Embodiments of the present invention are directed to a microcontroller device having a microprocessor, programmable memory components, and programmable analog and digital blocks. The programmable analog and digital blocks are configurable based on programming information stored in the memory components. Programmable interconnect logic, also programmable from the memory components, is used to couple the programmable analog and digital blocks as needed. The advanced microcontroller design also includes programmable input/output blocks for coupling selected signals to external pins. The memory components also include user programs that the embedded microprocessor executes. These programs may include instructions for programming the digital and analog blocks “on-the-fly,” e.g., dynamically. In one implementation, there are a plurality of programmable digital blocks and a plurality of programmable analog blocks.

A loading machine for feeding a receiver includes, but is not limited to, a drum cam that is configured to rotate. The drum cam includes, but is not limited to, a first cam path, and a second cam path. A rammer subassembly is positioned proximate the drum cam. The rammer subassembly includes, but is not limited to, a first member that is configured to engage the first cam path and to move longitudinally with respect to the drum cam upon a rotation of the drum cam, and a second member that is configured to telescopically engage the first member and further configured to engage the second cam path and telescopically move with respect to the first member upon the rotation of the drum cam.

A breaching device that may be used to create a linear and, if desired, continuous, cut or breach in a metal structure. The cut or breach created may be non-linear in shape and not deviate from the functionality of the device. The device includes a plurality of containers joined together, such as by a metal wire or the like to form a series of cutting charges. One or more of the containers includes Reactive Material (RM) that may be ignited electronically or some other activation mechanism. The containers that do contain RM are sealed with the RM therein and preferably fabricated to be sufficiently heat resistant so that the RM is only ignited intentionally. The RM that is contained in the containers may be fired simultaneously, sequentially or in a programmed pattern, depending on the requirements of the application.

A construction machine includes: a lower propelling body; and an upper slewing body which is slewably mounted on the lower propelling body. The upper slewing body includes: an engine room provided with an intake opening and a discharge opening; a duct provided in the engine room so as to communicate with the discharge opening; and an exhaust gas pipe which guides exhaust gas discharged from the engine into the duct. The discharge opening and an outlet of the duct are open downward from the engine room. A portion on a distal end side of the exhaust gas pipe is inserted into the duct. The duct and the exhaust gas pipe are configured to cause the exhaust gas to be mixed with cooled air in the duct and cause the mixture of the exhaust gas and the cooled air to be discharged downward from the discharge opening.

The present invention discloses a hierarchical composite material comprising a ferrous alloy reinforced with titanium carbides according to a defined geometry, in which said reinforced portion comprises an alternating macro-microstructure of millimetric areas concentrated with micrometric globular particles of titanium carbide separated by millimetric areas essentially free of micrometric globular particles of titanium carbide, said areas concentrated with micrometric globular particles of titanium carbide forming a microstructure in which the micrometric interstices between said globular particles are also filled by said ferrous alloy.

A method for leaching copper and molybdenum from an ore, residue and/or concentrate containing such, in which more than 1% w/w of the total molybdenum is present as a sulfide and in which more than 1% w/w of the total copper is present as an oxide, the method comprising the steps of: exposing the ore, residue and/or concentrate to an aqueous solution of chlorine (I)-based oxidizing species of a pH of at least 3.0; oxidizing the molybdenum by the chlorine-based oxidizing species thereby providing a treated ore, residue and/or concentrate and a reduced aqueous solution of chlorine-based oxidizing species; leaching the treated ore, residue and/or concentrate by exposing the treated ore, residue and/or concentrate to an aqueous ammoniacal ammonium carbonate solution to form a pregnant leach solution containing both copper and molybdenum; and passing the pregnant leach solution containing both copper and molybdenum to a means for metal recovery.

The invention provides a recycling step in an oxidative pressure leaching process for recovery of metals using halide ions, in which a portion of the leached solids are recycled back to the feed to the autoclave, to allow two or more passes through the high temperature leaching step.

