The Delaware Department of Justice’s Human Trafficking Unit has indicted three individuals with running a ring of illicit massage establishments, Attorney General Kathy Jennings announced today   “Some people assume human trafficking only happens on the other side of the world, but the reality is that it is right in our backyard — and cases […]

Child Trafficking Racket: অভিযুক্তদের কাছ থেকে ২ দিনের এক শিশুকন্যাকে উদ্ধার করা হয়েছে। তাকে উদ্ধার করে হাওড়া হাসপাতালে পাঠানো হয়েছে। জানা গিয়েছে পাটনা থেকে পাচার করে আনা হয় ওই শিশুটিকে

Child Trafficking Racket: আরও জানা যায়, ৪ লক্ষ টাকার বিনিময়ে শিশু দেওয়ার কথা ছিল। সিআইডির ২ অফিসার দম্পত্তি সেজে ওই শিশুকে নেওয়ার জন্য আজ হাজির হন শালিমার স্টেশন এলাকায়। সেখানেই সিআইডির হাতে ধরা পড়েন ঠাকুর পুকুরের বাসিন্দা মকুল সরকার ও মানিক হালদার।  

Photojournalist Smita Sharma’s book ‘We Cry in Silence’ lays bare the patriarchy that makes girls an easy target for traffickers

The coordinator of OM Italy’s anti-trafficking ministry describes the birth of the ministry through meeting a Nigerian woman forced into prostitution in Rome.

Participating in a Transform outreach to trafficked women gave OMer Erika Tello the vision of founding the OM Italy anti-trafficking ministry.

The anti-trafficking efforts by OM Italia are developing as they work to involve the local church in this outreach.

In the summer of 2013, a South African member of OM Czech Republic returned to her home country to lead anti-trafficking training and awareness seminars.

Human trafficking grows like a cancer in Ecuador. Boris and Fernanda Salinas are destined to fight it.

“I climb in the name of the young women we serve,” said Boris Salinas, who will participate in a Freedom Climb event on 26 April.

A recent incident where a Hong Kong citizen forced his maid to travel abroad with him has opened up a wide discussion on the issue of human trafficking. The outcome of the incident has seen a rather curious scenario develop, wherein Hong Kong holidaymakers…

Osiris Martinez-Guzman, Mathilda M. Willoughby, Arushi Saini, Jonathan V. Dietz, Iryna Bohovych, Amy E. Medlock, Oleh Khalimonchuk, and Amit R. Reddi

Heme is a cofactor and signaling molecule that is essential for much of aerobic life. All heme-dependent processes in eukaryotes require that heme is trafficked from its site of synthesis in the mitochondria to hemoproteins located throughout the cell. However, the mechanisms governing the mobilization of heme out of the mitochondria, and the spatio-temporal dynamics of these processes, are poorly understood. Herein, using genetically encoded fluorescent heme sensors, we developed a live cell assay to monitor heme distribution dynamics between the mitochondrial inner-membrane, where heme is synthesized, and the mitochondrial matrix, cytosol, and nucleus. Surprisingly, heme trafficking to the nucleus is ~25% faster than to the cytosol or mitochondrial matrix, which are nearly identical, potentially supporting a role for heme as a mitochondrial-nuclear retrograde signal. Moreover, we discovered that the heme synthetic enzyme, 5-aminolevulinic acid synthase (ALAS), and GTPases in control of the mitochondrial dynamics machinery, Mgm1 and Dnm1, and ER contact sites, Gem1, regulate the flow of heme between the mitochondria and nucleus. Overall, our results indicate that there are parallel pathways for the distribution of bioavailable heme.

Vaishnavi Ananthanarayanan
Apr 17, 2020; 133:jcs245928-jcs245928
Meeting Report

Monica Deller, an Amazon Bestselling author, hopes to bring more awareness to the issue of human trafficking and is compiling stories for a new book series on human trafficking and related subjects.

