Picture 1 show a Shape created in Allegro PCB Editor 16.6, file LTshape.ssm      The Shape Origin is dead center of the outline 565X400 mils.    This Shape is utilized in the PadStack Editor to create a special Pin for a custom Footprint.
Picture 2 show the PadStack Editor first page with drill size and offset. File  LTshape16.pad         Picture 3 show the PadStack Editor page 2 with that LTshape utilized as the BEGIN LAYER for copper area. Picture 4 show the actual Package (footprint) finished with Outline, padstacks for pin 1,2,4,5 and that special Pin 3 LTshape. Its origin is also dead center, same as LTshape.ssm

Notice the round dot at the bottom of Pin 3. It only appear in the Package drawing, not in the original Picture 1, the actual LTshape.ssm or LTshape.dra

Picture 5 shows that same Footprint in the final board in PCB Editor. That same dot, now pink, is still there and cannot be selected separately. When using the Find Option and selecting each object separately, the only way to select Pin 3 is when Option > Pin is selected. Then I can hover on the dot near Pin 3 and the whole LTshape lights up as pin 3, but not the pink dot. There is absolutely no way I can select that dot as an object. There is no way to know the existence of that dot except by looking at it. Cannot be deleted, cannot be selected, can only be invisible if I use the Color Visibility manager and disable all 4 layers, TOP GND VCC BOTTOM. If I turn On TOP then the dot becomes pink. With GND On the dot is green, VCC On will get a Red dot, BOTTOM On will not show the dot but Pin 3 turn On since LTshape is the actual Net connected. This Net is not GND nor VCC, it is N357726.

Where is this dot coming from and why is it not a selectable object ? Why can I not delete it ?

ભારત સરકારે ત્રીજી મે બાદ લૉકડાઉન 3.0ની જાહેરાત કરી છે, જે 17મી મે સુધી ચાલશે. આ દરમિયાન દેશના અલગ અલગ જિલ્લાઓને ઝોન પ્રમાણે વહેંચી દેવામાં આવ્યા છે. આ ઉપરાંત ઝોન પ્રમાણે લૉકડાઉનના નિયમોમાં છૂટછાટ આપવામાં આવી છે. કયા ઝોનમાં શું છૂટછાટ આપવામાં આવી છે તે ઇન્ફોગ્રાફિસથી સમજીઓ...

Seattle, WA-based LevelTen Energy helps corporate buyers of renewable energy find and purchase energy from solar and wind projects within North America through its procurement platform. The company says its solution reduces the cost, complexity, and risk of renewable energy power purchase agreements (PPAs), by incorporating analytics, aggregation, and process best practices.

Today, Smithfield Foods and Roeslein Alternative Energy (RAE) announced that they have formed a joint venture called Monarch Bioenergy to produce renewable natural gas (RNG) across Smithfield’s hog farms in Missouri.

Seattle, WA-based LevelTen Energy helps corporate buyers of renewable energy find and purchase energy from solar and wind projects within North America through its procurement platform. The company says its solution reduces the cost, complexity, and risk of renewable energy power purchase agreements (PPAs), by incorporating analytics, aggregation, and process best practices.

Seattle, WA-based LevelTen Energy helps corporate buyers of renewable energy find and purchase energy from solar and wind projects within North America through its procurement platform. The company says its solution reduces the cost, complexity, and risk of renewable energy power purchase agreements (PPAs), by incorporating analytics, aggregation, and process best practices.

As many of us know all too well, the coronavirus pandemic has forced companies and their personnel to shift their operations to a remote, work-from-home (or WFH) environment. This change has had a profound effect on outsourcing providers and their c...

We acted as legal advisor when the shareholders of Oy Basso Media Ltd. sold all shares of Oy Basso Media Ltd. to Bauer Media Holding Oy. Other financial arrangements were also carried out in connection with the transaction. Basso Media will continue...

Overview On 28 November 2019, the European Banking Authority (“EBA”) published its final guidelines[1] on information and communication technology (“ICT”) and security risk management (EBA/GL/2019/04) (the “Guidelines&r...

