In order to make its employees more comfortable when they want to take a break from work, a sports marketing company from Sao Paulo, Latin Sports, asked Brazili

The fast-melting Thwaites Glacier won't be able to hold back a massive flood of ice water.

Researchers unveil the most detailed map of what's underneath the Antarctic ice sheet, and it will help them predict climate change vulnerability there.

Disclosed herein is a method of delineating a second wellbore from a first wellbore. The method includes, emitting acoustic waves from a tool in the first wellbore, receiving acoustic waves at the tool reflected from the second wellbore, and determining orientation and distance of at least a portion of the second wellbore relative to the tool.

Trevor Ashby's property south of Mount Gambier looks like a typical dairy farm from the roadside, but hidden among the cows is a tiny portal into a world-class dive site.

Majok Tulba

Fire crews have spent more than two weeks extinguishing a coal wash fire burning underground on a property in NSW.

Residents in one of Australia's most famous gold rush towns are divided over plans to explore for the precious metal beneath its streets, amid record prices for gold which have injected new life into Coolgardie.

What does the legendary leg-spinner have in common with an ancient volcano range beneath a remote stretch of outback SA and Queensland? A "fiery temperament" as well as "explosive talent", a geoscientist says.

Yolngu people in north-east Arnhem Land consider white ochre sacred and source it to use in ceremonies and art works. A recent discovery beneath a community art centre has the Yirrkala community celebrating.

The post Fishing Footwear Basics: Keeping Your Feet Neat and Safe appeared first on Ocean Blue Fishing Adventures.

Thwaites Glacier is melting fast. But to understand how climate change is driving its decline, scientists need to send instruments through 2,000 feet of ice into the water below.

ARCHAEOLOGISTS have discovered a long lost mass grave of US Civil War soldiers in Mississippi.

12 December 2016

T-type (Cav3) Ca2+ channels are important regulators of excitability and rhythmic activity of excitable cells. Among other voltage-gated Ca2+ channels, Cav3 channels are uniquely sensitive to oxidation and zinc. Using recombinant protein expression in HEK293 cells, patch clamp electrophysiology, site-directed mutagenesis, and homology modeling, we report here that modulation of Cav3.2 by redox agents and zinc is mediated by a unique extracellular module containing a high-affinity metal-binding site formed by the extracellular IS1–IS2 and IS3–IS4 loops of domain I and a cluster of extracellular cysteines in the IS1–IS2 loop. Patch clamp recording of recombinant Cav3.2 currents revealed that two cysteine-modifying agents, sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES) and N-ethylmaleimide, as well as a reactive oxygen species–producing neuropeptide, substance P (SP), inhibit Cav3.2 current to similar degrees and that this inhibition is reversed by a reducing agent and a zinc chelator. Pre-application of MTSES prevented further SP-mediated current inhibition. Substitution of the zinc-binding residue His191 in Cav3.2 reduced the channel's sensitivity to MTSES, and introduction of the corresponding histidine into Cav3.1 sensitized it to MTSES. Removal of extracellular cysteines from the IS1–IS2 loop of Cav3.2 reduced its sensitivity to MTSES and SP. We hypothesize that oxidative modification of IS1–IS2 loop cysteines induces allosteric changes in the zinc-binding site of Cav3.2 so that it becomes sensitive to ambient zinc.

T-type (Cav3) Ca2+ channels are important regulators of excitability and rhythmic activity of excitable cells. Among other voltage-gated Ca2+ channels, Cav3 channels are uniquely sensitive to oxidation and zinc. Using recombinant protein expression in HEK293 cells, patch clamp electrophysiology, site-directed mutagenesis, and homology modeling, we report here that modulation of Cav3.2 by redox agents and zinc is mediated by a unique extracellular module containing a high-affinity metal-binding site formed by the extracellular IS1–IS2 and IS3–IS4 loops of domain I and a cluster of extracellular cysteines in the IS1–IS2 loop. Patch clamp recording of recombinant Cav3.2 currents revealed that two cysteine-modifying agents, sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES) and N-ethylmaleimide, as well as a reactive oxygen species–producing neuropeptide, substance P (SP), inhibit Cav3.2 current to similar degrees and that this inhibition is reversed by a reducing agent and a zinc chelator. Pre-application of MTSES prevented further SP-mediated current inhibition. Substitution of the zinc-binding residue His191 in Cav3.2 reduced the channel's sensitivity to MTSES, and introduction of the corresponding histidine into Cav3.1 sensitized it to MTSES. Removal of extracellular cysteines from the IS1–IS2 loop of Cav3.2 reduced its sensitivity to MTSES and SP. We hypothesize that oxidative modification of IS1–IS2 loop cysteines induces allosteric changes in the zinc-binding site of Cav3.2 so that it becomes sensitive to ambient zinc.

T-type (Cav3) Ca2+ channels are important regulators of excitability and rhythmic activity of excitable cells. Among other voltage-gated Ca2+ channels, Cav3 channels are uniquely sensitive to oxidation and zinc. Using recombinant protein expression in HEK293 cells, patch clamp electrophysiology, site-directed mutagenesis, and homology modeling, we report here that modulation of Cav3.2 by redox agents and zinc is mediated by a unique extracellular module containing a high-affinity metal-binding site formed by the extracellular IS1–IS2 and IS3–IS4 loops of domain I and a cluster of extracellular cysteines in the IS1–IS2 loop. Patch clamp recording of recombinant Cav3.2 currents revealed that two cysteine-modifying agents, sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES) and N-ethylmaleimide, as well as a reactive oxygen species–producing neuropeptide, substance P (SP), inhibit Cav3.2 current to similar degrees and that this inhibition is reversed by a reducing agent and a zinc chelator. Pre-application of MTSES prevented further SP-mediated current inhibition. Substitution of the zinc-binding residue His191 in Cav3.2 reduced the channel's sensitivity to MTSES, and introduction of the corresponding histidine into Cav3.1 sensitized it to MTSES. Removal of extracellular cysteines from the IS1–IS2 loop of Cav3.2 reduced its sensitivity to MTSES and SP. We hypothesize that oxidative modification of IS1–IS2 loop cysteines induces allosteric changes in the zinc-binding site of Cav3.2 so that it becomes sensitive to ambient zinc.

