community COMMUNITY TRANSPORTATION FUND, CITY OF WILMINGTON, 2020 OPEN END By www.deldot.gov Published On :: Fri, 03 Apr 2020 04:00:00 GMT COMMUNITY TRANSPORTATION FUND, CITY OF WILMINGTON, 2020 OPEN END Full Article
community COMMUNITY TRANSPORTATION FUND, NORTH IV, 2020 OPEN END By www.deldot.gov Published On :: Thu, 09 Apr 2020 04:00:00 GMT COMMUNITY TRANSPORTATION FUND, NORTH IV, 2020 OPEN END Full Article
community Community Assistance Sought By Uitenhage Detectives By allafrica.com Published On :: Fri, 08 May 2020 15:36:11 GMT [SAPS] Detectives attached to the South African Police Service in Uitenhage are investigating an inquest after the body of a female baby was found amongst the refuse at the Koedoeskloof refuse sight at about 08:00 on Monday, 4 May 2020. A vagrant was busy searching through the rubbish bags, when he made the discovery. A post mortem will be held to determine the exact cause of death. The circumstances surrounding the discovery and how the body landed up amongst the refuse is not clear and it is under investigation Full Article
community QRadar Community Edition 7.3.1.6 Arbitrary Object Instantiation By packetstormsecurity.com Published On :: Tue, 21 Apr 2020 20:15:08 GMT QRadar Community Edition version 7.3.1.6 is vulnerable to instantiation of arbitrary objects based on user-supplied input. An authenticated attacker can abuse this to perform various types of attacks including server-side request forgery and (potentially) arbitrary execution of code. Full Article
community QRadar Community Edition 7.3.1.6 CSRF / Weak Access Control By packetstormsecurity.com Published On :: Tue, 21 Apr 2020 19:57:06 GMT QRadar Community Edition version 7.3.1.6 suffers from cross site request forgery and weak access control vulnerabilities. Full Article
community QRadar Community Edition 7.3.1.6 PHP Object Injection By packetstormsecurity.com Published On :: Tue, 21 Apr 2020 20:13:50 GMT QRadar Community Edition version 7.3.1.6 suffers from a php object injection vulnerability. Full Article
community QRadar Community Edition 7.3.1.6 Authorization Bypass By packetstormsecurity.com Published On :: Tue, 21 Apr 2020 20:28:15 GMT QRadar Community Edition version 7.3.1.6 suffers from an authorization bypass vulnerability. Full Article
community QRadar Community Edition 7.3.1.6 Path Traversal By packetstormsecurity.com Published On :: Tue, 21 Apr 2020 20:30:41 GMT QRadar Community Edition version 7.3.1.6 has a path traversal that exists in the session validation functionality. In particular, the vulnerability is present in the part that handles session tokens (UUIDs). QRadar fails to validate if the user-supplied token is in the correct format. Using path traversal it is possible for authenticated users to impersonate other users, and also to executed arbitrary code (via Java deserialization). The code will be executed with the privileges of the Tomcat system user. Full Article
community QRadar Community Edition 7.3.1.6 Insecure File Permissions By packetstormsecurity.com Published On :: Tue, 21 Apr 2020 20:09:24 GMT QRadar Community Edition version 7.3.1.6 suffers from a local privilege escalation due to insecure file permissions with run-result-reader.sh. Full Article
community 75 MW of community solar coming to Illinois in Ameren, ComEd territories By feedproxy.google.com Published On :: 2019-07-11T14:23:23Z Arlington, VA based Summit Ridge Energy (SRE) announced that it will acquire 11 projects from Pivot Energy in Illinois totaling 29 MWs, increasing SRE’s portfolio of community solar projects in the state to more than 20. Full Article DER Microgrids News Solar Community Solar
community How car insurers could help connect community solar with EV customers By feedproxy.google.com Published On :: 2019-04-29T09:35:00Z The insurance industry has lots of exposure to climate change. But as Warren Buffet has explained, not so much for companies that do annual policy adjustments, like Berkshire Hathaway. Their exposure is limited because the trends are baked into the premiums. But there is an opportunity for reducing insurance risk due to climate change, and it comes from the insurance industry itself. The business model is to have car insurance salespeople provide leads to virtual electric car charging services. This has perfect demographics because electric vehicle owners are very receptive to solar electricity. Who wouldn’t want a clean transportation solution with local job creation that can’t be offshore? Full Article News Vehicle to grid Storage Solar Infrastructure Community Solar
