Sailing across the Straits of Gibraltar to North Africa. On a course to get my International Yacht Skipper Licence. Sailing to three countries and two continents in a week. I passed the test so now I'm now an officially licenced Skipper Who's up for a s

Para ayudarlo a planificar su estada he preparado este itinerario detallado . Durante su viaje podr visitar Valletta la capital as como Mdina Rabat los acantilados de Dingli o incluso algunas de las playas ms bonitas

We left HCM at 8.30 by taxi and arrived in Mekong Delta around midday. We met our guide Nam and boarded a long wooden boat to take us down the river making several stops along the way.The first stop was a brick factory where Nam told me about how bri

A 14-second video captured the unsettling scene inside St. Anthony of Padua Church in Greenpoint as the Rev. Jossy Vattothu presided over the 9:30 a.m. Mass, with the man strolling casually inside the house of worship with a container of juice in his right hand.

Cuirfear tús le cainteanna foirmiúla tráthnóna idir trí pháirtí polaitíochta - Fine Gael, Fianna Fáil agus an Comhaontas Glas - maidir le bunú rialtais nua.

The West Adams restaurant Alta Adams reopens with a sliding price scale so people in need can dine for free.

TWENTY-three British citizens left stranded in Morocco as a result of the coronavirus pandemic have been returned to their homes in Gibraltar on two specially commissioned vessels.

Roles & Responsibilities Document test methods, perform system testing, and document test results for HV system performance testing Lab development testing and documentation of power electronic subsystems Testing of high voltage, high power electronic components including system bring up an

Roles & Responsibilities: Design and optimization of HV circuits (450 to 1700 V) including gate drives, inverter, and protection features for low and high power circuits Create and document models for HV circuits Develop worst case analysis for HV Circuits Create detailed component desi

Delta said it is making the move to protect employees amid the coronavirus pandemic, but planes have been flying near empty

Infectologista da organização explica que células mortas expelidas do pulmão fazem exames darem positivo mais de uma vez — mas não necessariamente quer dizer que a pessoa voltou a ficar infectada pelo coronavírus.

Em estudo clínico randomizado controlado, pessoas que receberam as substâncias interferon beta 1-b, lopinavir-ritonavir e ribavirin tiveram tempo menor para alta e desaparecimento do vírus, na comparação com o grupo controle.

Profissionais de enfermagem relatam falta de equipamentos de proteção, baixas nas equipes e lotação; em ofício, governador Witzel reconhece que esforços contra a covid-19 não foram suficientes e que estuda endurecer confinamento.

We are now quite familiar with customer-written hotel reviews; we also know about the trendy use of video reviews of hotels but what about reviews of flights?! Yesterday I stumbled upon an incredible amateur video that made it to the top #20 of the most watched video of Youtube. The statistics speak for themselves: Views: 76,062 Comments: 468 Favorited: [...]

A chronology of key events

A chronology of key events

Key facts, figures and dates

An overview of Gibraltar including key facts, political leaders and notes on the media

Washington VIPs (hint: allll the Housewives) showed up for Cirque's ode to street sports, "Volta," which plays at Tysons II through Sept. 29.

He just took out two birds with one proclamation.

Malta

The Amateur Traveler talks to San Francisco Chronicle Travel Editor Spud Hilton about Gibraltar. This little patch of England at the bottom of Spain is more than just a place where you can see a large rock and get good fish and chips with your tapas. Explore the "rock" and meet the "apes" of Gibraltar. Hear about some of the history as well as the unique traffic challenges of this tiny peninsula. Also learn about Winston Churchill's secret World War 2 monkey plan for Gibraltar. Along the way let me introduce you to Spud Hilton who is one of my favorite travel editors.

Hear about travel to the Danube Delta in Romania as the Amateur Traveler talks to Christian Cummins about traveling to this UNESCO biosphere in Europe.

RCMP say a 50-year-old man died and a 60-year-old man is in hospital following a crash involving a CP Rail train Friday morning.