Tire changing machines having force feedback and position control systems are described that may be used to control operation of one or more components of the machine during a tire change procedure. Information may be provided to a machine operator concerning detected operating conditions during the procedure. Excessive or abnormal forces or component positions can be detected and corrective action may be taken at any point in the tire change procedure. Successful component position profiles may be saved for future recall and execution by the machine.

The tire run-flat ring removal and installation machine includes three basic embodiments, each having a tire support table (14) and actuators for removing and/or reinstalling the run-flat ring (R) in the tire (T). The actuators may be hydraulically, pneumatically, or electromechanically powered, and save the technician considerable time and effort in the process.

A tire-changing machine comprising support means (4) for a wheel, a tool-bearing arm (50) at an end of which a bead-breaking tool (6) is hinged according to a hinge axis (E) which is perpendicular to an axis (A) of the wheel, and first motorised means (32, 33) for causing a relative movement, in a parallel direction to the axis (A) of the wheel, between the support means (4) and the tool-bearing arm (50), such as to press the bead-breaking tool (6) against a flank of a tire on the wheel, characterized in that it comprises second motorised means (64), activatable independently from the first motorised means (32, 33), which second motorised means (64) are destined to rotate the bead-breaking tool (6) about the hinge axis (E) with the tool-bearing arm (50), in order to vary an inclination of the bead-breaking tool (6) with respect to the axis (A) of the wheel which is mounted on the support means (4).

A working tool orienting device in a tire demounting machine includes a base which supports a rotary apparatus, rotating about an axis of rotation and designed to bear and lock wheel rims thereon; a column rising from the base and having an end secured to the base and an end opposite thereto facing upward; a moving arm, which is moved between positions close to and away from the rotary apparatus; a support arrangement for supporting working tools, which is coupled with the upward facing end, and joint means interposed between the support arrangement and the upward facing end, which are configured to make the support arrangement movable relative to the arm.

A device for automatic demounting of a tire from a rim having: a support arm (4); a demounting tool (10) articulated to the support arm (4); an attachment member (12) having one end articulated to the demounting tool (10) by a first articulation pin (13) and the other end thereof pivoted to the support arm (4) around a second articulation pin (14) extending substantially parallel to the first articulation pin (13); at least one extension appendage of the support arm (4) or a section (17) of the support arm (4), which extends beyond the second articulation pin (14); and at least one tie rod member (18) that is articulated on one side to the tool, and on the other side to the extension appendage or section (17) of the support arm (4).

Tire changing machines with automated positioning and closed loop control of bead pressing devices are described to maintain and control operation of the bead pressing devices during tire mount and de-mount procedures. Methodology is also disclosed.

A bead breaking unit for tire changing machines includes an arm having a bead breaking tool and associated rotatable with a supporting structure between an away position, wherein the tool is spaced out from the supporting structure, and a work position, wherein the tool is near the supporting structure, an actuator apparatus having a mobile element associated sliding with the arm, a dragging device for dragging the arm, associated with the mobile element and suitable for operating during movement of the mobile element from an extracted position to a retracted position to bring the arm to the work position, a coupling device between the mobile element and the arm, suitable for operating during movement of the mobile element from the retracted to the extracted position to bring the arm from the work position to the away position, and a selection device associated with, and suitable for operating/disconnecting the coupling device.

There is described a machine (1) for regeneration of pneumatic tires (2) comprising a beading device (5) for axial locking of the pneumatic tire to be regenerated in the direction of the revolving axis of the pneumatic tire (2) which provides an untranslatable bead (6) and a translatable bead (7) in said direction, a work rasp (9) mounted on a spindle (25), inflation means (66) of the pneumatic tire and a discharge cochlea (77). Said machine further comprises a roughing miller (8) suitable to prepare the pneumatic tire to a subsequent finishing by means of the rasp (9), said miller (8) being mounted on the same spindle (25) of the rasp (9) and after it, and constituted of a monoblock (27) with discharge channels (28) for chips produced by inserts (29) with a curved profile (30) providing a plurality of planar blades (31) joined by discharge grooves (32) of the chips produced.