community Clearing up some confusion over community solar in New York By feedproxy.google.com Published On :: 2019-05-06T14:45:08Z Community Solar in New York has a messaging problem. It is confusing, and even some industry professionals have given up in disgust because of aggressive marketing and a lack of clarity. Full Article DER Microgrids News Solar Utility Integration Community Solar
community 20 MW of community solar available for purchase by Xcel customers in Minnesota By feedproxy.google.com Published On :: 2019-05-17T15:34:02Z CleanChoice Energy on Thursday said it was opening an additional 20.51 MW of community solar capacity for residential customers of Xcel. The new capacity is being supplied by fifteen solar farms owned and operated by AltusPower America in various locations in Minnesota. CleanChoice Energy is the largest community solar provider in Minnesota with more than 85 megawatts of solar capacity. Full Article DER Rooftop Solar Utility Integration Community Solar
community 75 MW of community solar coming to Illinois in Ameren, ComEd territories By feedproxy.google.com Published On :: 2019-07-11T14:23:23Z Arlington, VA based Summit Ridge Energy (SRE) announced that it will acquire 11 projects from Pivot Energy in Illinois totaling 29 MWs, increasing SRE’s portfolio of community solar projects in the state to more than 20. Full Article DER Microgrids News Solar Community Solar
community Sustainable Women Series: How to Build a 75 Percent Net Zero Community By feedproxy.google.com Published On :: 2016-06-07T20:29:00Z What does it take to build a 75 percent net zero community and the “largest Emerald-rated community” in the world? Tabitha Crawford explains how her team combined solar, HVAC, and sustainable building practices to build 250 net zero homes while keeping construction costs at a 3 percent premium. Full Article Energy Efficiency Energy Efficiency Rooftop Solar
community Forecasting the Energy Community: Open Call for the Inaugural Season of a Fantasy Energy League By feedproxy.google.com Published On :: 2018-12-07T12:23:42Z Fantasy sports and the energy industry might not have much in common on the surface, but I’ve always personally approached these two passions of mine in similar ways: obsessively reading the breaking news, following my favorite experts in the community on social media, and diving deep into the available statistics to create graphs and try to come up with hot takes. I think the fantasy sports model can be used to encourage an academic and educational exercise in the energy industry, so it struck me—I should establish the first fantasy league for the energy sector! Full Article Hydropower Storage Energy Efficiency Bioenergy Wind Power Opinion & Commentary Solar Geothermal
community How car insurers could help connect community solar with EV customers By feedproxy.google.com Published On :: 2019-04-29T09:35:00Z The insurance industry has lots of exposure to climate change. But as Warren Buffet has explained, not so much for companies that do annual policy adjustments, like Berkshire Hathaway. Their exposure is limited because the trends are baked into the premiums. But there is an opportunity for reducing insurance risk due to climate change, and it comes from the insurance industry itself. The business model is to have car insurance salespeople provide leads to virtual electric car charging services. This has perfect demographics because electric vehicle owners are very receptive to solar electricity. Who wouldn’t want a clean transportation solution with local job creation that can’t be offshore? Full Article News Vehicle to grid Storage Solar Infrastructure Community Solar
community Spotlight on Public Events: Microfinance as a Tool for Community Development By feedproxy.google.com Published On :: Thu, 07 Apr 2011 08:35:28 +0000 Spotlight on Public Events: Microfinance as a Tool for Community Development EWC Changing Faces Women’s Leadership Program participant Kalpana Sankar, CEO of Hand in Hand in Tamil Nadu (India), speaking on microfinance initiatives. Kathleen Stack, Vice President of Freedom from Hunger and a participant in EWC’s 2009 Changing Faces Women’s Leadership Program, speaking on her NGO’s microfinance projects. Full Article
