It’s never too late to start getting connected online

MediaTek Dimensity 1080 has 200MP camera with Imagiq ISP

Satirical films about the media have always been entertaining

Punjab Kings co-owner Ness Wadia congratulated Jay Shah and Co for delivering on an innovative media rights strategy but hoped the IPL would have more games over two halves in a calendar year

Why has digital streaming rights garnered higher numbers in the auction? How has the league’s value improved?

'Rohit and amp; Agarkar, much better guys to front up for the media.'

Company: Vcare Call Centers India Pvt Ltd
Experience: 1 to 6
Salary: 2.30 to 4.00
location: Noida
Ref: 24828927
Summary: Need Social Media Executive to Interact with customers on Social Media Platforms (Should have Excellent Writing Skills) Laptop & High Speed Internet (Wifi) Required - 5 Days working -Noida Location....

Company: SP Staffing Services Private Limited
Qualification: Other
Experience: 1 to 5
Salary: 3.50 to 6.10
location: Chennai
Ref: 24374534
Summary: Immediate Hiring For Python Developer

A trailblazing satirist who battled racial injustice in America

HDFC Bank says “minuscule” number of employees told to leave as part of annual appraisal process

South Dakota Governor Kristi Noem sent letters to two Sioux tribes demanding they remove COVID-19 checkpoints because they interfere with traffic.

In a tweet Saturday morning, Tesla's chief executive said it would file a lawsuit against county officials over not being able to run its factory.

Although a robust inflammatory response is needed to combat infection, this response must ultimately be terminated to prevent chronic inflammation. One mechanism that terminates inflammatory signaling is the production of alternative mRNA splice forms in the Toll-like receptor (TLR) signaling pathway. Whereas most genes in the TLR pathway encode positive mediators of inflammatory signaling, several, including that encoding the MyD88 signaling adaptor, also produce alternative spliced mRNA isoforms that encode dominant-negative inhibitors of the response. Production of these negatively acting alternatively spliced isoforms is induced by stimulation with the TLR4 agonist lipopolysaccharide (LPS); thus, this alternative pre-mRNA splicing represents a negative feedback loop that terminates TLR signaling and prevents chronic inflammation. In the current study, we investigated the mechanisms regulating the LPS-induced alternative pre-mRNA splicing of the MyD88 transcript in murine macrophages. We found that 1) the induction of the alternatively spliced MyD88 form is due to alternative pre-mRNA splicing and not caused by another RNA regulatory mechanism, 2) MyD88 splicing is regulated by both the MyD88- and TRIF-dependent arms of the TLR signaling pathway, 3) MyD88 splicing is regulated by the NF-κB transcription factor, and 4) NF-κB likely regulates MyD88 alternative pre-mRNA splicing per se rather than regulating splicing indirectly by altering MyD88 transcription. We conclude that alternative splicing of MyD88 may provide a sensitive mechanism that ensures robust termination of inflammation for tissue repair and restoration of normal tissue homeostasis once an infection is controlled.

Inter-α-inhibitor is a proteoglycan essential for mammalian reproduction and also plays a less well-characterized role in inflammation. It comprises two homologous “heavy chains” (HC1 and HC2) covalently attached to chondroitin sulfate on the bikunin core protein. Before ovulation, HCs are transferred onto the polysaccharide hyaluronan (HA) to form covalent HC·HA complexes, thereby stabilizing an extracellular matrix around the oocyte required for fertilization. Additionally, such complexes form during inflammatory processes and mediate leukocyte adhesion in the synovial fluids of arthritis patients and protect against sepsis. Here using X-ray crystallography, we show that human HC1 has a structure similar to integrin β-chains, with a von Willebrand factor A domain containing a functional metal ion-dependent adhesion site (MIDAS) and an associated hybrid domain. A comparison of the WT protein and a variant with an impaired MIDAS (but otherwise structurally identical) by small-angle X-ray scattering and analytical ultracentrifugation revealed that HC1 self-associates in a cation-dependent manner, providing a mechanism for HC·HA cross-linking and matrix stabilization. Surprisingly, unlike integrins, HC1 interacted with RGD-containing ligands, such as fibronectin, vitronectin, and the latency-associated peptides of transforming growth factor β, in a MIDAS/cation-independent manner. However, HC1 utilizes its MIDAS motif to bind to and inhibit the cleavage of complement C3, and small-angle X-ray scattering–based modeling indicates that this occurs through the inhibition of the alternative pathway C3 convertase. These findings provide detailed structural and functional insights into HC1 as a regulator of innate immunity and further elucidate the role of HC·HA complexes in inflammation and ovulation.