Cochrane RCMP say charges have been laid against two individuals in connection with an investigation that began with a firearms complaint.

The Vuelta a Espana organisers have confirmed that a race already rescheduled and shortened would also now cut its foray into neighbouring Portugal from the 2020 itinerary.

I was excited to finally get the word that an article I wrote sharing my love for my hometown – San Jose, California – is out in Delta Sky Magazine this month! Time Out: San José (Are you flying Delta this month? If so, pick up a copy for me! I’d love to see it […]

The article EIT Elsewhere | “Time Out: San José” in Delta Sky Magazine originated at EverInTransit.com

16 January 2020

Protein phosphatase 2A (PP2A) is a large enzyme family responsible for most cellular Ser/Thr dephosphorylation events. PP2A substrate specificity, localization, and regulation by second messengers rely on more than a dozen regulatory subunits (including B/R2, B'/R5, and B″/R3), which form the PP2A heterotrimeric holoenzyme by associating with a dimer comprising scaffolding (A) and catalytic (C) subunits. Because of partial redundancy and high endogenous expression of PP2A holoenzymes, traditional approaches of overexpressing, knocking down, or knocking out PP2A regulatory subunits have yielded only limited insights into their biological roles and substrates. To this end, here we sought to reduce the complexity of cellular PP2A holoenzymes. We used tetracycline-inducible expression of pairs of scaffolding and regulatory subunits with complementary charge-reversal substitutions in their interaction interfaces. For each of the three regulatory subunit families, we engineered A/B charge–swap variants that could bind to one another, but not to endogenous A and B subunits. Because endogenous Aα was targeted by a co-induced shRNA, endogenous B subunits were rapidly degraded, resulting in expression of predominantly a single PP2A heterotrimer composed of the A/B charge–swap pair and the endogenous catalytic subunit. Using B'δ/PPP2R5D, we show that PP2A complexity reduction, but not PP2A overexpression, reveals a role of this holoenzyme in suppression of extracellular signal–regulated kinase signaling and protein kinase A substrate dephosphorylation. When combined with global phosphoproteomics, the PP2A/B'δ reduction approach identified consensus dephosphorylation motifs in its substrates and suggested that residues surrounding the phosphorylation site play roles in PP2A substrate specificity.

Protein phosphatase 2A (PP2A) is a large enzyme family responsible for most cellular Ser/Thr dephosphorylation events. PP2A substrate specificity, localization, and regulation by second messengers rely on more than a dozen regulatory subunits (including B/R2, B'/R5, and B″/R3), which form the PP2A heterotrimeric holoenzyme by associating with a dimer comprising scaffolding (A) and catalytic (C) subunits. Because of partial redundancy and high endogenous expression of PP2A holoenzymes, traditional approaches of overexpressing, knocking down, or knocking out PP2A regulatory subunits have yielded only limited insights into their biological roles and substrates. To this end, here we sought to reduce the complexity of cellular PP2A holoenzymes. We used tetracycline-inducible expression of pairs of scaffolding and regulatory subunits with complementary charge-reversal substitutions in their interaction interfaces. For each of the three regulatory subunit families, we engineered A/B charge–swap variants that could bind to one another, but not to endogenous A and B subunits. Because endogenous Aα was targeted by a co-induced shRNA, endogenous B subunits were rapidly degraded, resulting in expression of predominantly a single PP2A heterotrimer composed of the A/B charge–swap pair and the endogenous catalytic subunit. Using B'δ/PPP2R5D, we show that PP2A complexity reduction, but not PP2A overexpression, reveals a role of this holoenzyme in suppression of extracellular signal–regulated kinase signaling and protein kinase A substrate dephosphorylation. When combined with global phosphoproteomics, the PP2A/B'δ reduction approach identified consensus dephosphorylation motifs in its substrates and suggested that residues surrounding the phosphorylation site play roles in PP2A substrate specificity.