community Spotlight on Publications: East-West Dialogue Discusses the U.S. Role in Fostering the ASEAN Economic Community By feedproxy.google.com Published On :: Thu, 07 Apr 2011 08:35:42 +0000 Spotlight on Publications: East-West Dialogue Discusses the U.S. Role in Fostering the ASEAN Economic Community In the latest issue of the East-West Dialogue, How (and Why) the United States Should Help to Build the ASEAN Economic Community , EWC Senior Fellow Michael Plummer calls for ambitious new initiatives, leading to a U.S.-ASEAN Economic Space that would develop a framework for expanding FTAs and a U.S.-ASEAN Partnership Fund to deepen civic and cultural ties. These ideas are elaborated on in commentaries by Scot Marciel (U.S. Ambassador to ASEAN), Kishore Mahbubani (former Singaporean Ambassador to the UN), and Chalongphob Sussangkarn (former Thai Minister of Finance). Full Article
community EWC Community Saddened by Loss of Prof. Jon Van Dyke By feedproxy.google.com Published On :: Fri, 02 Dec 2011 03:49:55 +0000 The East-West Center community was saddened to learn of the sudden death on Nov. 29 of Adjunct Senior Fellow Jon Van Dyke, a University of Hawaii law professor and longtime associate of the Center. He was a leading authority on constitutional, international and maritime law, as well as indigenous and environmental issues. Recently, he participated in an EWC-organized meeting of supreme court justices from ASEAN countries. Our thoughts and deepest condolences go to Jon’s wife Sherry, his family and his many friends, students and colleagues. The University of Hawaii’s William S. Richardson School of Law has created a tribute page, where those who wish to do so may share their condolences and memories. Full Article
community EWC Community Saddened by Loss of Emeritus Senior Fellow Meheroo Jussawalla By feedproxy.google.com Published On :: Tue, 31 Jul 2012 23:44:07 +0000 The East-West Center expresses its sadness over the passing of Emeritus Senior Fellow Meheroo Jussawalla following a long illness. After a very distinguished career in her native India, Meheroo joined the East-West Center’s Communications Institute in 1977 and was a pioneer in developing the field of information economic. After she formally retired, she remained active in East-West Center projects and activities. Full Article
community EWC Honors Governor Ariyoshi with Asia Pacific Community Building Award By feedproxy.google.com Published On :: Thu, 04 Oct 2012 20:04:44 +0000 Governor and Mrs. Ariyoshi with Rick Tsujimura, EWC Board of Governors' Chairman, at the EWC award banquet.The East-West Center honored the Honorable George R. Ariyoshi, former Governor of the State of Hawai'i, with the presentation of the Asia Pacific Community Building Award for his dedication to strengthening the bonds of understanding among the peoples and nations of Asia, the Pacific, and the United States. The Award recognizes outstanding leaders whose vision and professional and personal accomplishments exemplify the mission of the East-West Center. Full Article
community EWC Community Mourns Passing of Senator Inouye By feedproxy.google.com Published On :: Fri, 21 Dec 2012 21:20:29 +0000 Sen. Inouye with EWC students in 2007. The East-West Center community is greatly saddened by the passing of Hawai‘i Senator Daniel K. Inouye, long one of the Center’s most avid supporters. EWC President Charles E. Morrison extended the EWC community’s heartfelt condolences to Senator Inouye’s family and loved ones. “We are deeply saddened by the loss of a great patriot and statesman who served his nation and state in many different capacities,” Morrison said. “Senator Inouye has always been the staunchest supporter of the East-West Center, not just in Washington, but also frequently interacting with participants and alumni. We will sorely miss him.” Full Article
community EWC Community Saddened by Passing of Center's First Leader, Longtime Arts Coordinator By feedproxy.google.com Published On :: Wed, 03 Sep 2014 02:35:49 +0000 The East-West Center community is saddened by news of the recent passing of the Center’s first leader, Murray Turnbull, and longtime arts and exhibits coordinator Jeanette "Benji" Bennington. "Murray Turnbull was the father of the concept of bringing the young people of the Asia Pacific region together, and the East-West Center was established because of him," said EWC President Charles E. Morrison. "And Benji was an incredible, invaluable resource during her decades of service at the Center. She embodied the EWC spirit, and her legacy remains with us all.” Full Article
community EWC Community Saddened by Sudden Passing of Larry Smith By feedproxy.google.com Published On :: Wed, 17 Dec 2014 01:09:47 +0000 The East-West Center community has been saddened to learn of the sudden passing of former Education Director and longtime community supporter Larry Smith, who suffered a fatal heart attack over the weekend, shortly after arriving in New Delhi to attend a conference. Full Article