The Beaker man was buried alongside four cowhide "rugs," an eight-inch copper dagger and a wrist guard made of rare green stone

Wood used to build the secret passageway came from a tree felled in 1874, according to a new analysis

A prototype deployed in San Francisco Bay imagines the underside of a floating building as an upside-down artificial reef

Three OM Hungary team members share what they’ve learned while serving as teachers at the International Christian School of Budapest.

NEATEBOX, the Scottish technology firm which specialises in improving accessibility for people with disabilities, has been accepted into an accelerator programme run by Royal Bank of Scotland.

On Saturday, January 4, 10:30 a.m., President Robert Clark of Wesley College will speak at the Delaware Public Archives on the role of Submarine Operations during World War II.

The post War Beneath the Waves: U.S. Submarine Operations in World War II appeared first on Delaware Public Archives - State of Delaware.

No matter how deep we dig, life has always found a way to survive. The remarkable story of these impossible microbes can teach us about how life evolved

Asja Guzman, Rachel C. Avard, Alexander J. Devanny, Oh Sang Kweon, and Laura J. Kaufman

The study of cancer cell invasion in 3D environments in vitro has revealed a variety of invasive modes, including amoeboid migration, characterized by primarily round cells that invade in a protease- and adhesion-independent manner. Here, we delineate a contractility-dependent migratory mode of primarily round breast cancer cells that is associated with extensive integrin-mediated extracellular matrix (ECM) reorganization that occurs at membrane blebs, with bleb necks sites of integrin clustering and integrin-dependent ECM alignment. We show that the spatiotemporal distribution of blebs and their utilization for ECM reorganization is mediated by functional β1 integrin receptors and other components of focal adhesions. Taken together, the work presented here characterizes a migratory mode of primarily round cancer cells in complex 3D environments and reveals a fundamentally new function for membrane blebs in cancer cell invasion.

China’s Yutu-2 rover has used radar to peer 40 metres under the surface of the far side of the moon and revealed how past impacts have shaped its geology

What lies inside the icy cavern seems more and more like a captive, rare animal, an Earth form that might soon be lost

Scientists have been studying the huge submerged landmass for decades and are now pushing for its recognition as a continent.

A roundup of some posts from our sister site.

Not in the ocean. Below it.

Forest ecosystems sequester large amounts of carbon in vegetation and soils, off-setting the carbon emissions we produce and mitigating climate change. The boreal forest and its associated wetlands, in particular, provide critical carbon storage ...

Another product from Colombian studio DosUno Design (whose Rubix transformer furniture set we reviewed yesterday), Deckstool is a simple wood mat that folds into a stool. Apart from being perfect for small spaces,

Who needs concrete when this foundation design will take the load?

Stocks were down slightly on Tuesday, but beneath the surface lies the continuation of a powerful rally.

Last week, Kim Kardashian gave her 160 million Instagram followers a behind-the-scenes look at her children's playroom - and fans were shocked to see how incredibly neat and orderly it is.

Laverne Cox looked incredible as she joined Gigi Gorgeous and Nats Getty at the Los Angeles Lakers and Golden State Warriors basketball game on Wednesday.

Joe Jonas and his pregnant wife Sophie Turner wore face masks, as they emerged for the first time in nearly a month to walk their beloved pups on Thursday in Los Angeles.

Alfredo Morelos' car is being examined by the police after a man was allegedly discovered to be tampering with the Ranger's striker's vehicle on Tuesday night.

The Hollywood Reporter claimed on Monday that she has in talks to take the lead role in crime thriller The Ice Beneath Her.

Irina Shayk turned New York City into her own private catwalk on Thursday, when the 34-year-old model went for a stroll with three-year-old daughter Lea.

The 35-year-old singer went incognito underneath a hooded sweatshirt paired with leggings after a private sweat session with her pet pooches in tow.

Kendall Jenner seemed so excited to get to Ben Simmons game against the Miami Heat, Monday night she missed a button on her sheer brown blouse.

Kate Hudson appeared to make the most of her down time, with the Academy Award nominated actress stepping out for a stroll around California's Pacific Palisades on Friday.

The supermodel, 33, was making a style statement in a silk suit with her black bra on show from underneath the open blazer while she kept her hair and make-up pared back

Iran's judicial spokesman Gholam Hossein Esmaili revealed the new figures Tuesday as he vowed to punish the 'criminals' responsible for peddling the fake coronavirus 'cure'.

A decadent swimming pool set in the middle of an Australian mansion is hiding an underground wine lounge with a 3,000-bottle cellar, designed with a day bed and window that looks out on the water.

It seems Victoria Beckham has evened the score between her and husband David, with her latest Instagram post.

Vichhoda moves through the bylanes of Kashmir and reaches corruption-ridden small towns of Pakistan to seek humanity in the most unexpected places.

Browsery TA712.H86 2018