Chinook salmon spark excitement among local Klamath Tribes, who have advocated for decades to restore the flow of the river in California and Oregon

The so-called Chew Valley Hoard, which just sold for a record $5.6 million, includes 2,584 coins featuring the likenesses of Harold II, William the Conqueror and Edward the Confessor

The new research could change history's understanding of the sprawling trade network that connected Europe and the Middle East to East Asia

A "staggering array" of markings have been hiding in plain sight carved into the walls of Gainsborough Old Hall, a 500-year-old home in Lincolnshire, England

The species may have been carried to the Sacramento–San Joaquin Delta in ballast water on ships

In a rare technique among mammals, the bats burn proteins from blood, rather than carbs or fat, to power their pursuits of prey, according to a new study

This story and others have come to light during a project to translate and digitize a series of texts about archbishops in York, England

In the 1960s, a vaccine for chlamydia made patients more susceptible to chlamydia. Now scientists know why

A century on, the country’s most beloved Thursday spectacle reaches new heights

Shallaway Youth Choir is bringing a program of music to the Basilica in downtown St. John’s on Remembrance Day. Artistic director Kellie Walsh says the choir will sing about such topics as the Unknown Soldier and schoolchildren who care for Newfoundland graves in London. Where Once They Stood will be performed at 2 p.m. and 7 p.m. on Monday.

Pat White, an entrepreneur who launched EVAS Air and the Gander Flight Training, has died. He was 69 years old.

A candidate for the Nova Scotia NDP has apologized and is no longer running for the party in the provincial election after her comments on Israel and the Holocaust drew concern from the Jewish organizations. 

The only daily TV news package to focus on Nova Scotians and their stories

The only daily TV news package to focus on Nova Scotians and their stories

Some Nova Scotia municipal leaders are defending a funding stream the federal Conservatives say they will cut if elected, calling it a "game changer."

Laboratory study of field-collected mosquitoes can allow researchers to better understand the ways variation within and among mosquito populations shapes burdens of mosquito-borne disease. The Anopheles gambiae complex comprises the most important vectors of malaria, but it can be challenging to keep in the laboratory. For some species of mosquitoes, especially An. gambiae, it is very difficult to bring viable eggs into the laboratory. Instead, it is preferable to collect larvae or pupae and then transport them as carefully as possible back to the laboratory. This simple protocol allows a researcher to start new laboratory colonies from larvae or pupae collected from natural breeding sites or proceed directly to their planned experiments. The use of natural breeding sites provides additional reassurance that the resulting colonies are representative of natural populations.

Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.

All PCR- and DNA-based blood meal analyses require host DNA from a mosquito blood meal to be effectively preserved between the time when the specimen is collected and the extraction of DNA. As soon as a mosquito ingests blood from a host animal, digestion of host cells and cellular components within the blood meal by enzymes in the mosquito midgut begins to degrade the host DNA templates that are the targets of polymerase chain reaction (PCR) amplification. Without effective preservation, host DNA is typically undetectable by PCR 48 h after feeding, because of digestion. Preservation methods for mosquito blood meals vary in their efficacy, and the logistics of fieldwork can limit the options for preservation of blood meals and maintenance of the integrity of host DNA. This protocol describes a method of blood meal preservation that is effective, convenient, and amenable to fieldwork in remote locations where cryopreservation at –20°C or –80°C may not be feasible. It uses a Flinders Technology Associates (FTA) card, which is a chemically treated card that lyses cells and allows nucleic acids to be preserved. This method is also expected to preserve the DNA or RNA of pathogens present within the engorged mosquito abdomen, including RNA viruses.

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

Yukon appeal court Justice Karen Horsman granted a temporary stay of the requirement on Friday, the latest development in an ongoing legal battle over the property between the territorial government, cabin-owner Len Tarka and tenant Eric DeLong. 

The bronze wreath immortalized the moment when the members of the Honor Guard removed their hats and placed them on the president's grave during his burial

The British explorer named dozens of geographical features and sites in the region, ignoring the traditions of the Indigenous peoples who’d lived there for millennia

As a child, the future president acquired a marine animal's skull, which became the first specimen in his natural history collection

A new exhibition at the British Library explores the public, private and spiritual lives of such figures as Joan of Arc, Christine de Pizan and Hildegard of Bingen

Arrival Day brought teens, Service Team members, coaches and thunderstorms. As another incredible TeenStreet began, teens shared what they are most looking forward to in the week ahead.