community EWC Community Members Aid Voyaging Canoe Hokule‘a’s Unprecedented Visit to Bali By feedproxy.google.com Published On :: Tue, 04 Aug 2015 02:10:49 +0000 HONOLULU (Aug. 3, 2015) -- With the Hawaiian Voyaging Canoe Hokule‘a now sailing out of the Pacific for the very first time and currently in Bali, Indonesia, for the canoe’s only scheduled Asian stop on its unprecedented worldwide voyage, a number of East-West Center participants, alumni and community members are in Bali with the canoe, or helped plan the visit. Full Article
community EWC Community Saddened by Passing of Former Dean of Students Sumi Makey By feedproxy.google.com Published On :: Tue, 22 Oct 2019 22:14:45 +0000 HONOLULU (October 22, 2019) -- The East-West Center community has been saddened to learn of the passing on October 20 of former Dean of Students and longtime Center supporter Sumi Makey. Generations of EWC alumni carry warm memories of Sumi as a caring mentor over the course of her several decades leading the Center’s student and Open Grants programs, which she helped to establish, and later as an active supporter and donor. (Read Sumi’s EWC oral history.) Full Article
community Education e-briefing - Are your assets of Community Value? By www.eversheds.com Published On :: 2018-01-15 The Community Right to Bid has now been in place for half a decade and whilst its impact has perhaps not been as significant as first thought it has undoubtedly had an impact on assets linked to higher education institutions such as playing fields, ... Full Article
community International Development Experts to Discuss Microfinance For Community Development By feedproxy.google.com Published On :: Thu, 07 Apr 2011 08:32:23 +0000 International Development Experts to Discuss Microfinance For Community Development HONOLULU (August 20, 2009) – Many developing nations are now using microfinance as a means to reduce poverty. On Tuesday, September 1, the East-West Center (EWC) will host a public forum on “Microfinance as a Tool for Community Development” in the EWC Gallery featuring presentations by the following international development experts: Full Article
community EWC to Coordinate 5-State Community College Initiative on Asian Studies By feedproxy.google.com Published On :: Fri, 02 Mar 2012 01:07:09 +0000 HONOLULU (March 1, 2012) -- The East-West Center has received a $360,000 award from the National Endowment for the Humanities for a collaborative project to work with 15 community colleges in five states on developing Asian studies curricula. Full Article
community East-West Center Awarded $267,000 Grant for U.S.-Japan Grassroots Exchange on Post-Disaster Community Building By feedproxy.google.com Published On :: Thu, 19 Feb 2015 00:35:31 +0000 Honolulu (Feb. 12, 2015) -- The East-West Center has been awarded a grant of $267,186 from the Japan Foundation’s Center for Global Partnership for a three-year dialogue and travel-exchange program focusing on community participation in long-term recovery after a major disaster. Participants from the cities of Kobe and Kesennuma in Japan, and New Orleans and Galveston in the United States, will share experiences, perspectives, and best practices for increasing citizen input on post-disaster policy and decision-making. Full Article
community East-West Center Community Responds to Nepal Quake By feedproxy.google.com Published On :: Fri, 01 May 2015 02:03:43 +0000 Photo by EWC Changing Faces Women's LeadershipSeminar alumna Luna K. C. Ghimire. Full Article
community Community Infrastructure Levy: navigating the minefield By www.eversheds.com Published On :: 2012-03-07 The introduction of Community Infrastructure Levy (CIL) charging schedules by many local authorities is now gathering pace, as is demonstrated by the Mayor of London’s CIL charging schedule which is likely to be approved by 1 April 2012. If th... Full Article
community Community Infrastructure Levy By www.eversheds.com Published On :: 2008-03-05 The Department of Communities and Local Government has published new guidance explaining how the Community Infrastructure Levy (CIL) will work in practice. The guidance covers: how CIL will be set and spent how, when and by whom CIL will be... Full Article