A machine for fitting and removing wheel tires for vehicles includes a bearing structure, at least an apparatus for fastening and rotating the rim of a wheel for vehicles, a tool-carrier unit associated mobile with the bearing structure and having a removal tool, at least an adjustment apparatus suitable for adjusting the position of the tool-carrier unit with respect to the bearing structure, to place the tool-carrier unit into a work configuration wherein the removal tool is arranged in proximity of the apparatus for fastening and rotating, at least a memorisation device for memorising the position of the tool-carrier unit in the work configuration and at least a positioning device for positioning the tool-carrier unit with respect to the bearing structure between the work configuration and a configuration of minimum overall dimensions, wherein the tool-carrier unit is arranged in proximity of the bearing structure.

The present invention relates to a device for demounting a tire (T) from a rim (W) including at least one support element;an articulation pin element borne by the at least one support element;a spring shackle element having a first end and a second end articulated on the articulation pin;a demounting lever;an actuator borne by the at least one support element and set to act on the demounting lever in order to make the demounting lever angularly move during use; andfriction or elastic loading means designed to slow the angular movement of the spring shackle element around the articulation pin with respect to its angular movement around the first axis (x-x).

A tire changer machine and tire demounting methodology includes a demount tool assembly having a demount tool selectively positionable relative to a support and configured to extract a bead of the tire from the wheel rim. A guide element is mounted to the support, and the guide element is operable to provide a limited degree of freedom of the distal end from a predetermined path of motion in a tire demount procedure, thereby allowing the distal end to reliably grip the tire bead while the support arm is maintained in a stationary position relative to the wheel rim.

Automated tire changer machines, control systems and methods therefor utilize positive contact and contact force generation between wheel rims and bead breaker devices to break tire bead seals. The bead breaker devices may accordingly follow different geometries of wheel rims without prior knowledge or identification of the specific rim configuration by the machine.

The present invention concerns a wheel-clamping device for wheels on wheel-service-machines with only one drive unit and further a method for clamping wheels on a wheel-clamping device of wheel-service-machines with only one drive unit. The innovative wheel-clamping device for a wheel-service-machine according to the present invention comprises a frame 20 having a through-opening 22, and a spindle 30 being rotatably supported in the through-opening 22. Further, the spindle 30 has a through-hole 32 with a mounting-side end 30b and a driving-side end 30a, which is connectable to a drive means which is provided for a rotary movement for the spindle 30, wherein the spindle 30 has an external thread 34 on an external circumferential portion. Moreover, the wheel-clamping device comprises a sleeve 50 with a turntable 58 for the wheel to be clamped, wherein the sleeve 50 has an internal thread portion 52 which is in threaded engagement with the external thread-portion 34 of the spindle 30. Furthermore, a stopping or holding means 70 is able to temporarily hold the sleeve 50, and a clamping means 60 for the temporarily fixation of a fixing element 40 which is inserted through the mounting-side end 30b, to the spindle 30 is also provided in the innovative wheel-clamping device.

Provided is bearing steel excellent in workability after spheroidizing-annealing and in hydrogen fatigue resistance property after quenching and tempering. The bearing steel has a chemical composition containing, by mass %: 0.85% to 1.10% C; 0.30% to 0.80% Si; 0.90% to 2.00% Mn; 0.025% or less P; 0.02% or less S; 0.05% or less Al; 1.8% to 2.5% Cr; 0.15% to 0.4% Mo; 0.0080% or less N; and 0.0020% or less O, which further contains more than 0.0015% to 0.0050% or less Sb, with the balance being Fe and incidental impurities, to thereby effectively suppress the generation of WEA even in environment where hydrogen penetrates into the steel, so as to improve the roiling contact fatigue life and also the workability such as cuttability and forgeability of the material.