The Swiss Federal Supreme Court recently considered whether a dividend distributed by a Swiss company that participated in a zero balancing financing arrangement had been distributed lawfully. The case refers back to 2001 when Swisscargo AG distribu...

In two recent judgments, both rendered on 16 October 2015 (which can be found here and here) the Supreme Court ruled on the sustainability of a legal construct often used by banks called the equity surplus-arrangement. A variant of the...

The IRGC aerospace command takes credit for the achievement and says that it builds on legacies dating back to 1988.

PESHAWAR: Speaker Provincial Assembly Mushtaq Ahmed Ghani visited Khyber Teaching Hospital to take of situation with regard to coronavirus on Friday.He was received by Medical Director Prof Dr Aamir Azhar and the hospital’s media manager. The speaker was briefed by medical director on the...

PESHAWAR: Speaker Provincial Assembly Mushtaq Ahmed Ghani visited Khyber Teaching Hospital to take of situation with regard to coronavirus on Friday.He was received by Medical Director Prof Dr Aamir...

[[ This is a content summary only. Visit my website for full links, other content, and more! ]]

Islamabad : President Arif Alvi on Friday directed that social distancing should be maintained in the upcoming session of the National Assembly for the safety of the parliamentarians and staff against the threat of coronavirus.During a visit to the National Assembly, the president was apprised by...

Enabling Cloud Connectivity to All MCUs and MPUs, Microchip Launches a Range of Embedded IoT Solutions for Rapid Prototyping

The European experience could be useful to inspire the future evolution of the Chiang Mai Initiative Multilateralization in the ASEAN+3 membership.

The European experience could be useful to inspire the future evolution of the Chiang Mai Initiative Multilateralization in the ASEAN+3 membership.

CES 2020 – LAS VEGAS, NV – JANUARY 6, 2020 – When it comes to competitive gaming, sound is survival – and JBL knows great sound. Today at CES, the company unveiled the first-ever JBL headset range and PC speaker designed exclusively for gamers, featuring...

Weird liquid knots can self-assemble into crystals that are tough to untie, which could make for screens that use less energy to store and display information

The defining feature of a crystal is that it is made from regular, repeating blocks, but a chance discovery in an old German book has turned that view on its head

A NASA science balloon picked up two high-energy particles and a new analysis reveals that they can't be explained by the standard model of particle physics

What our senses allow us to experience may not reflect what actually exists. It may be a creation of our own consciousness, or a computer simulation designed by superintelligent beings

Piper Sandler senior technical analyst Craig Johnson says the S&P 500 may trade in a narrow range through the summer before breaking north.

Since the discovery of Asgard archaea in 2015, evidence has mounted that these peculiar single-celled organisms could be the source of all complex life – including us

For orangutans, scratching is contagious – but unexpectedly, the behaviour is transmitted more between individuals that do not know each other well

Kate Darling researches human-robot interaction. She explains why we are prone to forming emotional connections with robots and what we can learn from our relationships with pets and other animals

What our senses allow us to experience may not reflect what actually exists. It may be a creation of our own consciousness, or a computer simulation designed by superintelligent beings

Lose your bearings in an unfamiliar landscape and fear shreds your navigational brain. But studies are now revealing the common mistakes lost people make, helping rescue teams to find them before it’s too late

Our images of deep space are spectacular, but don’t reflect what our eyes would see. Here's what their stunning true colours reveal about the cosmos

For orangutans, scratching is contagious – but unexpectedly, the behaviour is transmitted more between individuals that do not know each other well

Piper Sandler senior technical analyst Craig Johnson says the S&P 500 may trade in a narrow range through the summer before breaking north.