community Coronavirus takes a toll in Sweden's immigrant community By www.startribune.com Published On :: 2020-05-09T12:32:34+00:00 The flight from Italy was one of the last arrivals that day at the Stockholm airport. A Swedish couple in their 50s walked up and loaded their skis into Razzak Khalaf's taxi. Full Article
community Delta CP wades into Delta community crisis By www.vanguardngr.com Published On :: Sat, 09 May 2020 13:59:36 +0000 …directs Area Commander, DPO to disarm disbanded vigilante members By Festus Ahon ASABA – DELTA State Commissioner of Police, Mr Hafiz Inuwa has waded into the communal crisis rocking Evwreni Kingdom in Ughelli North Local Government Area of the State over the disbandment of the local vigilante team of the kingdom. Speaking at a stakeholder’sRead More The post Delta CP wades into Delta community crisis appeared first on Vanguard News. Full Article News Commissioner of Police delta Delta community crisis
community Osun community lauds Oyetola, Oyebamiji for restoring electricity In their area By hallmarknews.com Published On :: Fri, 08 May 2020 17:21:09 +0000 By Sunday Oguntuyi, Osogbo Residents of Ako community, Ikire in Irewole local government area of Osun state has expressed appreciation to Governor Adegboyega Oyetola and Commissioner for Finance, Mr Bola Oyebamiji for helping to restore electricity to the area after years of darkness. Speaking on behalf of the community, one of their leaders, Pastor Raphael […] The post Osun community lauds Oyetola, Oyebamiji for restoring electricity In their area appeared first on Hallmarknews. Full Article Energy Politics Gboyega Oyetola Osun State
community Maritime Varsity: Ugborodo Community Dumps Warri Kingdom, Supports Okerenkeko Location … Alleges Olu of Warri Trying To Cause Fresh Crisis By blanknewsonline.wordpress.com Published On :: Mon, 15 Jan 2018 12:37:23 +0000 Nigeria (Blank NEWS Online) –: The people of Ugborodo Community in Warri South West Local Government Area of Delta State have said that they are satisfied with the current location […] Full Article Breaking News News State Of The Nation Blank News
community Community Infrastructure Levy: Removal of Regulation 123 By www.eversheds.com Published On :: 2019-08-28 The Community Infrastructure Levy (Amendment) (England) (No. 2) Regulations 2019 are due to come into force on 1 September 2019. The 2019 Regulations were introduced by the Government earlier this year, alongside their response to the technical cons... Full Article
community Amid ruins, Ramazan meal reunites a community By feedproxy.google.com Published On :: Sat, 09 May 2020 06:00:00 +0500 ATAREB, Syria: Amid mounds of rubble and the ruins of buildings destroyed during nearly a decade of war, a Ramazan iftar meal has reunited a community in northwestern Syria, foreign media reported.Dozens of men and children sat out on a long line of blankets on the ground in the town of Atareb for... Full Article
community Community Q&A – Kelly Kearney By feedproxy.google.com Published On :: Tue, 04 Feb 2020 22:00:50 +0000 Kelly Kearney is a top contributor to MATLAB Answers and File Exchange. Here is a Q&A I did with Kelly who is a research scientist for the University of Washington in their Joint Institute for the Study of the Atmosphere and Ocean. ... read more >> Full Article Q & A
community Community Q&A – Kalyan Acharjya By feedproxy.google.com Published On :: Tue, 07 Apr 2020 17:48:50 +0000 Kalyan Acharjya has been contributing to MATLAB Answers since 2017. This past year his participation skyrocketed and he is currently a Rising Star. Here is a recent Q&A I had with Kalyan... read more >> Full Article Q & A
community Community Vegetable Farming for Livelihood Improvement - Additional Financing By www.adb.org Published On :: 2020-02-12 00:00:00 Approved project 50278-002 in Mongolia. Full Article
community Exploring Community-Based Financing Schemes to Finance Social Protection By www.adb.org Published On :: 2020-03-30 00:00:00 Social protection, especially health care systems for the poor, is essential to reduce inequality. Full Article
community HARMAN Embraces the Spirit of Community During COVID-19 By news.harman.com Published On :: Wed, 06 May 2020 14:00:00 GMT Helen Keller, author, lecturer, and political activist, once said, “Alone, we can do so little; together, we can do so much.” For over sixty years, HARMAN has demonstrated our passion for working together to support the communities where we live and work... Full Article