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

Contact between inflammatory cells and endothelial cells (ECs) is a crucial step in vascular inflammation. Recently, we demonstrated that the cell-surface level of endomucin (EMCN), a heavily O-glycosylated single-transmembrane sialomucin, interferes with the interactions between inflammatory cells and ECs. We have also shown that, in response to an inflammatory stimulus, EMCN is cleared from the cell surface by an unknown mechanism. In this study, using adenovirus-mediated overexpression of a tagged EMCN in human umbilical vein ECs, we found that treatment with tumor necrosis factor α (TNF-α) or the strong oxidant pervanadate leads to loss of cell-surface EMCN and increases the levels of the C-terminal fragment of EMCN 3- to 4-fold. Furthermore, treatment with the broad-spectrum matrix metalloproteinase inhibitor batimastat (BB94) or inhibition of ADAM metallopeptidase domain 10 (ADAM10) and ADAM17 with two small-molecule inhibitors, GW280264X and GI254023X, or with siRNA significantly reduced basal and TNFα-induced cell-surface EMCN cleavage. Release of the C-terminal fragment of EMCN by TNF-α treatment was blocked by chemical inhibition of ADAM10 alone or in combination with ADAM17. These results indicate that cell-surface EMCN undergoes constitutive cleavage and that TNF-α treatment dramatically increases this cleavage, which is mediated predominantly by ADAM10 and ADAM17. As endothelial cell-surface EMCN attenuates leukocyte–EC interactions during inflammation, we propose that EMCN is a potential therapeutic target to manage vascular inflammation.

Realme Narzo 10 confirmed to feature MediaTek Helio G80 gaming processor  Hindustan TimesRealme Narzo 10 officially confirmed to pack Helio G80 SoC - GSMArena.com news  GSMArena.comRealme Narzo 10, Realme Narzo 10A Launch on May 11:...

OM Philippines partners with the local church to provide water and food in areas hit by the 7.2 magnitude earthquake on 15 October.

Milena found mission opportunities on OM Germany’s website, which led her to share the love of God in Belgium.

Mindshare and GroupM have implemented their own tools that are better than the industry standards, and we use our own competitive advantage to give our clients better insights than they could get through an industry model.

This Metasploit module exploits a stack buffer overflow in the Windows Media Unicast Service version 4.1.0.3930 (NUMS.exe). By sending a specially crafted FunnelConnect request, an attacker can execute arbitrary code under the "NetShowServices" user account. Windows Media Services 4.1 ships with Windows 2000 Server, but is not installed by default. NOTE: This service does NOT restart automatically. Successful, as well as unsuccessful exploitation attempts will kill the service which prevents additional attempts.

The UN climate conference in Lima set the stage for Paris in 2015. Next year’s accord is to provide a working, albeit not a final, answer to the question: Is it possible to keep global warming at or below the 2 degree Celsius limit? This limit is considered the boundary beyond which the negative climatic, economic and social consequences of climate change are thought to become intolerably severe and potentially irreversible.

The Pentecostal Fellowship of Nigeria, PFN, in Edo State, has called on the state government to reopen churches. PFN explained that reopening churches in the state would help create more awareness on how to curb COVID-19. The body said churches would embark on aggressive intercession for divine intervention against coronavirus if they are reopened. In […]

PFN gives government reasons to immediately reopen churches amid COVID-19 pandemic

Athar Shah Khan began his career at Radio Pakistan as a writer and had 700 plays under his belt

RAWALPINDI: Punjab Information Minister Fayyaz-ul-Hassan Chohan Saturday said that Information Department would give Rs100,000 to media worker affected by COVID-19 while in case of an unfortunate...

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Palo Alto, CA – Aha™ by HARMAN today announced new content available via the free Aha Radio app for Android and iOS and included in the in-vehicle infotainment system in over 40 models of cars. With the addition of self-improvement publisher Hay House, live podcast network BlogTalkRadio and leading national broadcasters American Public Media (APM) and CBC/Radio-Canada, Aha adds thousands of new programs, across a multitude of genres, extending its content library of 40,000 audio and non-audio programs to include exactly what consumers want to hear in the car, at home, and on the go.Specific offerings include APM’s business and economic news program Marketplace, AAA station The Current, Classical Minnesota Public Radio and regional news from Southern California Public Radio and Minnesota Public Radio.

CES 2014, LAS VEGAS -- HARMAN announced today that it is working with Emmis Communications, Salem Communications and MediaTracks Communications, integrating top radio stations and digital content into its Aha Radio platform. The arrangements further expand Aha Radio content offerings to its global user base, delivering an extensive and diverse mix of terrestrial and digital radio content. Drivers outside major U.S. cities can now tune in to these top radio stations and digital content in their car through the Aha app.

(Clarifies in paragraph 18 that some UK newspapers carried images of Charlie Hebdo front pages)

Media companies that are starting to allow their programs onto Internet-delivered TV and mobile devices are putting limits on digital rights as a safety hatch if problems arise with the new distribution systems, executives say.

Media companies that are starting to allow their programs onto Internet-delivered TV and mobile devices are putting limits on digital rights as a safety hatch if problems arise with the new distribution systems, executives say.

Two races into his much-publicised comeback and Michael Schumacher is already feeling the heat after finishing tenth out of 14 finishers at the Australian Grand Prix.

You may not have even been acquainted to the thought of social media management in recent past, however in today’s world you will hear it spoken of from presumably every one. A number of us use this expression as we have a...

Immediate action is needed to address the challenge of excess global steel capacity, according to industry and government officials at the OECD’s Steel Committee meeting in Paris on 30 November and 1 December 2015.