Ribbon Cutting Ceremony Launches First Community for Sex Trafficking Survivors

Sex Trafficking Survivors in North Texas Find Individualized Care in a Safe Haven

Actress and Reality TV Star Tiffany Panhilason Hosts A Powerful Yoga Session For Mentari USA's Human Trafficking Survivor Empowerment Program

Actress and Reality TV Star Tiffany Panhilason Hosts Thanksgiving Meal For Mentari USA's Human Trafficking Survivors

Monica Deller, an Amazon Bestselling author, hopes to bring more awareness to the issue of human trafficking and is compiling stories for a new book series on human trafficking and related subjects.

The initiative features a revolutionary 3-day workshop designed to help organizations break down barriers, locate and advocate for victims, and deter demand.

Former President of Venezuela Nicolás Maduro Moros, Venezuela’s vice president for the economy, Venezuela’s Minister of Defense, and Venezuela’s Chief Supreme Court Justice are among those charged in New York City; Washington, DC; and Miami, along with current and former Venezuelan government officials as well as two Fuerzas Armadas Revolucionarias de Colombia (FARC) leaders, announced U.S. Attorney General William P. Barr, U.S. Attorney Geoffrey S. Berman of the Southern District of New York, U.S. Attorney Ariana Fajardo Orshan of the Southern District of Florida, Assistant Attorney General Brian A. Benczkowski of the Justice Department’s Criminal Division, Acting Administrator Uttam Dhillon of the U.S. Drug Enforcement Administration (DEA) and Acting Executive Associate Director Alysa D. Erichs of U.S. Immigration and Customs Enforcement’s Homeland Security Investigations (HSI).

I am a longtime resident of Johnson County, currently in my 25th year of law enforcement. I worked for the Coralville Police Department in the late 1990’s and transferred to the Cedar Rapids Police Department in 1999 where I am a sergeant of the patrol division. I have degrees in criminal justice and organizational leadership and have advanced leadership training from Northwestern University in the School of Police Staff and Command.

Working in the second-largest city in the state has offered me many opportunities to lead. I have taught in the police academy and defensive tactics and as a field training instructor. I was the director of the Eastern Iowa Heroin Initiative, where I founded CRUSH of Iowa (Community Resources United to Stop Heroin). CRUSH is a community-based, grassroots organization helping all those affected by substance abuse disorder.

My passion has been community outreach. Currently I am a member of the Johnson County Human Trafficking Coalition and the Johnson County Prevention Partnership. Through these partnerships I will create a criminal interdiction team to fight the trafficking of humans, weapons and narcotics.

As sheriff, my top three concerns are substance abuse, mental health and human trafficking. I believe in creating long-lasting relationships with the community. I believe in common sense solutions without the haze of political bias. I believe that every citizen has a voice and should be heard. I believe in building a proactive and progressive law enforcement agency that serves with professionalism, compassion and dedication to the citizens. I believe we need to place the community back into community policing.

I am ready to be sheriff of Johnson County. I am a proven leader who is determined to build bridges with the citizens of the county and lead with accountability, trust and transparency. I will fight for all of Johnson County as sheriff because I have done so all of my life. This election is not just about me, it is about us. We, together, will make a positive impact on Johnson County. The status quo is not working. It’s time for change!

I would love to have your vote on June 2. We work better when we work together. People before politics!

Al Fear is a candidate in the Democratic primary for Johnson County sheriff. alfearforsheriff.com

Federal, state and local law enforcement officials in New York announced the dismantling of a gun trafficking ring on Long Island.

The New Yorker contributor Jenna Krajeski recently met with a woman who calls herself Esperanza. In her home country, Esperanza was coerced and threatened into prostitution, and later was trafficked into the United States, where she was subjected to appalling conditions. Esperanza eventually obtained legal help, and applied for something called a T visa. The T visa contains unusual provisions that recognize the unique circumstances of human-trafficking victims in seeking legal status. It has also been a crucial tool to obtaining victims’ coöperation in prosecuting traffickers. The Trump Administration claims to want to fight the problem of human trafficking, but Krajeski notes that its policies have done the opposite: T-visa applicants can now be deported if their applications are rejected. This dramatic change in policy sharply reduced the number of applications from victims seeking help. “If what [the Administration] cares about is putting traffickers in prison, which is what they say they care about, their prosecutions are going down and will go down further,” Martina Vandenberg, the president of the Human Trafficking Legal Center, says. “Trafficking victims under the circumstances can’t actually coöperate.”