A fluid screen for a washing machine, and a washing machine including a fluid screen, is provided. The fluid screen includes a generally planar member including a first end and a second end extending from the first end. The first end is mounted within the wash tank of the washing machine at a location generally in proximity to an operating fluid level within the wash tank. During operation of the washing machine, fluid circulates within the wash tank to create a rolling wash action and the second end of the fluid screen extends from the first end generally in the direction of fluid flow across the surface of the wash tank.

Embodiments of the present disclosure include cleaning processes, closed loop cleaning machines, and methods of cleaning an article. The cleaning process includes contacting a surface of an article with a cleaning composition in a cleaning chamber, where the cleaning composition includes at least about 85 percent by weight organic solvents, based on total weight of the cleaning composition, and where at least about 5 percent by weight of the organic solvents is propylene glycol, based on total weight of the organic solvents, to clean the surface of the article, collecting the cleaning composition including contaminants, and recovering the cleaning composition via distillation, where a distillation apparatus removes the contaminants from the cleaning composition and is connected to the cleaning chamber by a process stream.

A dishwasher having a washing compartment; an air-guiding channel to generate an airflow; and a sorption drying system to dry items to be washed. The sorption drying system has a sorption compartment with reversibly dehydratable sorption material and the sorption compartment is connected to the washing compartment by the air-guiding channel. The reversibly dehydratable sorption material in the sorption compartment is layered in form of a sorption unit that has an inlet cross-sectional area such that the substantially identical respective air volume flow value that is applied to each entry point of the inlet cross-sectional area.

A cleaning machine for containers, containing a housing in which various treatment stations, including a feed station and a discharge station, are accommodated, whereby the housing has two side walls located opposite each other. In order to simplify the installation of the cleaning machine, a modular construction is used, whereby at least the discharge station is formed as a module.

The invention relates to a cleaning system for coffee machines, comprising a cleaning device for periodically cleaning the coffee- and/or milk-carrying components using a cleaning liquid, which contains cleaning agent in the form of balls or tablets dissolved therein, wherein the cleaning device is provided with a time- and/or product-cycle-dependent controller and a metering apparatus (1) for the cleaning agent (10) that interacts with the controller. Thus, it is possible to program the cleaning process in a customized manner according to the mode of operation and to carry out the cleaning process fully automatically according to the program. The metering of the cleaning agent (10) according to the program ensures an always optimal cleaning effect together with sparing use of cleaning agents. Simple and safe handling is achieved by using ball- or tablet-shaped cleaning agents.

In a method for multiple cutoff machining a rare earth magnet block, a cutting fluid feed nozzle having a plurality of slits is combined with a plurality of cutoff abrasive blades coaxially mounted on a rotating shaft, each said blade comprising a base disk and a peripheral cutting part. The slits in the feed nozzle into which the outer peripheral portions of cutoff abrasive blades are inserted serve to restrict any axial run-out of the cutoff abrasive blades during rotation. Cutting fluid is fed from the feed nozzle through slits to the rotating cutoff abrasive blades and eventually to points of cutoff machining on the magnet block.

Provided is a gear grinding machine the maintainability of which can be improved and which is capable of being miniaturized. More specifically, provided is a gear grinding machine wherein a threaded grinding stone (17) and a workpiece (W) are engaged with each other and are rotated in this state, resulting in the workpiece (W) being ground, and wherein between a dressing position (P2) where the threaded grinding stone (17) can be dressed and a retreat position (P1) to which a retreat is made from the dressing position (P2) during grinding, a disk dresser (66) which, by being driven into rotation, dresses the threaded grinding stone (17) is swung about an axis (C2) which is parallel to an axis (C1) that is the rotational axis for the workpiece (W) and which intersects, at right angles, the direction in which the threaded grinding stone (17) is fed toward the workpiece (W).