The P.E.I. Woodlot Owners Association is encouraging its members to boost the biodiversity of their woods with different varieties of trees and shrubs. As CBC's Nancy Russell reports, the goal is to provide habitat for endangered species and protect the forests from future disasters like post-tropical storm Fiona.

The entrepreneur and financial adviser defeated Adam Bassett for the party nomination in Egmont after two days of voting last week. 

The Prince Edward Island Fire Marshals Office has concluded its investigation into a massive fire at Valley Truss and Metal in Kensington in October.

Cavalry FC defender Daan Klomp who considered quitting soccer in the Netherlands in 2020, will go after the Canadian Premier League title Saturday against defending champion Forge FC. And his trophy case may grow a little fuller Thursday at the CPL awards show.

Calgary's Cavalry FC could win its first Canadian Premier League championship on Saturday when it takes on Hamilton's Forge FC in front of a home crowd at ATCO field.

LAFC opened the series with a narrow 2-1 victory in California and Vancouver replied with a decisive 3-0 win at home to set up a do-or-die matchup on Friday, but they failed to overcome L.A. and suffered a 1-0 defeat.

Calgary’s Cavalry FC can finally call itself Canadian Premier League champions. Playing in front of its hometown crowd, Cavalry FC dethroned the four-time champions Forge FC from Hamilton 2-1 Saturday in Calgary.

The Winnipeg Blue Bombers will face the Toronto Argonauts in their fifth straight Grey Cup appearance. The Bombers advanced to the CFL title game after disposing of the Saskatchewan Roughriders 38-22 in the West final Saturday night in Winnipeg.

It was only the second time the Saskatchewan Huskies and the Regina Rams have met in the Hardy Cup championship game. This time it was the Rams coming out on top 19-14. Huskies head coach Scott Flory says there were too many turnovers and too many plays left on the field after a hard fought game.

A blaze at an apartment building has displaced residents and led to two injuries, almost two years after a fire at the same building displaced dozens.

Several firearms, body armour, diamonds and fentanyl shaped like dog treats were among the items seized in a recent bust, say Metro Vancouver Transit Police.

More than 14 months after flooding caused by heavy rainfall forced the Ottawa Birth and Wellness Centre to shut down its birthing unit, it's once again welcoming expecting families.

Health officials say it’s the right time to get your seasonal flu and COVID-19 vaccines as levels of both viruses rise in Ottawa. The CBC’s Kimberley Molina reports.

Vanessa James and Eric Radford withdrew from the pairs competition at the Canadian figure skating championships before Saturday's free program in Ottawa.

Windsor's push to tax people that own houses that sit empty in a city dealing with an affordable housing crunch has led to some surprising early results.

The Edmonton Oilers may have found the break they were looking for. After struggling through the first month of the NHL season, the Oilers trounced the Vancouver Canucks 7-3 on Saturday.

U.S. president-elect Donald Trump said on Tuesday he was nominating former Arkansas governor Mike Huckabee as its next ambassador to Israel, tapping a staunchly pro-Israel conservative whose choice could signal future U.S. policy toward conflicts in the Middle East.

Toronto's redesigned vacant home tax rollout has begun, with over 59,000 people filing their paperwork in the first week. Some councillors say they were left responding to complaints about the rollout, after the city's online declaration portal experienced technical problems shortly after launch.

The Calgary-based team beat Hamilton Forge FC in a 2-1 nail-biter at home on Saturday, in front of a sold-out crowd of over 7,000 fans at Spruce Meadows.

CME Group, the world's leading derivatives marketplace, today announced it has received approval from the National Futures Association (NFA) to establish a futures commission merchant (FCM). "We...

CRISPR–Cas9 has revolutionized gene editing for traditional and nontraditional model organisms alike. This tool has opened the door to new mechanistic studies of basic mosquito biology as well as the development of novel vector control strategies based on CRISPR–Cas9, including gene drives that spread genetic elements in the population. Although the promise of the specificity, flexibility, and ease of deployment CRISPR is real, its implementation still requires empirical optimization for each new species of interest, as well as to each genomic target within a given species. Here, we provide an overview of designing and testing single-guide RNAs for the use of CRISPR-based gene editing tools.

Knee replacement doesn’t have to be ‘total’

With 3D CAD, Company Reduces Design Cycle from Three Weeks to Three or Four Days

SolidWorks World 2009 Wraps Up Exhilarating Week of Learning and Networking