An outreach worker linked to a medically supervised safe injecting room in Melbourne's inner east is among a group of people now charged with trafficking the drug heroin.

The head of a community centre which houses Melbourne's controversial safe injecting centre is stood down after several staff linked to other services at the centre have been charged with trafficking heroin.

A 27-year-old Canberra man is found guilty of running a major drug operation to fuel a flamboyant lifestyle complete with expensive cars and inspirational quotes played out on Facebook and Instagram.

A Gold Coast couple who funded a "lavish lifestyle" peddling MDMA and cannabis on the dark web is jailed after pleading guilty to drug trafficking.

Alexis Martin will soon be a free woman.

Increased microvascular permeability to plasma proteins and neutrophil emigration are hallmarks of innate immunity and key features of numerous inflammatory disorders. Although neutrophils can promote microvascular leakage, the impact of vascular permeability on neutrophil trafficking is unknown. Here, through the application of confocal intravital microscopy, we report that vascular permeability–enhancing stimuli caused a significant frequency of neutrophil reverse transendothelial cell migration (rTEM). Furthermore, mice with a selective defect in microvascular permeability enhancement (VEC-Y685F-ki) showed reduced incidence of neutrophil rTEM. Mechanistically, elevated vascular leakage promoted movement of interstitial chemokines into the bloodstream, a response that supported abluminal-to-luminal neutrophil TEM. Through development of an in vivo cell labeling method we provide direct evidence for the systemic dissemination of rTEM neutrophils, and showed them to exhibit an activated phenotype and be capable of trafficking to the lungs where their presence was aligned with regions of vascular injury. Collectively, we demonstrate that increased microvascular leakage reverses the localization of directional cues across venular walls, thus causing neutrophils engaged in diapedesis to reenter the systemic circulation. This cascade of events offers a mechanism to explain how local tissue inflammation and vascular permeability can induce downstream pathological effects in remote organs, most notably in the lungs.

L.A. Opera streams the musical drama "Angel's Bone," filmed last year in Beijing. It's your quarantine must-watch of the day.

Following its evoked release, dopamine (DA) signaling is rapidly terminated by presynaptic reuptake, mediated by the cocaine-sensitive DA transporter (DAT). DAT surface availability is dynamically regulated by endocytic trafficking, and direct protein kinase C (PKC) activation acutely diminishes DAT surface expression by accelerating DAT internalization. Previous cell line studies demonstrated that PKC-stimulated DAT endocytosis requires both Ack1 inactivation, which releases a DAT-specific endocytic brake, and the neuronal GTPase, Rit2, which binds DAT. However, it is unknown whether Rit2 is required for PKC-stimulated DAT endocytosis in DAergic terminals or whether there are region- and/or sex-dependent differences in PKC-stimulated DAT trafficking. Moreover, the mechanisms by which Rit2 controls PKC-stimulated DAT endocytosis are unknown. Here, we directly examined these important questions. Ex vivo studies revealed that PKC activation acutely decreased DAT surface expression selectively in ventral, but not dorsal, striatum. AAV-mediated, conditional Rit2 knockdown in DAergic neurons impacted baseline DAT surface:intracellular distribution in DAergic terminals from female ventral, but not dorsal, striatum. Further, Rit2 was required for PKC-stimulated DAT internalization in both male and female ventral striatum. FRET and surface pulldown studies in cell lines revealed that PKC activation drives DAT-Rit2 surface dissociation and that the DAT N terminus is required for both PKC-mediated DAT-Rit2 dissociation and DAT internalization. Finally, we found that Rit2 and Ack1 independently converge on DAT to facilitate PKC-stimulated DAT endocytosis. Together, our data provide greater insight into mechanisms that mediate PKC-regulated DAT internalization and reveal unexpected region-specific differences in PKC-stimulated DAT trafficking in bona fide DAergic terminals.

