A new paper analyzes three decades of fatalities around the world and predicts how "superbugs" will affect human health in the future

Most adult king penguins weigh between 31 and 37 pounds. At nine months old, a 51.8-pound Pesto is already looming over his parents

The wild canines have the same muscles used by domestic dogs to create the wide-eyed, pleading look that captures humans' hearts

Scientists converted the patient’s own cells into blood sugar-regulating cell clusters before injecting them back into her abdomen—and one year later, she still doesn't need insulin injections

Milagra, Spanish for “miracle,” was hatched and raised in captivity by foster condor parents after her mother died of avian flu in April 2023

Scientists used EEG headsets, MRI machines and eye trackers to study volunteers' responses to five paintings housed at the Mauritshuis museum in the Netherlands

This week's supermoon is closer to Earth than any other in 2024

Using advanced imaging techniques, researchers discovered modern glue, drill holes and even a fragment of a drill bit in the pastiches

A scientist used at-home experiments to test whether cats hesitated when moving through increasingly shorter or narrower openings

Government corruption, loved ones becoming ill or dying, cyberterrorism and nuclear weapons topped the list of Chapman University's annual survey

Unlike other birds, which get pigments from their diets, parrots produce their own—but scientists never fully understood the underlying mechanisms, until now

Born October 30 to parents Gloria and Otto at the Edinburgh Zoo in Scotland, the hippo is already gaining popularity, following in the footsteps of viral sensation Moo Deng

Mary, a 54-year-old Asian elephant at the Berlin Zoo, is the “queen of showering,” but her companion Anchali seems to have figured out how to exploit that habit to play pranks

Laboratory study of natural populations of mosquitoes can play a key role in determining the underlying causes of variation in burdens of mosquito-borne disease. Aedes aegypti is the main vector of the viruses that cause dengue, chikungunya, Zika, and yellow fever, making it a high priority for laboratory study. Ae. aegypti eggs provide an ideal starting point for new laboratory colonies. Eggs can be collected using ovicups, which are small plastic cups lined with seed-germination paper and partially filled with leaf-infused H₂O. Once collected, dry eggs will remain viable for months and can be safely transported long distances back to the laboratory as long as they are properly stored. This protocol provides step-by-step instructions for preparing for collecting, storing, and hatching Ae. aegypti eggs and has successfully yielded laboratory colonies from locations across both the native and invasive range of this species.

The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

The ancient burial structures, strikingly similar all across Europe and the mediterranean, have puzzled scientists and historians for centuries

An opioid replacement program in downtown Fredericton will continue to operate at the River Stone Recovery Centre until March 2025 after it got a one-year extension from Health Canada, despite increased opposition to the clinic.

Even as they fundraise for other community organizations, some legions across Canada are having trouble keeping their own lights on amid rising inflation and maintenance costs.

The mayor of the eastern P.E.I. town of Three Rivers says comments on social media have become so 'aggressive' that council has decided to hold Tuesday night’s meeting virtually instead of in-person out of concerns for their own safety.

CBC Sports sat down with Hockey Hall of Famer Mats Sundin, showing him video of some of the biggest moments of his career.

Hired in July by the Seattle Kraken as an assistant coach after two seasons with their AHL affiliate, Jessica Campbell is starting to get to know the team, schedule demands and managing "the vigorous push through it all." It's also about managing the attention as a trailblazer.

The Edmonton Oilers may have found the break they were looking for. After struggling through the first month of the NHL season, the Oilers trounced the Vancouver Canucks 7-3 on Saturday.

Ukraine attacked Moscow on Sunday with at least 34 drones, the biggest drone strike on the Russian capital since the start of the war in 2022, forcing flights to be diverted from three of the city's major airports and injuring at least one person.

Anautogenous female mosquitoes, which ingest a blood meal from warm-blooded vertebrates to produce eggs, have become a valuable model organism for investigating signaling pathways and physiological processes that occur during egg development. Different molecular pathways tightly regulate the initiation of egg development and are governed by a balance among different insect hormones. Gravid (mature egg-carrying) females deposit fully developed eggs at the end of each gonotrophic cycle, which is defined as the time interval between the ingestion of a blood meal to oviposition. An intact eggshell protects the oocyte and embryo inside from external factors such as desiccation, physical damage, etc., and the various eggshell proteins are spatially and temporary deposited during oogenesis. Additionally, follicle resorption (oosorption) during blood meal–induced mosquito ovarian follicle development is an adapted physiological process that optimizes reproductive fitness. Mosquito oocytes grow and mature synchronously throughout oogenesis; however, during the later stages of oogenesis, some oocytes may undergo oosorption if sufficient nutrients are unavailable. This introduction highlights how mosquito egg development can be used to investigate follicular resorption and identify proteins involved in eggshell formation in Aedes aegypti mosquitoes.

DWGgateway plug-in lets AutoCAD users share designs with virtually anyone

More than 100,000 AutoCAD software users download free compatibility solution from SolidWorks Corporation

-- Crowdsourced video design series produces stroller that makes dads proud --

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report

Terry Roggensack, The Hightower Report