A method of ductile mode machining a component of a plasma processing apparatus wherein the component is made of nonmetallic hard and brittle material wherein the method comprises single point turning the component with a diamond cutting tool causing a portion of the nonmetallic hard and brittle material to undergo a high pressure phase transformation to form a ductile phase portion of the hard and brittle material during chip formation wherein a turned surface is formed from a phase changed material and the turned surface is a grooved textured surface of phase changed material.

The core sand filling device includes the core box, a blow head which is placed below the core box so as to move up and down in a relative manner to the core box and divided into a sand blowing chamber and a sand storage chamber that are communicatively connected to each other, a compressed air supply unit which is communicatively connected to the sand storage chamber and supplies compressed air into the sand storage chamber, an aeration air supply unit which is communicatively connected to the sand blowing chamber and supplies into the sand blowing chamber aeration air for suspending and fluidizing core sand inside the sand blowing chamber, and an exhaust valve which is communicatively connected to the sand blowing chamber and exhausts compressed air remaining in the sand blowing chamber.

Provided in one embodiment is a method of forming a connection mechanism in or on a bulk-solidifying amorphous alloy by casting in or on, or forming with the bulk-solidifying amorphous alloy, a machinable metal. The connection mechanism can be formed by machining the machinable metal.

A dial of a circular hosiery knitting machine of the type with a cylinder and dial with yarn cutting device. The dial comprises a dial body which is substantially disk-shaped. The dial is provided with a cutting device, which comprises a plurality of cutting sectors which are distributed around the axis of the dial body and have cutting edges. The cutting sectors are arranged at sectors of the dial body which are intended to be free from needles. The cutting device comprises a cutter which is adapted to abut against the cutting edges of the cutting sectors to cut at least one yarn engaged by one of the cutting sectors in its rotary motion about the axis of the dial body with respect to the cutter.

A rotary sinker enabling stitch formation by a knitting machine using a rotor for forming a stitch, a knitting machine including the rotary sinker, and a stitch forming method are provided. The ring sinkers as rotating bodies are capable of rotating about a rotation axis independently of each other. The rotation times of the ring sinkers are set to be different from each other. Thus, the rotation times are adjusted to be different between the ring sinker that holds an old loop and the ring sinker that holds a new loop. Adjustment of the rotation times prevents application of a large tension to the knitting yarn made of low stretch fiber, and a stitch can be formed even with a knitting yarn made of non-stretch or low-stretch fiber.

A double-cylinder circular machine, particularly for knitting hosiery items, with simplified actuation mechanism, comprising a supporting structure which comprises a footing, which supports a lower needle cylinder, and a column which extends substantially vertically, protrudes upwardly from the footing and supports an upper needle cylinder, which is arranged above and coaxially with respect to the lower needle cylinder, and further comprising elements for the actuation of the lower needle cylinder and of the upper needle cylinder with a rotary motion about a common axis, the actuation elements comprising an electric motor which is connected kinematically to the lower needle cylinder and to the upper needle cylinder and is accommodated inside the column.

A knitting machine comprising a needle holder, which supports a plurality of needles which can be actuated with an alternating motion along their axis and means for guiding the needles on the needle holder which comprise channels for forming knitting which are defined proximate to the end of the needle holder and sliding channels which are defined on the needle holder in a region that is spaced from the end of the needle holder, each one of the channels for forming knitting being engageable by a needle and defining with its inlet, which is directed toward the outside of the needle holder, resting contact regions for the knitting during the retracting motion of the needles, the number of the sliding channels being smaller than the number of the channels for forming knitting.

