology, and regenerative medicine. However, nonspecific cell labeling by some genetic Cre tools remains a technical limitation of this recombination system, which has resulted in data misinterpretation and led to many controversies in the scientific community. In the past decade, to enhance the specificity and precision of genetic targeting, researchers have used two or more orthogonal recombinases simultaneously for labeling cell lineages. Here, we review the history of cell-tracing strategies and then elaborate on the working principle and application of a recently developed dual genetic lineage-tracing approach for cell fate studies. We place an emphasis on discussing the technical strengths and caveats of different methods, with the goal to develop more specific and efficient tracing technologies for cell fate mapping. Our review also provides several examples for how to use different types of DNA recombinase–mediated lineage-tracing strategies to improve the resolution of the cell fate mapping in order to probe and explore cell fate–related biological phenomena in the life sciences.

12 March 2015 , Volume 91, Number 2

Let’s have heart Let’s have heart And space ourselves Three feet apart Let’s act fast to bring this disease to its last Let’s have heart and play our part To do our best to turn the spread off Hand sanitizers Elbow coughs Masks and...

Vodou evokes a schizophrenic response among the misinformed. Reactions abound: apprehension, incredulity, and vilification. It is not that Vodou struggles for meaning. Vodou has its own particular ideal. It is primordial sense. It is very much...

Neurosciences.

Sustainable agriculture.

While President Donald Trump signed executive orders this week that could have widespread impact on immigrant communities, many in K-12 education await word on his decision on Deferred Action for Childhood Arrivals.

Vaso-occlusive episodes (VOEs) are a common complication of sickle cell disease, resulting in morbidity. Magnesium is a vasodilator and has been shown to improve red blood cell hydration. Previous small studies have suggested that treatment with magnesium may decrease VOEs.

Intravenous magnesium sulfate is well tolerated in relatively high doses but had no effect on the length of stay in hospital, pain scores, or cumulative analgesia used in children admitted with painful VOEs in sickle cell disease. (Read the full article)

Saudi Arabian equities slumped on concern the U.S. may take measures against the kingdom if it’s linked to the disappearance of Washington Post writer Jamal Khashoggi.

The world's richest cricket board has not yet pledged any donations to fight the COVID-19 pandemic. Ganguly said he will have a discussion with secretary Jay Shah to figure out the best possible option.

visit News18 Urdu for latest news, breaking news, news headlines and updates from Fatehabad on politics, sports, entertainment, cricket, crime and more.

visit News18 Urdu for latest news, breaking news, news headlines and updates from Fatehgarh Saheb on politics, sports, entertainment, cricket, crime and more.

visit News18 Urdu for latest news, breaking news, news headlines and updates from Fatehpur on politics, sports, entertainment, cricket, crime and more.

...

In the face of the continuous lockdown occasioned by the Covid-19, the Kano State Council of Ulama's have demanded to know it fate from the government on whether it will be allowed to conduct or observe the Eid congregational prayers or not which marks the end of the Muslim Ramadan fast.

The post Covid-19 Lockdown: Let’s know our fate now on Eid congregational prayers — Kano Clerics tell Govt appeared first on Vanguard News.

[Nation] Captain Mabruk Islam Sherman spoke to his wife and three children around noon, informing them of his trip to Somalia.

Commercial Court rules that St Philip's Hospital owner Frank Portelli cannot decide the hospital's fate on his own as HSBC Bank seeks repayment of €11.5 million in loans, appoints two provisional administrators

