Many transferable quinolone-resistance mechanisms have been already identified in Gram-negative bacteria. The plasmid-encoded 65 amino-acid long ciprofloxacin-modifying enzyme, namely CrpP, was recently identified in Pseudomonas aeruginosa. We analyzed a collection of 100 clonally-unrelated and multidrug-resistant P. aeruginosa clinical isolates among which 46 (46%) were found positive for crpP-like genes, encoding five CrpP variants conferring variable levels of reduced susceptibility to fluoroquinolones. Those crpP-like genes were chromosomally located, as part of PAGI-like pathogenicity genomic islands.

Background

Optimal concentrations of unbound antimicrobials are essential for maximum microbiological effect. Although hypoalbuminemia and albumin fluid resuscitation are common in critical care, the effects of different albumin concentrations on the unbound concentrations of highly protein-bound antimicrobials are not known. The aim of this study was to compare effects of different albumin states on total and unbound concentrations of ertapenem and ceftriaxone using an ovine model.

Methods

Design

Prospective, three phase intervention observational study.

Subjects

Healthy Merino sheep.

Interventions

Eight sheep were subject to three experimental phases; normoalbuminemia, hypoalbuminemia using plasmapheresis and albumin replacement using a 25% albumin solution. In each phase, ceftriaxone 40 mg/kg and ertapenem 15 mg/kg were given intravenously. Blood samples were collected at pre-defined intervals and analyzed using an ultra-high-performance liquid chromatography tandem mass spectrometry method. Pharmacokinetic parameters such as area under the curve (AUC_0-24), plasma clearance (CL) and apparent volume of distribution in the terminal phase (V_d) were estimated and compared between the phases.

Results

The protein and albumin concentrations were significantly different between phases. Hypoalbuminemia resulted in a significantly lower AUC_0-24 and higher CL of total and unbound concentrations of ceftriaxone compared to the other phases. Whereas albumin replacement led to higher AUC_0-24 and lower CL compared to other phases for both drugs. The V_d for total drug concentrations for both drugs were significantly lower with albumin replacement.

Conclusions

For highly protein-bound drugs such as ceftriaxone and ertapenem, both hypoalbuminemia and albumin replacement may affect unbound drug exposure.

Google has made it easier to join the company's Advanced Protection Program, which is designed to stop the most sophisticated hackers from breaking into your Gmail account. Before you needed two security keys to enroll. Now you just need a smartphone.

The stunning allegation reportedly comes from a forensic analysis Amazon CEO Jeff Bezos commissioned to determine the culprit behind the hack, which resulted in his private photos ending up in the hands of the National Enquirer.

According to a recent forensic analysis, a WhatsApp account from the Saudi Crown Prince Mohammed bin Salman allegedly sent government-bought spyware to Bezos' phone in May 2018, two UN human rights experts said on Wednesday.

OBJECTIVES:

Our first aim was to examine baseline differences in body dissatisfaction, depression, and anxiety symptoms by gender, age, and Tanner (ie, pubertal) stage. Our second aim was to test for changes in youth symptoms over the first year of receiving gender-affirming hormone therapy. Our third aim was to examine potential differences in change over time by demographic and treatment characteristics. Youth experiences of suicidal ideation, suicide attempt, and nonsuicidal self-injury (NSSI) are also reported.

UEFA.com runs the rule over some of the most expensive players in the game to assess who's worth splashing out for.

This list looks at seven top South African products disrupting the traditional way of doing finance.

Student Sandisiwe Msomi saved R23 400 in small change over a year, which she will now use to pay for the registration fees of her second academic year.

Ana L. Creo
Dec 1, 2017; 140:e20171486-e20171486
STATE-OF-THE-ART REVIEW ARTICLES

Remittances from overseas are a lifeline for many South Africans, says Richard Ambrose, CEO of Azimo.

Money Smart Week SA, a financial literacy campaign aimed at motivating and empowering South Africans to become more educated about their finances, is taking place from March 23 to 28, 2020.

Penn State Greater Allegheny student Katarina Shields wanted to help her fellow students seek creative ways to stay connected, make new friends and reduce stress during the remote learning period. The junior IST major developed “Virtual Game Night," a place to game for students attending all Penn State campuses, through the local gamer app Discord. The first event will be held April 18.

With iOS 13.1 and iPadOS, Apple adds an Audio Sharing feature that lets you share with a friend what you're listening to on your Apple AirPods or select Beats headphones.

There are plenty of ways to transfer large files between Windows, Mac, iOS, and Android devices, including AirDrop, Google Photos, Bluetooth transfer, and third-party programs.