A knitting head of a knitting machine for manufacturing flexible hoses (1) with a knitted reinforcement layer, which comprises a guide body (14) with a central tubular conduit (15) for the passage of the bearing layer (2) and having a outlet end portion (16) with a first outer peripheral surface (17) having a first predetermined radius (r1), a plurality of knitting needles (18) arranged along the periphery of said end portion (16) of said conduit (15) and having first longitudinal end sections (18') arranged along the circumference of said outer peripheral surface (17) and second longitudinal end sections (18″) arranged over a second cylindrical surface (19) having a second radius (r2) greater than said first radius (r1), at least one disk-shaped cam element (22), which is adapted to rotate at a first rotation speed and has a third predetermined radius (r3). The third radius (r3) is greater than said second radius (r2), connection means (23) being provided for connecting said disk-shaped element (22) to said second end sections (18″) of said needles (18), said connection means (23) having at least one longitudinal portion (24) with a radial dimension decreasing from said third radius (r3) to said second radius (r2).

A knitting machine includes a take-down assembly that includes a first take-down roller and a second take-down roller. The first take-down roller is configured to rotatably contact and apply tension to a first portion of a knit component. The second take-down roller is configured to rotatably contact and apply tension to a second portion of the knit component. The knitting machine further includes a first actuator that actuates to selectively adjust tension applied by the first take-down roller on the first portion of the knit component. Furthermore, the knitting machine includes a second actuator that actuates to selectively adjust tension applied by the second take-down roller on the second portion of the knit component. Additionally, the knitting machine includes a controller that is operably coupled to the first actuator and the second actuator to selectively and independently control actuation of the first actuator and the second actuator.

Sinker cam segments in a circular knitting machine are located with respect to the generally radial direction by a generally radially inwardly facing location surface disposed radially outward of the segments. Each segment's radially outer end defines a contact region that abuts the locating surface. The contact region can be complementary surface contour to that of the locating surface, or a pair of discrete, spaced contact points protruding out from the radially outer end of the segment. The contact region is a reference for positioning the sinker cam segment with respect to the generally radial direction.

A knitting machine having needles which are mounted to be longitudinally moveable and having needle cams (14) with a needle control curve (13) for moving the needles, the needle control curves (13), at least in the latch closure region (Z), having a withdrawal angle (α)≦35°.

A knitting machine includes a take-down assembly that includes a first take-down roller and a second take-down roller. The first take-down roller is configured to rotatably contact and apply tension to a first portion of a knit component. The second take-down roller is configured to rotatably contact and apply tension to a second portion of the knit component. The knitting machine further includes a first actuator that actuates to selectively adjust tension applied by the first take-down roller on the first portion of the knit component. Furthermore, the knitting machine includes a second actuator that actuates to selectively adjust tension applied by the second take-down roller on the second portion of the knit component. Additionally, the knitting machine includes a controller that is operably coupled to the first actuator and the second actuator to selectively and independently control actuation of the first actuator and the second actuator.

In the case of a single circular knitting machine consisting of a central rotatable needle cylinder (Z), around which a sinker ring (PR) comprising sinkers (P) as well as stationary cam systems (S) are arranged, which act on the needles (1), which are in each case assigned to the sinkers (P) and which can be moved vertically up and down, so as to replace the pitches, which hit one another rigidly, with a sinker grid system, which is flexible per se, which is automatically oriented in the needle gaps, a rocker (44) comprising an upper and a lower control bump (45) being provided in each case on the end of the sinkers (P), which is spaced apart from the needle, the sinker ring (PR), at the end below the sinkers (P), which is spaced apart from the needle, is embodied as a pivot point projection (40) comprising pivot point slits (41), in which the sinkers (P) are accommodated with their pivot inlet (43) so that they are capable of being tilted and the sinkers (P) being are laterally fixed in the needle gaps with sliding noses (47) at the end, which is spaced apart from the needle, transport the last knitting loops to the needle shaft (1) behind the needle latches in response to the knitting loop formation.

A machine for the homogenization and thermal treatment of liquid and semi-liquid food products, for example ice creams, whipped cream, creams, chocolate, yogurt and the like, comprises a containment tank for the mixture and a centrifugal pump put in fluid communication with the bottom of the containment tank for drawing mixture from the tank and putting it back into the tank, heating and cooling means acting at the pump for heating and cooling the mixture in transit in the pump. The heating and cooling means comprise a thermal machine with reversible thermodynamic cycle and using carbon dioxide as refrigerant.