ABSTRACT

Lassa virus (LASV) poses a significant public health problem within the regions of Lassa fever endemicity in Western Africa. LASV infects several hundred thousand individuals yearly, and a considerable number of Lassa fever cases are associated with high morbidity and lethality. No approved LASV vaccine is available, and current therapy is limited to an off-label usage of ribavirin that is only partially effective and associated with significant side effects. The impact of Lassa fever on human health, together with the limited existing countermeasures, highlights the importance of developing effective vaccines against LASV. Here, we present the development and characterization of a recombinant LASV (rLASV) vaccine candidate [rLASV(IGR/S-S)], which is based on the presence of the noncoding intergenic region (IGR) of the small (S) genome segment (S-IGR) in both large (L) and S LASV segments. In cultured cells, rLASV(IGR/S-S) was modestly less fit than wild-type rLASV (rLASV-WT). rLASV(IGR/S-S) was highly attenuated in guinea pigs, and a single subcutaneous low dose of the virus completely protected against otherwise lethal infection with LASV-WT. Moreover, rLASV(IGR/S-S) was genetically stable during serial passages in cultured cells. These findings indicate that rLASV(IGR/S-S) can be developed into a LASV live-attenuated vaccine (LAV) that has the same antigenic composition as LASV-WT and a well-defined mechanism of attenuation that overcomes concerns about increased virulence that could be caused by genetic changes in the LAV during multiple rounds of multiplication.

IMPORTANCE Lassa virus (LASV), the causative agent of Lassa fever, infects several hundred thousand people in Western Africa, resulting in many lethal Lassa fever cases. No U.S. Food and Drug Administration-licensed countermeasures are available to prevent or treat LASV infection. We describe the generation of a novel LASV live-attenuated vaccine candidate rLASV(IGR/S-S), which is based on the replacement of the large genomic segment noncoding intergenic region (IGR) with that of the small genome segment. rLASV(IGR/S-S) is less fit in cell culture than wild-type virus and does not cause clinical signs in inoculated guinea pigs. Importantly, rLASV(IGR/S-S) protects immunized guinea pigs against an otherwise lethal exposure to LASV.

ABSTRACT

Legionella pneumophila governs its interactions with host cells by secreting >300 different "effector" proteins. Some of these effectors contain eukaryotic domains such as the RCC1 (regulator of chromosome condensation 1) repeats promoting the activation of the small GTPase Ran. In this report, we reveal a conserved pattern of L. pneumophila RCC1 repeat genes, which are distributed in two main clusters of strains. Accordingly, strain Philadelphia-1 contains two RCC1 genes implicated in bacterial virulence, legG1 (Legionella eukaryotic gene 1), and ppgA, while strain Paris contains only one, pieG. The RCC1 repeat effectors localize to different cellular compartments and bind distinct components of the Ran GTPase cycle, including Ran modulators and the small GTPase itself, and yet they all promote the activation of Ran. The pieG gene spans the corresponding open reading frames of legG1 and a separate adjacent upstream gene, lpg1975. legG1 and lpg1975 are fused upon addition of a single nucleotide to encode a protein that adopts the binding specificity of PieG. Thus, a point mutation in pieG splits the gene, altering the effector target. These results indicate that divergent evolution of RCC1 repeat effectors defines the Ran GTPase cycle targets and that modulation of different components of the cycle might fine-tune Ran activation during Legionella infection.

IMPORTANCE Legionella pneumophila is a ubiquitous environmental bacterium which, upon inhalation, causes a life-threatening pneumonia termed Legionnaires’ disease. The opportunistic pathogen grows in amoebae and macrophages by employing a "type IV" secretion system, which secretes more than 300 different "effector" proteins into the host cell, where they subvert pivotal processes. The function of many of these effector proteins is unknown, and their evolution has not been studied. L. pneumophila RCC1 repeat effectors target the small GTPase Ran, a molecular switch implicated in different cellular processes such as nucleocytoplasmic transport and microtubule cytoskeleton dynamics. We provide evidence that one or more RCC1 repeat genes are distributed in two main clusters of L. pneumophila strains and have divergently evolved to target different components of the Ran GTPase activation cycle at different subcellular sites. Thus, L. pneumophila employs a sophisticated strategy to subvert host cell Ran GTPase during infection.