community Learning and Teaching Together to Advance Evidence-Based Clinical Education: A Faculty Learning Community By www.jdentaled.org Published On :: 2019-12-01T06:00:18-08:00 Clinical teaching is a cornerstone of health sciences education; it is also the most challenging aspect. The University of Pittsburgh Schools of Dental Medicine, Nursing, and Pharmacy developed a new evidence-based interprofessional course framed as a faculty learning community (FLC) around the principles of learning in a clinical environment. The aim of this study was to assess the overall effectiveness of this two-semester FLC at four health professions schools in academic year 2014-15. The assessment included anonymous participant surveys in each session and an anonymous end-of-course survey. Thirty-five faculty members from dental, health and rehabilitation sciences, nursing, and pharmacy enrolled in the FLC, with six to 32 enrollees attending each session. All attendees at each session completed the session evaluation surveys, but the attendance rate at each session ranged from 17.1% to 91.4%. Sixteen participants (46%) completed the end-of-course survey. The results showed overall positive responses to the FLC and changes in the participants’ self-reported knowledge. Session surveys showed that the participants found the FLC topics helpful and appreciated the opportunity to learn from each other and the interprofessional nature of the FLC. Responses to the end-of-course survey were in alignment with the individual session surveys and cited specific benefits as being the content, teaching materials, and structured discussions. In additional feedback, participants reported interest to continue as a cohort and to extend the peer-support system beyond the FLC. This outcomes assessment of the first round of the FLC confirmed that this cohort-based faculty development in an interprofessional setting was well received by its participants. Their feedback provided valuable insights for changes to future offerings. Full Article
community Linking Human Milk Oligosaccharides, Infant Fecal Community Types, and Later Risk To Require Antibiotics By mbio.asm.org Published On :: 2020-03-17T01:30:15-07:00 ABSTRACT Human milk oligosaccharides (HMOs) may provide health benefits to infants partly by shaping the development of the early-life intestinal microbiota. In a randomized double-blinded controlled multicentric clinical trial, healthy term infants received either infant formula (control) or the same formula with two HMOs (2'-fucosyllactose and lacto-N-neotetraose; test) from enrollment (0 to 14 days) to 6 months. Then, all infants received the same follow-up formula without HMOs until 12 months of age. Breastfed infants (BF) served as a reference group. Stool microbiota at 3 and 12 months, analyzed by 16S rRNA gene sequencing, clustered into seven fecal community types (FCTs) with marked differences in total microbial abundances. Three of the four 12-month FCTs were likely precursors of the adult enterotypes. At 3 months, microbiota composition in the test group (n = 58) appeared closer to that of BF (n = 35) than control (n = 63) by microbiota alpha (within group) and beta (between groups) diversity analyses and distribution of FCTs. While bifidobacteriaceae dominated two FCTs, its abundance was significantly higher in one (FCT BiH for Bifidobacteriaceae at high abundance) than in the other (FCT Bi for Bifidobacteriaceae). HMO supplementation increased the number of infants with FCT BiH (predominant in BF) at the expense of FCT Bi (predominant in control). We explored the association of the FCTs with reported morbidities and medication use up to 12 months. Formula-fed infants with FCT BiH at 3 months were significantly less likely to require antibiotics during the first year than those with FCT Bi. Previously reported lower rates of infection-related medication use with HMOs may therefore be linked to gut microbiota community types. (This study has been registered at ClinicalTrials.gov under registration number NCT01715246.) IMPORTANCE Human milk is the sole and recommended nutrition for the newborn infant and contains one of the largest constituents of diverse oligosaccharides, dubbed human milk oligosaccharides (HMOs). Preclinical and clinical association studies indicate that HMOs have multiple physiological functions largely mediated through the establishment of the gut microbiome. Until recently, HMOs were not available to investigate their role in randomized controlled intervention trials. To our knowledge, this is the first report on the effects of 2 HMOs on establishing microbiota in newborn infants. We provide a detailed description of the microbiota changes observed upon feeding a formula with 2 HMOs in comparison to breastfed reference infants' microbiota. Then, we associate the microbiota to long-term health as assessed by prescribed antibiotic use. Full Article