Machine (1) for extracting puree, or fruit juice starting from vegetable or animal food, such as a rough extractor or a fine extractor. The machine (1) comprises essentially a cylindrical body (2) having an inlet (3) for the product to treat, an outlet (4) for a part of the product that can be used and an outlet (5) for another part of product to dispose of. The rotor (6) is wheeled to a motor (80) by a shaft (15). The product that is put in the cylindrical body (2) of the machine (1) is pushed by centrifugal force by a plurality of blades (20) of the rotor (6) to pass through a sieve (7) having holes (8) and a cylindrical or conical shape. The blades (20) of the rotor (6) comprise, in particular a first portion (21) having a first concavity and a second portion (22) having a second concavity opposite to the first.

The present invention relates to a device to regulate the level of a liquid inside a boiler of a coffee machine, having a transparent duct connected to the boiler and designed to visibly display the level of the liquid inside the boiler, a capacitive sensor that can be positioned outside the transparent duct in determined positions and designed to generate electric signals representing the presence or absence of liquid in correspondence to the position of the sensor along the transparent duct. The sensor of the device has first measuring elements and second measuring elements, both of capacitive type; the second measuring elements are positioned at a predetermined distance from the first measuring elements, and a measuring device designed to measure the impedance between the first and second measuring elements and to generate signals indicating the presence or absence of liquid in correspondence to the position of the sensor. The invention also relates to the machine that includes this device.

A coffee machine comprises a frothing device, wherein an internal milk channel leading to a frothing chamber is provided into which opens a bypass air inlet for operable air supply to the frothing device. A flexible milk suction line connects the frothing device with a milk supply container. Means are provided for cleaning the frothing device and the milk suction line with rinsing water from a continuous-flow water heater of the coffee machine. A controlled valve arrangement is provided which feeds rinsing water from the continuous-flow water heater to the bypass air inlet of the frothing device. A milk suction end of the milk suction line is directly or indirectly, fluid-conductively connectable with a residual water pan of the coffee machine prior to feeding rinsing water to the bypass air inlet.

Provided are methods of generating chimeric antigen receptors (CAR). In some embodiments, library screening of CAR is performed by generating a vector encoding the CAR from random attachment of vectors from libraries of vectors encoding antigen-binding domains (e.g., scFv regions), hinge regions, and endodomains. In some embodiments, the vectors contain a transposon.

The present invention relates to Chimeric Antigen Receptors (CAR) that are recombinant chimeric proteins able to redirect immune cell specificity and reactivity toward selected membrane antigens, and more particularly in which extracellular ligand binding is a scFV derived from a CD123 monoclonal antibody, conferring specific immunity against CD123 positive cells. The engineered immune cells endowed with such CARs are particularly suited for treating lymphomas and leukemia.

The invention provides compositions and methods for treating leukemia, for example, acute myeloid leukemia (AML) and B-cell acute lymphoid leukemia (B-ALL). The invention also relates to at least one chimeric antigen receptor (CAR) specific to CD123, vectors comprising the same, and recombinant T cells comprising the CD123 CAR. The invention also includes methods of administering a genetically modified T cell expressing a CAR that comprises a CD123 binding domain. The invention also includes methods of bone marrow ablation for use in treatments necessitating bone marrow reconditioning or transplant.

The present invention relates to Chimeric Antigen Receptors (CAR) that are recombinant chimeric proteins able to redirect immune cell specificity and reactivity toward selected membrane antigens, and more particularly in which extracellular ligand binding is a scFV derived from a BCMA monoclonal antibody, conferring specific immunity against BCMA positive cells. The engineered immune cells endowed with such CARs are particularly suited for treating lymphomas, multiple myeloma and leukemia.