BEAF (Boundary Element-Associated Factor) was originally identified as a Drosophila melanogaster chromatin domain insulator-binding protein, suggesting a role in gene regulation through chromatin organization and dynamics. Genome-wide mapping found that BEAF usually binds near transcription start sites, often of housekeeping genes, suggesting a role in promoter function. This would be a nontraditional role for an insulator-binding protein. To gain insight into molecular mechanisms of BEAF function, we identified interacting proteins using yeast two-hybrid assays. Here, we focus on the transcription factor Serendipity (Sry-). Interactions were confirmed in pull-down experiments using bacterially expressed proteins, by bimolecular fluorescence complementation, and in a genetic assay in transgenic flies. Sry- interacted with promoter-proximal BEAF both when bound to DNA adjacent to BEAF or > 2-kb upstream to activate a reporter gene in transient transfection experiments. The interaction between BEAF and Sry- was detected using both a minimal developmental promoter (y) and a housekeeping promoter (RpS12), while BEAF alone strongly activated the housekeeping promoter. These two functions for BEAF implicate it in playing a direct role in gene regulation at hundreds of BEAF-associated promoters.

Triple-negative breast cancer (TNBC) accounts for 10 to 20% of breast cancer, with chemotherapy as its mainstay of treatment due to lack of well-defined targets, and recent genomic sequencing studies have revealed a paucity of TNBC-specific mutations. Recurrent gene fusions comprise a class of viable genetic targets in solid tumors;...

The most fundamental genetic program of an annual plant defines when to grow and reproduce and when to remain dormant in the soil as a seed. With the right timing, plants can even live in hostile regions with only a few months of growth-favorable abundant rains and mild temperatures. To...

Mixed lineage leukemia (MLL/KMT2A) rearrangements (MLL-r) are one of the most frequent chromosomal aberrations in acute myeloid leukemia. We evaluated the function of Meningioma 1 (MN1), a co-factor of HOXA9 and MEIS1, in human and murine MLL-rearranged leukemia by CRISPR-Cas9 mediated deletion of MN1. MN1 was required for in vivo leukemogenicity of MLL positive murine and human leukemia cells. Loss of MN1 inhibited cell cycle and proliferation, promoted apoptosis and induced differentiation of MLL-rearranged cells. Expression analysis and chromatin immunoprecipitation with sequencing from previously reported data sets demonstrated that MN1 primarily maintains active transcription of HOXA9 and HOXA10, which are critical downstream genes of MLL, and their target genes like BCL2, MCL1 and Survivin. Treatment of MLL-rearranged primary leukemia cells with anti-MN1 siRNA significantly reduced their clonogenic potential in contrast to normal CD34⁺ hematopoietic progenitor cells, suggesting a therapeutic window for MN1 targeting. In summary, our findings demonstrate that MN1 plays an essential role in MLL fusion leukemias and serve as a therapeutic target in MLL-rearranged acute myeloid leukemia.

BACKGROUND:

Prescribing of levothyroxine and rates of thyroid function testing may be sensitive to minor changes in the upper limit of the reference range for thyroid-stimulating hormone (TSH) that increase the proportion of abnormal results. We evaluated the population-level change in levothyroxine prescribing and TSH testing after a minor planned decrease in the upper limit of the reference range for TSH in a large urban centre with a single medical laboratory.

Kate Darling researches human-robot interaction. She explains why we are prone to forming emotional connections with robots and what we can learn from our relationships with pets and other animals

NASA has selected four potential future missions – to Jupiter’s fiery moon Io, Neptune’s icy moon Triton, and two that would explore the atmosphere and map the surface of Venus

Our images of deep space are spectacular, but don’t reflect what our eyes would see. Here's what their stunning true colours reveal about the cosmos

Venus’s surface is covered in strange wiggling lines that may form when colossal stacks of lava are carved by gentle winds over hundreds of millions of years

Stranger Things has grown to become one of the most...

The post Stranger Things Season 4 Will Be “A Lot Scarier,” Says Joe Keery appeared first on Man's World India.

UK hospital trusts have been advised to "risk-assess" black, Asian and minority ethnic (BAME) members of staff and make "appropriate arrangements" as data emerged suggesting they are disproportionately affected by Covid-19.

A north London teenager, 16, has been found guilty of kidnapping a stranger in the street and forcing him to withdraw money from a cash machine.

She's perhaps best known to the internet as the woman who wore a face of mild disbelief when US President Donald Trump discussed disinfectant as a possible treatment for coronavirus.