community Prokaryotic and Viral Community Composition of Freshwater Springs in Florida, USA By mbio.asm.org Published On :: 2020-04-07T01:31:16-07:00 ABSTRACT Aquifers, which are essential underground freshwater reservoirs worldwide, are understudied ecosystems that harbor diverse forms of microbial life. This study investigated the abundance and composition of prokaryotic and viral communities in the outflow of five springs across northern Florida, USA, as a proxy of microbial communities found in one of the most productive aquifers in the world, the Floridan aquifer. The average abundances of virus-like particles and prokaryotic cells were slightly lower than those reported from other groundwater systems, ranging from 9.6 x 103 ml–1 to 1.1 x 105 ml–1 and 2.2 x 103 ml–1 to 3.4 x 104 ml–1, respectively. Despite all of the springs being fed by the Floridan aquifer, sequencing of 16S rRNA genes and viral metagenomes (viromes) revealed unique communities in each spring, suggesting that groundwater microbial communities are influenced by land usage in recharge zones. The prokaryotic communities were dominated by Bacteria, and though the most abundant phyla (Proteobacteria, Cyanobacteria, and Bacteroidetes) were found in relatively high abundance across springs, variation was seen at finer taxonomic resolution. The viral sequences were most similar to those described from other aquatic environments. Sequencing resulted in the completion of 58 novel viral genomes representing members of the order Caudovirales as well as prokaryotic and eukaryotic single-stranded DNA (ssDNA) viruses. Sequences similar to those of ssDNA viruses were detected at all spring sites and dominated the identifiable sequences at one spring site, showing that these small viruses merit further investigation in groundwater systems. IMPORTANCE Aquifer systems may hold up to 40% of the total microbial biomass on Earth. However, little is known about the composition of microbial communities within these critical freshwater ecosystems. Here, we took advantage of Florida’s first-magnitude springs (the highest spring classification based on water discharge), each discharging at least 246 million liters of water each day from the Floridan aquifer system (FAS), to investigate prokaryotic and viral communities from the aquifer. The FAS serves as a major source of potable water in the Southeastern United States, providing water for large cities and citizens in three states. Unfortunately, the health of the FAS and its associated springs has declined in the past few decades due to nutrient loading, increased urbanization and agricultural activity in aquifer recharge zones, and saltwater intrusion. This is the first study to describe the prokaryotic and viral communities in Florida’s first-magnitude springs, providing a baseline against which to compare future ecosystem change. Full Article
community MtSSPdb: A New Database for the Small Secreted Peptide Research Community By www.plantphysiol.org Published On :: 2020-05-08T08:30:48-07:00 Full Article
community Differentiation of Community-Associated and Livestock-Associated Methicillin-Resistant Staphylococcus aureus Isolates and Identification of spa Types by Use of PCR and High-Resolution Melt Curve Analysis [Clinical Veterinary Microbiology] By jcm.asm.org Published On :: 2020-04-23T08:00:29-07:00 Infections due to methicillin-resistant Staphylococcus aureus (MRSA) are present worldwide and represent a major public health concern. The capability of PCR followed by high-resolution melt (HRM) curve analysis for the detection of community-associated and livestock-associated MRSA strains and the identification of staphylococcal protein A (spa) locus was evaluated in 74 MRSA samples which were isolated from the environment, humans, and pigs on a single piggery. PCR-HRM curve analysis identified four spa types among MRSA samples and differentiated MRSA strains accordingly. A nonsubjective differentiation model was developed according to genetic confidence percentage values produced by tested samples, which did not require visual interpretation of HRM curve results. The test was carried out at different settings, and result data were reanalyzed and confirmed with DNA sequencing. PCR-HRM curve analysis proved to be a robust and reliable test for spa typing and can be used as a tool in epidemiological studies. Full Article