Salicinoids form a specific class of phenolic glycosides characteristic of the Salicaceae. Although salicinoids accumulate in large amounts and have been shown to be involved in plant defense, their biosynthesis is unclear. We identified two sulfated salicinoids, salicin-7-sulfate and salirepin-7-sulfate, in black cottonwood (Populus trichocarpa). Both compounds accumulated in high amounts in above-ground tissues including leaves, petioles, and stems, but were also found at lower concentrations in roots. A survey of salicin-7-sulfate and salirepin-7-sulfate in a subset of poplar (Populus sp.) and willow (Salix sp.) species revealed a broader distribution within the Salicaceae. To elucidate the formation of these compounds, we studied the sulfotransferase (SOT) gene family in P. trichocarpa (PtSOT). One of the identified genes, PtSOT1, was shown to encode an enzyme able to convert salicin and salirepin into salicin-7-sulfate and salirepin-7-sulfate, respectively. The expression of PtSOT1 in different organs of P. trichocarpa matched the accumulation of sulfated salicinoids in planta. Moreover, RNA interference-mediated knockdown of SOT1 in gray poplar (Populus x canescens) resulted in decreased levels of sulfated salicinoids in comparison to wild-type plants, indicating that SOT1 is responsible for their formation in planta. The presence of a nonfunctional SOT1 allele in black poplar (Populus nigra) was shown to correlate with the absence of salicin-7-sulfate and salirepin-7-sulfate in this species. Food choice experiments with leaves from wild-type and SOT1 knockdown trees suggest that sulfated salicinoids do not affect the feeding preference of the generalist caterpillar Lymantria dispar. A potential role of the sulfated salicinoids in sulfur storage and homeostasis is discussed.

Site-specific recombinases, such as Cre, are a widely used tool for genetic lineage tracing in the fields of developmental biology, neural science, stem cell biology, and regenerative medicine. However, nonspecific cell labeling by some genetic Cre tools remains a technical limitation of this recombination system, which has resulted in data misinterpretation and led to many controversies in the scientific community. In the past decade, to enhance the specificity and precision of genetic targeting, researchers have used two or more orthogonal recombinases simultaneously for labeling cell lineages. Here, we review the history of cell-tracing strategies and then elaborate on the working principle and application of a recently developed dual genetic lineage-tracing approach for cell fate studies. We place an emphasis on discussing the technical strengths and caveats of different methods, with the goal to develop more specific and efficient tracing technologies for cell fate mapping. Our review also provides several examples for how to use different types of DNA recombinase–mediated lineage-tracing strategies to improve the resolution of the cell fate mapping in order to probe and explore cell fate–related biological phenomena in the life sciences.

Imaging specialist produces clearest pictures yet from dramatic Nasa mission

Former Manchester United and England stalwart Rio Ferdinand says any Government decision regarding the fate of the 2019-20 domestic football season must be respected.

Fresh demonstrations have broken out in Iran, where protesters who took to the streets last weekend in the country's northwestern provinces to demand protection for a dying lake were harshly

Amid lockdown due to the outbreak of coronavirus in the country, Bollywood celebrities are seen doing their own chores at home. Dancer-actress Nora Fatehi seems to be now done as she says she wants the "dishes to leave me alone" in a hilarious post. 

Nora, who is lauded for her flawless dancing skills, took to her Instagram Stories, where she shared a video of herself talking to the pile of dishes kept in the sink. In the TikTok video, Nora seems to have a funny conversation with the dishes in the sink, she is heard saying: "What? You are always looking at me dude! Shut the .... up because I always catch you staring... I swear, ask anybody you are always staring at me."

She captioned the clip: The dishes are always waiting for me... These dishes need to leave me alone #quarantinelife."

 

 

 

View this post on Instagram

@norafatehi this is hilarious! The Dishes! 😂😂😂 Check out Nora’s TikTok for more videos - ItsNoriana 👍🏼❤️ . . #WeLoveNoraFatehi @norafatehifan.england #norafatehi #norafatehifans #garmi #nachinachi #BhujThePrideofIndia #noriana #norafatehifans #pepeta #EkTohKumZindagani #dilbar #arabicdilbar #kamariya #Naah #OSakiSaki #conochallenge #StreetDancer3D #beautiful #love #gorgeous #bae #stunning #dance #fashion #india #mumbai #morocco #actress #bollywood #norafatehifan_england

A post shared by Nora Fatehi Fan | Nora Follows (@norafatehifan.england) onApr 25, 2020 at 9:47am PDT

Recently, a video of Nora's enviable moves went viral, in which she pulled off a fusion of Afro, Urban and Dancehall styles of dancing.