Is your favorite music missing on your iPhone or iPad? Maybe you inadvertently turned off syncing or accidentally deleted those tunes. All is not lost; here's how to recover your music.

What you want: space savings, power, and flexibility, all from a single desktop computer. What you need: an all-in-one (AIO) PC. Our buying guide and deep-dive reviews will help you shop for one of these sleek machines. (We've tested all the latest models.)

Incredibly powerful for content creation and gaming, Corsair's One Pro i200 delivers 14-core Intel Core i9 muscle in a stylish design that's a fraction the size of a traditional workstation tower.

Have an important phone call coming up and want to save it to reference later? You can record both incoming and outgoing calls on your Android phone with the right app.

If you want to keep your kids safe online, you need a parental control solution that monitors all of their devices, including phones and tablets. These apps are the top cross-platform performers in our testing.

Alongside a new protected area of storage, OneDrive and Office 365 storage options are changing for the better.

Delaware is among three states using federal grants to develop school and district leaders who understand the complexities of special education.

Reports from the parents of nearly 2,500 children and youths with attention deficit hyperactivity disorder found a gap between students with the most severe symptoms and those who get any school-based interventions.

T. Michael O'Shea
Jul 1, 1999; 104:15-21
ARTICLES

The universe is full of questions waiting to be solved. So why teach science like all the discoveries have already been made? asks Alexander Bell.

Reliable tipster Evan Blass shares details of HMD Global's next budget Android 10 smartphone, which is expected to be called the Nokia 5.2.

On Monday, Pablo Escobar's brother started to take orders for a second foldable phone at the low price of $399. However, users who bought the first foldable phone from the company say their orders have yet to arrive. Instead, what they got was a book and a pamphlet.

The tiny, rugged Atom smartphone is being followed up with the Atom XL, which increases the display size to 4-inches and adds a 48MP camera.

The new foldable phone from Pablo Escobar's brother is a Samsung Galaxy Fold with no changes to the software, according to a YouTuber and a journalist who obtained the products amid consumer worries the business is a scam.

Google's reverse image search is a breeze on a desktop, but what about when you're on a mobile device? Google, Bing, and others have options.

We test and rate hundreds of mobile phones each year. Whether you're looking for Apple, Android, or even a simple feature phone, these are our top picks for a variety of budgets across the major US wireless carriers.

The OnePlus 7T Pro 5G McLaren is a wickedly powerful Android phone which falls just short of the Galaxy S20 series on performance, for considerably less coin.

Chef and food writer Letitia Clark talks Ella Walker through her debut cookbook, Bitter Honey - an ode to the cooking of Sardinia.

Entries from two Penn State students were among winners in categories for professional television journalists as part of the Keystone Media Awards.

A Florida city made the difficult decision to fork over the cash after ransomware hijacked city computers. Everyone needs to make their own choice, but I firmly believe you should never pay the ransom.

When we talk about VPNs, it's often in the context of unsafe situations. Senior Security Analyst Max Eddy argues that these tools are still valuable, even when the risk is low.

Little Richard, the self-proclaimed “architect of rock ‘n’ roll,” has died

Education Week asked three 8th grade teachers to evaluate real student responses to an open-ended question on the National Assessment of Educational Progress in social studies. Here's what they said.

Not exactly an impulse buy but priced well below Wacom pen displays like the Cintiq 16, the Wacom One is a fine product for students, doodlers, and casual artists who'd like to write and sketch digitally.

If you need to scan, copy, or fax in addition to printing, an AIO printer is the way to go. See our top tips on how to find the right multifunction printer, plus our picks for the best all-in-one printers for home and office use.

The cell phone can be a powerful distraction or an educational tool. You have to decide which it will be in your classroom.

053 - UEFA Club Financial Control Body investigatory chamber takes measures

2019 is nearly over, so let's look ahead to what awaits the video game industry in the first year of the new decade. Informed opinions and hot takes abound.

A 20-member church and a volunteer pastor seek long-term help for sharing the gospel with the 24,000 people in their town.

In the 1980s, OM organised large summer campaigns with the huge task of covering every town and village in France with Christian literature.

Playing in the 2002 UEFA European Under-17 Championship finals left a lasting impression on England's record scorer Wayne Rooney - the tournament ambassador for this year's finals in England.

After months of hard work and preparation, nearly a dozen Penn State Hazleton seniors are now one step closer to graduating after presenting their Capstone Research and Design Thesis projects.

As a U.S. senator and vice president, Biden focused on preschool, gun-free school zones, and the Obama administration's response to the Newtown, Conn. school shooting in 2012.

Here's a breakdown of Amazon's revenue from across its e-commerce and retail ecosystem, cloud infrastructure, streaming services, consumer devices, advertising business, and beyond.