The respiratory epithelium comprises polarized cells at the interface between the environment and airway tissues. Polarized apical and basolateral protein secretions are a feature of airway epithelium homeostasis. Human respiratory syncytial virus (hRSV) is a major human pathogen that primarily targets the respiratory epithelium. However, the consequences of hRSV infection on epithelium secretome polarity and content remain poorly understood. To investigate the hRSV-associated apical and basolateral secretomes, a proteomics approach was combined with an ex vivo pediatric human airway epithelial (HAE) model of hRSV infection (data are available via ProteomeXchange and can be accessed at https://www.ebi.ac.uk/pride/ with identifier PXD013661). Following infection, a skewing of apical/basolateral abundance ratios was identified for several individual proteins. Novel modulators of neutrophil and lymphocyte activation (CXCL6, CSF3, SECTM1 or CXCL16), and antiviral proteins (BST2 or CEACAM1) were detected in infected, but not in uninfected cultures. Importantly, CXCL6, CXCL16, CSF3 were also detected in nasopharyngeal aspirates (NPA) from hRSV-infected infants but not healthy controls. Furthermore, the antiviral activity of CEACAM1 against RSV was confirmed in vitro using BEAS-2B cells. hRSV infection disrupted the polarity of the pediatric respiratory epithelial secretome and was associated with immune modulating proteins (CXCL6, CXCL16, CSF3) never linked with this virus before. In addition, the antiviral activity of CEACAM1 against hRSV had also never been previously characterized. This study, therefore, provides novel insights into RSV pathogenesis and endogenous antiviral responses in pediatric airway epithelium.

Following its evoked release, dopamine (DA) signaling is rapidly terminated by presynaptic reuptake, mediated by the cocaine-sensitive DA transporter (DAT). DAT surface availability is dynamically regulated by endocytic trafficking, and direct protein kinase C (PKC) activation acutely diminishes DAT surface expression by accelerating DAT internalization. Previous cell line studies demonstrated that PKC-stimulated DAT endocytosis requires both Ack1 inactivation, which releases a DAT-specific endocytic brake, and the neuronal GTPase, Rit2, which binds DAT. However, it is unknown whether Rit2 is required for PKC-stimulated DAT endocytosis in DAergic terminals or whether there are region- and/or sex-dependent differences in PKC-stimulated DAT trafficking. Moreover, the mechanisms by which Rit2 controls PKC-stimulated DAT endocytosis are unknown. Here, we directly examined these important questions. Ex vivo studies revealed that PKC activation acutely decreased DAT surface expression selectively in ventral, but not dorsal, striatum. AAV-mediated, conditional Rit2 knockdown in DAergic neurons impacted baseline DAT surface:intracellular distribution in DAergic terminals from female ventral, but not dorsal, striatum. Further, Rit2 was required for PKC-stimulated DAT internalization in both male and female ventral striatum. FRET and surface pulldown studies in cell lines revealed that PKC activation drives DAT-Rit2 surface dissociation and that the DAT N terminus is required for both PKC-mediated DAT-Rit2 dissociation and DAT internalization. Finally, we found that Rit2 and Ack1 independently converge on DAT to facilitate PKC-stimulated DAT endocytosis. Together, our data provide greater insight into mechanisms that mediate PKC-regulated DAT internalization and reveal unexpected region-specific differences in PKC-stimulated DAT trafficking in bona fide DAergic terminals.