Nora's most popular on-screen performances include "Manohari" ("Baahubali: The Beginning"), "Dilbar" ("Satyamev Jayate"), "O saki saki" ("Batla House") and "Ek toh kam zindagani" ("Marjaavaan"), besides her recent hit "Garmi" in "Street Dancer 3D". On the acting front, Nora will next be seen in the Ajay Devgn-starrer "Bhuj: The Pride Of India".

Catch up on all the latest entertainment news and gossip here. Also, download the new mid-day Android and iOS apps.

Mid-Day is now on Telegram. Click here to join our channel (@middayinfomedialtd) and stay updated with the latest news

(From left) Muazzam and Rizwan

While most Indian music aficionados consider Rahat Fateh Ali Khan as legendary Pakistani Sufi singer Nusrat Fateh Ali Khan's only successor, his nephews Rizwan and Muazzam are also making a mark across the world. The Rizwan-Muazzam Qawwali Group, which comprises the brothers (lead singers), five secondary vocalists, two harmonium players and a tabla player, performed at the recently concluded Gold Coast 2018 Commonwealth Games.

To take their family's five-century-old legacy forward and shoulder the responsibility of being Nusrat Fateh Ali Khan's nephews is no mean feat for the musician duo. "Nusrat saab's music was great. The expectations motivate us to work hard and keep up with his legacy," says Muazzam, as he talks about their greatest learning from their uncle-guru, Khan. "The fact that we can perform in front of a huge audience and impress them with our recitals is because of him. His teachings have helped us master the art of ghazals and qawwalis. Besides being an amazing artist, he was also an amazing teacher and person. Whatever we are today is because of his teachings."

Nusrat Fateh Ali Khan

Though they perform different genres, their specialisation lies in qawwali and ghazals. "We also perform spiritual and contemporary music, and collaborate with musicians from across the world, but qawwalis and ghazals are our first love," says Rizwan.

While Rahat has explored Hindi film music and enjoys a huge fan base in India, the duo is also keen to do more work here. "We enjoyed visiting India a couple of years ago for a multi-city tour. We performed in seven cities, including Mumbai. It was an enriching experience as the audience in every city was warm," says Rizwan, adding that the duo counts Ustad Zakir Hussain, Ranjit Barot, Sivamani and L Subramaniyam among their favourite Indian musicians.

Expressing their wish to venture into Hindi film music, Muazzam says that they would love to collaborate with Indian composers. "Since Bollywood welcomes budding artistes warmly and gives them opportunities, we want to explore Hindi film music soon. In fact, Pritam da reached out to us and expressed an interest in getting us on board for his films. We are looking forward to working with him," he says.

Meanwhile, they are currently exploring the indie music space in India and working on an independent track with an Indian artist — they won't tell us who. "We are also in talks with a few music labels in India," says Muazzam.

Also Read: Rahat Fateh Ali Khan Backs Out Of Recording Fearing Pakistan Backlash

Catch up on all the latest Mumbai news, crime news, current affairs, and also a complete guide on Mumbai from food to things to do and events across the city here. Also download the new mid-day Android and iOS apps to get latest updates

Isle of Man-based Dbay Advisors to charge 18% interest on emergency finance if approved

But its coal addiction and authoritarian system mean it will struggle to take a global lead

The bad blood between mother and daughter culminates in the series finale as Aleida tackles Daya to the ground with her hands around her daughter's neck, leaving her fate uncertain.

Filming of the eighth season of The Bachelor Australia starring Locklan 'Locky' Gilbert was suspended in late March.

Scarlett Johansson has been a fixture of the Marvel Cinematic Universe since 2010, when she first portrayed Natasha Romanoff aka The Black Widow in Iron Man 2.

The former lovebirds took their disagreement to Instagram on Saturday, after Charlotte, 29, shared a cryptic post, accusing her ex of 'taking money' from her.

More than $5million will be refunded to thousands of fans who bought tickets to a basketball that used false advertising to claim LeBron James would be playing.