The respiratory epithelium comprises polarized cells at the interface between the environment and airway tissues. Polarized apical and basolateral protein secretions are a feature of airway epithelium homeostasis. Human respiratory syncytial virus (hRSV) is a major human pathogen that primarily targets the respiratory epithelium. However, the consequences of hRSV infection on epithelium secretome polarity and content remain poorly understood. To investigate the hRSV-associated apical and basolateral secretomes, a proteomics approach was combined with an ex vivo pediatric human airway epithelial (HAE) model of hRSV infection (data are available via ProteomeXchange and can be accessed at https://www.ebi.ac.uk/pride/ with identifier PXD013661). Following infection, a skewing of apical/basolateral abundance ratios was identified for several individual proteins. Novel modulators of neutrophil and lymphocyte activation (CXCL6, CSF3, SECTM1 or CXCL16), and antiviral proteins (BST2 or CEACAM1) were detected in infected, but not in uninfected cultures. Importantly, CXCL6, CXCL16, CSF3 were also detected in nasopharyngeal aspirates (NPA) from hRSV-infected infants but not healthy controls. Furthermore, the antiviral activity of CEACAM1 against RSV was confirmed in vitro using BEAS-2B cells. hRSV infection disrupted the polarity of the pediatric respiratory epithelial secretome and was associated with immune modulating proteins (CXCL6, CXCL16, CSF3) never linked with this virus before. In addition, the antiviral activity of CEACAM1 against hRSV had also never been previously characterized. This study, therefore, provides novel insights into RSV pathogenesis and endogenous antiviral responses in pediatric airway epithelium.

apoB exists as apoB100 and apoB48, which are mainly found in hepatic VLDLs and intestinal chylomicrons, respectively. Elevated plasma levels of apoB-containing lipoproteins (Blps) contribute to coronary artery disease, diabetes, and other cardiometabolic conditions. Studying the mechanisms that drive the assembly, intracellular trafficking, secretion, and function of Blps remains challenging. Our understanding of the intracellular and intraorganism trafficking of Blps can be greatly enhanced, however, with the availability of fusion proteins that can help visualize Blp transport within cells and between tissues. We designed three plasmids expressing human apoB fluorescent fusion proteins: apoB48-GFP, apoB100-GFP, and apoB48-mCherry. In Cos-7 cells, transiently expressed fluorescent apoB proteins colocalized with calnexin and were only secreted if cells were cotransfected with microsomal triglyceride transfer protein. The secreted apoB-fusion proteins retained the fluorescent protein and were secreted as lipoproteins with flotation densities similar to plasma HDL and LDL. In a rat hepatoma McA-RH7777 cell line, the human apoB100 fusion protein was secreted as VLDL- and LDL-sized particles, and the apoB48 fusion proteins were secreted as LDL- and HDL-sized particles. To monitor lipoprotein trafficking in vivo, the apoB48-mCherry construct was transiently expressed in zebrafish larvae and was detected throughout the liver. These experiments show that the addition of fluorescent proteins to the C terminus of apoB does not disrupt their assembly, localization, secretion, or endocytosis. The availability of fluorescently labeled apoB proteins will facilitate the exploration of the assembly, degradation, and transport of Blps and help to identify novel compounds that interfere with these processes via high-throughput screening.

Shane Rea
Nov 1, 1997; 46:1667-1677
Perspectives in Diabetes

Human trafficking.

Items recovered include fossils, paintings, ancient coins, ceramics and jewelry

For 10 years she was forced into prostitution by her husband. With the help of OM Mexico, Rocio is now building a new life.

A search warrant executed by Hamilton police vice and drugs officers at an address in the city’s east end has resulted in the arrest of a couple and multiple charges, police said in a Friday release.

For 10 years she was forced into prostitution by her husband. With the help of OM Mexico, Rocio is now building a new life.

Existing literature discusses the unique medical and psychological needs of sex trafficking victims and highlights the importance of screening patients with risk factors. However, little is known about providers’ knowledge and confidence in their ability to provide care to victims.

The study summarizes the knowledge gaps and barriers providers face when assisting pediatric sex trafficking victims. It also highlights the impact of training on providers’ confidence and ability to appropriately care for victims. (Read the full article)